Hitachi L-2100

INSTRUCTION MANUAL
FOR
MODEL L-2100 PUMP
Copyright  Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan. Part No. 890-9101
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PREFACE
Thank you very much for purchasing Hitachi Model L-2100/2130
pump, which has been specifically designed for automatic
analysis in a liquid chromatograph system.
The Model L-2100/2130 pump is intended for use by persons
having a basic knowledge of chemical analysis.
Remember that improper use of analytical instruments,
chemicals or samples would result not only in wrong analytical
data but also in consequences adverse to safety.
Carefully read this instruction manual before attempting
operation. For proper use of the pump, please acquaint yourself
with it.
ABOUT THIS MANUAL

The operating procedures and maintenance/checkup instructions
for the L-2100/2130 pump are described in this manual.
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the
beginning of this manual for ensuring safety in operation of the
pump.
If installation of the pump is not yet completed, refer to Section 2.
Before attempting operation, read through Sections 3 and 4 to
become acquainted with the structure of the instrument and its
basic operations.
-1-

-2-

IMPORTANT
Precautions on Electromagnetic Wave Interference
1. Possible Electromagnetic Wave Interference Caused by This Instrument
Where this instrument is used in a residential area or an

adjacent area thereto, it may cause interference to radio and
television reception.
To prevent this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference caused by it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not be caused by the instrument.
If the instrument does cause interference to radio or television
reception, which can be determined by turning the instrument off
and on, the user is encouraged to try to correct the interference
by one or more of the following measures:
• Reorient the antenna of the radio/television receiver.
• Increase separation between the instrument and the radio/TV
receiver.
• Connect the instrument to an outlet on a circuit different from
that to which the radio/TV receiver is connected.
2. Possible Electromagnetic Wave Interference Affecting This Instrument
Where this instrument is used near an intense electromagnetic

source, interference noise may be given to the instrument to
incur an adverse effect on its performance or functionality.
To present this, use the specified system connection cables in
strict accordance with the instruction manual. The instrument is
designed to minimize possible electromagnetic wave
interference affecting it if the specified cables are connected
properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in the instrument. If the instrument
does incur electromagnetic wave interference, which can be
determined by turning on and off possible sources of
electromagnetic interference nearby, the user is encouraged to
try to correct the interference by one or more of the following
measures:
-3-

• Reorient the instrument.
• Increase separation between the instrument and possible
sources of electromagnetic interference.
• Connect the instrument to an outlet on a circuit different from
that to which possible sources of electromagnetic interference
are connected.
• Check that any other device connected with the instrument is
not affected by electromagnetic interference.
Warranty on Product
The Model L-2100 pump is warranted to be free from defects in

material or workmanship under normal use within the product
specifications indicated in this manual and under conditions
given below. This warranty is void if the instrument is not used
according to the instruction manual.
(1) Scope of Warranty
Any parts which prove to be defective in design or

workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part may
be used for repair, or replacement with an equivalent
product may be made instead of repair. Such system
components as a personal computer and printer to be
updated frequently for improvement may not be available in
original versions at the time of replacement. Note that this
warranty does not apply to the instrument if modified by the
user or resold without permission from the manufacturer,
consumable parts, and any failure of lifetime-expired parts.
(2) Warranty Period
One year from the date of initial installation
(3) Availability of Technical Support Service
Technical support service for this instrument is available

within regular working hours on workdays specified by
Hitachi High-Technologies Corporation.
-4-

(4) Limitations and Exclusions on Warranty
Note that the following cases are excluded from the scope
of this warranty.
(a) Failure due to operation at a place not meeting the

installation requirements specified by Hitachi High-
Technologies Corporation.
(b) Failure due to power supply voltage/frequency other
than specified by Hitachi High-Technologies
Corporation or due to abnormality in power supply.
(c) Corrosion or deterioration of the tubing due to
impurities contained in reagent, gas, air or cooling
water supplied by the user.
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric
gas.
(e) Failure due to use of hardware, software or spare parts
other than specified by Hitachi High-Technologies
Corporation.
(f) Failure due to improper handling or maintenance by
the user.
(g) Failure due to maintenance or repair by a service
agent not approved or authorized by Hitachi High-
Technologies Corporation.
(h) After disposal of this instrument, or after its resale
without Hitachi High-Technologies Corporation
approval.
(i) Failure due to relocation or transport after initial
installation.
(j) Failure due to disassembly, modification or relocation
not approved by Hitachi High-Technologies
Corporation.
(k) Consumables, and failure of parts that have reached
the end of specified useful life.
(l) Failure of parts excluded from the warranty in the
instruction manual or other documents.
(m) Failure due to acts of God, including fire, earthquake,
storm, flood, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.
(n) Failure of the hardware, or damage to the system
software, application software, data or hard disk due to
computer virus infection.
-5-

(o) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to power
interruption or momentary power voltage drop caused
by lightning.
(p) Failure of the personal computer connected with the
instrument, or damage to the system software,
application software, data or hard disk due to
disconnection of main power to the personal computer
without taking the specified normal shutdown
procedure.
(5) Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES,

EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED
HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE
OR USE, OR AGAINST INFRINGEMENT OF ANY
PATENT. IN NO EVENT SHALL THE MANUFACTURER
BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR
LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE
THE SCOPE OF THIS WARRANTY.
(6) Warranty Card
The warranty card will not be reissued. Keep it in a safe

place.
Installation, Relocation and After-sale Technical Service
Installation of the instrument shall be carried out by or under

supervision of qualified service personnel of the manufacturer or
its authorized service agent.
Before installation of the instrument, the customer is requested
to prepare necessary utilities referring to this manual.
When relocation of the instrument becomes necessary after
initial installation (delivery), please notify your local Hitachi High-
Technologies sales representative or service office.
-6-

Technical Seminars and Training for Customers
For the customers to acquire in-depth understanding of the

analytical instruments, technical seminars and customer training
courses are available at Hitachi High-Technologies Corporation
or your site. For further information, contact your local Hitachi
High-Technologies Corporation sales representative.
(The technical seminars and customer training courses are
available on a chargeable basis.)
Other Precautions
1. Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal

standards and regulations in the handling, storage and
discarding of chemicals and samples used in analytical
operations of the instrument.
(2) Reagents, standard solutions and accuracy-control

samples shall be handled, stored and discarded as
instructed by the respective suppliers.
2. Notice on This Instruction Manual
(1) The information contained in this manual is subject to

change without notice for product improvement.
(2) This manual is copyrighted by Hitachi High-Technologies

Corporation with all rights reserved.
(3) No part of this manual may be reproduced or transmitted in

any form or by any means without the express written
permission of Hitachi High-Technologies Corporation.
-7-

1.1
1. OUTLINE
The liquid chromatograph system consists of a pump for feeding

an eluent, a sample introducing section (autosampler, manual
injector) for injecting a sample, a column for chromatographic
separation of the injected sample, a column oven for maintaining
the column at a constant temperature, and a detector section for
detecting a signal of a separated component of the sample.
In addition, a degasser for deaerating an eluent, a gradient
device for varying a mixing ratio of eluents with time, and other
optional devices are incorporated in the system.
1.1 Isocratic System
The isocratic system has a simple configuration in which single-

solvent analysis is performed.
Washing solution Eluent
System power supply unit
Model L-2400 UV detector
Model L-2300 column oven

Column
Model L-2200 autosampler
Model L-2100/2130 pump

Degasser
Drain
1-1

1.1 Isocratic System
(1) An eluent is fed by the pump through the degasser.
(2) A sample is injected from the autosampler.
(3) The injected sample is separated through the column which

is maintained at a constant temperature by the column
oven.
(4) Each component separated from the sample is then
detected as a signal by the detector.
1-2

1.2
1.2 Low-Pressure Gradient System
In the low-pressure gradient system, two or more solvents are

mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
Washing solution Eluent

Column
Model L-2200 autosampler
Low-pressure
gradient unit
Mixer
Degasser
Drain
(1) Each of plural eluents is fed from the degasser to the low-
pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-

specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) A sample is injected by the autosampler.

oven.
(6) Each component separated from the sample is detected as

a signal by the detector.
1-3

1.3 High-Pressure Gradient System
1.3 High-Pressure Gradient System
In the high-pressure gradient system, two or more solvents are

fed by respective pumps and mixed in the high-pressure section
for liquid delivery.
Eluent
Washing Model L-2400
solution UV detector
System power
Model L-2300
supply unit
column oven
Column
Model L-2100/
2130 pump
Mixer
Model L-2100/ Model L-2200
2130 pump autosampler
Degasser
Drain
(1) From the degasser, each of plural eluents is applied to the

pump.
(2) The plural eluents are fed at a flow rate corresponding to a

pre-specified mixing ratio.
(3) The plural eluents thus fed are mixed in the high-pressure
gradient section. Through the mixer, the mixture eluent is
delivered.
(4) A sample is injected by the autosampler.

oven.
(6) Each component separated from the sample is detected as

a signal by the detector.
1-4

2.1
2. INSTALLATION
CAUTION
Beware of heavy objects!
This instrument is as heavy as 15 kg.

When carrying this instrument, exercise care not to incur injury
by dropping it off accidentally. Be sure to hold the front and
rear parts of the instrument securely when moving it.
2.1 Unpacking
Carefully unpack the shipping case, take out module units and
standard accessories, and place them on a sturdy, level desk or
bench.
NOTICE: Installation of this instrument shall be carried out by

or under supervision of qualified service personnel
of Hitachi High-Technologies Corporation or its
authorized service agent.
Before installation of this instrument, the customer
shall make preparations for satisfying the
installation requirements in accordance with this
manual.
When relocation of this instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.
2-1

2.2 Installation Place
2.2 Installation Place
When selecting an installation place for this instrument, be sure

to check the following conditions:
(1) Operating Ambient Temperature
4 to 35 °C
Temperature variations during measurement should be
minimized. Take sufficient care to prevent dew
condensation.
(2) Operating Ambient Humidity
45 to 85% RH
(3) Atmosphere
(a) Well ventilated.
(b) Free from acidic or alkaline gases that could corrode

metals significantly.
(c) Free from vapors of solvents (e.g., benzene, thinner)

that could affect the paint coating on the instrument.
(4) Other Requirements
(a) Not exposed to direct sunlight.

Exposure to direct sunlight could result in deteriorated
performance or discoloration of the instrument.
Avoid installing the instrument near a window of the
laboratory room.
(b) Not exposed to drafts directly.
(c) Free from intense vibrations and shocks.
(d) Apart from a heat source such as a gas burner,

electric heater, or oven.
(e) Apart from a strong magnetism source such as an

electronic welder, high-frequency electronic furnace, or
voltage transformer.
2-2

2.2
(f) Free from an amount of dust and dirt.

Contamination with dust and dirt could deteriorate the
performance of the instrument.
(g) No abrupt fluctuations in power voltage.

Fluctuations in power voltage could cause noise in
analytical data.
(h) Do not connect the instrument to a power line used by

an electric device that is not equipped with a noise
suppressor circuit (e.g., stirrer, vibrator).
2-3

2.3 Items to be Prepared by the User
2.3 Items to be Prepared by the User
2.3.1 Power Supply (1) Power voltage : 100 to 240 V AC

Fluctuation .......Within ±10% with respect
to rated voltage
(2) Frequency : 50 or 60 Hz
Fluctuation .......Within ±0.5 Hz with respect
to rated frequency
(3) Power
consumption : 500 VA or more in total system
Provide power supply sufficient for
combinational use with other devices.
(4) Grounding : Grounding connection of Electric

Installation Technical Standard Class 3 or
higher should be provided.
WARNING
Ground Properly to Prevent Electric Shock Hazard!
• Be sure to use the power cable supplied with the

instrument. Use of a different power cable could result in
an electric shock hazard.
• This instrument is of a "plug-connected type" specified in
European Norm EN61010-1, so connect the power cable to
a grounded three-wire outlet.
• If a grounded three-wire outlet is not available, the power
cable may be connected to a two-wire outlet through an
adapter plug. In this case, be sure to provide proper
grounding connection as required.
2-4

2.3.2
Power outlet
Table tap with
ground cable
Ground resistance: Ground Power cord L-2100/2130

Less than 100 ohms terminal
More than
750 mm
under ground
Ground wire
(green)
Copper plate
300 mm Ground Connector
2 mm
10 mm
2.3.2 Installation Space For installation of this system, it is required to provide a space of
at least 640 mm (width) × 550 mm (depth). Prepare a sturdy,
level desk or bench capable of supporting a weight load of at
least 150 kg. On the rear of the main unit, allow at least
100 mm of clearance.
NOTICE: Installation of the instrument shall be carried out by

or under supervision of qualified service personnel
of the manufacturer or its authorized service agent.
Before installation of the instrument, the customer
is requested to prepare necessary utilities referring
to this manual.
When relocation of the instrument becomes
necessary after initial installation (delivery), please
notify your local Hitachi High-Technologies sales
representative or service office.
2.4 Checking the Contents
After unpacking, check the contents of each case against the

packing list attached to it.
2-5

2.5 Assembly
2.5 Assembling
2.5.1 Power Supply The L-2000 Series system is provided with a system power
Unit supply unit which is capable of connecting two pumps, one
autosampler, and one detector. The column oven has its own
power cord. In an instance where provision of a single system
power supply unit is insufficient for the use of two detectors, use
an AC adapter suitable for each instrument (50 W AC adapter
for the pump/autosampler, 100 W AC adapter for the detector).
The power cable from the system power supply unit and the AC
adapter power cable should be connected to the power
connector equipped on the left rear side of the instrument.
Rear of e-Line connector

instrument
Power connector
2-6

2.5.2
(1) Isocratic System/Low-Pressure Gradient System
Connect the power cables of the system power supply unit

and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50 W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.
System power
supply nit
100 W cable
Model L-2400 UV
detector
Model L-2300
column oven
Model L-2100 50 W cable

pump
Model L-2100/2130
pump
To AC power
source
2-7

2.5 Assembly
(2) High-Pressure Gradient System
Connect the power cables of the system power supply unit

and column oven to AC power source. For the L-2100/
2130 pump and L-2100 pump, connect the 50 W cable
from the system power supply unit to the instrument power
connector. For the L-2400 UV detector, connect the 100
W cable from the system power supply unit to the
instrument power connector.
100 W cable
Model L-2400
UV detector
L-2300 column oven 50 W cable
Model L-2100/
2130 pump
Model L-2100
pump
Model L-2100/
2130 pump
To AC power
source
2.5.3 Wiring In the L-2000 Series, connect the e-Line cable to the e-Line
Arrangement connector equipped on the left rear of each instrument for
instrument-to-instrument wiring connection.
Rear of e-Line connector

instrument
Power connector
2-8

2.5.3
(1) Isocratic System/Low-Pressure Gradient System
e-Line cable
e-Line cable
Model L-2100 pump
e-Line cable
Model L-2400 UV detector e-Line cable
System power
Model L-2300 column oven supply unit
e-Line cable
Model L-2100 pump

e-Line cable
Model L-2100/
2130 pump
2-9

2.5 Assembly
2.5.4 Piping In the L-2000 Series, provide inlet tubing to the pump and waste
Arrangement solution tubing through the right piping section of the drain
reservoir. Provide tubing from the pump to the autosampler,
column and detector through the center piping section of the
drain reservoir.
Drain reservoir
Center Piping Section

Run tubes from the pump to the autosampler,
column and detector.
Right Piping Section

Run inlet tubing to the pump and waste
solution tubing.
2 - 10

2.5.4
(1) Isocratic System
Connect the inlet tube of eluent to the IN port of the

degasser. Connect the OUT port of the degasser to the
inlet of the pump. Connect the drain valve of the pump to
port 1 of the injection valve of the autosampler.
Connect port 2 of the injection valve to the column inlet.
Connect the column outlet to the inlet tube of the detector
cell. For washing solution, provide connection to port 1 of
the syringe valve of the autosampler through the degasser.
Washing solution
Eluent
Inlet tube
Detector cell

Column
Injection valve
Model L-2100 pump

Syringe valve
Degasser
Drain
Drain valve
2 - 11

2.5 Assembly
(2) Low-Pressure Gradient System

degasser. Connect the OUT port of the degasser to the IN
port of the proportioning valve. Connect the OUT port of
the proportioning valve to the four-way joint.
Connect the mixer of the pump to port 1 of the injection
valve of the autosampler. Connect port 2 of the injection
valve to the column inlet. Connect the column outlet to the
inlet tube of the detector cell. For washing solution,
provide connection to port 1 of the syringe valve of the
autosampler through the degasser.
Eluent
Washing solution
Inlet tube
system power supply unit
Detector cell
Column
Injection valve
Model L-2100 pump
Syringe valve
Drain valve
L-2100/2130 pump
Degasser
Mixer Drain
Proportioning valve
Four-way joint
2 - 12

2.5.5

degasser. Connect the OUT port of the degasser to the IN
port of the pump. Connect the drain valve of the pump to
the mixer. Connect the mixer of the pump to port 1 of the
injection valve of the autosampler. Connect port 2 of the
injection valve to the column inlet. Connect the column
outlet to the inlet tube of the detector cell.
For washing solution, provide connection to port 1 of the
syringe valve of the autosampler through the degasser.
Column Detector cell

Inlet tube
Eluent
Washing solution Model L-2400 UV detector
System power supply

unit Model L-2300 column oven
Injection valve
Syringe valve
Model L-2100/
2130 pump
Model L-2100 pump
Drain
Drain valve
2.5.5 Module Unit Where the chromato data station is not used for controlling
Operating operations, each module can be operated using the optional UI
Procedure pad.
This section describes how to use the UI pad for operations.
The UI pad is provided with common control keys available for
different modules and unique control keys for particular modules.
The functions of the common control keys are mentioned below.
2 - 13

2.5 Assembly
(1) Using the UI Pad
(a) Functions of common control keys
LOCK OFF
7 8 9 0 ESC
4 5 6 DEL
SET UP INSERT
UTLTY GLP PROG 1 2 3 CL ENT
Key Designation Function

UTLTY Starts instrument parameter setting.
GLP Starts GLP function setting. Available for displaying a logbook of component
parts of each module, triggering a reset, and locking the UI pad keys.
PROG Starts program editing.
ESC Returns control to the monitor window.
DEL Deletes a cursor-pointed step.
This key is effective only in program editing.
When the cursor is positioned on the TIME input part, pressing the DEL key
removes a step pointed by the cursor. When the cursor is positioned on
another input part, pressing the DEL key removes an item pointed by the cursor.
Pressing the ESC key on a window where "-" is indicated returns control to the
monitor window, causing cancellation of a DEL key action.
• Pump/detector time program
When the DEL key is pressed, "-" is indicated. This deletion can then be
defined by pressing the ENT key.
• Autosampler sequential program
When the DEL key is pressed, the relevant step number is deleted and "-" is
indicated. This deletion can then be defined by pressing the ENT key.
CL Clears an input numeric value during input (after a numeric value or the decimal
point is entered; before the ENT key is pressed).
The cursor is indicated at the top of the input part. The previous input value is
presented as a preset value.
ENT Defines the current entry of a parameter.
INSERT Use this key in program editing.
• Pump/detector
A new line is presented following the last line of the current program.
• Autosampler
A new step is inserted.
, (1) Monitor window
(Arrows) • key : Goes to the next window.
• key : Goes back to the previous window.
(2) Program editing
• key : The next line is presented. Pressing this key at the last line
causes the first line to appear in a circular fashion.
• key : The previous line is presented. Pressing this key at the first
line causes the last line to appear in a circular fashion.
2 - 14

2.5.5
(b) Contents on screen

The parameter input window and the program setup
window are described below.
1) Input of a single numeric value:
Parameter name Input range
PROGRAM No. (1 - 9)
1
Input part
2) Input of plural numeric values
Parameter name Input item
LAMP CHANGE (MM, DD, YYYY)

<1 - 12>
Input range Input part
3) Selection of a numeric value:
Parameter name Selectable items
TIME CONSTANT (0.1s = 1, 0.5s = 2, 2.0s = 3,

3 4.0s = 4, 8.0s = 5)
Input part
4) Program setup window:
Input item
NO. R.NO VIALS INJ.VOL INJ/VIAL S.TIME

1 1 80 50 1 10.0
Input part
2 - 15

2.5 Assembly
(c) Numeric Value Input Procedure

1) The cursor is indicated at the top of the input part.
The previous numeric value is presented in the
input part.
2) Numeric and decimal point key inputs are
accepted in each allowable range.
3) To define an input numeric value, press the ENT
key. The cursor will then move to the next item.
If an erroneous numeric value is input, the cursor
is brought back to the top of the input part with
the input numeric value displayed. In this state,
you are prompted to enter a correct numeric value.
Pressing the ESC key brings you back to the
initial window (monitor window).
If the ESC key is pressed during numeric key
input, the parameter being entered is discarded
(the previous numeric value is set).
NOTICE: At power-on, a default number is indicated in each

item selection part.
Pressing the CL key in the input part clears its contents.

The cursor is then brought back to the top of the input part.
In program editing, pressing the DEL key in the input part
removes an item pointed by the cursor.
If the ESC key is pressed in the course of setting plural
parameters, the items preceding the press of the ESC key
remain intact.
2 - 16

2.5.5
(2) Mounting the UI Pad
(a) Remove the front panel from the instrument.
Front panel
(b) Remove the metal lid from the instrument.
Meal cover
2 - 17

2.5 Assembly
(c) Run the flexible tube of the UI pad through the metal
lid.
Metal cover
Flexible tube
UI pad
(d) Turn up the lid of the connector, and insert the flexible
tube of the UI pad as far as it will go.
Flexible tube
Connector
UI pad
2 - 18

2.5.5
(e) Turn down the connector lid, and mount the UI pad.
Flexible tube
Connector
UI pad
(f) Attach the metal lid dedicated for the UI pad.
Flexible tube
Metal cover
UI pad
2 - 19

2.5 Assembly
(g) Set the of the UI pad to the of the instrument.
Tab
UI pad
(h) Mount the front panel dedicated for the UI pad on the
instrument.
Front panel
2 - 20

2.5.6
2.5.6 Instrument-to- The wiring cover is provided on the rear of each instrument.
Instrument Instrument-to-instrument fastening can be made by shifting each
Fastening wiring cover as shown below.
(1) Remove the retaining screws, and detach the wiring cover.
Wiring cover
Retaining
screw
(2) Shift the mounting position of each wiring cover.
Wiring cover
Retaining
screw
2 - 21

3. FUNCTION
3.1 The Instrument Keypad
3.1.1 Name and

Function of Each
Part
Main switch Right door
LED
Drain Valve Left door Drain

Leak sensor
Fig. 3-1 Front View
• Main switch : Turns on/off power supply.

• Doors : Two doors are provided.
• Drain Valve : This valve is opened for purging the system.
• Leak sensor : Detects fluid leakage.
• UI-PAD : A keypad required for operating the pump
independently (available at option).
• LED : There are 4 LEDs, and each LED lights in a
specific color for the determined indication.
Name (color) LED Status

A (green, orange) Green : POWER ON as the pump A
Orange : Pump A error
B (green, orange) Green : POWER ON as the pump B
Orange : Pump B error
PURGE (green) Green : Under purge
PROG. RUN (green) Green : Under the time program

running
3-1

3.1.1
I/F board mounting

Wiring covers space
Ground terminals
Wiring covers
Pump can start by an Pump can stop by an Pressure value is output

external signal. external signal. to the exterior (Approx.
49 MPa)/1 V.
Fig. 3-2 Rear View
Wiring covers : Upon detaching the left-side cover (as

viewed from the front), the e-Line
connector used for connection between
L-2000 series units appears.
These wiring covers are also usable as
retaining plates for other units of the
L-2000 series.
Ground terminals : Three terminals for grounding the
instrument. I/F board mounting
IF board space : Space for mounting the I/F board required
for connecting the PC (data station) and
HPLC system.
3-2

3.10
Table 3-1 Name and Function of Each Terminal at Rear Panel
Classi- Contents of Contents of

Name Function Remarks
fication Signal Processing
Output TIMER Contact • Timer The ON/OFF • TIMER No. and Event code
(1 to 4) turns signal can
on/off ON: CLOSE output to
according each TIMER by
to coded specifying an TIMER Signal
setting in CLOSE ON/OFF EVENT Pulse
No. OFF ON
EVENT of code at a
the OFF: OPEN desired time
program. of a time 1 10 11 12
program. 2 20 21 22
OPEN 1-second 3 30 31 32
CLOSE signal
can output at 4 40 41 42
each TIMER by • Pump can be stopped by specifying
• Pulse specifying 00 for EVENT, and buzzer can be
pulse EVENT rung by specifying 92.
OPEN code at a
desired time
of a time 1) Becomes OFF when the pulse is
program. output in the ON status.
2) OFF has priority over ON.
1 s 3) Contact output specification of
each terminal
CLOSE Voltage ... 30 V DC max.
Current ... 100 mA max.
PRESS Pressure 0 to 1 V DC • 1 V = Approx. 49 MPa
OUT value is (Approx. 7106 psi)
output to
the
exterior.
Input PUMP Pump ON OPEN ∗ Maximum current connectable: 10 mA
ON IN from ∗ Edge jugement
external
system. 50 ms
or longer
PUMP Pump OFF
OFF IN from
external
system.
3-3

3.1.2 Part Name and
Function of UI (1) Operation Keys
Pad (option)
Each key on the UI pad (option) type operation panel is
explained here.
START STOP MANUAL LOCK OFF
7 8 9 0 ESC
A B C D
4 5 6 DEL
PUMP PURGE SET UP INSERT INITIAL
UTLTY GLP PROG 1 2 3 CL ENT
PUMP Starts/stops operation of the pump. Pressing this key

ON/OFF starts the pump.
Then, pressing it again stops the pump.
PURGE Starts/stops purging the flow path.
For specifying a purging flow rate, use the UTILITY
function.
The upper pressure limit (PRESS MAX) is set at 2.9
MPa (30 kgf/cm2), and the lower pressure limit
(PRESS MIN) is set at 0 MPa (0 kgf/cm2).
Pressing this key again stops the purging operation.
UTLTY 1.Used to display/set up auxiliary function conditions
/ (UTILITY).
SET UP • Pressure zero-point correction
• Purge flow rate setting
• Pressure unit assignment
2.For pump mode selection.
The low-pressure gradient mode, high-pressure
gradient mode, and single-liquid feed mode are
available (SET UP).
GLP Used for confidence-level reporting.

PROG Selects, generates, edits, or deletes a pump program.
, Scroll up and down display.
START Starts the time program. You can activate the start key
only when the pump is at the initial mode.
STOP Stops the time program.
MANUAL Used for manual operation selection display and
setting (mobile phase mixing ratio, FLOW, P-MAX, P-
MIN, %B, %C, %D).
ESC 1. Pressing this key brings you from the numeric value
/ input state (input state in each mode) back to the
LOCK MONITOR screen.
OFF 2. For key unlocking.
A,B,C,D If you are in manual status and you press the [A]
key %A is set immediately to 100. [B], [C], and [D]
keys provide the same function.
INITIAL Puts the systems to the initial setting of program
whose no. is selected by [PROG] key (time program: 0
minutes)
3-4

CL Used for erasure of data under input. Also usable for
erasure of an error message at occurrence of an error.
DEL Used for deleting a step in a sequential program.
ENT Inputs a numeral.
NOTE: If a mistake is made during operation, press the

ESC key. In almost all cases, the initial status
returns when pressing this key.
(2) Monitor Screen
The UI pad is provided with an LCD monitor on which

instrument status can be indicated. The screen which
indicates instrument status is called a monitor screen.
Operation method of the monitor screen and example
indications on it are shown below.
(a) 1st monitor screen

This screen mainly indicates information about the
pump main frame.
TIME %A %B %C %D FLOW PRESS [--]

0.0 100 0 0 0 0.000 0.0 1
Indication Description
TIME Elapsed time of analytical program execution
%A Mixing ratio of liquid A
%B Mixing ratio of liquid B
%C Mixing ratio of liquid C
%D Mixing ratio of liquid D
FLOW Pump flow rate
PRESS Pump pressure
[--] Pump status indication
Since two-liquid low-pressure gradient operation is performed in

this system, %C and %D are always zero.
The status indications at the upper right of the

MONITOR screen are as follows:
[GR] The gradient program is in execution.
3-5

[IN] Waiting for execution of analysis. A liquid is being fed.
[--] Waiting for execution of analysis. The pump is being
stopped.
[MN] The MANUAL mode is selected.
[PG] The purging operation is under execution.
[LK] The keyboard of the operation panel is locked.
(b) 2nd monitor screen
P-UNIT P.MAX P.MIN [--]

MPa 20.0 0.0 1
P-UNIT Indicates a unit of pressure. In this system, MPa is
indicated.
P. MAX Indicates a specified upper limit of pressure. If
pressure exceeds the specified upper limit, the pump
is forced to stop.
P. MIN Indicates a specified lower limit of pressure. If
pressure decreases below the specified lower limit,
the pump is forced to stop.
(c) 3rd monitor screen
EVENT 1 2 3 4 PUMP FLUSH [--]

OFF OFF OFF OFF A(Micro) OFF 1
EVENT Indicates a state of the EVENT terminals 1 to 4. ‘ON’
represents that the contact is closed, and ‘OFF’
represents that the contact is open.
PUMP Indicates a pump station number. In pump station
number assignment, PUMP A, PUMP B or PUMP C
can be specified.
FLUSH Always indicated as ‘OFF’ in this system.
3-6

(c) Changeover of monitor screen
The monitor screen transition is as follows. If the link program
function is OFF, the 4th monitor screen will not appear.
1) 1st MONITOR screen
TIME %A %B %C %D FLOW PRESS [--]

0.0 100 0 0 0 0.000 0.0 1
2) 2nd MONITOR screen

or
P-UNIT P.MAX P.MIN [--] ESCAPE
MPa 20.0 0.0 1
3) 3rd MONITOR screen
EVENT 1 2 3 4 PUMP FLUSH [--]

OFF OFF OFF OFF A(Micro) OFF 1
4) 4th MONITOR screen
LINK PROG STEP CYCLE [--]

ON 1 1/99 1
3-7

3.2 Operation Status
Fig. 3-3 is a flow chart that shows the transition of operational
status. ( indicates depression of a key.)
<Turning on power>
(does not function unless the pump is on)
START/STOP
INITIAL RUN
START/STOP
MANUAL MANUAL
INIT SET SET
MANUAL
INITIAL : Programming mode status in which

system waits for start.
MANUAL : Operation is made under constant
conditions without using program.
RUN : Gradient mode is executed.
Fig. 3-3 Operational Status Transition
3-8

3.3 Specifications
(1) L-2100
(a) Liquid feed system:
Dual plunger type reciprocating pump( Tandem Type)
(b) Maximum operating pressure:
39.2 MPa
(c) Flow rate range
0.001 to 2.499 mL/min
(d) Pressure limit range
0 - 39.2 MPa
(e) Delivery volume of No.1 plunger stroke
25 uL/stroke
(f) Flow accuracy (at constant room temperature of
20 for water)
+/- 2 uL/min (0.005 - 0.050 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (0.051 – 2.000 mL/min, 1.0 - 39.2 MPa)
+/- 4 % (2.000 - 2.499 mL/min, 1.0 - 39.2 MPa)
(g) Flow precision
0.075% RSD or 0.02min SD, whichever is greater,
based on retention time using L-2200 autosampler
(Flow 0.2 mL/min at constant room temperature of
20 for water )
(h) Pressure display accuracy
+/- 5%
(i) Constant pressure liquid feed
2.0 to 39.2 MPa (Flow range 0 to 1.25 mL/min)
(j) Material of wetted parts
SUS 316, ruby, sapphire, ceramic, PTFE,
carbon-impregnated PTFE
(k) Display data using UI-Pad (Option):
Parameters
Gradient monitor Elapsed time, mixing ratio, flow rate,
pressure, timer value, etc.
(l) Pump control mode:
Single-Liquid delivery mode
Low pressure gradient mode
High pressure gradient mode
(m) Contact input/output terminals:
Event timer; 4 channels
(n) Communication:
e-Line
OUTPUT: BUSY
INPUT: 1) GRADIENT START IN
2) ERROR IN
(o)Pressure zero adjustment:
3-9

Possible
(p) Ambient temperature :
4 to 35°C (condensation unallowable)
(q) Humidity:
45 to 85% RH
(r) Power supply:
24V DC, 21.A
(s) Dimensions:
260(W) × 500(D) × 235(H) mm
(t) Weight:
About 15 kg
(2) L-2130
(a) Liquid feed system:
Dual plunger type reciprocating pump( Tandem Type)
(b) Maximum operating pressure:
39.2 MPa
(c) Flow rate range
0.001 to 9.999 mL/min
(d) Pressure limit range
0 - 39.2 MPa
(e) Delivery volume of No.1 plunger stroke
25 uL/stroke
(f) Flow accuracy (at constant room temperature of
20 for water)
+/- 2 uL/min (0.01 - 0.10 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (0.101 – 5.000 mL/min, 1.0 - 39.2 MPa)
+/- 2 % (5.001 – 8.000 mL/min, 1.0 – 19.6 MPa)
+/- 4 % (8.001 - 9.999 mL/min, 2.0 – 19.6 MPa)
(g) Flow precision
0.075% RSD or 0.02min SD, whichever is greater,
based on retention time using L-2200 autosampler
(Flow 1.0mL/min at constant room temperature of
20 for water )
(h) Pressure display accuracy
+/- 5%
(i) Constant pressure liquid feed
2.0 to 39.2 MPa (Flow range 0 to 5.0 mL/min)
(j) Material of wetted parts
SUS 316, ruby, sapphire, ceramic, PTFE,
carbon-impregnated PTFE
(k) Display data using UI-Pad (Option):
Parameters
Gradient monitor Elapsed time, mixing ratio, flow rate,
pressure, timer value, etc.
3 - 10

(l) Pump control mode:
Single-Liquid delivery mode
Low pressure gradient mode
High pressure gradient mode
(m) Contact input/output terminals:
Event timer; 4 channels
(n) Communication:
e-Line
OUTPUT: BUSY
INPUT: 1) GRADIENT START IN
2) ERROR IN
(o)Pressure zero adjustment:
Possible
(p) Ambient temperature :
4 to 35°C (condensation unallowable)
(q) Humidity:
45 to 85% RH
(r) Power supply:
24V DC, 21.A
(s) Dimensions:
260(W) × 500(D) × 235(H) mm
(t) Weight:
About 15 kg
3 - 11

4. OPERATION
The method of pump operation using the optional UI pad is

described here.
4.1 Preparatory Operations
Explained here is the operating method for using the L-2130

pump.
4.1.1 Setting of Pump Mode
This section shows how to set the pump in the single-liquid

delivery mode (FLOW mode).
NOTICE: Once this setting is made, it is memorized and need not

be made each time.
<POWER ON> + [(SETUP) UTILITY]

When pressing the [POWER] and [(SETUP) UTILITY]
keys simultaneously, the pump mode setting screen
appears. It may be necessary to continue pressing
[(SETUP) UTILITY] until the setting screen appears.
SETUP PUMP MODE CONTRAST S.NO.

A LOW 4 02AB-111
[ENTER]
SETUP ITEMS PUMP=1 CONTRAST =2
[1][ENTER]
PUMP A=1 B=2
[1][ENTER]
4-1

GR MODE LOW HIGH V. HIGH
4 FLOW 4
[4][ENTER] LOW: Low pressure gradient mode

HIGH: High pressure gradient mode
V.HIGH: High pressure gradient with
solenoid valves mode
FLOW: Isocratic mode
SETUP PUMP MODE CONTRAST S.NO.
A FLOW 4 02AB-111
<POWER OFF>
Turn the power off once.
<POWER ON>
Turn the power on again.
TIME FLOW PRESS [ ]

0.0 1.000 0.0 1
4-2

4.1.2 Programming Operation
This section discusses the programming of the FLOW mode.

An example is given where upper pressure limit is 20.0 MPa,
lower pressure limit is 0 MPa, analysis time is 0 min, flow rate is
1.0 mL/ min.
PROGRAM No. = 1
PRESSURE VALUE : MAX MIN MPa

20.0 0.0
TIME FLOW EVENT

(min) (ml/min) 1 2 3 4
0.0 1.000 12 22 32 42
Monitor screen
If manual setting screen is displayed, press INITIAL to

obtain the programming mode.
PROG
Program generation is started.
SET PROGRAM: No. [--]

<1-9> 1
Input program No. Valid program numbers are from 1 to

9.
MODIFY=0, NEW=1 [--]

0
Generates a new program or modifies an existing

program.
4-3

PRESSURE: MAX MIN [--]
20.0 0.0 1
Input the upper and lower limit pressure values.
TIME FLOW EVENT [--]

0.0 1.000 1
Input time, flow rate and event. Up to 4 entries can be

made per step for event. Input an event to be
executed within the entered time.

1
A new input screen will appear, so enter time, flow

rate and event.
[ ESCAPE ]
Monitor screen
NOTICE: A maximum of 99 steps can be entered for program

Nos. 1 to 9.
NOTICE: The entered program is saved. When power is turned on

next time, the initial screen (TIME=0) of the program used just before
power was turned off will appear.
4-4

4.1.3 Manual Operation
This is used for supplying liquid under constant conditions

without using a program.
(1)Constant Flow Rate Mode
This section explains the setting of liquid delivery system at

constant flow rate. An example is given where flow rate at 1.0
mL/min, upper pressure limit at 15.0 MPa and lower pressure
limit at 0 MPa.
Monitor screen
MANUAL
MANUAL: FLOW=1, PRESS=2 [MN]

1
Select the constant flow rate mode.
CONSTANT FLOW: FLOW MAX MIN [MN]

1.000 15.0 0.0
Input the flow rate, and the upper and lower limit pressure
values.
CONSTANT FLOW: FLOW PRESS [MN]

0.000 0.0
The monitor screen will apper.
Monitor screen
NOTICE: The set conditions for manual operation will be erased

when power is turned off. Conditions must be set each time power
is turned on.
4-5

(2)Constant Pressure Mode
This section explains the setting of liquid delivery system at

constant pressure. An example is given where initial flow rate is
1.0 mL/min, at a pressure of 15.0 MPa.
Monitor screen
MANUAL
MANUAL: FLOW=1, PRESS=2 [MN]

2
Select the constant pressure mode.
CONSTANT PRESS: INITIAL FLOW PRESS [MN]

1.000 15.0
Input the flow rate, and the pressure values.
CONSTANT FLOW: FLOW PRESS [MN]

0.000 0.0
The monitor screen will apper.
Monitor screen
Before stating the pump, check that the pressure level is 0 Mpa.
NOTICE: The liquid delivery will not start unless the starting pressure
is 0 MPa
NOTICE: The set conditions for manual operation will be erased when
power is turned off. Conditions must be set each time power is turned
on.
4-6

4.2 Measuring Operation
WARNING
Ignition of Flammable Chemicals!
• Beware of ignition hazard when using flammable chemicals such

as organic solvents.
• Always check the following conditions. If an abnormality is
found, stop operation immediately.
• Leakage of solvent or waste solution
• Leakage of solvent inside the instrument
• Inadequate ventilation of the laboratory room
• This instrument is not explosion-proof. Although aqueous
solvents or organic solvents having an ignition point of 70°C or
higher are usable, do not use organic solvents having an ignition
point below 70°C.
• When using flammable chemicals, be careful about possible
ignition due to static electricity. Particularly when using non-
conductive chemicals, employ a conductive vessel made of metal
or the like and provide grounding connection correctly.
WARNING
Explosion of Vapor from Flammable Chemicals!
• If a flammable chemical such as organic solvent leaks from the

flow path of the instrument and its vapor concentration exceeds
the explosion limit, it may cause spontaneous combustion with
dangerously explosive results.
• When using a flammable and readily volatile chemical, be sure to
check for leakage from the instrument flow path and ventilate the
laboratory room adequately.
4-7

4.2.1 Basics of Operation
When the settings are all finished, carry out operation in the
following procedure.
(1) Make sure the pump, column, detector and data

processor are properly connected.
(2) Set the mobile phase and waste solution bottle in place.
(3) Start the pump and wait until the flow path is filled with
the mobile phase.
(4) Warm up the pump, column and detector and wait until
the baseline stabilizes.
(5) Inject a sample.
(6) When analysis is finished, turn the power off and dispose
of the waste solution.
NOTICE: Before connecting a new column, make sure the flow

path is filled with mobile phase so that air won’t enter the
column.
NOTICE: The whirring sound of the pump during operation

varies depending on such operating conditions as flow rate and
pressure. Although the whirring sound may become larger
anywhere in the high-pressure range, it is not a symptom of
abnormality.
4-8

4.2.2 Analyzing Operation
(1) SET PROGRAM allows the generation of a maximum of 9 programs.

You can execute any of the programs selected or generated via the SET PROGRAM.
For a gradient analysis, implement the following procedure.
(a) Select [INITIAL] , and select or generate a program for eluent.
(b) Turn on each pump.
(c) Start the program.
(2) Contents of SET PROGRAM
For the SET PROGRAM, enter the upper limit pressure level, lower limit pressure
level, mixing ratio, flow rate, and event.
PROG No.=1
PRESSURE MAIN MAX MIN (MPa)

20.0 0
(a)
TIME FLOW EVENT
0.0 1.000
10.0 1.000 1121 31 41
10.1 1.000
20.0 1.000
(b) 25.0 10 20 30 40
30.0 2.000
30.1 1.000
40.0 1.000 00 92
Fig. 3-4 Example of Program
(a) For EVENT, up to 4 event codes are settable at each time point. It is
possible to generate contact outputs at each EVENT terminal at the same
time. You can also obtain an internal action, such as a pump stop and the
ringing of a buzzer.
Example) EVENT= 1 - 1 - ENTER - 2 - 1 - ENTER
4-9

3.9
(b) In case that an event setting is desired independent of FLOW , only the event
code showed be entered. Press ENTER to skip all the entries you do not
want until you reach event column.
In the SET PROGRAM mode, the interpolation of mixing ratio, flow rate, etc. that
occurs in a time program is as follows.
Parameter Flow Rate

Interpolation Linear
TN TN+1
TN, TN+1: Preset time
NOTICE: For generating a stepwise gradient program, utilize TN+1 - TN = 0.1.
4 - 10

(1) New Generation of a Program
This section shows the generation of a new program.
1
SET PROG (SET PROG No.) No. of program to be ENTER
generated (m)
2
(MODIFY=0, NEW=1) 1 ENTER
3 4
(MAX) Upper limit pressure ENTER (MIN) Lower limit pressure ENTER
level level
5
(TIME) Set time (Ti) ENTER
6
(FLOW) Set flow rate (Fi) ENTER
7
(EVENT) Event code (E1i) ENTER
7
Event code (E2i) ENTER
7
7
8 Repeat steps 5 to 7 until you define all programs lines.
9 To terminate the program generation press [ESC]
4 - 11

1 Program number can be from 1 to 9.
2 When you want to define a new program, enter 1.

As a result, you will delete the previous program.
For modifying the contents of program, enter 0.
3 If pump pressure rises beyond the level set here, the pump automatically stops.
4 If pump pressure falls below the level set here, the pump automatically stops.
5 The set time can be from 0.0 to 600.0.
6 Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are
from 0.00 to 9.999 (L-2130).
7 Input the event code. At the set time point, you can stop the pump, generate an
audible signal, markers, timers, etc. When properly interfaced, these signals can
be output to an external system. No display will show on the screen unless an
event is set.
8 Repeat steps 5 to 7 until you complete the program and then press [ESC] .
9 Program generation will be terminated and the monitor screen will reappear.
For confirming the input conditions, check the parameters at each time point by using
keys.
4 - 12

(2) Deletion of Program
How to delete a program is shown next.
1
SET PROG (SET PROG No.) No. of program to be
deleted (m)
2 3
ENTER (MODIFY=0, NEW=1) 1 ENTER ESCAPE
(3) Deletion of Step
How to delete one step in a program is shown next.
SET PROG (SET PROG No.)
1
No. of program containing step to be ENTER (MODIFY=0, NEW=1)
deleted (m)
2 3
0 ENTER (MAX) Upper limit pressure ENTER (MIN)
level
4 5 6 7
Lower limit pressure ENTER DELETE ENTER ESCAPE
level
4 - 13

2 The contents of the existing program will be deleted.
3 The monitor screen reappears.
2 For editing, input 0. For generating a new program, input 1.
.
5 Press the key until the step you want to delete appears on the screen. If display
advances beyond that step, press the
key.
6 Press the [DEL] key when the cursor is on the TIME line want to delete.
7 To finish editing, press the [ESC] key. The display should then return to the
monitor screen.
4 - 14

(4) Addition of Step
1
SET PROG (SET PROG No.) No. of program for adding ENTER
a step (m)
2
3 4
level level
5
(TIME) Time point of step added (Ti) ENTER
6
7
7
7
7
4 - 15

2 For editing, input 0. For generating a new program, input 1.
.
4 If pump pressure falls below the level set here, the pump automatically stops. The
.
5 Input the time point of the step you want to add.
6 Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are
from 0.00 to 9.999 (L-2130).
7 Input the event code. At the set time point, you can stop the pump, generate an
audible signal, markers, times, etc. When properly interfaced, these signals can be
output to an external system. No display will show on the screen unless an event is
set.
4 - 16

(5) Correction of Step
Step correction method is explained here.
1
No. of program containing step to be ENTER
deleted (m)
2
3 4
level level
5 6
(TIME) Time point of step added (Ti) ENTER
7
8
8
8
8
4 - 17

2 For correction, input 0. For generating a new program, input 1.
3 If pump pressure rises beyond the level set here, the pump automatically stops. ]
4 . If pump pressure falls below the level set here, the pump automatically stops.
5 Press the key until the step to be corrected appears on the screen. If the display
advances beyond that step, press the key.
6 When a correction is unnecessary, press the ENTER key only. The allowable
input range is 0.00 to 2.499.
7 When a correction is unnecessary, press the ENTER key. Valid flow is from 0.00
to 2.499(L-2100). Valid flow is from 0.00 to 9.999(L-2130).
8 Enter the event code. When a correction is unnecessary, press the ENTER key.
4 - 18

(6) Deletion of Parameter
The parameter deletion method is shown next.
1
No. of program containing parameter to ENTER
be deleted (m)
2
3 4
level level
5 6 7
ENTER DELETE ENTER
4 - 19

2 For correction, input 0. For generating program, input 1.
5 Press the key until the step to be corrected appears on the screen. If display
advances beyond that step, press the
key.
6 Move the cursor to the parameter to be deleted.
7 To delete the parameter, press the DELETE key and then press ENTER key.
4 - 20

4.3 Setting of Utility Functions
Utility functions such as zero adjustment of pump pressure, selection of pump pressure unit
and column flushing are settable as follows.
4.3.1 Pressure Zero Adjustment
You do not need to perform a pressure zero adjustment every day. The zero point may
change due to a secular change of the pressure sensor, etc. Check the pressure zero point
once a month. If it has drifted, then stop the pump, open the drain valve and carry out the
following operation.
Monitor screen
UTLTY
UTILITY CONDITION=1 UNIT=2 FLUSH=

1 EVENT 4 LINK= OTHERS=6
Select CONDITION .
CONDITION SET Z-ADJ=1 MOTOR-CTRL=2

1 PURGE=3, CALIBRATION=4
Select Z-ADJ (PRESS ZERO ADJUST).
PRESS ZERO ADJUST: YES=1, NO=0 [--]

(OPEN DRAIN VALVE) 0
Open the drain valve, and then enter 1.
Monitor screen
PRESS indication becomes “0” and the pressure zero adjustment is finished.
4 - 21

4.3.2 Pump Motor Control Method
Automatic flow rate control method (STANDARD) and constant flow rate control method
(FIX) are available as pump motor control methods.
Monitor screen
UTLTY

EVENT 4 LINK= OTHERS=6
Select CONDITION .

Select MOTOR-CTRL (MOTOR CONTROL).
MOTOR CONTROL: STANDARD=1, FIX=2 [--]

1
STANDARD
Monitor screen
MOTOR CONTROL is set to STANDARD.

FIX
FIX STEP AREA

<0, 30-300> 100
Enter a FIX STEP AREA.

Input range is 0, and from 30 to 300.
Monitor screen
NOTICE: Select “STANDARD” for Model L-2100/2130 pump.
4 - 22

4.3.3 Purge Flow Rate
Set a flow rate when executing purge.
Monitor screen
UTLTY

Select CONDITION.

Select PURGE.
PURGE FLOW: FLOW [--]

<0.001-2.499> 2.499
Enter a purge flow rate.
Monitor screen
Pressing the Purge key in PUMP ON status activates purging at the set flow rate. Pressing
the Purge key again during purge will reset the purge function. During purge, the upper
pressure limit is set at 2.9 MPa (30 kgf/cm2) and the lower pressure limit at
0 MPa (0 kgf/cm2).
4 - 23

4.3.4 Flow Calibration
Set a parameter of flow rate calibration.
Monitor screen
UTLTY

Select CONDITION.

Select CALIBRATION.
CALIBRATION FLOW=1 PRESS=2
Select FLOW.
FLOW CALIB. 0.97 – 1.03

1.000
Enter a parameter o flow rate calibration
Monitor screen
4 - 24

4.3.5 Pressure Calibration
Set a parameter of pressure calibration.
Monitor screen
UTLTY

EVENT 4 LINK=5 OTHERS=6
Select CONDITION.

Select CALIBRATION.
CALIBRATION FLOW=1 PRESS=2

2
Select PRESS.
FLOW CALIB. 0.900 – 1.100

1.000
Enter a parameter of pressure calibration
Monitor screen
4 - 25

4.3.6 Pressure Unit
Pump pressure unit is selectable from bar, psi, kgf/cm2 and MPa.
Monitor screen
UTLTY

Select UNIT.
PRESS UNIT: <bar=1, psi=2, kgf/cm2=3, [--]

<1-4> 1 MPa=4>
Select pressure unit.
Monitor screen
Pressure indication unit is set to selected one.
The set pressure unit is retained in memory even after turning off power supply. The
relationship among selectable pressure units is listed below.
bar psi kgf/cm2 MPa

10.0 145 10.2 1.00
4 - 26

4.3.7 Column Flushing
Automatic pump stop and/or column flushing after completion of analysis is settable.
By setting for column flushing, the flushing will be done automatically after the end of
analysis. When a salt solution has been used for analysis, for example, elution of the salt
component can be prevented by flushing the column with water.
An example is given where the column is flushed for 30 minutes with 100% liquid C 15
minutes after the end of analysis.
Monitor screen
UTLTY

Select FLUSH.
PUMP AUTO STOP: YES=1, NO=0 [--]

1
Select YES (PUMP AUTO STOP).
COLUMN FLUSH: YES=1, NO=0 [--]

0
[YES]
SET DELAY TIME: TIME (min) [--]
<1.0-600.0> 600.0
Input the time point of pump stop after completion of

analysis.
Monitor screen
4 - 27

FLUSH MODE: PARAM.=1, PROG.(NO.9)=2 [--]
1
Enter “1” for column flushing under constant conditions, or “2” for column flushing
using a gradient.
[PARAM]
SET DELAY TIME: TIME (min) [--]
<1.0-600.0> 600.0
Input the time period from the end of analysis to the

start of column flushing.
COLUMN FLUSH: FLOW [--]

1.000
Input column flushing conditions (flow rate).
FLUSHING TIME: TIME (min) [--]

<1.0-600.0> 30.0
Column flushing time can be input within 1.0 to 600.0

min.
Monitor screen
[NO]
Monitor screen
When the time for column flushing arrives, the flushing is

executed according to No.9 program.
NOTICE: The program for column flushing is fixed at No. 9.

Upon selecting column flushing, program No. 9 is
automatically set for flushing.
When column flushing is set, program No. 9 is not
usable as a gradient program. But program No. 9 is
usable with a link program.
The pump is stopped automatically when program
No. 9 ends.
4 - 28

Following is the relation between delay time and column flush.
Delay time Column flush
Gradient end
(1) With AUTO STOP at YES
(a) When YES is selected for COLUMN FLUSH

Analysis 1 Analysis 2 Analysis 3 Analysis n∗
Delay time COLUMN FLUSH
Stabilizing time
Pump stop
∗ “n” indicates number of analyses specified with autosampler, etc.
Stabilizing time is the time from the end of one analysis until the start of the
next analysis.
Column flushing starts when delay time has ended.
(b) When NO is selected for COLUMN FLUSH

Analysis 1 Analysis 2 Analysis 3 Analysis n∗ Pump stop
Delay time
Stabilizing time
Stabilizing time is the time from the end of one analysis until the start of the
next analysis. The pump stops when delay time has ended.
(2) With AUTO STOP at NO

Analysis 1 Analysis 2 Analysis 3 Analysis n∗
Pump remains in
liquid delivery status.
Stabilizing time
The pump remains in liquid delivery status.
4 - 29

4.3.8 Event Signal Code
The event should be executed manually. The event function works upon input of an event
code. It is used for starting an external device, for stopping the pump, for sounding the
buzzer, etc.
Also, if a pressure limiter error occurs, a signal can be output at event code No. 4.
Monitor screen
UTLTY

Select EVENT.
EVENT SET: PRESS LIMIT=1, EVENT CODE=2, [--]

1
[PRESS LIMIT]
PRESS LIMIT: NONE=0, PULSE=1, LEVEL=2 [--]
<0-2> 1 (EVENT NO. 4)
Enter the desired code. If a pressure limit error is

detected, an event signal is output at EVENT No. 4.
Monitor screen
SET EVENT CODE: [--]

<0-92>
Input event code.
Monitor screen
4 - 30

Event codes and related operations are explained below.
Control Item Event Code Remarks

Timer 1 10 11 12 1st digit:
Timer no.
2 20 21 22 2nd digit:
Action pattern
3 30 31 32
4 40 41 42
Start
Action Contact point ∗ Contact closes for 1 sec.
opens. Open
Signal Contact 1 s
turns closes.
off.
Signal Close
Start turns on. marker ∗
point
Pump 00 Pump stops

Buzzer 92 Buzzer sounds for 5 sec
4 - 31

4.3.9 Error In Signal
Monitor screen
UTLTY

Select OTHERS.
OTHERS ERROR IN=1 LEAK SENSOR =2

1
Select ERROR IN.
ERROR IN SIGNAL DISABLE=0 PUMP OFF=1

1
DISABLE: The pump is not used ERROR signal to stop the unit in e-Line
PUMP OFF: The pump stops the flow when it received the ERROR signal
in e-Line
Monitor screen
4 - 32

4.3.10 Leak Sensor
Monitor screen
UTLTY

Select OTHERS.
OTHERS ERROR IN=1 LEAK SENSOR =2

2
Select LEAK SENSOR.
LEAK SENSOR OFF=0 ON=1

0
Select “ON” for using the leak sensor or “OFF” for avoiding its
use
Monitor screen
4 - 33

4.4 Setting of GLP Functions
Explained here are the confidence functions such as logbook display and resetteng.
4.4.1 Logbook
Logbook can be displayed or reset.
(1) Logbook Display
Logbook can be displayed in the following procedure.
Monitor screen
GLP
GLP: LOGBOOK=1, PRESS M.=2, [--]

1 KEY LOCK=3
Select LOGBOOK
LOG BOOK: REPORT=1, RESET=2, [--]

1
Select REPORT for displaying logbook or RESET for resetting the logbook.
REOPORT LOGBOOK/REPORT [--]

SOLVENT=99999 1 (2 25 2002)
Total flow after replacement of the pump seal will be

displayed. Pump seal replacement date (month, day,
year) is indicated in parentheses.
ESC
Monitor screen
4 - 34

RESET LOGBOOK/RESET: [--]
(MM, DD, YYYY) 2 25 2002
Logbook is reset.
NOTICE: Input the date of pump seal replacement.

Input is allowed in the order of month (M),
day (D) and year (Y).
Upon resetting, total flow value is reduced to
0 and the integration of flow starts. Unless
the ENTER key is pressed after entry of
all date values, resetting does not become
effective.
Even when the year is the same as the
displayed one, you should enter all values
and then press the ENTER key.
Monitor screen
4 - 35

4.4.2 Pressure Monitor
The number of double-speed section errors in each measurement can be indicated.

A double-speed section error indicates that the pressure did not return to the reference level
(previous pressure) even after double-speed control (an increase of pump motor speed) was
effected to compensate for a pressure drop. This may be caused by formation of air
bubbles in the flow path.
Monitor screen
GLP`
GLP: LOGBOOK=1, PRESSURE M.=2, [--]

2 KEY LOCK=
Select PRESSURE M.
PRESSURE MONITOR [--]

9999 TIMES
Display the number of double-speed section errors.
ESC
Monitor screen
Resetting occurs on the following occasions.

• On input of [PUMP ON] or [PUMP OFF]
• On activation of contact
• On start of gradient elution
• On reception of start signal from integrator in D-line configuration
• On external start of pump
• On input of [PURGE ON] or [PURGE OFF]
4 - 36

3.8.3
4.4.3 Key Lock
Keyboard keys can be locked.
Monitor screen
GLP
GLP: LOGBOOK=1, PRESSURE M.=2, [--]

3 KEY LOCK=3
Select KEY LOCK.
KEY LOCK: YES=1, NO=0

1
Locks the keys on the operation panel in order to prevent undesired key entries.
Monitor screen
(LOCK OFF)
ESC
Key lock can be released by the [ ESCAPE (LOCK OFF) ] key.
Monitor screen
4 - 37

5. MAINTENANCE
5.1 Checking the Performance and Specifications
Performance checking method is explained here.
5.1.1 Items to be The following items are required for performance check.
Prepared for
Performance (1) Component Units of Liquid Chromatography System
Check
• Pump (Hitachi L-2100/2130 or equivalent)
• Detector (Hitachi L-2400 or equivalent)
• Data processing unit
• Waste liquid bottle
NOTE: If deaeration of detergent is inadequate, full

performance may not be provided. In this
case, deaerate the detergent sufficiently of
connect it through a degasser.
(2) Items for Performance Check
Prepare the following items for performance check.
• Load resistance coil

A coiled stainless tube with an inner diameter of
0.25 mm and a length of 10 m
• 60% methanol in about 1000 mL
A mixture of methanol 60% and distilled water 40% in
volume. This should be prepared separately.
• Distilled water in about 500 mL
(3) Performance Check Parts Attached to Model L-2200

Autosampler
• Standard sample
60% methanol solution of methylparaben
(Methylparaben 6 mg in methanol 60 mL + distilled
water 40 mL)
• Sample vial, cap, septum
• Standard rack
5-1

5.1 Checking the Performance and Specifications
5.1.2 Preparing the (1) Figure 5-1 shows how to connect the units for performance
System for check. According to this figure, carry out tubing.
Operation Before energizing the system, ensure that the tubing
between the autosampler and pump/detector is properly
connected.
Detector (wavelength 250 nm, time constant 0.1 s)
Waste liquid
bottle
Load resistance coil
stainless pipe 0.1 mm i.d. × 10 m long(L-2100)
stainless pipe 0.25 mm i.d. × 10 m long(L-2130)
Autosampler
(Injection valve)
(Syringe valve)
Pump
Waste liquid
bottle
Eluent Detergent
Fig. 5-1 Flow Path Diagram
(2) Connect the e-Line cable among the autosampler, detector

and pump.
(3) Check if the drain tube is properly connected to the waste

liquid bottle. The waste liquid bottle must be at a lower
position than the autosampler.
5-2

5.1.2
(4) Prepare other units to be connected to the autosampler as

described in the respective instruction manuals.
(5) Put the drain tube from the detector into the exclusive
detector waste liquid bottle. If this tube is put into other
waste liquid bottle such as for the Model L-2200
autosampler, baseline fluctuation may be caused due to
unstable back pressure.
(6) After confirmation of the above steps, connect the eluent to

the pump, and the detergent to the autosampler. Be sure
to use the eluent and detergent of the same production lot.
(7) Start the pump to fill each flow path in the pump and
autosampler with liquid.
(8) After the eluent has reached the injection valve of

autosampler, turn on the autosampler power supply. If the
injection valve is switched over when no liquid is [INITIAL],
the valve seal may be damaged. To prevent this, be sure
to start the pump earlier.
The autosampler will go through the [INITIAL]ialization
sequence, and then the [INITIAL]ial screen will appear.
5-3

(11) Set the pump flow rate to 0.2mL/min L-2100 .
Set the pump flow rate to 1.0mL/min L-2130 .
(12) Set the detector wavelength to 250 nm. Set the detector
time constant to 0.1 s.
(13) Set the following conditions in the data processing unit.
SAMPLE PERIOD : 100 ms

STOP TIME : 3 min
5.1.3 Checking the Check the reproducibility of the injection volume in the following
Flow rate procedure.
stability
(1) Press the PROG key and set the following conditions.

0.0 1.000 1
Setting can be completed by pressing the ESC key.
(2) Prepare 60% methanol for the eluent and detergent.

Wait until the pump, detector, etc. are warmed up and the
baseline stabilizes.
(3) Place a vial containing at least 1 mL of standard sample in

rack position No. 1 and set the rack in the autosampler.
(5) Press the START key of autosampler to start analysis.

The analysis is carried out 6 times. The operating
conditions during analysis are indicated on the display.
Ensure beforehand that the room temperature will not
change suddenly during analysis.
(6) Data Check
Determine the coefficient of variation (RSD) of peak area

by the following calculations.
n
∑ xi
i =1
Mean value x=
n
n
∑ ( xi − x )
2
i =1
Standard deviation SD =
n −1
5-4

SD
RSD of variation RSD = × 100(%)
x
xi: Area value due to each injection,

n: Number of injections
Make sure the RSD value is 0.075% or less. However,

this value represents the reproducibility of not the pump
alone but the entire chromatographic system.
Therefore, the value varies with each stability of the
autosampler and detector, accuracy of the data processing
unit and its processing parameters.
5-5

5.2 Periodic Maintenance
Perform period maintenance and inspection according to Table 5-1. This prevents
problems and ensures that your system meets specifications.
Table 5-1 Period Maintenance and Inspection
Check Location Procedure Remarks

1. Small particles Wash the solvent filter Intrusion of particles
(dust) are often with an ultrasonic cleaner into the pump causes
present in the every 4 months. increase and/or unstable
solvents. Clean the solvent bottle pressure and flow and
every 2 days. composition problems will
Ensure that you are using occur.
the correct solvent when
Solvent refilling the solvent
filter bottle.
2. The column is 1) Do not stop the pump • Part of a complex

expensive. just after completion sample is apt to remain
of an analysis! in the column.
The pump must be kept This not only causes
running for 30 poor stability or
minutes after drift, but shortens the
completion of an column life.
analysis. • Particles present in
2) Do not introduce a the sample plug the
sample containing column filters.
particles! Pressure will increases
Ensure that the sample abnormally. Impurities
contains no particles in the sample also
or impurities. contaminate the column.
3) Periodically clean Impurities cause
the column! erroneous data.
Components left in the
column will contaminate • Occasionally clean the
it. column by injecting a
large amount (1 to
3 mL) of solvent.
The injection of the
solvent will dissolve
the sample residues.
Cleaning of the column
insures good separation
and stability.
5-6

Check Location Procedure Remarks
3. The flow path Wash the filters with an The filters prevent
filter of the drain ultrasonic cleaner every wornout fragments of the
valve is easily 4 months. pump seal from flowing
contaminated. into the column. If the
filter is contaminated,
the pressure increases
abnormally.
4. The pump is the 1) The pump seal is a • Deterioration of the

main component of a consumable part! seal causes leaks.
high speed liquid Be sure to replace Leaks lead to poor
chromatograph. the seals baseline stability and
periodically. data reproducibility.
2) The high pressure The net result of leaks
pump must be kept is degradation of
clean! reliability in
Clean the pump every measurement.
month. • Contamination of the
For cleaning, just check valve, which is
pass isopropanol the critical part in a
through the pump for high pressure pump,
30 minutes. causes pressure
3) The high pressure fluctuations, a failure
pump does not function in the pressure rise or
properly when easy adhesion of air
there are bubbles. bubbles.
Before exchanging the
solvent bottle or • Air bubbles in the pump
adding solvent into cause pressure to
the bottle, be sure fluctuate or fail to
to turn off the pump. rise.
5-7

5.4 Troubleshooting
NOTICE: If the pump should become faulty, contact the dealer

from whom you purchased the pump. Repair must be left
to personnel who have undergone Hitachi High-
Technologies Corporation technical training since
potential hazards are involved.
5.4.1 Error Messages and Proper Measures
WARNING
Beware of Electric Shock!
Before removing the pump cover for parts replacement, etc., make
sure to turn OFF the power switch and disconnect the power cord
from the receptacle.
(1) If an abnormality occurs on the Model L-2130 pump, an

error message appears on the LCD (Liquid Crystal Display).
Error displays result from either the detection of a fault
during the self-diagnostics at power up (upon
[INITIAL]ialization), or from a problem which occurs
during operation.
Fig. 5-1 shows error detecting functions and the error

messages.
5-8

Operation Function Error message
Power ON [INITIAL]ialization
ROM ERROR
Error YES RAM ERROR
generating EPROM ERROR
condition MOTOR [INITIAL]IALIZE
ERROR
E-LINE ERROR
NO
Power ON Pump operation
Error PRESSURE MAX LIMIT ERROR

YES
generating PRESSURE MIN LIMIT ERROR
condition EXTERNAL STOP!
NO
Fig. 5-1 Error Generation and Error Messages
5-9

7.1
(2) Each unit [INITIAL]ializes immediately after its power is

turned on. If a problem occurs, an error message displays
in the LCD. If a problem develops during an analysis, an
error message will also appear in the LCD. Use the CL
key to erase the error messages. Unless the cause of an
error is eliminated, the same error message will display
again.
For units other than the Model L-2100/2130, check the
corresponding instruction manuals.
Table 5-2 Model L-2130 Error Messages
No. Screen Display Contents of Error Measure

1 e-LINE ERROR Error in This error can be cleared
communication via temporarily through the CL
the e-line key. Reconnection is not
possible until you turn the
power on in both the master
and the slave stations.
2 ROM ERROR Contents of ROM are Turn the power off and then on
abnormal. again. If the same error
occurs, notify the service
personnel.
3 RAM ERROR The result of RAM Turn the power off and then on
check is abnormal. again. If the same error
personnel.
4 EPROM ERROR Contents of EPROM Turn the power off and then on
are abnormal. again. If the same error
personnel.
5 PRESSURE MAX LIMIT Upper pressure limit Check the flow for leaks or
ERROR exceeded (∗1) clogging.
6 PRESSURE MIN LIMIT Lower pressure limit Check the flow for leaks or
ERROR exceeded clogging.
7 MOTOR Motor rotation is Turn the power off and then on
[INITIAL]IALIZE abnormal. again. If the same error
ERROR occurs, notify the service
personnel.
8 EXTERNAL STOP! The pump is stopped To clear the error message,
by the emergency press the CL key.
stop contact of
E-Line.
(∗1): PRESSURE MAX LIMIT ERROR also appears when the pressure exceed the
internal pressure limit. The limit is changed automatically related
to the flow rate. The internal pressure limit is 20 MPa for 0 to 15
mL/min, 15 MPa for 15.01 to 25 mL/min, and 12 MPa for 25.01 to 30
mL/min.
5 - 10

5.4.2 Troubleshooting
Table 5-3 offers a guideline for the troubleshooting of the pump. Refer to this table
first, if a problem occurs.
Table 5-3 Model L-2100/2130 Troubleshooting
No. Symptom Cause Measure

1 Input mode cannot be The monitor screen is Set the programming mode
set by pressing not in the programming by pressing the
PROG key. mode. [INITIAL] key.
2 Pump does not start Flow is set at 0. Set the flow through the
when pressing the [PROG] of the MANUAL
PUMP ON/OFF key. function.
3 Gradient delivery 1) Display is other 1) Call the monitor
does not start when than the monitor screen by pressing
pressing the screen. the ESCAPE key.
START/STOP key. 2) The programming mode 2) Set the programming
is not set. mode by pressing the
[INITIAL] key.
4 Purging cannot be set Display is other than Call the monitor screen
or canceled by the monitor screen. by pressing the ESCAPE
pressing the PURGE Numerical input is key.
key. awaited.
5 After appearance of 1) The E-Line cable is 1) Connect the E-Line
the Initialization not connected. cable.
screen when the power 2) In the E-Line 2) Turn on the E-Line
switch has been configuration, the setting of the other
turned on, the E-Line other unit connected unit.
connection wait is not energized.
screen remains on the
screen. The display
does not change over
to the monitor
screen.
NOTICE: The whirring sound of the pump during operation varies

depending on such operating conditions as flow rate
and pressure. Although the whirring sound may become
larger anywhere in the high-pressure range, it is not
a symptom of abnormality.
5 - 11

6. PARTS REPLACEMENT
6.1 Consumables and Spare Parts

The following table lists part numbers, names, shapes, specifications, etc. of replacement
parts. These parts are necessary to keep the system in optimum operation condition. Use
this when ordering parts. Normal life indicates the lifetime of the part when supplying
methanol at a rate of 1.0 mL/min and pressure of 19.6 MPa.
Normal
Part No. Name Shape and Specifications Position Used
Life
890-1130 Seal for L- Pump head 640h
2100
655-1080 Seal for L- Pump head 640h

2130
885-2920 Seal Drain valve 1920h
890-1128 Plunger for Pump head —

L-2100
890-1125 Plunger for Pump head —

L-2130
890-1206 Check valve Pump head —

(on discharge If check valve
valve) for L- doesn't work
2100
properly, clean.
If this doesn't
solve the
problem, replace
890-1205 Check valve the valve.
(on suction
valve) for L-
2100
6-1
25 mm

810-1005 Check valve Pump head —
(on discharge Discharge valve Suction valve
Packing (B) If check valve
valve) for L- 810-1344 doesn't work
2130 properly, clean.
810-1004 Check valve Check valve If this doesn't
810-1325 solve the
(on suction
valve) for L- problem, replace
Packing (A)
2130 810-1345 the valve.
Check valve
810-1325
Packing (A)
880-1345
Valve holder
25 mm
810-1031 Flow path Filter
Drain valve 1920h
filter 638-1423
Packing
638-1422
810-1128 Packing Drain valve 1000 L
6.2 Flow Path Tubing Parts

L329235 SUS push screw M6 1/16 inch (1.57 mm)
(M6) OD SUS pipe
11
655-1458 SUS push screw S No. 10 32 UNF 1/16 inch (1.57 mm)
OD SUS pipe,
Rheodyne injector
9.6
655-1459 SUS push screw M No. 10 32 UNF 1/16 inch (1.57 mm)
OD SUS pipe,
Rheodyne injector
13.5
6-2

810-1310 PCTFE push screw M8 3 mm OD PTFE tube
(3.2 ID)
13.5
810-1311 PCTFE push screw M6 2 mm OD PTFE tube

(2.2 ID)
14
810-1312 PCTFE push screw No. 10 32 UNF 2 mm OD PTFE tube

(2.1 ID)
10.5
638-1099 SUS ferrule 1/16 inch OD SUS

(SUS 316) pipe
1/16 inch
3.9
L369053 PCTFE ferrule 1/16 inch PTFE tube

1/16 inch
3.9
F275108 PTFE tube 0.25 mm ID × 1.57 mm OD × Inlet tube for UV

10 m detector
6-3

(cont’d)
F275144 PTFE tube 0.33 mm ID × 1.57 mm OD × • Outlet tube for
10 m UV detector
• UV detector back
pressure tube for
high pressure
gradient elution
• Reaction coil for
reaction LC
F275112 PTFE tube 0.5 mm ID × 1.5 mm OD × 10 m • For tubing
connection with
reaction pump
• Inlet/outlet tube
for collecting
cell
F275114 PTFE tube 1.0 mm ID × 2.0 mm OD × 10 m Connecting tube
between
proportioning valve
and pump
638-1140 PTFE tube 1.5 mm ID × 3.0 mm OD × 10 m Solvent suction
tube for pump
634-1976 Stainless steel 0.25 mm ID × 1.57 mm OD × • Pipe for high
pipe 5 m pressure gradient
elution
• Pipe for between
injector and
column
634-1977 Stainless steel 0.5 mm ID × 1.57 mm OD × Inlet/outlet pipe
pipe 10 m for dynamic mixer
638-2185 Stainless steel 0.8 mm ID × 1.57 mm OD × 4 m Internal pipe in
pipe pump, drain pipe
633-1263 Nipple (made of M6 M6 1/16 - 1/16 pipe
SUS) connection
28
885-1602 Nipple (made of No. 10 No. 10 Same as above

SUS) 32 UNF 32 UNF
21.5
6-4

(cont’d)
885-1601 Nipple (made of M6 No. 10 Same as above
SUS) 32 UNF
24
885-2179 Nipple (made of No. 10 No. 10 Same as above

Daiflon) 32 UNF 32 UNF
21.5
6-5


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Hitachi L-2100

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INSTRUCTION MANUAL

MODEL L-2100 PUMP

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ABOUT THIS MANUAL

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1. Possible Electromagnetic Wave Interference Caused by This Instrument

Where this instrument is used in a residential area or an

2. Possible Electromagnetic Wave Interference Affecting This Instrument

Where this instrument is used near an intense electromagnetic

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The Model L-2100 pump is warranted to be free from defects in

(1) Scope of Warranty

Any parts which prove to be defective in design or

(2) Warranty Period

One year from the date of initial installation

(3) Availability of Technical Support Service

Technical support service for this instrument is available

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(a) Failure due to operation at a place not meeting the

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(5) Disclaimer of Warranty

THE MANUFACTURER MAKES NO WARRANTIES,

(6) Warranty Card

The warranty card will not be reissued. Keep it in a safe

Installation, Relocation and After-sale Technical Service

Installation of the instrument shall be carried out by or under

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For the customers to acquire in-depth understanding of the

1. Handling of Chemicals and Samples

(1) The user is responsible for following relevant legal

(2) Reagents, standard solutions and accuracy-control

2. Notice on This Instruction Manual

(1) The information contained in this manual is subject to

(2) This manual is copyrighted by Hitachi High-Technologies

(3) No part of this manual may be reproduced or transmitted in

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The liquid chromatograph system consists of a pump for feeding

1.1 Isocratic System

The isocratic system has a simple configuration in which single-

Washing solution Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

Model L-2100/2130 pump

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(1) An eluent is fed by the pump through the degasser.

(2) A sample is injected from the autosampler.

(3) The injected sample is separated through the column which

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1.2 Low-Pressure Gradient System

In the low-pressure gradient system, two or more solvents are

Washing solution Eluent

System power supply unit

Model L-2400 UV detector

Model L-2300 column oven

Model L-2200 autosampler

(2) In the low-pressure unit, the eluents are mixed at a pre-

(3) Through the mixer, the mixture eluent is delivered.

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column which

(6) Each component separated from the sample is detected as

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