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79 Commercial and Industrial Applications o
79 Commercial and Industrial Applications o
J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
ABSTRACT
Microalgae are microscopic photosynthetic organisms that are
Keywords: aquaculture, commercial, industrial , found in both marine and freshwater environments. Microalgae
find uses as food and as live feed in aquaculture for production of
microalgae, pigments. bivalve molluscs, for juvenile stages of abalone, crustaceans and
some fish species and for zooplankton used in aquaculture food
Indira Priyadarshani and Biswajit Rath. Commercial chains. Therapeutic supplements from micro-algae comprise an
important market in which compounds such as β-carotene,
and industrial applications of micro algae – A review. J. astaxanthin, polyunsaturated fatty acid (PUFA) such as DHA and
Algal Biomass Utln. 2012, 3 (4): 89–100 EPA and polysaccharides such as β-glucan dominate The
dominating species of microalgae in commercial production
includes Isochrysis, Chaetoceros, Chlorella, Arthrospira
(Spirulina) and Dunaliella. In the present review it has been
focused on the utilility of microalgae( freshwater,marine and other
such habitats) in commercial and industrial sector to harness the
growing demands of such unexplored natural resources
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
Vitamins A, B1, B6, B12, C, E, biotin, riboflavin, nicotinic acid, pantothenate, folic acid
Other Antimicobial, antifungal, antiviral agents, toxins, aminoacids, proteins, sterols, MAAs
for light protection.
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
TABLE-2: Major microalgae commercialized for human nutrition (Adapted from Pulz and Gross, 2004, Spolaore et al., 2006 and
Hallmann, 2007)
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
TABLE-3: Biomass composition of microalgae expressed on a dry matter basis (Um and Kim, 2009;Sydney et al., 2010)
Strain Protein Carbohydrates Lipid
Anabaena cylindrica 43–56 25–30 4–7
Botryococcus braunii 40 2 33
Chlamydomonas rheinhardii 48 17 21
Chlorella pyrenoidosa 57 26 2
Dunaliella bioculata 49 4 8
Dunaliella salina 57 32 6
Dunaliella tertiolecta 29 14 11
Synechoccus sp. 63 15 11
Tetraselmis maculata 52 15 3
2.2 Microalgae and Cosmetics peelers). Microalgae are also represented in sun protection
The pigment content in microalgae is a specific feature of and hair care products. Typical species that are used for
each species. Its evaluation is essential as an indirect cosmetics are Chondrus crispus, Mastocarpus stellatus,
measure of cell growth, as well as a parameter to check the Ascophyllum nodosum, Alaria esculenta, Spirulina
trophic level of waters. Components of algae are frequently platensis, Nannochloropsis oculata, Chlorella vulgaris and
used in cosmetics as thickening agents, water-binding Dunaliella salina. Microalgae are a polyphyletic and
agents, and antioxidants. Some microalgal species are biochemically diverse assemblage of chlorophyll-a
established in the skin care market, the main ones being containing microorganisms capable of oxygenic
Arthrospira and Chlorella (Stolz and Obermayer, 2005). photosynthesis that are predominantly found in aquatic
Microalgae extracts can be mainly found in face and skin environments with observed high levels of ultraviolet (UV)
care products (e.g., anti-aging cream, refreshing or radiation. Certain microalgae produce organic metabolites,
regenerant care products, emollient and as an anti-irritant in such as sporopollenin, scytonemin and mycosporine-like
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
amino acids, to protect themselves from UV radiation while pass through (Table. 4)
allowing visible radiation involved in photosynthesis to
UV SCREENING MICROALGAE
COMPOUND
Sporopollenin Characium terrestre, Coelastrum microporum, Enallax coelastroides, Scenedesmus sp., Scotiella
chlorelloidea, Scotiellopsis rubescens, and Spongiochloris spongiosa, Dunaliella salina,
Chlorella fusca
Scytonemin Chlorogloeopsis sp., Calothrix sp., Scytonema sp., Rivularia sp., and Nostoc commune Lyngbya
cf. aestuarii Chroococcidiopsis sp., Nostoc punctiforme
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
(EPA, C20:5n-3), docosapentaenoic acid (DPA, C22:5n-3), Dunaliella species, Chlorella species and Spirulina species
and docosahexaenoic acid (DHA, C22:6n-3), have been are three major type that have been used successfully to
shown to be effective in preventing or treating several produced high concentrations of valuable compounds such
diseases including cardiovascular disorders, cancer, type 2 as lipids, protein and pigments (Abe et al., 1999; El-Baz et
diabetes, inflammatory bowel disorders, asthma, arthritis, al., 2002; Abd El-Baky et al., 2002) (Table.5).
kidney and skin disorders, depression and schizophrenia.
TABLE-5: Some High-Value Bioproducts Extracted from Microalgae (Li et al., 2008)
Product group Applications Examples (producer)
Polyunsaturated fatty acids (PUFAs) Food additive, nutraceutics Eicosapentaenoic acid EPA) (Chlorella
minutissima) docosahexaenoic acid (DHA)
(Schizochytrium sp.) Arachidonic acid (AA)
(Parietochlorisincise)
2.5 Microalgae and Biofuel ● finally, they can be processed into a broad spectrum of
Microalgae have long been recognized as potentially good products including biodiesel via trans-esterification, green
sources for biofuel production because of their high oil diesel and gasoline replacements via direct catalytic
content and rapid biomass production. In recent years, use hydrothermal conversion, and catalytic upgrading, and
of microalgae as an alternative biodiesel feedstock has bioethanol via fermentation, methane via anaerobic
gained renewed interest from researchers, entrepreneurs, digestion, heat via combustion, bio-oil and biochar via
and the general public. thermochemical conversion, and high protein animal feed.
Algae offer many potential advantages: There are several ways to convert microalgal
● algae can potentially produce 1 000-4 000 gallon/ acre/yr biomass to energy sources, which can be classified into
significantly higher than soybeans and other oil crops. biochemical conversion, chemical reaction, direct
● they do not compete with traditional agriculture because combustion, and thermochemical conversion (Fig. 2). Thus,
they are not traditional foods and feeds and they can be microalgae can provide feedstock for renewable liquid
cultivated in large open ponds or in closed photobioreactors fuels such as biodiesel and bioethanol. (Table.6 &7)
located on non-arable land
● they can grow in a wide variety of climate and water
conditions; they can utilize and sequester CO2 from many
sources
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
3 INDUSTRIAL APPLICATION use of Blue green algae (BGA), apart from increase in yield
and saving of fertilizer nitrogen, the soil physico-chemical
3.1 Use as Biofertilizer properties also improved.There was gradual build up of
residual soil nitrogen and carbon,improvement in soil p H
Microalgae are employed in agriculture as biofertilizers and and electrical conductivity. The grain quality in terms of
soil conditioners. The majority of cyanobacteria are capable protein content improved. Blue green algae belonging to
of fixing atmospheric nitrogen and are effectively used as genera Nostoc, Anabaena, Tolypothrix and Aulosira fix
biofertilizers. Cyanobacteria play an important role in atmospheric nitrogen and are used as inoculants for paddy
maintenance and build-up of soil fertility, consequently crop grown both under upland and low land conditions.
increasing rice growth and yield as a natural biofertilizer Anabaena in association with water fern Azolla contributes
(Song et al., 2005). The agricultural importance of nitrogen up to 60 kg/ha/season and also enriches soils with
cyanobacteria in rice cultivation is directly related with organic matter. A variety of free-living cyanobacteria are
their ability to fix nitrogen and other positive effects for now identified as efficient components of cyanobacterial
plants and soil. After water, nitrogen is the second limiting biofertilizers. In addition to contributing nitrogen,
factor for plant growth in many fields and deficiency of this cyanobacteria also benefit crop plants by producing various
element is met by fertilizers (Malik et al., 2001). With the growth-promoting substances (Table.8)
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
The algal production technology developed and reported by nutritional importance. The microalgae have a significant
different Algologists is very simple in operation and easy in attraction as natural source of bioactive molecules, because
adaptability by Indian farmers. The technology has got they have the potential to produce bioactive compounds in
potential to provide an additional income from the sale of culture, which are difficult to produce by chemical
algal biofertilizer. In general, there are four methods of synthesis. Both cell extracts and extracts of the growth
algal production have been reported viz, (a) trough or tank media of various unicellular algae (e.g. Chlorella vulgaris,
method, (b) pit method, (c) field method and (d) nursery Chlamydomonas pyrenoidosa) have been proved to have
cum algal production method. The former two methods are antibacterial activity in vitro against both Gram-positive
essentially for individual farmers and latter two are for bulk and Gram-negative bacteria. It has also been reported that a
production on a commercial scale. wide range of in vitro active antifungal activities are
3.2 Use in Pharmaceuticals obtained from extracts of green algae, diatoms and
Algal organisms are rich source of novel and biologically dinoflagellates. Microalgae, such as Ochromonas sp.,
active primary and secondary metabolites. These Prymnesium parvum and a number of blue green algae
metabolites may be potential bioactive compounds of produce toxins that may have potential pharmaceutical
interest in the pharmaceutical industry (Rania and Hala, applications(Borowitzka and Borowitzka, 1992;
2008). The existence of bioactive compounds in algae is to Katircioglu et al., 2006). Various strains of cyanobacteria
be expected due to cooccurrence of these organisms in are known to produce intracellular and extracellular
aquatic natural communities, where an inhibitory metabolites with diverse biological activities such as
interaction occurred between producers and competitors antialgal, antibacterial, antifungal and antiviral activity.
within the same habitat. Microalgae contain numerous Temperature of incubation, pH of the culture medium,
bioactive compounds that can be harnessed for commercial incubation period, medium constituents and light intensity
use. They have emerged as important sources of proteins are the important factors influencing antimicrobial agent
and value added compounds with pharmaceutical and production (Noaman et al., 2004).(Table.9)
Chlorella vulgaris Chlorophyta Biomass, Ascorbic Health food, food supplement, Open ponds, basins,
acid food surrogate glass tube PBR
Dunaliella salina Chlorophyta Carotenoid, β Health food, food supplement, Open pond, lagoons
carotene feed
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
3.3 Use As Aquaculture Feed indirectly to the cultured larval organism. Indirect means of
Microalgae feeds are currently used mainly for the culture providing the algae are through artemia, rotifers, and
of larvae and juvenile shelland finfish, as well as for raising daphnia, which are, in turn, fed to the target larval
the zooplankton required for feeding of juvenile animals organisms. Several companies produce aquaculture feeds
(Chen, 2003). The most frequently used species in using Chlorella and Spirulina, or a mixture thereof. Some
aquaculture are Chlorella, Tetraselmis, Isochrysis, Pavlova, examples of the use of microalgae for aquaculture includes;
Phaeodactylum, Chaetoceros, Nannochloropsis, Microalgae species Hypneacervicornis and Cryptonemia
Skeletonema and Thalassiosira. Mainly the microalgae crenulata particularly rich in protein were tested in shrimp
Spirulina and, to some extent, Chlorella are used in this diets (da Silva et al., 2008). Microalgae such as Dunaliella
domain for many types of animals: cats, dogs, aquarium salina, Haematococcus pluvialis and Spirulina are also
fish, ornamental birds, horses, poultry, cows and breeding used as a source of natural pigments for the culture of
bulls (Spolaore et al., 2006). . Favoured genera of prawns, salmonid fish and ornamental fish. Over the last
microalgae for larval feeds include Chaetoceros, four decades, several hundred microalgae species have
Thalassiosira, Tetraselmis, Isochrysis, and been tested as food, but probably less than twenty have
Nannochloropsis. These organisms are fed directly and/or gained widespread use in aquaculture.(fig.3)
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J. Algal Biomass Utln. 2012, 3 (4): 89–100 Commercial and industrial applications of micro algae – A review
ISSN: 2229- 6905
known to be the most profitable business in the El- Baz, F.K. Aboul-Enein, M.A. El-Baroty, G.S. Youssef,
biotechnological industry. It is a wasteless, ecologically A.M. and Abd El-Baky, H.H. 2002 Accumulation of
pure, energy and resource saving process. antioxidant vitamins in Dunaliella salina. Online J. Biol.
Sci. 2: 220–3.
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ISSN: 2229- 6905
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