Page No.

1 1
Expt.No.
EXPERIMENT-3
DNA DETECTION BX GEL ELECTROPHORESIS

Beteckion o DNA by guldubuaphansi

Paininlo
Electomh@NLAS KELUKS To meuLALnt ofuhaxgeel meleuls umdon iu elutriu jild
Slubsephasiiacasied aut y sing agneL o payausuamsuds gl
Dgakasequs anLAMOKL areus (100-60DA) Huan Hnat a auglasnid
Heu psxL ALK deptmdk ou he cemaustnation.ugaxosHum, agasss.
AASed ts senanali lanqL sised manemeleuuls lks DNA Amd RNA
LAmAuATKaion deptmds on thu six o DNA,RNA aand plakoid melteulo. Hiokt
2n4sLitulane uLatDNA.RNA ax plad xuapuÁHIA Leuth LosatstHokiem dugarnou_qul
h DNAamd RNA MMalhuls axL MAgatiuly sharqecd amd moues tsunand amsde
Lpeukise chanqud)ulhn an eleutnu feld s appucd. thià meuzsant depenels
Daa tha iL,iL, maltculon uueight ol sAuelui auids. Small sixed makcules moue
Jastin Haaus tha laxqL sizad 0ncluls
imilasly.diest upa o plawids au alke Mlauatul by kuis mukhed. dus
allud 2Haidium foemide_iiumd10 duked ttu DNA.t inkncalkas uuitt
DNA. dhuulara,lecahn e DNA OM u dulecked cad isualisad ohaa Puaresce
wwnduu UV iglut

Khauinumuut
Qaunami, DNA
bxomicl,
dqanose g ,boru aid, yá,EDTA, AuOKRAL,dUkzUed suaten, eiaidum
yung
onsepdatmal Hu, eppuntlern tilt minspipattt,uicnoaas Cutsa, maqarti
shiuan
Elhctrephonetu uppakatus uith pou SupplaL
Teacher's Signature
 Page No. 1 2
pt. No.

Aumning ukor Ltha lue AulnA a CemmAasndy_usd sq uis-harati-EDTALTBD

loaleur:
ame s-Quctui-EDTA (TAE)J. au skock solubiens axepuypaed as
(ADmLpH :o
5X TBE 1 lau :8ori akid CATD),uis C54g)amd O.6MEDTA
m) amd 0-5MEDTA
5XTAE1 Gu:Nats basu CA4. a4), QCaial auehir acid C5.11
10 ml.pt -o
EDTA O-6 M,pH &.0,100nal
laicdiua larornids stack seluiedissolse 10 mglwl n usadn, ura m am
uRrkimq uiHa
aluwsiniaada ail amd skaxe at c CalumusuuaN olesss uhla
Hain dye hurausik i wutagemiu)
GXaul-Lsadiund ack (oa6 1 bemaphunsl blus ,0-16L yams, 30/.
(v/y
n o ka uuati.turmatiuly.puspan Caadissq.dyt o 10X Cabmg uuurAAL
dur
4-8ma lessnaaspihumal IuAad iml kull,use 4XLoadiug

PuLdu:
aagnuii stinaer
)SisslaL 0.8 q suose in ACOmlo 1IBE y qeniu heatingm
aganoAz ql.
uiHh aot plat oxy AaAicuesdaa) h i uilL ARAult_a 0./
Ancl tauustratim
nCond tas Caatesaks to adcaad add_hidiusod luxanide to qet
0-5uglm. Jliz the entods prepaly.
aga tani Mal btia Aat _ena sidu ql buou
luhue B tuia amd the
indasert tlas ooal a duuh a uLoy tha 1un gan

Hu aggnOA salubion
DTegd Ha Baiiknussoqu te abub 4-5mmm,pouy
trapptol
metHa roy. Cax sheuld ba talksaa ihak ne oim taudlu smusk u
tasql.Noud, hald his Jen aout 30-4Dunti
seliiede.Aume dhu
Maiy, usAesta Har eommb uláuqel has um
Ianis aad knp u qutay ioaa iu slutuphorwin
tank
N Ceun TBE t a Hae tphak so Haat gl a gt aeka imaaositd hy about

Teacher's Signature
 Page No. 13
Expt. No.

viCasautlg leacl DNA LQMpl wnto shs slata of muhsmsed qel. the saumopluu
nwust b suttiuk at Hu knttew Met.
ix)Comnttar alubio ltad insutho Hnat tha migakiu terminal hsuld
at ta tAnd uhuu Lawnplh na kua loaded
Bun alutrseplh.aruubsat an-l0oN_udit brronoskaunal bls miaratu io ta
atta ud e iu qul
)duwa tuu Jlutte ad amde diacennut taa alouuu laada. Sake sut he agarose

xi)oucrA it iA UV ams-luwminakav. dha sepanadad oememiLe NA As boa

uLAHCSAS nden uy ighk uhica maku ih pausnu pasiti

RLMulk
alloil. swnimg Bar gemei DNA n agaoe , o bamds sat annLan diatir
Lidheut umLA tar md e bu q a puMminut RNA cemtaninatienm is
isualised i DNA is ut fuated uultta RNase inatmunt is inuueplia
RNA AMALA asker an ta DNA kuLALuo lhiang small sizad