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April 2022, 2022 (4)
Terrific Broth (TB) Medium
This Article
doi:10.1101/pdb.rec085894
Reagent Quantity Final concentration Cold Spring Harb Protoc 2015.
© 2015 Cold Spring Harbor
Yeast extract 24 g 24 g/L
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Tryptone 20 g 20 g/L
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Glycerol 4 mL 4 mL/L - Article Category
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Phosphate buffer (0.17 M 100 mL 0.017 M KH2PO4,
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KH2PO4, 0.72 M K2HPO4) 0.072 M K2HPO4

Add 900 mL of deionized water to 24 g of yeast extract, 20 g of - Services Free Featured Protocols
tryptone, and 4 mL of glycerol. Shake or stir until the solutes Alert me when this article is
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have dissolved and sterilize by autoclaving for 20 min at 15 psi
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(1.05 kg/cm2). Allow the solution to cool to ∼60°C and add 100 posted
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mL of sterile phosphate buffer. Store TB at room temperature; it
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