Book of

abStracts
17TH INTERNATIONAL
SYMPOSIUM OF
BIOLOGY AND
ANIMAL NUTRITION

September 29th 2023, INCDBNA Balotesti, Romania

17th International Symposium of Biology and Animal

Nutrition

BOOK OF ABSTRACTS

September 29th 2023

INCDBNA IBNA Balotesti, Romania
45
17th International Symposium of Animal Biology and Nutrition

Optimization of Probiotic Viability via Freeze-Dried Encapsulation with different

cryoprotectants
Dan Rambu*, Mihaela Dumitru, Georgeta Ciurescu
Laboratory of Animal Nutrition and Biotechnology, National Research Development Institute for
Biology and Animal Nutrition, 077015 Balotești, Romania
*Corresponding email: dan.rambu@ibna.ro
In order to exert their beneficial physiological effect and to ensure a site-specific release, probiotics
must withstand a wide spectrum of extrinsic factors that include manufacturing, processing and
host organism specific conditions. The aim of this study was to investigate capacity of four distinct
encapsulation materials: skim-milk (SM), maltodextrin (MD), trehalose (THR) and glucose (GLC)
combined with probiotics in a 1:2 ratio (w/w) to serve a dual function by acting as a cryoprotectant
and as a delivery form in order to confer protection against host-related factors such as low pH and
bile salts concentrations (0.3 and 0.5%). After freeze-drying process, the viability of the isolated
strains (Lactobacillus paracasei ssp. paracasei IBNA 01 and Lactobacillus acidophilus IBNA 05)
were higher vs. control for SM, MD, and THR, SM ensuring almost 100% viability for IBNA 01
and 88% for IBNA 05, highlighting the importance of bacterial different phenotypes that impacts
survivability to different conditions. GLC shows no relevant effect on viability for first stain, in
case of second being detrimental. In both strains, the utilization of MD material resulted in
exclusive survivability during 2h-exposure to simulated gastric acid (pH 3). Initial strain exhibited
a reduction in viability at a rate of approx. 5% per hour, second one demonstrating to be resistant
in acidic condition maintaining its viability almost unchanged from the starting point. Tolerance
to bile salts concentrations of 0.3% and 0.5% were high for both strains encapsulated in MD, the
viability of the first strain remained unchanged, while the second strain exhibited proliferation
initially and then both experienced a 5% decline in viability during the second hour. It can be
concluded that SM confer cryoprotection for bacteria subjected to freeze-drying and MD
demonstrate efficacy across all investigated conditions.

Keywords: probiotics; lactic acid bacteria; viability; freeze-drying;

Acknowledgement: This research was funded by the Romanian Ministry of Agriculture and Rural
Development through project ADER 8.1.7 and Ministry of Research, Innovation, and
Digitalization through Project PN 23-20.04.01.