Name: Kelompok 3D

Exercise 2: Skeletal Muscle Physiology: Activity 6: The Skeletal Muscle Length-Tension Relationship Lab Report

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1. During an isometric contraction

You correctly answered: d. the skeletal muscle is generating force, but it remains at a fixed length.

2. The force that results from muscles being stretched is

You correctly answered: a. passive force.

3. Active force
You correctly answered: b. is determined by the amount of myosin bound to actin.

4. When you generate the isometric length-tension curve, which of the following forces will not be indicated on
your screen?
You correctly answered: c. tetanic force

5. Passive force in skeletal muscle is largely caused by

You correctly answered: c. the protein titin.

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Experiment Results
Predict Question:
Predict Question: As the resting length of the muscle is changed, what will happen to the amount of total force the
muscle generates during the stimulated twitch?
Your answer : c. Total force can increase or decrease depending upon the starting resting length.

Stop & Think Questions:

Note the dip in total force at a muscle length of
90 mm as compared to the total force at a muscle length of 80 and 100 mm. Why does this occur?
You correctly answered: d. At this muscle length, active force has decreased in value and passive force has not
yet increased to a significant value.

Experiment Data:

Voltage Length Active Force Passive Force Total Force

8.5 75 1.82 0.0 1.82
8.5 50 0.11 0.0 0.11
8.5 50 0.11 0.0 0.11
8.5 80 1.75 0.02 1.77

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Post-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly.

1. When a skeletal muscle is stimulated and generates force but remains at a fixed length
You correctly answered: d. the muscle is contracting isometrically.

2. Which protein is mostly responsible for the development of passive force in a

muscle? You correctly answered: d. titin

3. In skeletal muscle, active force stimulated through a range of muscle lengths

You correctly answered: b. will utilize ATP hydrolysis to drive the cross bridge cycle.

4. Which of the following is not depicted in a typical skeletal muscle isometric length-tension
curve? You correctly answered: a. time

5. Maximal active tension will be produced in a skeletal muscle fiber when

You correctly answered: a. the fiber is at its resting length.

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Review Sheet Results
1. What happens to the amount of total force the muscle generates during the stimulated twitch? How well did the
results compare with your prediction?
Your answer:
Jumlah usaha yang dikeluarkan oleh otot dipengaruhi oleh waktu istirahat otot mula-mula. Baik pada pemanjangan
maupun pemendekan otot, keduanya akan mengubah jumlah usaha yang dihasilkan oleh otot. Jika otot diperpanjang
maka aktivitas otot pasif meningkat, sementara jika otot diperpendek maka aktivitas otot aktif meningkat. Oleh karena itu,
sesuai dengan hasil prediksi, dengan adanya usaha dari otot aktif dan pasif, total usaha akan berubah naik atau turun
tergantung pada panjang otot mula-mula.

Sumber: Moo EK, Leonard TR, Herzog W. The sarcomere force–length relationship in an intact muscle–tendon unit.
Journal of Experimental Biology. 2020 Mar 15;223(6):jeb215020.

2. What is the key variable in an isometric contraction of a skeletal

muscle? Your answer:
Variabel kumci pada kontraksi otot isometrik adalah panjang otot. Pada kontraksi isometrik, panjang otot tidak
mengalami perubahan.

Sumber: Gash MC, Kandle PF, Murray IV, et al. Physiology, Muscle Contraction. Treasure Island (FL): StatPearls
Publishing; 2023.

3. Based on the unique arrangement of myosin and actin in skeletal muscle sarcomeres, explain why active force
varies with changes in the muscle's resting length.
Your answer:
Gaya aktif dihasilkan dari filamen tebal miosin yang berikatan dengan filamen aktin tipis, mengaktifkan siklus jembatan
silang dan hidrolisis ATP. Usaha otot aktif bergantung pada panjang istirahat otot. Ketika panjang istirahat otot
diperpendek, jumlah usaha aktif meningkat. Ketika panjang istirahat otot diperpanjang, jumlah usaha aktif berkurang.
Perubahan besaran usaha ini tergantung pada jumlah miosin yang terikat pada aktin. Semakin pendek panjang istirahat
otot, semakin banyak filamen miosis yang berikatan dengan filamen aktin.

4. What skeletal muscle lengths generated passive force? (Provide a

range.) Your answer:
80, 90, dan 100

5. If you were curling a 7-kg dumbbell, when would your bicep muscles be contracting
isometrically? Your answer:
Saat lengan diangkat ke atas dari posisi lurus ke bawah dan juga saat lengan dibalikkan ke posisi ke bawah dari
posisi lurus.

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