Kendriya Vidyalaya

N.T.P.C Kaniha

Grade - XI
Chemistr
y
2023-24

TOPIC - Investiga
“DETERMINA tory
TION OF
CAFFEINE IN
Project
TEA
SAMPLE”

Sumbited By –
Sumbited to –
KENDRIYA VIDYLAYA N.T.P.C KANIHA

CERTIFICATE
OF
COMPLETION This is
OF to certify that
CHEMISTRY AMIT
PROJECT- KUMAR
SAHOO,
student of class
XI has
successfully
completed their
Teacher’s Chemistry
Signature Investigatory
Principal Project on the
topic
Signature
"Determination
Of Caffeine In
Tea Samples"
under the
 ACKNO
WLED
GEMEN I
would like Tto
express my
special
thanks of
gratitude to
my teacher
Miss Queen
Priyanka as
well as our
principal Mr
Krishan
Kumar who
gave me the
golden
opportunity
to do this
wonderful
project on the
topic
 CONTENTS

SL No. Topics
1 AIM

2 INTRODUCTION

3 USES OF
CAFFEINE
4 EFFECTS OF CAFFEINE

5 THEORY

6 MATERIALS REQUIRED

7 PROCEDURE

8 OBSERVATION TABLE

9 RESULTS

10 PRECAUTIONS

11 CONCLUSION

12 BIBLIOGRAPHY
 AIM

To determine the amount of

Caffeine present in tea
samples.
 INTRODUCTION
Tea is the most commonly and widely used
soft beverage in the household. It acts as a stimulant
for CNS and skeletal muscles. That is why tea removes
fatigue, tiredness, and headache. It also increases the
capacity of thinking. It is also used for lowering the
body temperature.
The principal constituent of Tea, which is
responsible for all these properties, is the alkaloid-
caffeine. The amount of caffeine in tea leaves varies
from sample to sample.
Caffeine constitute about 3% of the tea’s dry
weight. Tea also contain small amount of
theobromine and theophylline, which are stimulants
and xanthines similar to caffeine.
Studies have found that the caffeine contents
of 1 kg black tea ranged from 22-28 mg while in green
tea ranges from 11 – 22 mg reflecting a significant
difference between the two.
 USES OF CAFFEINE
1. In medicine, it is used to stimulates Central Nervous
System and to increase flow of urine.
2. Because of its stimulating effects, caffeine has been used
to relieve fatigue. But it is dangerous and one may
collapse if not consumes it under certain limit.
3. Caffeine is also used in analgesic tablets, as it is believed
to be a pain reliever. It is also beneficial in migraines.

4. Used to restore mental alertness.

5. In certain dietary supplements used for weight loss,and
in many popular energy drinks.
EFF
ECT
S OF
CAF
FEIN
E
POSITIVE
EFFECTS
1)
C
a
f
f
e
i
n
THERO
Y
The
most
importa
nt
methylat
ed
alkaloid
that
occurs
naturall
y is
caffeine.
 Its
molecu
lar
formul
a is:
C8H10
N4O2
 IUPAC
name
:1,3,7-
trimet
hylxan
thine
 Molec
ular
weight
:194.1
9
MATERIALS REQUIRED

 Green Label Tea

 Yellow Label Tea
 Red Label Tea
 Lead acetate
 Chloroform
 Beakers
 Glass rod
 Filter paper
 Funnel
 Water
 PROCE
 F
DURE
i
r
s
t

o
f

a
l
l
,
5
0

g
r
a
m
s

o
f

t
e
a

l
e
OBSERVATION TABLE
 R
E
S
U
L
T
S

Experimen
tally we
found out
that :-
i. 5
0
g
m
of
R
e
d
L
a
Gra
phi
call
y
plot
ting
vari
ous
tea
sam
ples
in
acc
ord
anc
e
wit
h
the
am
oun
t of
caff
eine
 pre

PRECAUTIONS
1. Chloroform should not be inhaled directly for
a larger period of time.

2. Test tubes and glass apparatus should be

handled with care.

3. Care must be taken which heating the samples.

4. Reading must be taken accurately.

5. There should not be any error in the apparatus.

 CONCLUSION
The content of caffeine varies depending on tea type,
which is directly attributed to their processing and
leaf maturity. White tea, made from the youngest tea
leaves contained the highest caffeine content, and
mate and roasted mate tea, the lowest. The
spectrophotometric micro method proved to be the
best alternative for the determination of caffeine
content, exhibiting the most similar results to the
HPLC analysis. According to the obtained results, all
studied teas exhibited high antioxidant capacity, as
opposed to caffeine, indicating that the contribution of
caffeine to the antioxidant properties of these
beverages is irrelevant.
 BIBLIO
GRAPH
Y the analytical
This project is made by using
data provided by the following reference books
and websites:

 https://www.caffeineinformer.com/caffeine-
content/tea-brewed

 http://www.mayoclinic.org/healthy-
lifestyle/nutrition-and-healthy-eating/in-
depth/caffeine/art-20049372?pg=2

 http://www.theteaspot.com/about-tea.html

 Caffeine : How Caffeine Created The Modern

World by Michael Pollan.

THANK YOU