Gram Negative Rods Stool Pathogens Flowchart

Gram negative rods

(GNR) from stool

Lac (-) on Lac (+) on

MAC MAC

Oxidase (-)* Oxidase (+)* See GNR Stool

Pathogens
Lactose Positive
Flowchart
Possible Salmonella, Shigella, Growth
Yersinia enterocolitica, pathogenic on TCBS
E. coli or Proteus spp.

Yellow Green None

Swarming on
BAP

Possible Vibrio Yellow colonies

Possible Vibrio
parahemolyticus on HE
No Yes cholerae (left),
V. alginolyticus (right)

Oxidase (+)*
Proteus
Indole*
spp.
Refer to GNR Stool
Pathogens Lactose Possible
Negative Flowchart Aeromonas
(+)* (-)*

Potential pathogenic E. coli Refer to GNR Stool

see GNR Stool Pathogens
Lactose Positive Flowchart
and follow ID for E. coli
Pathogens Lactose
Negative Flowchart
* = See Biochemical Tests for Gram
Negative Organism ID Job aid for positive
and negative result reference.

Laboratory Training Branch