Title: “Determination of vitamin C content (Titration method with 2,6

dichlorophenolindophenol-2,6-DCIP)
Method
1. Preparation:
First, determine the concentration correction factor (f) of the 2,6 DCTP solution. A 5ml aliquot
of pure vitamin, which had been diluted in 2% H2SO4, and 2.5 ml of a solution of satutamin
Oxalatamon were transferred into a 50 ml triangular flask (designated as flask 1). Afterward, the
above mixture was titrated with a 2.6 DCIP solution (analytical) until a stable pink color
persisted for 1 minute and recorded as a1ml. A 5 ml aliquot of pure vitamin, which had been
diluted in a 2% H2SO4 solution, was transferred into a 50 ml triangular flask (designated as flask
2) using a micropipette. A few crystals of KI and 5 drops of starch indicator solution were added
to flask 2. Flask 2 was titrated with 0,001N KIO3 solution until the solution turned blue and
recorded the volume as b1ml. The coefficient f of the 2,6 DCIP solution used in the analysis was
calculated according to the formula: f=b1/a1.
The mass of tangerines was accurately weighed to 10g and then placed in a mortar, where they
were ground in 5% metaphosphoric acid (or 1% HCl). Subsequently, the mixture was thoroughly
shaken, filtered, and the resulting vitamin C filtrate was collected for subsequent analysis.
2. Reaction procedure
Experimental sample:
A micropipette was used to transfer 10ml of the filtered analytical vitamin C solution and 5ml of
saturated oxalate monohydrate into a clean, dry 50ml triangular flask (designated as flask 3). The
flask 3 was titrated with 2,6 DCIP solution (analytical) until a stable pink color persisted for 1
minute.
Control sample:
A pipette was used to transfer 10ml of 1% HCl solution and 5ml of saturated oxalate
monohydrate into a clean, dry 50ml triangular flask (designated as flask 4). The flask 4 was
titrated with 2,6 DCIP solution (analytical) until a stable pink color persisted for 1 minute.