Professional Documents
Culture Documents
IC Applications .. .. .. .. .. .. .. .. .. .. .. .. ..514-521
Anions .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..514-518
Cations .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..519-520
Surfactants .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 521
GC Applications .. .. .. .. .. .. .. .. .. .. .. ..522-556
Drugs .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..522-528
Environmental .. .. .. .. .. .. .. .. .. .. .. .. .. ..528-536
Foods, Flavors & Fragrances .. .. .. .. .. .. ..537-542
Petroleum & Petrochemical .. .. .. .. .. .. ..542-547
Solvents & Chemicals .. .. .. .. .. .. .. .. .. ..547-556
Index .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..557-576
Applications Index .. .. .. .. .. .. .. .. .. .. .. ..557-564
Product Index .. .. .. .. .. .. .. .. .. .. .. .. .. ..565-572
Offices and Distributors .. .. .. .. .. .. .. .. ..573-576
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
481
PHARMACEUTICALS
HPLC Applications
Analgesics
Anti-Inflammatories Anti-Inflammatories
�
8156 � 10158
�
1. Naproxen 1. Fenoprofen
� �
2. Acemetacin 2. Flurbiprofen
3. Indomethacin 3. Ketoprofen
� 4. p-Xylene
� � � �
� � � � � ������� � � � � � �� �������
Column: Platinum™ C18, 5µm, 150 x 4.6mm Column: Inertsil® Diol, 5µm, 250 x 4.6mm
(Part No. 32043) (Part No. GL-05646)
Mobile Phase: 0.02M KH2PO4:Methanol:Acetonitrile: Mobile Phase: Hexane:Ethanol (90:10)
pH4.5 (40:50:10) Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254
Detector: UV at 254nm
Analgesics �
Analgesics
10124 9385
��
��
1. Aspirin 1. Aspirin
�� 2. Acetaminophen � 2. Acetaminophen
3. Naproxen 3. Naproxen
4. Fenprofen 4. Fenoprofen
5. Ibuprofen � �
�� ��
6. Diclofenac
��
� � � � � �������
� � � � � �� �� �������
Column: Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072)
™
Column: Prevail™ Amide, 5µm, 150 x 4.6mm
Mobile Phase: A: 0.05M KH2PO4 pH3.3 B: Methanol C: Acetonitrile (Part No. 88660)
Gradient: Time: 0 2 5 Mobile Phase: 0.02M K2HPO4 pH7.0:Acetonitrile (75:25)
%B: 10 10 30 Flow Rate: 1.0mL/min
%C: 10 10 25 Detector: UV at 210nm
Flow Rate: 2.0mL/min
Detector: UV at 280nm
1. Procainimide
2. Acetaminophen CH3
3. Theophylline
1 4. Caffeine
CHCOH
3
4 O
H3CO
0 3 6 9 12 15 Min. � � �� �������
Column: Alltima™ HP C8, 5µm, 150 x 4.6mm Column: Chirobiotic V™, 250 x 4.6mm (Part No. 11024)
(Part No. 87751) Mobile Phase: 0.1% Triethylammonium Acetate (TEAA),
Mobile Phase: 0.05M Phosphate Buffer pH3.0:Methanol (80:20) pH7:THF (90:10)
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 254nm Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Anti-Depressants
0 1 3 2 4 5 6 7 8 Min. � � � � � �� �� �� �� �� �������
Column: Alltima™ CN, 3µm, 150 x 4.6mm Column: Alltima™ Silica, 5µm, 300 x 4.6mm (Custom)
(Part No. 81189) Mobile Phase: CH3CN:CHCl3:i-BuOH:Water:Glacial HOAc (85:8:5:2:0.05)
Mobile Phase: 0.01M KH2PO4 pH2.6:Acetonitrile (50:50) Flow Rate: 2.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254nm
Detector: UV at 254nm
Basic Drugs Tricyclic Antidepressants
10353 10373
1
1. Diphenhydramine 1. Desipramine
2 4
� 2. Nortriptyline 2. Nortryptyline
3. Amitriptyline 3. Diphenhydramine
� 4. Amitriptyline
�
3
� � � � � � � � � ������ 0 2 4 6 8 10 12 14 16 18 20 22 Min.
Column: Alltima HP C18 Amide, 5µm, 150 x 4.6mm
™
Column: Alltima™ HP C8, 5µm, 150 x 4.6mm
(Part No. 87733) (Part No. 87751)
Mobile Phase: 0.025M Phosphate Buffer pH7:Methanol (15:85) Mobile Phase: A: 0.025M Phosphate Buffer pH7 B: Methanol
Flow Rate: 1mL/min Gradient: Time 0 8 20
Detector: UV at 254nm %B 60 75 75
Flow Rate: 1.0mL/min
Detector: UV at 254nm
� � � ������ 0 1 2 3 4 5 6 Min.
Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ (Part No. 50605)
(Part No. 50573) Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Mobile Phase: 0.05M Ammonium Acetate pH5.5:Acetonitrile Gradient: Time: 0 6
(65:35) %B: 10 90
Flow Rate: 3.0mL/min Flow Rate: 2.5mL/min
Detector: UV at 254nm Detector: ELSD
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Anti-Asthmatics
Methylxanthines Theophylline & Metabolites
3 8384 8679
1 2 1. Theobromine 1. Xanthine
2. Theophylline 2. Theobromine
3. 8-Chlorotheophylline 3. Theophylline
4 1
5 4. Caffeine 4. Caffeine
5. 7-(2-Chloroethyl) 5. 8-Chlorotheophylline
theophylline 6. 7-(2-chloroethyl)theophylline
2
3 4 5
6
0 2 4 6 8 10 Min. 0 1 2 3 Min.
Column: Platinum™ EPS C18, 100Å, 5µm, 150 x 4.6mm Column: Platinum™ EPS C18, 3µm, 33 x 7mm Rocket™
(Part No. 32214) (Part No. 50575)
Mobile Phase: Water:Acetonitrile:Methanol: Glacial Acetic Acid Mobile Phase: 0.010M Sodium Acetate pH4.0:Methanol (70:30)
(60:10:30:1) Flow Rate: 5.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254nm
Detector: UV at 254nm
Barbiturates
2 Barbiturate Mix Barbiturate Mix
10375 8684
1. Barbital 1. Barbital
5 2. Phenobarbital 2. Butalbarbital
3. Talbutal 3. Aprobarbital
4. Amobarbital 4. Talbutal
1
5. Mephobarbital 5. Butabarbital
3 8 6. Phenobarbital
1 7. Amobarbital
3 2 8. Mephobarbital
4 5 6
4 7
0 2 4 6 8 10 12 14 16 18 20 Min. 0 1 2 3 4 Min.
Column: Alltima™ HP C8, 5µm, 250 x 4.6mm Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™
(Part No. 87751) (Part No. 50575)
Mobile Phase: 0.05M Ammonium Acetate:Acetonitrile (78:22) Mobile Phase: 0.01M Sodium Acetate pH4.0:Acetonitrile (75:25)
Flow Rate: 1.0mL/min Flow Rate: 3.0mL/min
Detector: UV at 230nm Detector: UV at 230nm
Sedatives Anesthetic
� Sedatives/Hypnotics Anesthetic Mix
8160 � 10118
� � � � � �� �������
Antihistamines
Antihistamines � Antihistamines
4 7232 10163
��
3 5 1. Tripelennamine 1. Tripelennamine
2. Triprolidine 2. Triprolidine
�
2 3. Cyclizine � 3. Cyclizine
4. Chlorcyclizine 4. Pyrrobutamine Phosphate
1 5. Meclizine � 5. Meclizine
0 5 10 15 20 25 Min. � � � � � �� ������
Column: Alltima™ C8, 5µm, 250 x 4.6mm (Part No. 88076) Column: ZirChrom® PS, 3µm, 50 x 4.6mm (Part No. ZR09-0546)
Mobile Phase: A: 0.05M Potassium Phosphate, pH3.2 Mobile Phase: A: Acetonitrile
B: Acetonitrile B: 25mM Hydrochloric Acid
Gradient: Time: 0 5 20 25 Gradient: Time: 0 1.0 1.1 4.0
%B: 25 25 70 70 %B: 95 85 80 40
Flow Rate: 1.3mL/min Flow Rate: 1.0mL/min
Detector: UV at 220nm Detector: UV at 254
� � � � � �� �� �� ������� 0 5 10 Min.
Column: ZORBAX® SB-Aq, 5µm, 150 x 4.6mm Column: ZORBAX® Extend-C18, 5µm, 150 x 4.6mm
(Part No. 883975-914) (Part No. 773450-902)
Mobile Phase: 0.2% TFA:Acetonitrile (90:10) Mobile Phase: 0.02M Triethylamine:Methanol (30:70)
Flow Rate: 1.5 mL/min Flow Rate: 1.0mL/min
Column Temp: 25˚C Detector: UV at 254nm
Detector: UV at 254nm
� �
�� �� ��
�� �� ��������������� � � � � � � � � � � �� �������
Column: ZORBAX® Bonus-RP, 5µm, 150 x 4.6mm Column: Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
™
Anticonvulsants
Anticonvulsants Flavonoids
9394 10164
�
3
1. Ethotoin 1. Primidone
�
2. Mephenytoin 2. Metharbital
3. Carbamazepine 3. Mephenytoin
4 4. Diazepam 4. Phenobarbital
� �
5. Phenytoin
2
1 �
0 2 4 6 8 Min. � � � � � �� �������
Column: Prevail™ Phenyl, 5µm, 150 x 4.6mm Column: ZirChrom® DiamondBond™-C18, 3µm, 100 x 4.6mm
(Part No. 99239) (Part No. DB01-1046 )
Mobile Phase: A: Water B: Acetonitrile Mobile Phase: 0.05M Ammonium Phosphate pH7.0:THF (75:25)
Gradient: Time: 0 15 Flow Rate: 1.0mL/min
%B: 25 60 Detector: UV at 220
Flow Rate: 1.5mL/min
Detector: UV at 254
Anticonvulsants Anticonvulsants
10348 10349
1. Ethotoin 1. Ethotoin
2. Phenytoin 2. Phenytoin
� 3. Mephenytoin � � 3. Mephenytoin
�
� 4. Carbamazepine 4. Carbamazepine
�
� �
� � � � � � � � � � �� �� �� �� �� ������� � � � � � �� �� �� �� �� �������
Column: Alltima™ HP EPS C18, 5µm, 150 x 4.6mm Column: Alltima HP C18 HiLoad, 5µm, 150 x 4.6mm
(Part No. 87715) (Part No. 87697)
Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30) Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30)
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: ELSD Detector: ELSD
Amphetamines
MDA & MDMA Analogs MDA and MDMA Analogs
10014 1 2 4 10427
�
�
1. 2,3-MDA 3 1. 2,3-MDA
2. 3,4-MDA 2. 3,4-MDA
3. 2,3-MDMA 3. 2,3-MDMA
4. 3,4-MDMA 4. 3,4-MDMA
� �
� � � � ������ 0 1 2 3 4 5 6 7 8 9 Min.
Column: Alltima™ Silica, 3µm, 100 x 4.6mm (Part No. 81404) Column: Alltima HP Silica, 3µm, 150 x 4.6mm (Part No. 87786)
Mobile Phase: 1%NH4OH in Methanol:Acetonitrile (25:75) Mobile Phase: 1%NH4OH in Methanol:Acetonitrile(75:25)
Flow Rate: 2.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 280nm Column Temp: 25°C
Detector: UV at 280nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Anti-Hypertensives
Antihypertensives Basic Drugs
9395 10161
3
�
1. Metoprolol 1. Labetalol
�
2. Oxprenolol 2. Acebutolol
1 2 3. Propranolol 3. Oxprenolol
4. Lidocaine
� � �
5. Alprenolol
6. Propranolol
0 1 2 Min. � � � � � �������
Column: Prevail C18, 3µm, 53 x 7mm Part No. (99279)
™
Column: ZirChrom PBD, 3µm, 150 x 4.6mm
®
1. Diltiazem 1. Codeine
2. Verapamil � 2. Strychnine
4 3. Nicardipine � 3. Quinine
3 4. Nifedipine 4. Quinidine
2
�
�
0 1 2 3 4 5 6 7 8 9 10 Min. � � � � � �� �� �� �� �� �� �� �������
Column: Alltima™ HP CN, 5µm, 150 x 4.6mm Column: Alltima™ HP C18 Amide, 5µm, 150 x 4.6mm
(Part No. 87769) (Part No. 87733)
Mobile Phase 0.02M KH2PO4 pH3.0:Acetonitrile (70:30) Mobile Phase: 0.025M Potassium Phosphate pH 2.8:Methanol (90:10)
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 218nm Detector: UV at 254nm
� � � � � ������ � �� �� �������
Column: ZirChrom DiamondBond-C18, 3µm, 100 x 4.6mm Column: Hypercarb®, 7µm, 100 x 4.6mm
(Part No. DB01-1046) (Part No. 59864750)
Mobile Phase: 0.02M Ammonium Phosphate pH11.0: Mobile Phase: Ammonium Acetate pH 9.0:Methanol (40:60)
Tetrahydrofuran (73.5:26.5) Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 220
Detector: UV at 220
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Steroids
1
Steroids Steroids
9392 10155
2
1. Hydrocortisone 1. Progesterone
3 2. Testosterone 2. Corticosterone
3. Progesterone � 3. Cortisone
4. Prednisone
5. Prednisolone
� �
�
�
0 1 Min. � �� �� �������
Column: Prevail™ C18, 3µm, 53 x 7mm Rocket™ Column: Inertsil® Diol, 5µm, 150 x 4.6mm
(Part No. 99279) (Part No. GL-05645)
Mobile Phase: Water:Methanol (10:90) Mobile Phase: Hexane:Ethanol (85:15)
Flow Rate: 4.5mL/min Flow Rate: 1.0mL/min
Detector: UV at 254 Column Temp: 40°C
Detector: UV at 254
Hormones Corticosteroids
10145 10391
� 1. β-Trenbolone 1 1. Deoxycorticosterone
2. α-Trenbolone 2 Acetate
3. β-Zearalenol 5 7 2. Hydrocortisone Acetate
4. Testosterone 3. Cortisone Acetate
5. Zeranol 4. Cortisone
� 6. α-Zearalenol 3 4 6 5. Prednisone
��
�� � 7. α-Estradiol 6. Hydrocortisone
�
8. Zearalenone 7. Prednisolone
�
9. Diethylstilbestrol
0 1 2 3 4 5 6 7 8 9 10 11 12 13 Min.
� �� �������
Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm Column: Alltima™ HP CN, 5µm, 150 x 4.6mm
(Part No. GL-01731) (Part No. 87769)
Mobile Phase: Water:Acetonitrile:THF(57:35:8) Mobile Phase: Hexane:Isopropanol (93.7:6.3)
Flow Rate: 1.0mL/min Flow Rate: 2.0 mL/min
Column Temp: 40°C Column Temp: 35°C
Detector: UV at 230 Detector: UV at 254nm
�
��
� � � � � ������ � � � � � � � � � � �� �� �� �������
Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208)
™
Column: Alltima™ CN, 5µm, 250 x 4.6mm
Mobile Phase: Water:Acetonitrile (10:90) (Part No. 88189)
Flow Rate: 1.0mL/min Mobile Phase: 0.15% HNO3:Acetonitrile (5:95)
Detector: UV at 254 Flow Rate: 0.5mL/min
Detector: ELSD
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
0 1 2 3 4 5 6 7 8 9 10 11 12 Min.
Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056)
Mobile Phase: A: 0.1% TFA in 0.025M Ammonium Acetate B: 0.1% TFA in Acetonitrile
Gradient: Time 0 10 15
%B 25 95 95 3 7
Flow Rate: 1.0 mL/min
Detector: ELSD
0 1 2 3 4 Min.
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ (Part No. 50605)
Mobile Phase: A: 0.1% TFA in 0.025M Ammonium Acetate B: 0.1% TFA in Acetonitrile
Gradient: Time 0 4
%B 20 95
Flow Rate: 3.5mL/min
Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Antibiotics
Antibacterials Antibacterials
8711 8764
3
1. Sulfanilimide 1. Oxytetracycline
2. Cefaclor 1 Dihydrate
� � � 3. Cefatrizine 2 2. Tetracycline HCL
4. Thiamphenicol 3. Meclocycline
� 5. Cefotaxime Sulfosalicylate Salt
�
� � � � � ������� 0 2 4 6 8 10 Min.
Column: Platinum™ EPS C18, 5µm, 150 x 4.6mm Column: Alltima™ C18, 3µm, 100 x 4.6mm (Part No. 81382)
(Part No. 32043) Mobile Phase: A: 0.05M KH2PO4 pH 3.0 B: Acetonitrile
Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile (90:10) Gradient Time: 0 10
Flow Rate: 1.0mL/min %B: 15 40
Detector: UV at 230nm Flow Rate: 1.0mL/min
Detector: UV at 254nm
�
� � �
� � � � � ������ � � � � � � � � � � �� �� �� �� �� �������
Column: Prevail™ Select C18, 5µm, 150 x 4.6mm Column: Alltima™ HP C18 HiLoad, 5µm, 150 x 4.6mm
(Part No. 99300) (Part No. 87697)
Mobile Phase: Water pH9 w/NH4OH:Acetonitrile (5:95) Mobile Phase: 0.1% Phosphoric Acid:Acetonitrile (85:15)
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: ELSD Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Cephalosporins � Tetracyclines
10180 10216
1. Cephalexin 1. Uracil
2. Cephaclor � 2. Minocycline
mAu 1 3. Cephuroxime 3. Tetracycline
�
15 4. Cephoxtin 4. Chlortetracycline
� 5. Doxycycline
10 3
5 2 4 �
0
0 2 4 6 8 10 Min. � � �� �������
Column: ZORBAX® Bonus-RP, 5µm, 150 x 4.6mm Column: ZORBAX® Rx-C8, 5µm, 150 x 4.6mm
(Part No. 883668-901) (Part No. 883967-901)
Mobile Phase: 0.025M Sodium Citrate pH6:Methanol (75:25) Mobile Phase: 0.1% TFA:Acetonitrile (75:25)
Flow Rate: 1.0mL/min Flow Rate: 1.0 mL/min
Detector: UV at 254nm Detector: UV 254nm
Antiseptics
� Antibiotics: High Speed Separation Highly Polar Cosmetic Ingredients
10218 10166
�
1. Ceftazidime � 1. Allantoin
2. Cefotaxime 2. Bronopol
� 3. Ciprofloxacin
4. Ceftazolin
�
�
Column: ZORBAX® SB-C8, 3.5µm, 75 x 4.6 mm Column: ZirChrom® CARB, 3µm, 100 x 4.6mm
(Part No. 866953-906) (Part No. ZR01-1046)
Mobile Phase: 0.1% TFA:Acetonitrile (92:8) Mobile Phase: Water:Acetonitrile (80:20)
Flow Rate: 3.0mL/min Flow Rate: 1.0mL/min
Column Temp.: 60˚C Detector: UV at 200
Detector: UV 260nm
Taxols
Taxol® and Related Taxanes in Yew Taxol® and Related Taxanes in Yew
9221 9241
1. 10-Deacetylbaccatin III 1. 10-Deacetylbaccatin III
4. Taxinine M 4. Taxinine M
2. Baccatin III 2. Baccatin III
5. 7-Xylosyl-10-deacetyltaxol 5. 7-Xylosyl-10-deacetyltaxol
3. 7-Xylosyl-10-deacetyltaxol B 3. 7-Xylosyl-10-deacetyltaxol B
6. 7-Xylosyl-10-deacetyltaxol C 6. 7-Xylosyl-10-deacetyltaxol C
� 7. 10-Deacetyltaxol 7. 10-Deacetyltaxol
8. 7-Xylosyltaxol 11 8. 7-Xylosyltaxol
5 10
9. Cephalomannine (Taxol® B) 1 78 13 9. Cephalomannine (Taxol® B)
� 10. 7-Epi-10-deacetyltaxol 10. 7-Epi-10-deacetyltaxol
9
11. Taxol® 11. Taxol®
� 2
12. Taxol® C 4 6 12 12. Taxol® C
��� � 13. 7-Epitaxol 13. 7-Epitaxol
� � �� �� 3
�
�� ��
� � � � � � � � ������ 0 1 2 3 4 5 6 7 Min.
Column: Alltima™ CN, 3µm, 53 x 7mm Rocket™ (Part No. 81170)
Column: Alltima™ Phenyl 3µm, 53 x 7mm Rocket™ Mobile Phase: A: 1-Methoxy-2-propanol B: Acetonitrile C: Water
(Part No. 81174) Gradient: Time: 0 8
Mobile Phase: 0.05% TFA in Water:Methanol:Acetonitrile %B: 20 65
(50:19:31) %C: 77 32
Flow Rate: 2.5mL/min Flow Rate: 2.5mL/min
Detector: UV at 230nm Detector: UV at 227nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
� �� �� ������� 0 10 20 30 40 50 60 Min.
Column: Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
™ Column: Prevail C18, 5µm, 250 x 4.6mm
™
Ginkgo Biloba
Ginkgo Biloba Flavonol Glycoside Standards Ginkgo Biloba Terpene Lactone Standards
1 4
10239 10287
5
1. Quercetin 1. Bilobalide
2. Kaempferol 2. Ginkgolide J
23
2 3. Isorhamnetin 1 3. Ginkgolide C
4. Ginkgolide A
3 5. Ginkgolide B
0 2 4 6 8 10 12 14 16 18 20 Min. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Platinum C18, 5µm, 250 x 4.6mm Column: Alltima™ C18, 3µm, 100 x 4.6mm
(Part No. 32064) (Part No. 813812)
Mobile Phase: A: 0.05% TFA in Water:Methanol (95:5) Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Methanol
B: 0.05% TFA in Methanol Gradient: Time 0 15
Gradient: Time 0 20 %B 10 55
%B 10 90 Flow Rate: 1.3mL/min
Flow Rate: 1.0mL/min Detector: ELSD
Detector: ELSD
Terpene Lactones High Throughput Liquid Ginkgo Biloba Extract Prep
10288 10315
1. Bilobalide 1. Bilobalide
2. Ginkgolide J 2. Ginkgolide J
3. Ginkgolide C 3. Ginkgolide C
4. Ginkgolide A 4. Ginkgolide A
5. Ginkgolide B � 5. Ginkgolide B
4 5
1 23 � �
� �
0 1 2 3 4 5 6 Min. � � �� �� �� �� �� �� �� �������
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima C18, 5µm, 150 x 10mm
™
Flavonoids
Polyphenolic Flavonoids 1 Flavonoid Mix
9397 2 9271
5
1. Myricetin 1. Myricetin
3
2. Quercetin 2. Quercetin
3. Naringenin 3. Apigenin
1
2 4 4. Hesperetin 4. Kaempferol
6 5. Apigenin 4
6. Kaempferol
3
0 1 2 3 4 5 Min.
0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min.
Column: Prevail™ C18, 5µm, 150 x 4.6mm (Part No. 99208) Column: Alltima™ C18 3µm, 53 x 7mm Rocket™
Mobile Phase: A: 0.025M KH2PO4 pH2.5 B: Acetonitrile (Part No. 50605)
Gradient: Time: 0 15 25 Mobile Phase: 0.1% TFA in Water:0.1% TFA in Acetonitrile
%B: 20 40 40 (65:35)
Flow Rate: 1.0mL/min Flow Rate: 2.5mL/min
Detector: UV at 280 Detector: ELSD
� � �� ������� 0 2 4 6 8 10 12 14 Min.
Column: Alltima™ C18, 3µm, 100 x 4.6mm Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm
(Part No. 81382) (Part No. GL-01731)
Mobile Phase: A: 0.05M KH2PO4 pH 2.5 B: Acetonitrile Mobile Phase: 0.02M NaH2PO4 pH 3.0:Methanol:Tetrahydrofuran
Gradient: Time: 0 15 (50:30:20)
%B: 20 40 Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Column Temp: 40°C
Detector: UV at 280nm Detector: UV at 254
0 1 2 3 Min. 0 1 2 Min.
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™
(Part No. 50605) (Part No. 50605)
Mobile Phase: 0.2% Phosphoric Acid:Methanol (5:95) Mobile Phase: 0.01M K2HPO4 pH2.6:Acetonitrile (80:20)
Flow Rate: 4.0mL/min Flow Rate: 3.5mL/min
Detector: Vis at 585nm Detector: UV at 330nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Green Tea
Green Tea Extract - Analytical Green Tea Extract - Preparative
10139 10140
1. Caffeine 1. Caffeine
2. Epicatechin 2. Epicatechin
3. Epigallocatechin Gallate 3. Epigallocatechin Gallate
�
3
�
� 1 2
� � � � � �� �� �� �� �� ������� 0 2 4 6 8 10 12 14 16 18
Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88052) Column: Alltima™ C18, 5µm, 150 x 22mm (Part No. 81106)
Mobile Phase: 0.05% TFA:Acetonitrile (87:13) Mobile Phase: 0.05% TFA:Acetonitrile (87:13)
Flow Rate: 1.0mL/min Flow Rate: 23mL/min
Detector: ELSD Detector: ELSD
Devil’s Claw
Catechins Iridoid Compounds
8825 9191
�
� 1. Gallic Acid 1. 8-para-Coumaroyl Harpagoside
2. Epigallocatechin 2. Harpagoside
3 Catechin
� � 4. Epicatechin
� 5. Epigallocatechin Gallate 2
� 6. Caffeine
7. Gallocatechin
� � 8. Epicatechin Gallate
1
� � � � � ������ 0 1 2 3 4 5 6 Min.
Column: Platinum EPS C18, 1.5µm, 33 x 7mm Rocket
™ ™
Column: Alltima C18, 3µm, 53 x 7mm Rocket
™ ™
1. Hydrastine � 1. LGG
2. Berberine 2. LGL
3. LLL
4. OGL
5. PGL
6. OLL
� 7. OGO + PLL
��
8. OOO
� �� �
0 1 2 Min. � �� �� �� �������
Column: Alltima™ C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056)
(Part No. 50605) Mobile Phase: A: Acetonitrile B: Dichloromethane
Mobile Phase: 0.02M KH2PO4 pH2.6:Methanol (52:48) Gradient: Time: 0 40
Flow Rate: 3.0mL/min %B: 30 50
Detector: UV at 235nm Flow Rate: 1.0mL/min
Detector: ELSD
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Vitamins
Water- and Fat-Soluble Vitamins Fat-Soluble Vitamins
9545 10279
1. Thiamine 1. Vitamin A (100ng on column)
2. Ascorbic acid 2. Vitamin E (160ng on column)
3. Nicotinic acid 3. Vitamin K1 (130ng on column)
10 4. Niacinamide
56
4 5. Pantothenic acid
�
7 6. Riboflavin
8 7. Cyanocobalamin
8. Biotin �
9 12 14
1 9. Vitamin A
23 11 13 15 �
10. Δ-Tocopherol
11. γ-Tocopherol
12. α-Tocopherol
13. Vitamin D2
0 5 10 15 20 25 30 Min. 14. Vitamin D3 � � � � � � � � � � �������
15. Vitamin K
Column: Prevail™ C18, 5µm, 150 x 4.6mm Column: Alltima™ C18, 3µm, 150 x 1mm
(Part No. 99208) (Part No. 88384)
Mobile Phase: A: Water pH3.2 w/Formic Acid Mobile Phase: Methanol:Chloroform (90:10)
B: Acetonitrile:Methanol (83:17) Flow Rate: 0.05mL/min
Gradient: Time 0 3 10 10.1 30 Detector: ELSD
%B 0 0 45 100 100
Flow Rate: 1.5mL/min
Detector: ELSD
0 2 4 6 8 10 12 14 Min. 0 3 6 9 12 15 Min.
Column: Prevail® C18, 5µm, 150 x 4.6mm Column: Prevail™ C18, 5µm, 150 x 4.6mm
(Part No. 99208) (Part No. 99208)
Mobile Phase: Acetonitrile:Methanol (75:25) Mobile Phase: Acetonitrile:Methanol (75:25)
Flow Rate: 1.5mL/min Flow Rate: 1.5mL/min
Detector: UV at 220 Detector: ELSD
� � � � � �� �� ������� � � � � � �� �� �� �������
Column: Prevail™ C18, 5µm, 150 x 4.6mm Column: Platinum™ C18, 5µm, 150 x 4.6mm (Part No. 32043)
(Part No. 99208) Mobile Phase: A: 0.1M Ammonium Phosphate pH4.5
Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile (97:3) B: Acetonitrile
Flow Rate: 1.0mL/min Gradient: Time: 0 4 15 18
Detector: UV at 212 B%: 5 5 40 40
Flow Rate: 1.0mL/min
Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Carbohydrates
1 Corn Syrup Solids Corn Syrup Oligomers
9354 1 9420
0 5 10 15 20 25 Min. 0 1 2 3 4 5 6 7 8 Min.
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 5µm, 53 x 7mm Rocket™
(Part No. 35101) (Part No. 35104)
Mobile Phase: A: Acetonitrile B: Water Mobile Phase: A: Acetonitrile B: Water
Gradient: Time 0 15 Gradient: Time: 0 4
%B 35 50 %B:, 32 50
Flow Rate: 1.0mL/min Flow Rate: 2.0mL/min
Detector: ELSD Detector: ELSD
0 5 10 15 20 25 30 35 40 Min. 0 2 4 6 8 10 12 14 16 18 Min.
Column: Prevail™ Carbohydrate ES, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 250 x 4.6mm (Part No. 35101)
(Part No. 35101) Mobile Phase: A: Acetonitrile:Acetone (75:25)
Mobile Phase: A: Acetonitrile B: Water B: Water
Gradient: Time: 0 50 Gradient: Time: 0 18
%B: 20 35 %B: 24 50
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: ELSD Detector: ELSD
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
0 2 4 6 8 10 12 Min. � � � � � � � � ������
Column: Prevail™ Carbohydrate ES, 250 x 4.6mm Column: Alltima™ C18, 5µm, 150 x 1mm
(Part No. 35101) (Part No. 88384)
Mobile Phase: Acetonitrile:Water (70:30) Mobile Phase: Methanol:Water (90:10)
Flow Rate: 1.0mL/min Flow Rate: 0.05mL/min
Detector: ELSD Detector: ELSD
0 5 10 15 20 25 30 35 40 45 50 Min. � � �� �� �� �� �� �� �� �������
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm
(Part No. 35101) (Part No. 35101)
Mobile Phase: A: Acetonitrile B:Water Mobile Phase: A: Acetonitrile B: Water
Gradient: Time: 0 50 Gradient: Time: 0 60
%B: 20 35 %B: 17 35
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: ELSD Detector: ELSD
(Part No. 9646) Mobile Phase: Acetonitrile:0.05M Ammonium Acetate pH 5.4, (20:80)
Mobile Phase: 0.005N Sulfuric Acid Flow Rate: 0.3 ml/min
Flow Rate: 0.3mL/min Detector: UV at 230
Column Temp: 60°C
Detector: RI
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Organic Acids
Organic Acid Mix Rye Bread Extract
� 1. Oxalic Acid 9384 9484
�
2. Tartaric Acid
3. Formic Acid �
�� 4. Malic Acid 1. Tartaric Acid
5. Lactic Acid 2. Malic Acid
6. Acetic Acid 3. Lactic Acid
� � 4. Citric Acid
� 7. Diglycollic Acid
� � 5. Fumaric Acid
� 8. Maleic Acid �
�
9. Citric Acid �
�
�� 10. Succinic Acid
�
11. Fumaric Acid
� � � � � � � � ������ � � � � � �������
5
Underivatized Fatty Acids Organic Acid Mix
9539 3 7 8244
0 2 4 6 8 10 12 14 16 18 20 Min. 0 2 4 6 8 10 Min.
Column: Prevail™ Organic Acid, 5µm, 150 x 4.6mm Column: Platinum™ EPS C8, 5µm, 150 x 4.6mm
(Part No. 88640) (Part No. 32420)
Mobile Phase: Acetonitrile:Methanol (75:25) Mobile Phase: 0.025M KH2PO4 pH 2.5:Methanol (97:3)
Flow Rate: 1.0mL/min Flow Rate: 0.7mL/min
Detector: ELSD Detector: UV at 220nm
�
��
Short-Chain Aromatic Fatty Acids Using UV and
�
�
Carboxylic Acids 7067 � ELSD in Tandem 10430
� Triglycerides
�
Long Chain Fatty Acids Olive Oil
9459 � 9515
�
1. Capric Acid � 1. LLO
2. Lauric Acid 2. LLP
3. Myristic Acid 3. OOL
4. Palmitic Acid 4. POL
5. Stearic Acid 5. PPL
� � 6. OOO
� 7. OOP
� 8. PPO
� � �� 9. OOS
�
� � � � ������ � � � � � �� �� �� �������
Column: Prevail® C18, 3µm, 53 x 7mm Rocket™ Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387)
(Part No. 99279) Mobile Phase: A: Dichloromethane B: Acetonitrile
Mobile Phase: Acetonitrile:Methanol (75:25) Gradient: Time: 0 10 18 20
Flow Rate: 3.5mL/min %B: 70 55 70 70
Detector: ELSD Flow Rate: 1.5mL/min
Detector: ELSD
� � � � � �� �� �� ������� � � � � � �� �� �� �������
Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387) Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387)
Mobile Phase: A:Dichloromethane B:Acetonitrile Mobile Phase: A: Dichloromethane B: Acetonitrile
Gradient: Time: 0 10 18 20 Gradient: Time: 0 10 18 20
%B: 70 55 70 70 %B: 70 55 70 70
Flow Rate: 1.5mL/min Flow Rate: 1.5mL/min
Detector: ELSD Detector: ELSD
Additives
Evening Primrose Oil Oxygen Heterocycles in Confectionary Products
1. LGG 1. DHHM
10047 7031
2. LGL 2. Furaneol
� 3. LLL � � 3. Cyclotene
4. OGL 4. HMF
�
5. PGL � 5. HMP
6. OLL � 6. Maltol
7. OGO + PLL
� Reference: Dr. A. Lea and
8. OOO
� Dr. G. Ford, Reading Scientific
�� Services Ltd., Reading, UK.
� �� �
� �� �� �� ������� � � � � � �� �������
Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056) Column: Hypercarb®, 7µm, 100 x 4.6mm (Part No. 59864750)
Mobile Phase: A: Acetonitrile B: Dichloromethane Mobile Phase: A: Perchloric Acid pH1.0
Gradient: Time: 0 40 B: Acetonitrile
%B: 30 50 Gradient: Time: 0 10 15
Flow Rate: 1.0mL/min % B: 1 35 60
Detector: ELSD Flow Rate: 1.0mL/min
Detector: UV at 280nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
� � �� ������� � � � � � �� �� �������
Column: ProSphere C4, 300Å, 5µm, 150 x 4.6mm
™
Column: ProSphere C4, 300Å, 5µm, 150 x 4.6mm
™
� � � � � �� �� �� �� �� ������� � � � � � �� �� �������
Column: ProSphere™ P-HR, 4µm, 150 x 4.6mm Column: ProSphere™ P-HR, 4µm, 100 x 4.6mm (Custom)
(Part No. 35082) Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile
Mobile Phase: A: 0.08% TFA in Water Gradient: Time: 0 15
B: 0.065% TFA in Acetonitrile:Water (95:5) %B: 30 55
Gradient: Time 0 20 Flow Rate: 1.0mL/min
%B 35 85 Detector: UV at 220nm
Flow Rate: 1.0mL/min
Detector: UV at 220nm
Insulins Bradykinins
9082 � 9062
3
1 1. Bovine Insulin 1. des-Pro2-bradykinin
2. Bovine Insulin Impurity 2. Bradykinin
3. Porcine Insulin �
4. Porcine Insulin Impurity
2 4
0 2 4 6 8 10 12 Min. � � � � � �� �� �������
Column: ProSphere C18, 300Å, 5µm, 150 x 4.6mm
™
Column: ProSphere C18, 300Å, 5µm, 150 x 4.6mm
™
2
Bioactive Peptides Peptides
8502 3 4 9066
5
� 1. Methionine Enkephalin 1. GLY-TYR
2. Bradykinin 1 2. VAL-TYR-VAL
� � 3. Leucine Enkephalin 3. Methionine Enkephalin
� �
4. Physalaemin 4. Leucine Enkephalin
5. Substance P 5. Angiotensin II
� � � � � �� �� ������� 0 2 4 6 8 10 12 14 Min.
Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm
(Part No. 32969) (Part No. 32969)
Mobile Phase: A: 0.1% TFA in Water Mobile Phase: A: 0.15% TFA in Water
B: 0.085% TFA in Acetonitrile:Water (95:5) B: 0.13% TFA in Acetonitrile
Gradient: Time 0 20 Gradient: Time: 0 15
%B 20 50 %B: 10 30
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 220nm Detector: UV at 220nm
� � � � � �� �� �� ������� � � �� �� �� �������
Column: ProSphere™ C18, 100Å, 5µm, 250 x 4.6mm Column: ProSphere™ P-HR, 4µm, 150 x 4.6mm
(Part No. 35087) (Part No. 35082)
Mobile Phase: A: 0.1% TFA in Water Mobile Phase: A: 0.08% TFA in Water B: 0.065% TFA in
B: 0.1% TFA in Acetonitrile:Water (90:10) Acetonitrile:Water (95:5)
Gradient: Time 0 20 Gradient: Time 0 1 30
%B 20 50 %B 14 14 28
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 220nm Detector: UV at 220nm
� � � � � �� �� �� �� �� ������� 0 5 10 15 20 25 Min.
Column: ProSphere™ C18, 300Å, 5µm, 150 x 4.6mm Column: ZORBAX® 300 Extend-C18, 3.5µm, 150 x 2.1mm
(Part No. 32969) (Part No. 763750-902)
Mobile Phase: A: 0.1% TFA in Water Flow Rate: 0.25mL/min
B: 0.08% TFA in Acetonitrile Detector: UV at 210nm
Gradient: Time: 0 20
%B: 20 40
Flow Rate: 1.0mL/min
Detector: UV at 220nm
� � � � � �� �� �� ������� 0 5 10 15 20 25 Min.
Column: Alltima HP C18, 190Å, 5µm, 250 x 4.6mm (Part No. 87679) Column:
™
ZORBAX 300 Extend-C18, 3.5µm, 150 x 2.1mm
®
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile (Part No. 763750-902)
Gradient: Time 0 20 Flow Rate: 0.25mL/min
%B 20 40 Detector: UV at 210nm
Flow Rate: 1.0mL/min
Detector: UV at 220nm
Aβ(1-38)
� � � � � �� �� �� ������� 0 5 10 15 20 25 Min.
Column: ProSphere C18, 100Å, 5µm, 250 x 4.6mm
™
Column: ZORBAX® 300 Extend-C18, 3.5µm, 150 x 2.1mm
(Part No. 35091) (Part No. 763750-902)
Mobile Phase: A: 0.1% TFA in Water B: 0.08% TFA in Acetonitrile Flow Rate: 0.25mL/min
Gradient: Time 0 20 Detector: UV at 210nm
%B 20 40
Flow Rate: 1.0mL/min
Detector: UV at 220nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
� 1. Myoglobin � 1. Myoglobin
� �
2. Conalbumin 2. Ribonuclease A
3. Bovine Serum Albumin 3. α-Chymotrypsinogen
4. β-lactoglobin A � 4. Cytochrome C
5. Lysozyme
� � � � � �� �� �� ������� � � �� �� �� �� �������
Column: ProSphere P-WAX, 10µm, 75 x 7.5mm
™
Column: ProSphere P-WCX, 10µm, 75 x 7.5mm (Part No. P20351)
™
� � � � � �� �� �� ������� � � �� �� �� �� �������
Column: ProSphere™ P-SAX, 10µm, 75 x 7.5mm Column: ProSphere™ P-SCX, 10µm, 75 x 7.5mm (Part No. P20347)
(Part No. P20336) Mobile Phase: A: 0.02M Potassium Phosphate pH 7
Mobile Phase: A: 0.02M Tris pH8 B: 0.02M Potassium Phosphate,
B: 0.02M Tris, 1.0M Sodium Acetate pH8 0.5M Sodium Chloride pH 7
Gradient: Time 0 1 20 25 Gradient: Time 0 30 35
%B 0 28 30 0 %B 0 75 0
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: UV at 280nm Detector: UV at 280nm
Strong Anion Exchange of Egg White High Speed Human Hemoglobin Analysis
10401 10397
� � � � � �� �������
Column: ProSphere P-SAX-HR, 7µm, 50 x 4.6mm
™
� � � � � �� �� �� �� �� �� �� �� �� �� ������� 0 15 30 45 Min.
Column: ProSphere™ SEC 125, 5µm, 300 x 7.8mm Column: ProSphere™ P-HIC Ether, 10µm, 75 x 7.5mm
(Part No. P20325) (Part No. P20316)
Mobile Phase: 0.3M Sodium Chloride, Mobile Phase: A: 1.8M Ammonium Sulfate, 0.1M Potassium Phosphate
0.05M Potassium Phosphate pH 7.0 pH 7.0 B: 0.1M Potassium Phosphate pH 7.0
Flow Rate: 1.0mL/min Gradient: Time 0 60
Detector: UV at 254nm %B 0 100
Flow Rate: 1.0mL/min
Detector: UV at 280nm
Proteins (<120,000 DA) by SEC �
Proteins by HIC
10372 � 10389
�
� 1. Thyroglobulin � 1. Myoglobin
2. IgG 2. Ribonuclease A
3. Phosphorylase b � 3. Lysozyme
4. Ovalbumin � 4. α-Chymotrypsin
�
� � �
5. Ribonuclease A 5. α-Chymotrypsinogen
6. GLY-TYR
� � � � � �� �� �� �� �� �� �� �� �� �� ������� � �� �������
Column: ProSphere SEC 250, 5µm, 300 x 7.8mm
™
Column: ProSphere P-HIC Phenyl, 10µm, 75 x 7.5mm
™
1. Myoglobin
1
2. Ribonuclease A
2 4 3. Lysozyme
4. a-Chymotrypsin
5. a-Chymotrypsinogen
0 2 4 6 8 10 Min.
Column: ProSphere P-HIC NP-Butyl, 2.5µm, 33 x 4.6mm
™
� � �� �� �� �������
Column: ProSphere™ P-SCX-HR, 7µm, 50 x 4.6mm � �� �������
(Part No. P20344) Column: ProSphere™ ABA, 10µm, 75 x 7.5mm
Mobile Phase: A: 0.02M Sodium Acetate pH 5.0 (Part No. P20353)
B: 1M NaCl, 0.02M Sodium Acetate pH 5.0 Mobile Phase: A: 0.5M NaCl, 2mM CaCl2, 0.05M Tris-HCl pH 8.0
Gradient: Time: 0 20 B: 0.5M NaCl, 2mM CaCl2, 0.05M Glycine-HCl pH 2.8
%B 0 100 Gradient: Time 0 10 10.1 30
Flow Rate: 1.0mL/min %B 0 0 100 100
Detector: UV at 280nm Flow Rate: 1.0mL/min
Detector: UV at 280nm
Amino Acids
Amino Acids without Derivatization Thyroid Chemicals
1. Gly 5. Ala/Thr 9. Pro 1. DL-Tyrosine
9413 8282
5 2. Ser/Asn 6. Glu 10. Arg 2. 3-Iodo-Tyrosine
4 3. Asp 7. Cys/Lys 11. Val 3. 3,5- Diiodo-D-Tyrosine
3 4. Gln 8. His 12. Met 4. DL-Thyroxine
11 13. Tyr 2 5. 3,3,5-Triiodo-L-Thyronine
2 7 13 14. Ile 1 3
1 12 15. Leu
14 5
16 16. Phe 4
17 17. Trp
6 15
8 9
10
0 5 10 15 20 25 Min. 0 2 4 6 8 10 12 Min.
Column: Prevail™ C18, 5µm, 250 x 4.6mm (Part No. 99210) Column: Platinum™ EPS C18, 5µm, 150 x 4.6mm
Mobile Phase: A: 5mM Heptafluorobutyric Acid, 0.7% TFA (Part No. 32214)
B: Acetonitrile Mobile Phase: 0.05M KH2PO4, pH 3.2:Acetonitrile (65:35)
Gradient: Time: 0 6 8 25 Flow Rate: 1.0mL/min
%B: 0 0 15 35 Detector: UV at 254nm
Flow Rate: 1.0mL/min
Detector: ELSD
Catechols
Phenylalanine Catecholamines
8770 10148
1. VMA
2. 5-HIAA
O 3. HVA
�
OH �
NH2 �
4 6 8 10 12 Min. � �� �������
Column: Chirobiotic T , 250 x 4.6mm (Part No. 12053)
™
Column: Inertsil CN-3, 5µm, 150 x 4.6mm
®
Nucleic Acids
Nucleosides Nucleosides
� 10347 8245
� � 1. Cytidine 1. Cytidine
2. Uridine 2. Uridine �
3. Xanthosine 3. Xanthosine �
� �
4. Guanosine 4. Guanosine �
5. Adenosine 5. Adenosine �
� � � � � �� ������� � � �� �� �������
Column: Alltima HP EPS C18, 5µm, 150 x 4.6mm
™
Column: Platinum EPS C18, 5µm, 250 x 4.6mm
™
� 1. Cytidine 1. Cytosine
2. Uridine 2. Uracil
� 3. Xanthosine 3. Guanine
� � �
4. Guanosine � 4. Adenine
5. Adenosine � 5. Thymine
�
�
� � � � ������ � � � � ������
Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208)
®
Column: Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208)
™
Mobile Phase: 0.025M KH2PO4 pH 3.0:Acetonitrile:(96:4) Mobile Phase: 0.025M KH2PO4 pH3.0:Acetonitrile:(98:2)
Flow Rate: 1mL/min Flow Rate: 1.0mL/min
Detector: UV at 254 Detector: UV at 254
���
� �� �� ������� � � � � � �� �� �������
Column: ProSphere™ Boronate, 10µm, 75 x 7.5mm Column: ProSphere™ P-WAX-NP, 2.5µm, 35 x 4.6mm (Part No. P20339)
(Part No. P20354) Mobile Phase: A: 0.02M Tris-HCl pH 9.0
Mobile Phase: 0.1M Phosphate, pH 8.0 B: 1M NaCl, 0.02M Tris-HCl pH 9.0
Flow Rate: 1.0mL/min Gradient: Time 0 15
Detector: UV at 280nm %B 48 65
Flow Rate: 1.5mL/min
Detector: UV at 260nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Miscellaneous
Total E.coli RNA by Anion Exchange Phospholipids
10402 1. 1,2-Phosphatidyl-ethanolamine 10314
� 2. Phosphatidyl-DL-glycerol
�������� 3. Phosphatidylinositol
�������
4. 1,2-Phosphatidyl-L-serine
����������
5. Phosphatidylcholine
�������� 6. Sphingomyelin
� �
������� �
������� � �
� �� ������� � � � � �� �� �� �� �������
Column: ProSphere™ P-WAX, 10µm, 75 x 7.5mm (2) Column: Prevail™ Silica, 3µm, 150 x 4.6mm (Part No. 99275)
(Part No. P20341) Mobile Phase: A: Isopropanol B: Hexane C: Water
Mobile Phase: A: 0.3M NaCl, 0.1M Tris-HCl pH 7.6 Gradient: Time 0 7 15
B: 1.0M NaCl, 0.1M Tris-HCl pH 7.6 %B 40 40 40
Gradient: Time 0 90 %C 2 8 8
%B 0 30 Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Detector: ELSD
Detector: UV at 260nm
2 3
1. 3-Amino-4-hydroxybenzoic Acid 1. DL-4-Hydroxy-3-methoxy-mandelic Acid
2. 4-Hydroxybenzoic Acid 2. Homovanillic Acid
3. Benzoic Acid 3. Vanillic Acid
4. 4-Phenylbutyric Acid 4. Salicylic Acid
1 5. 5-Phenylbutyric Acid 5. Benzoic Acid
2
4 5
4 5
0 2 4 6 8 10 12 14 Min. 0 1 2 3 4 5 6 7 8 9 10 11 Min.
Column: Prevail Organic Acid, 5µm, 150 x 4.6mm
™
Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88052)
(Part No. 88640) Mobile Phase: 0.05M KH2PO4 pH 3.2:Methanol (60:40)
Mobile Phase: 0.025M KH2PO4 pH2.5:Acetonitrile (60:40) Flow Rate: 1.0mL/min
Flow Rate: 1.0mL/min Detector: UV at 254nm
Detector: UV at 254nm
Monoaromatics Alcohols
2
7 8982 � 5440
9 10
1. Uracil � 1. Glycerol
2. Benzamide � 2. Methanol
3. Benzyl Alcohol � 3. Ethanol
3 � ��
4 4. Phenol � 4. iso-Propanol
5. Methylbenzoate � � � 5. tert-Butanol
6. Acetophenone �� 6. Propanol
6 7. N,N-diethyl-m-toluamide 7. sec-Butanol
5 ��
8. Toluene 8. iso-Butanol
1 9. Benzophenone 9. tert-Pentanol
8
10. Biphenyl 10. Butanol
11. iso-Pentanol
12. Pentanol
0 1 2 3 4 Min. � � � �� �� �� �� �������
Column: Platinum™ EPS C18, 3µm, 53 x 7mm Rocket™ Column: OA-1000, 9µm, 300 x 6.5mm (Part No. 9046)
(Part No. 50573) Mobile Phase: 0.01N Sulfuric Acid
Mobile Phase: 0.05M KH2PO4 pH 3:Methanol (50:50) Flow Rate: 0.8mL/min
Flow Rate: 2.3mL/min Column Temp: 65°C
Detector: UV at 254nm Detector: RI
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Pesticides
Organophosphates Organochlorines
10346 8437
�
1. Methyl parathion 1. Methoxychlor
2. Dichlorvos 2. Dieldrin
1 3 3. Ethoprophos 3. Heptachlor
4. Azinphos 4. 4,4-DDT
�
2 4
�
�
0 4 8 12 16 20 24 Min. � � � � �������
Column: Alltima HP EPS C18, 5µm, 150 x 4.6mm
™
Column: Platinum C18, 5µm, 150 x 4.6mm (Part No. 32043)
™
2
1. Simazine 3 1. Fenuron
2. Simetryn 2. Monuron
� 3. Prometon 3. Diuron
4
4. Atrazine 4. Linuron
� 5. Ametryn
�
6. Prometryn
� � 7. Terbutryn
�
� 1
� � � � � ������ 0 1 2 3 4 5 6 7 8 Min.
Column: Platinum™ EPS C18, 1.5µm, 33 x 7mm Rocket™ Column: Alltima HP CN, 5µm, 150 x 4.6mm
™
2
� 1. Asulam 1. Diquat
2. MCPP 2. Paraquat
3. Oxine-copper
4. Thiuram
5. Iprodione
6. Bensulide
7. Pencycuron
� �
�
�
0 2 4 6 8 Min.
� � � � � �� �� �� ������
Column: Platinum™ Silica 100Å 3µm, 30mm x 4.6mm (Custom)
Column: Inertsil® ODS-3, 5µm, 150 x 4.6mm Mobile Phase: 0.01M Tetramethylammonium Hydroxide and
(Part No. GL-01731) 0.15M Ammonium Sulfate in Water
Mobile Phase: 0.05M KH2PO4 pH3:Acetonitrile (40:60) pH 3.0 with 5M Sulfuric Acid
Flow Rate: 1.0mL/min Flow Rate: 0.5mL/min
Column Temp: 40°C Detector: UV at 310nm until 3 minutes
Detector: UV at 230nm UV at 260nm after 3 minutes
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
PAHs
Polyaromatic Hydrocarbons
9400
Phthalates Phenols
Phthalic Acid Dialkyl Esters Endocrine Disruptors
10149 10150
1. diethylester 1. Phenol
2. di-isopropylester 2. Diethylphthalate
3. di-n-butylester 3. Bisphenol-A
2 4
4. di-isobutylester 4. 2,4-Dichlorophenol
� 5. dicyclohexylester 5. 4-Octylphenol
6. di-n-heptylester 6. 4-Nonylphenol
5
���� 7. di-2-ethylhexylester
� 1
8. di-n-decylester 3
9. Phthalic Acid di-n-dodecylester
�� �� �� 6
� �
� �� ������� 0 2 4 6 8 10 12 14
Column: Inertsil® C8-3, 5µm, 150 x 4.6mm Column: Inertsil® CN-3, 5µm, 250 x 4.6mm
(Part No. GL-01900) (Part No. GL-01941)
Mobile Phase: A: Water B: Acetonitrile Mobile Phase: 0.2M KH2PO4 pH 3.0:Acetonitrile (55:45)
Gradient: Time: 0 5 10 Flow Rate: 1.0mL/min
%B: 75 75 100 Column Temp: 40°C
Flow Rate: 1.0mL/min Detector: UV at 280
Column Temp: 40°C
Detector: UV at 254
Nitroaromatics Explosives
Nitroaromatics (Normal phase) Nitroaromatic and Nitroamine Explosives
3 1. Nitrobenzene 10358 8279
1. HMX 7. TNT
2. 2,6-Dinitrotoluene 2. RDX 8. 4-A-DNT
� �
3. 1,3-Dinitrobenzene 3. TNB 9. 2,6DNT
4. 4-Nitrophenol 4. DNB 10. 2,4DNT
2 5. Tetryl 11. 2-NT
6. NB 12. 3-NT
� 13 4-NT
� � � ��
�
4 �� �� ��
� �
1
0 1 2 3 4 5 6 7 8 Min.
� � � � � �� �� �� �� �� �� �������
Column: Alltima HP CN, 5µm, 150 x 4.6mm ™
Column: Platinum™ C18, 5µm, 250 x 4.6mm (Part No. 32064)
(Part No. 87769) Mobile Phase: Water:Methanol (50:50)
Mobile Phase: Hexane:Isopropanol (85:15) Flow Rate: 0.7mL/min
Flow Rate: 1.0mL/min Column Temp: 30°C
Detector: UV at 254nm Detector: UV at 254nm
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Aflatoxins
Aflatoxins Aflatoxins
10144 10437
� �
� 1. Aflatoxin G2 1. Aflatoxin B1
2. Aflatoxin G1 2. Aflatoxin G1
3. Aflatoxin B2 3. Aflatoxin B2
4. Aflatoxin B1 � 4. Aflatoxin G2
� � �
� � �� �������
� � � � � �������
Column: Inertsil® ODS-3V, 5µm, 150 x 4.6mm Column: Alltima™ HP Silica, 5µm, 150 x 4.6mm
(Part No. GL-01801) (Part No. 87795)
Mobile Phase: Water:Methanol (50:50) Mobile Phase: Methylene Chloride:Methanol:Water (99.35:0.5:0.15)
Flow Rate: 1.0mL/min Flow Rate: 0.8mL/min
Column Temp: 40°C Detector: ELSD
Detector: UV at 365
� � � � � �� �� �� �� �� �� �������
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Alltima HP C18 HiLoad, 3µm, 100 x 4.6mm
™
Column: Alltima C18, 5µm, 250 x 4.6mm
™
Polymers
Polyurethane Curatives 7472 Polystyrene 7213
� 1. 8.42M
2. 1.3M
� 3. 791K
1. Polytetramethylene Glycol 4. 354K
2. Ethecure 300 � 5. 98.9K
� 6. 43K
� � ��
� � � 7. 10.1K
�
� 8. 2.6K
9. 530K
10. 120K
Column: Jordi DVB, 500Å, 500mm x 10mm (6) Column: Jordi DVB, Mixed Bed Linear, 500mm x 10mm
(Part No. 100567) (Part No. 100583)
Mobile Phase: Chloroform Mobile Phase: THF
Flow Rate: 1.5mL/min Flow Rate: 1.5mL/min
Column Temp: 50°C Column Temp: 40°C
Detector: RI Detector: RI
Tween 80 Dimethicone
7809 8636
� � �� �� �� �� �� � � � �� �� �������
Column: Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072)
™
Column: Alltima™ C18, 5µm, 250 x 4.6mm Mobile Phase: A: Acetonitrile B: Chloroform
(Part No. 88056) Gradient: Time: 0 20
Mobile Phase: Water:Methanol (40:60) %B: 25 75
Flow Rate: 1.0mL/min Flow Rate: 1.0mL/min
Detector: ELSD Detector: ELSD
© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.
Chelators
Phenone Homologs Metal Chelators
9460
� 8584
1 4 5
7
3 1. Uracil 1. 2,2’-Dipyridyl
2. Theophylline 2. 8-Hydroxyquinoline
3. Acetophenone �
6 8 4. Propiophenone
9 5. Butyrophenone
6. Benzophenone
2 7. Valerophenone
8. Heptanophenone
9. Octanophenone
0 1 2 3 Min.