Diagnostic test for

poultry baceterial
infection
M afzal
Outlines
• Diagnostic test
• Use of diagnostic test
• Diagnostic test steps
• Culture of bactria
• Identification of bactria
• Isolation of bactria
• Serology test
Diagnostic
test
The
determination of
nature of disease
Diagnostic test types
• Isolation
• Identification
• PCR
• Histopathology
• Serology
Diagnostic test used for
• To determine the treatment.
• To initiate the proper control measures.
• To take the suitable preventive measure.
• To understand the epidemiology.
• To know the disease history.
Diagnosis of bactrial disease
steps
• Clinical sign.
• Labortry examination.
• Culture technique.
• Biochemical reaction.
Culture of bactria
• 1.solid media agar

• Liquid media broth

Blood media
Identification of bactria
1.Morpholog
y
2.Growth
3.Enzyme
4.Antigen
Bactrial
susceptibility
test
• Disk diffusion test
• Inhibition zoan
Serological
test
1. ElLISA
2. Immunofloresent antibody
technique
3. Radio immuno assy
4. Agglutination test
5. Compliment fixation test.
6. Precipitation test
Agglutination
test
• The clumping of particle.
• The reaction of antibody
with the antigen.
• Detction of microbial
antigen by using specific
antibody.
• Clumping reaction occure
quickly and easy to
produce.
ELISA TEST
• Elisa test uses antibodies and
change colour to identify.
• Uses a solid fhase enzime (
EII)to detect the presence an
antigen.
• Antigens from sample attached
to surface.

• specific antibody bind to

antigen.
• The antibody link with enzyme.
• substrate colour change.
Thanks