202) QP Code : 92101 nation 2022-24 (Mareb 2024) Odd Semester End-Term Exami 1 Semester PG, MPL, MPA -IOIT - MODERN PHARMACEUTIC TECHNIQUES M.Pharm. L ANALYTICAL MPH, Maximum Marks 75 Time : Three hours SECTION A - (10 x 2 = 20 marks) Very short type questions (Answer ALL) 1, Chemical shift 2, Base peak 3, Fermi resonance 3C.NMR Hollow cathode lamp 6. Retention time 7. Nitrogen rule Elutropie series Shielding and deshielding 10. Two dissimilarities between flame photometry and AAS. SECTION B - (7x 5 = 35 marks) Short type Questions: (Answer any SEVEN) 11. Spin-Spin coupling and Coupling constant. 12, Write short note on ELISA. 13, Sample handling techniques used in IR spectroscopy. [Turn overui 16 16, ¥. 18, 18, 20. 21 2, Write a note on detectors used in GC. Discuss the theory of fourimotty. Explain X-ray crystallography. Blectrophoresis Potentiometry Tonisation methods weed in unans spectrometty SECTION € - (2 10= 20 marks) Long type questions (Answer any TWO) With a nest diagram, explain the principle and instrumentation of HPLC. Discuss the working principle and instrumentation of different thermal methods of analysis, [Explain the theory of U.V spectroscopy. With a neat schematic diagram explain the construction and working of double beam UV: - ble spestrophotometer. @+7=10) ot he arg tt “Time: 10:00 4m te 1.09 Peer eco ri eee ay en MY AYAN PHARMACY NsrrUn nm ne et ay Inunctomes Ti shy a ucomg “Sheen ASMA. Cursos 1 Wiemann “ 2 Wie tang 3 Oi Con ig tes un 5 Eth Tonic main sens -ANSWEK ANY 2 CWO) ouEsTIons sets Den Dee Epi Das Cen ap dir et 7 Wott nt Oo pt ty sot he Tt fe Sane 9 Wik tse sb gin suction ASIAN MONE outst. 19 Ose SEDO Dn ste on SEEN Distt a Pmainedoieongrcote:sa101 | End Term Examination 2021 (ODD Semester 2020) M.PHARM, . SEMESTER 1 ssubjecti Modern Pharmaceutical Analytical Technique Paper No. MPHMPGIMPLIMPA:101T Date: 22/08/2021 Time: 10:00 am to 1:00 pm Total Three (3) hours? two (02) hours for writing plus One (01) how HIMALAYAN PHARMACY INSTITUTE Majhitar, Fast Sikkim-737136 1 SEMESTER M Pham, I SESSIONAL EXAMINATION, DECEMBER 2022 DEI MACE yt INI (MPH, MPG, MPL, MPA-10, ine: hour ‘Maximum Marks:30 ¥ SECTION=A AgsWER ALL QUESTIONS. 2X5=10 Marks) 1. Petrone transition 2. Principle of electrophoresis 3. Column efficiency 4. Principle of GLC. 5, Principle of moving boundary chromatography SECTION-B ‘OLQUESTIONS 6 Principle, instrumentation and application of capilary electrophoresis Marks) 12. Derivation of Beer's Lambert law. {8 Principle and application of ion exchange chromatography. 9. Write a note on detectors used in UV-Visible spectroscopy sECTION=c gysi FR ANY HONE QUESTION (10x10 Marks) 10. Write instrumentation, working principle and application of HPLC. 11, Write an exhaustive note HPTLC. Page of =. Pull Marks: 78, i (OF download and emall of Answer Seript x10 = 20) 4x7 =35) ks x 2-20) etal, srincipal in details,[greats san Wnd Term Haamtnation 2021 [ODD Bemester 2020) wodern Ph MAMKARM, « SEMBATER t gutieet, Modern Pharmacoutionl Analytical Techntgu Paper Noy UMPAIOIT sate yo 2001 yt ly Papne No MPHUMPGMPLAMPA-t0F jy 10:00 am C0 1100 pm otal The ‘pee (08) Hours FOF WEIN pus One (01) hour for (3) bourne find email of Anawer i Marks: 75 ript SECTION.A anewor all questions, Uniniple of fon exchange clionaaography Shickling effect Theoretical plate Dwinciple of spectroflourimett ag’ tw Principle TGA cehemical shit (2 mark x 10 20) overtone Metastable ions 10. Pleetronie transition, SECTION -B Answer any SEVEN of the following. (5 marks x 7 =35) 11, Radiation source oF IR 12, Bcot's Lambert law and its derivation, 1}, Factors influencing chemical shift and spin-spin coupling, 11, Detector used in UV-Visible spectrophotometer 15, Mass fragmentation and its rule 10, Principle, instrumentation and application of DSC, 17. Ionization methods of Mass Spectrometer 18-Principl, instrumentation and application of Capillary electrophoresis. 19- Instrumentation and working principle potentiometer. SECTION -C ‘Answer any TWO of the following. (10 marks x2 =20) 20. Draw a well abeled diagram of HPILC and expan its working principal in details. 21, Write dui about X-ray erystallography. ; 22. Draw a well labeled diagram of NMR spectrometer and explain its working principal in deta ygsenee Page 2 0!HIMALAYAN PHARMACY INSTITUTE Majhitar, East Sikkim-737136 1 SEMESTER M Pharm, I SESSIONAL EXAMINATION, ODD 2021 ARMAC, LANALYTICA (MPH, MPG, MPL, M. ime: Th hour Maximum Marks: 30 SECTION ~ NsWER ALL QUESTION ae 1, Principle of TGA, 2, Flectronic transition, 3, Guard column, 4, Distinguish between HPLC and HPTLC. SECTION -B SWER ANY 2 (TWO) QUESTIONS (6X2=12 Marks) 5. _ Radiation source of UV-Visible spectrophotometer. 6, _ Instrumentation and working principle of DSC. 7. Write a short note on TLC. 8 Principle and application of lon-exchange chromatography. SECTIO} WER ANY 1(ONE QUESTION 9. (10X1=10 Marks) Draw a well labeled diagram of double-beamed UV-Visible Spectrophotometer; explain its working principal and application in details, 40. Write instrumentation, working principle and application of potentiometer. Pt your answer sheets in the pdf formato bibhas.panditegmail.com ___ The pdf file should be re-named as (Roll ‘Number-Subject Code-Date) format. : For example: 21HMPHO1-MPH101-30122021HIMALAYAN PHARMACY INSTITUTE Majhitar, East Sikkim-737136 arm, II SESSIONAL EXAMINATION, ODD 2021-22 gentsTeRM Phi pERN PHARMACEUTICAL ANALYTICAL TECHNIQUES (MPH, MPG, MPL, MPA-101) ar Maximum Marks: 30 ime nh SECTION L QUESTION. (2X4=8 Marks) NSWERAM 4, What are the different types of gels can be used in electrophoresis? How RIA and ELISA are different from each other? ~_/ 2 . 3, Why buffer solutions are used,in electrophoric separation of biological components? 4 DefinePAGE | // SECTION -B 0) QUESTIONS (6X2=12 Marks) 20We Write a note on moving boundary electrophoresis, a Instrumentation and working principle of paper electrophoresis. Write a short note on RIA. /#” Principle and application of 2D gel electrophoresis. SECTION: ‘ANY (ONE QUESTION (10X1=10 Marks) 5. Define the term immunosorbent assay. Write details about ELISA technique. 10. Write instrumentati i ‘inciy tation, working principle and application of SDS-PAGE electrophoresis, SJ Sebmit your ryeanowe: sheds inthe pt formato bibhas.panditegmal.com fle should be re-named as (Roll Number-Subject Code-Date) format. Forexample: 21HMPHO1-MPH101-03.03.2022rotal No- of printed pages = 3 QP. Code : 92101 odd Semester End Term Examination - 2019 Semester-I Course : M. Pharm MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUE Paper : MPH, MPG, MPL, MPA 101T Full Marks—75 Time — Three hours The figures in the margin indicate full marks for the questions. SECTION — A Very short type questions (answer all): 2x10=20 1. Spin-Spin coupling. 2. Stoke’s shift. 3. Fermi resonance. 5 Base peak and parent ion peak. {Turn overEnd (Odd) Semester Examin AI Phare Samm HIMALAYAN PHARMACY INSTITUTE secthlbae Wark CHL inn. 7971A6 5. FAB. 6. Principle of affinity chromatography, SECTION - C 7. Melafferty rearrangement, ong (ype questions (answer any two): 10*2-20 8. Reason for using TMS as standarg 20. write the principle, instrumentation and application rd in |“ of mass spectrometry. 9. Chromophore. 21, Write the principle, instrumentation and application of column chromatography. 10. lon exchange resin. 0, Write an elaborate note on spectroflurimetry. SECTION - B . Short type questions (answer any seven) ; Sx 11. Factors affecting vibrational frequencies : a ‘Spectroscopy. 12, Principle of orescence and phosphorescen 13, Instrumentation of NMR spectroscopy. 14. Paper electrophoresis. 1S. Principle and instrumentation of DTA, | 16, Detectors used in IR spectroscopy, 17. ic f i\sueeeneee mrs 19. Beer Lambert’s law. 96/92101/MpaT @Q 2101/MPAT @) 50(G) eeeEnd (Odd) Semester Exa M Pharm 16 HIMALAYAN PHARMACY INSTITUTE Maihitar Poet Gitleim-737196 Total No. of printed pages = 3 Odd Semester End Term Examination - 2019 Semester-I Course : M. Pharm MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUE Paper : MPH, MPG, MPL, MPA 101T Full Marks—75 Time — Three hours "The figures in the margin indicate full marks for the questions. SECTION — A ay short type questions (answer all): — 2x10=20 in-Spin coupling. [Tum over2018, | ar code: 92101 HIMALAY, : Majhing NrHARMACY INSTITUTE IPL. MPG, MAQ 1011) , im-737136 1 SEMESTER M Pharm, SESSIONAL EXAMI Full Marks: 78 INAT “: Mee TON, ODD 2019 ie: her Maximum Marks: Qxtny-20 ‘SECTION ~ A, ita uNsWER ALL QUESTION. (2X4-8 Marks) 4, What do you mean by allowed transition and forbidden transition in UV spectroscopy? ‘Waa c= the different types quenching in furoscenes? 4g. How many normal vibration modes are possible for ethane and benzene? ‘Why TMS is used as standard for NMR spectroscopy? SECTION-B (6X2=12 Marks) (5x7-35) pifferent modes of vibration of atoms in polyatomic molecules VIR spectroscopy Principle and instrumentation of fluorescence specr08coPY 20 +. itferent sampling techniques used in IR spectroscopy ski's energy diagram. . served in atomic Principle of NMR spectroscopy SECTION=C (aoxi-10 Marks) ag crystal technique ‘Write down the principle, instrumentation and applications of uv. Visible spectroscopy : . — site down the principle instrumentation and applications of : ome” / (10x2=20) “— (S45) (55) mass 5) tie diagramEnd (Odd) Semester Examination — M, Pharm 1 Modern Pharmaceutical Analytical Tony sees HIMALAYAN PHARMACY INSTITUTE Majhitar, East Si kkim-737136 1SEMESTER M Pharm, SESSIONAL EXAMINATIO! IN, ODD 2019 MPAT ime: 1h hOur Maximum Marks: 30 J QUESTION (2X4=8 Marks) 1, Nitrogen rule 2, Base peak and parent ion peak 43, Principle of affinity chromatography 4. Ion exchange resin SECTION-B ANSWER ANY 2 (TWO) QUESTIONS (6X2=12 Marks) Different types of ionization techniques in mass spectrometry 6. Detector used in Gas chromatography 7. Principle and instrumentation of ion exchange chromatography. 8, Principle and instrumentation of column chromatography. ION -C ANSWER ANY 1(ONE QUESTION (10X1=10 Marks) 9. Write the principle, instrumentation and applications of mass spectrometry 10. Write the principle, instrumentation and applications of HPLC 2018 ical Techniques (MPH, MPL, MPG, QP Code: 92101 MAQ-101T) Full Marks: 75 (2x10)=20 (3x7=35) UR spectroscopy ‘opy ski's energy diagram yserved in atomic 1g orystal technique (10x2=20) (5+5) (545) n mass 5) atic diagramFad (Odd) Semester Examination 2018 eee | M. Pharm 1 Sem Modern Pharmaceutical Anaty ting atl Semester Analdtical Techniques (MPH, MPL, str HAQ-101T) ime: 24 Hours Full Marks: 75 See answer all the Questions 2x10-20 Beer-Lambert lay Antistoke's fluorescence Bathochromie shit Fermi resonance Base Peak and parent ion peak Nitrogen rule Define Quantum yield of Nuon 8. Principle of electrophoresis 8. Chemical shift 10. How will you ascertain the IR spectrum Aspirin and salicylic acid? Sectioy Answer any seven questions (5x7=38) {1 Faumerate the diferent factors that affect vibrational frequeney in I spectroscopy 12 Desrie the principle and aplication of Name ension spectroscopy |S Nai the principle of Nuorescence & phosphorescence with lalonski's energy diagram, M4. Write down different non-spectral absorption.spectroscopy & it's remedy 15, Write a short note on 13C NMR 16, Shielding & deshielding phenomena with suitable examples 17. Write down the principle of NMR spectroscopy 'S. Write down different X-ray methods? Write a short note on rotating erystal technique 19 Deseribe in brief the instrumentation of HPLC imterference observed in atomic Section C Answer any two questions 20.1, Write a short note on factors affecting fluorescence. (545) ji Discuss the instrumentation of spectrofluorimetry. 21..Write the Principle and application of UV-Vis spectroscopy. ($+8) li.Deseribe briefly the instrumentation of UV spectrophotometer. 22.1, Describe in brief different types of ionization techniques used in mass spectroscopy (545) Ui Discuss the instrumentation of mass spectrometer with schematic diagramEnd (Odd) Semester Examination — 2017 | , M. Pharm I Semester Modern Pharmaceutical Anatytical Techniques (MPH MP MPI HIMALAYAN PHAR MACY INSTITU ITE, Majhitar, East Sikkim-737136, {Sem M. Pharm 1 Sessional Examination, (Odd 2018 MPAT (MAQ-t01T) secioy_a eer all the questions: (X44) la ¢ the Amax for the compound (base value 253) 3. Caleulate ex swer any two questions:- Ns in UV-vis spesocopy wit exam ities founda U cons 1 we depnplof fuses & pephoseee wih aon ce i 3 Wie enrnention of aonic asomton secon a {eon ditet nefence obser in om absotion or enon pees iseaaty SECTION -C (x=) (nswer any one questions = scence intensity? |. Writ down the factors affecting Nuorescence ny Wis ecroptaoneter 2. Witedown the principle & instrumentation of UV-ays 6 ANSWER ANY SEVEN QUESTIONS Write 4 6 ANSWER ANY TWO QUESTIONS, (Odd) Semester Examination 2017 arn Pharma M. Pharm 1 Semester Mode recutical Analytical Vechniques (MPHAMIPG MPL MPQ-PG TION) QP Code 92101 je 2s Hours ee Full Marks: 75 WER ALL THE QU (12-20) 1 the followings (chemical shite Nitrogen rule Potentiometry Principle of DTA, ‘Quenching Suoke's shifl Effect of temperature and viscosity on Mluorese Metastable ion Ry value (0, Principle of thermal conductivity detectors ice intensity short notes on. Shielding and de-shielding phenomena in NMR General fragmentation pattern in mass spectrometry Basie principle of NMR spectroscopy Electrophoresis Finger print region and functional group region Interference in flame photometry X-ray diffraction Principle of atomic absorption spectroscopy Detectors used in GC Section —C (2X10-20) Enumerate the principle of any two chromatographic techniques you have studied. Discuss the various column efficiency parameters used in HPLC, (S+5= 10) Describe with the help of a neat sketch the instrumentation of a double beam UV-Visible spectrophotometer. Discuss the different factors affecting vibrational frequency in IR spectroscopyEnd Semester (Monsoon) Examination, Dec 2012 suiert HIMALAYAN PHARMACY INSTITUTE 737136 p/SEMESTER M Pharm (COMMON TO ALL BRANCHES) IISESSIONAL EXAMINATION RMACEUTICAL ANALYTICAL TECHNIQUES. ‘10*20) (MPH, MPL, MPG, MAQ-1011) - Maximum Marks: 15 Majhitar, Fast Sikt m. mes 108" SECTION =A yswi8 1 \SWERAN «ALL QUESTIONS How aldehyde? pefine Overtone igre two sources of radiation of IR spectrophotometer . Course: M. Pharm. Semester: 1 vd wavelength (X44 Marks) Ll y do you differentiate between IR spectra of acid and yiRspectrum? coloured substance init the names of two detectors used in IR spectrophotometer, SECTION -B 1 (710) QUESTIONS short note on column chromatography pijference between GC and HPLC (2X2=4 Marks) 5x6=30) £ Cissily chromatography based on mechanism of separation §. Write down the instrumentation of IR spectrophotometer. SECTION ~C \SWER ANY 11ONE) QUESTION (7X1=7 Marks) is ascertained by IR 1. Wnt the basic principle of vibrational spectroscopy? Enumerate the different factors that affect vibrational frequency in IR spectroscopy? (245) 2 Discuss the basic principle of HPLC. Describe briefly the instrumentation of HPLC? (245) x2=20) Jafferty rearrangement. ince to L-butanol and 2- (10)End Semester (Monsoon) Examination, Dec 2012 — Course: M. Pharm, Semester: 1 Modem Pharmaceutical Analytical Techniques sit Paper mpvor FulltMarks 70 SECTION.A 1 Allthe Questions) a (2a10=20) what do you mean by capacity factor? Express the mathematical relationship between wave number, Caffeine molecules absorb inared tation at 1656¢m" Clalsethe oreteagth | See aaa 36 cm. Calculate the wavelength Whats Eddy diffusion? List out the window materials used in IR, Aspirin is prepared from salicylic acid, How will you ascertain the react ? Listout the type of interferences, “onbyopecun write the method of calculation & significance of coupling constant. 0. Nitro acetanilide is deep yellow but para nto acetanilide Is yellow coloured substance Explain the statement with respect to UV spectroscopy, SECTIONS answer any six Questions) tan Explain the advancement in HPTLC over TLC Explain the shielding and deshielding proton with suitable examples. \write short nates on electronic transition in UV. \write a note on column efficiency parameters, Draw the mass spectrum of phenol and explain its fragmentation patter. Write short notes on cotton effect. fsplain with suitable example how B lactam structure of pencil is ascertained by IR spectroscopy. How will you differentiate the following compounds by 'H NMR spectra? 32} o-Xylene from m-xylene b) Dimethyl ether from acetone c Ethanol from phenol 1 2 3 4 5 6 ‘sectioNn-¢ ‘Answer any two Questions (10*2=20) nitrogen rule and Melfferty rearrangement 1. Explain the basic principle of mass spectroscopy, nce to L-butanol and 2 Describe the fragmentation pattern of mass spectra with special referer buatnol (6+4=10) 2. Classify chromatography based upon mechanism of separation 3. White short notes on the following (4*3#3°10) 4) Explain the basic principle of NMR. ‘Spin -spin coupling ) Coupling constant (10)subiect HIMALAYAN PHARMACY INSTITUT Maj ir, East Sikkim-737136 mM. Phaem 2! Sesona! Examination (044) 2017 sem Mt MPAT (PG-TIO1) Fatt Marks 20 rime: 1 A cexses) wp a " ” coupling Constant re > een Rue Bema Shih ection =» ecto 8) 00 axne “ising and desheldng phenomena ; Fa cemention poner in ase spectrometry 2KD 2 erate of NMR Spectroscopy Bp etc pes ots produced in mss speciromery section _ nor os ne fom tefllowings- a } 1. Discus the principle and instrumentation of mass spectrophotometer with a neat sketch, (C10) 2 Diswsthe diferent factors affecting chemical shift value in NMR. | + JEnd Seren (Monsoon) Examination, Oec 2012 pict ‘Ourse:M. Pharm. Semester: 1 ovor 70 HIMALAYAN PHARMACY INSTITUTE Ss Majhitar, Bast Sikkim-737136 sea M- Pharm 1 Sessional Examination, (Odd) 2017 aa ‘war 5 Full tras 20 imc 1 wavelength. length S aKs5 ssi Ixy ol ecetal re eon : ore hosted tance SECTION -B 6-30) c oy me (3x26 ecu, Ava S of ourimery : aye ips of vibrations in TR Spectroscopy cfkstn UV Spectroscopy S ascertained by IR ce diferent factors that fect vibrational fequency i uency in IR speetrascopy, te insrumentation of a an UV-vis é . ‘double beain UV-visible spectrophotometer with a neat a) afferty rearrangement vee to I-butanol and 2- {10)subiect HIMALAYAN PHARMACY INSTITUTE Majhitar, East Sikkim-737 136 M.Pharm 1 Semester Mid Term Examination (Monsoon) 2013 MPAT (MPY-01) Full Marks: 20 . ur rime: 1 he SECTION-A short Questions. Answer all (1X5=5) xpress the mathematical relationship between wave number, Frequency and wavelength >. aspirin is prepared from salicylic acid. How will you ascertain the reaction by IR spectrum? 3, How many shielded and deshielded proton present in benzene and LB toluene? 4, Explain the McLafferty rearrangement in n-propyl benzene. 5 Derive the Beer- Lambert law equation. SECTION-B Write short notes on (Any two) (3X2=6) 1. Write short notes on electronic transition in UV. 2. Write short notes on spin- spin coupling. 3. Penicillin structure has B lactum ring not oxazolone. justify the statement by IR Spectroscopy. 4. How will you differentiate the following compounds by 'H NMR spectra? ®)chlorobenzene from benzene _b) Benzene from acetone ¢) Ethanol from methanol ECTION. Write long answer on (Any one) (9x1=9) 1 Briain th i ¢ basic principle of -ma: i methemati Iss spectroscopy by deducing the Cee expression m/e a r Describe the fragmentation pattem of mass Special reference to I-butanol and 2-buatnol & tert-butanol 2 by ‘Plain the ingen used nt Principle and application of IR Spectroscopy. Why is mid the structural elucidation rather than near and far IR.iMALAVAN PHARMACY INSTITUTE HUN tajhitar, East Sikkim-737136 «gem M. Pharm, 1" Sessional Examination (Monsoon) 2014 Pharmaceutical Analysis (PHA-PG-107) Full Marks: 20 nes 1 ha rns (UX5=5) nian the folenine Apoicionof FC reagent yi + efeenve stand 3, 10D LOR 4. Analtcl blank {eso Bratton Marshal reagent. SECTION -B weer any ro ofthe following:- axne |. prininteof Turbidometry & Nephelometry. ces benween Fluorescence and Phosphorescence. 5. Principle procedure and uses of MBTH reagent. se involved for quantitative determination of carboxylic acid. SECTION ~C any one fom the followings OX1=9 Desi the principle an ‘trumentation of Flame Photometry with suitable diagram, "principle and procedure involved in various physic-chemical Methods of analysis. wit iazotizati alysis with special emphasis to diazotization and Pou , “us titration of sulphonamide.pie sul HIMALAYAN PHARMACY INSTITUTE, Majhitar, East Sikkim-737136 1 Sem M. Pharm, 2" Sessional Examination (Monsoon) 2014 MPAT 101) , Full Marks: 20 ime: 10 Section — A inthe followings= (1X55) |, Metastable ions 2 NOE 3, Coupling constant 4, Nitrogen s,_ Spin-spin coupling Section - B wate short notes on: ~ (any two) (3X2=6) 1. Modes of fragmentation processes in MS. >, Fundamental principle of NMR. 3, MeLafferty rearrangement. 4, Shielding and deshielding phenomena. Section -C Snsver any one question: — |. Describe in detail the principle and instrumentation of Mass spectrophotometer. 2. Discuss the different factors affecting chemical shift value in NMR.2012 pIMALAYAN PHARMACY INSTITU Majhitar, East Sikkim-737136 nm irsem M:Pharm, 1" Senslonal Examination (Monsoon) 2014 nad Full Marks: 20 4 wavelength ‘length Marks: 29 (UXS=5) vim enero AXSe5) ( spectruny? Coe enon ranston Overs ' otowret sl enc oom comm seme Dassen Hens. waelengh and waive number —-— ( ao mm ( iine Oxo) yeti spectroscopy ox) is ascertained by TRS iterate between IR spectra of ) acetic acid and acetaldehyde ‘aphex and catechol » Shor rae for calculating Jax Of organic compounds Wore ; Dilows es of sibrations in IR spectroscopy SECTION =C sponte followings: @xi=9 (@X1=9) afferty rearrangemen: ike to t-butanol and 2° trophotometer and explain rug. 10 schematic diggram of a double beam UV spect fancions 1 produce UV spectra. tic dtlerent factors affecting vibrational frequency abie HIMALAYAN PHARMACY INSTITUTE, Majhitar, East Sikkim-737136 1""Sem M. Pharm, 2" Sessional Examination (Monsoon) 2014 APPLIED PHARMACEUTICAL ANALYSIS (PHA-PG-107) Fall Marks: 20 rime: 04? SECTION A ower all the questions:- X55) 1, Accuracy >, Ninhydrin reagent 3, Precision 4 Cation -cation interference 5, Robustness & Ruggedness SECTION -B Write Short notes:- (any two) (3x26) |. What is error? Mention the different types of error. 2, Quantitative estimation of Vitamin A. 3, Dumas method for the determination of nitrogen. 4. Quantitative estimation of carboxylic acid. SECTION -C (OX1=9) Answer any one from the followings- tive estimation of any one antipyretic drug. 1. Write a brief note on quant 2. Describe the method validation for HPLC as per ICH guidelines.unio jMALAYAN PHARMACY INSTITUTE, ' Majhitar, East Sikkim-737136 1 | Sessional Examination, (Monsoon) 2018 sem M. Phar ison Nt MPAT (PHA-PG-101) Full Marks: 20 a (IXS-) se atowing EMR in order oftheir increasing energy “ct fllowing 1 nays, Xorays. IR radiation and radiowaves aihclromic shift? se aowing wavelength in wavenumber "nm ss dforem pes of transitions observed in the UY-Visible region of the aoe lemon? C0 pained in UV spectroscopy is broad in nature? wn wats SECTION -B stones on any 100 ofthe following:- (3x2=6) Ties of UT spectroscopy sweefectin UV spectroscopy avs fabsortion isnard Fieser rules for dienes SECTION ‘swoon fram the followings oxi=9 ‘at the diffrent quantitative estimation techniques used in UV spectroscopy an mi instrumentation of a double beam UV-Visible spectrophotometer with a stHIMALAYAN PHARMACY INSTITUTE Majhitar, East Sikkim-737136 Sem M. Pharm 2™ Sessional Examination, (Monsoon) 2015 M.PA.T (PG-101) hour Full Marks: 20 yalthe questions:- (IXS=5) Coupling Constant - Feminsonance ; Importance of fingerprint region in IR Coupled Vibrations NOE SECTION -B short notes on any two:- (x2=6) Principle of NMR Proton exchange reaction Spin-spin coupling Chemical Shift SECTION -c "any one from the followings. OXI=9 Pstss the various factors affecting vibrational frequency in IR Spectroscopy. How do you distinguish between following types of compounds based on IR Spectra 4 Aldehydes & ketones b. Alchols & Carboxylic acids © Amines & amides 4 Aliphatic & aromatic compounds © Carboxylic acids & amides Mpvo1 70 ¥10=20) ! wavelength length IR spectrum? ‘coloured substance 6=30) S ascertained by IR 2-20) ifferty rearrangement. e to L-butanol and 2- to)M. Pharm 1" Semester, End (Mor iar nsoon) Semester Examination 2009 eu Analysis Technique (MPY 01) TON-4 wy the MAS sPeCtTUM OF neo pentane, pe chemical shift coupling Constant? vat ae slents used in NMR. 1 Bragg’ IAW. we og rule, ; pine bs pet and parent ion peak 1. Dig PDA detectors. 4 es do youmean by Asymmetry factor and resolution? 8 weet are the solvents generally used in UV? ra \ * ist 7? ‘ SECTION -B yjardo you mean by chromophore and auxochrome? Ex; cilain them with suitable ‘example. jskeo- enol tautomerism of ethylacetoacetae is stud 4 isd by UV and IR spectroscopy FF yee of signal and approximate chemical shift values of the protons present in the following und. . Ps om a on c=N ooo £ () % Oe (C/OH cH cH, ° ° Cn eS aaa, sg surtnoteson LC-MS. Fao affecting column efficiency. 7 Csi chromatography based on mechanism of separation, { pieenee between GC and HPLC. SECTION -C 2x10=20 A) itedown the wave no of the following groups in IR spectroscopy (5) OF =p of phenol b) NH» group of aniline c) C-H of CHsd) C=O group of acetone CC scp of benzene ring £) C-H of benzene ring g) C-Cl of benzene ring IC ther aromatic ring i) C-H of alkanes j) C-H of alkenes k) C-H of alkynes Alison of Rin qualitative analysis Lip it basic principle, instrumentation and application of PMR spectroscopy. (10) Destin detail the derivatization techniques of GC* End Semester (Monsoon) Examinations, Dec 2011 Course: M.Pharm Semester: unjst: Modern Pharmaceutical Analytical Techniques ‘me: Three Hours ,NSWER ALL THE QUESTIONS Paper: (MPY01) Full marks: 70 (10x2=20) in the followings en Crclar birefringence 2, Precessional frequency 3, Metastable ions 4 Nitrogen rule 5 Finger print region 6. Circular dichroism 7, Circular birefringence §, Metastable ions 9. Resolution 10. Bathochromic shift and hypsochromic shift Section —B ANSWER ANY SIX QUESTIONS Write short notes on: 1. Spin-spin coupling 2. Sample preparation techniques in IR spectroscopy 3. Effect of polar solvents on i) n ~» a* an 4. Gas phase ionization technique 5. Bragg’s law 6 1 8 (6X5 =30) Differentiating an aliphatic aldeh Shielding and deshielding Derive 1yde from an aliphatic ketone in IR spectrum tion techniques used in GC ‘Section—C ANSWER ANY TWO QUESTIONS |. Describe in detail the instrumentation of HPLC with a neat sketch 2. “NMR spectroscopy is the most important tool compared to UV and IR for the structure determination of an unknown organic compound”. Justify this statement, Deseribe in detail the principle and instrumentation of Mass spectrophotometer @X10=20) 3'!* a QP Code $9101 puARMACY 1" SEMESTER, END TERM (MONSOON) EXAMINATION, 2018 MODERN PHARMACEUTICAL ANALYTICAL THCHNIQUES (PHA-PG-101) ys Hoos ‘Maximum Marks : 70 ‘i syeR ALL THE QUESTIONS (10x 2= 20) ste lines Pe Pe lar deboism } onD Fermi resonance spin decoupling Brge’s Law Nitrogen rule specific rotation elaxation process in NMR & an Deemier plot Wi, cued vibration 4 6 8 Section -B ANY SIX QUESTIONS, asm (6X5=30) test nates on 11 Diseuss the different types of detectors used in GC. 12 Blectruphoresis 15, Chemical shift 14, Noodward Fisher rule 15,Charecteristic vibrational frequency for carboxylic acis, amides and aldehyde 16 Laws of absorption of electronic spectroscopy 17 Modes of fragmentation processes in MS 18. Coton effect ANSWER ANY TWO QUESTIONS (2X 10=20) 19. Diflerentiate between a) acetone and acetaldehyde b) Ethyl alcohol and acetic acide) amide and amine d)aleohol and phenol e) ester and ketone 20, Describe in deal the instrumentation of HPLC with aneat diagram 21. Describe with the help ofa neat sketch the instrumentation of @ double beam UV-Visible spectophotometeroo Subject Code: MPY-OF MAHARMACY 1" SEMESTER EXAMINATION JANUARY 2000 MODERN PHARMACEUTICAL ANALYsI5 saws Maxim 70 marks a tH , Section.A, Answer ALL the Questions inte followings (002-20 marks) ase peak in Mass Spectrum Cvomophore and Aunochrome pifference between Document and Records Fermi Resonance Finger Print Region Parton Coefficient > Patent s aaand oc 4, Reference Electrodes in Ptentometry 11 Relationship among Wavelength, Frequency and Wave number Section-B ANSWER ANY SIX QUESTIONS, ite short notes on (625-30 Marks) Chemical shit PAGE Applications of LC-MS Factors relating to quality of Herbal drugs Stationary phases in HPLC Process validation 7. Sampling techniques in IR Absorption laws involved in UV-Visible spectroscopy Section-C ANSWER ANY TWO QUESTIONS (2X10= 20 marks) 1. Explain the salient features of various Quality System elements ofI$0 9000 2 Discuss the instrumentation of UV spectrophotometer and its applications in Pharmacy 4 With a neat block diagram describe the instrumentation and techniques of HPLCCode MPvO1 yl oo PH RMAC uaa AVANT kine 737° anita “caron oton08) 17 ' sen Ten Ext jo esi 70 urna SN qr (MPyOD ‘ruil Marks: 2 mst 0 spCTION:S (x85) samscoasnera 410X220 marks) et we sigue of MS 4 wae abe ‘SECTION: B (2x3=6) pve AE questions:= oe bonderingin CromatoB : E sete cae Fad on mechanism of separation 5. HPLC Column 1, hing effect of | secT1O! (615-30 Masks (9X1=9) gover any ome questi | pin the basic principle of IR. Briefly deseribe the qualitative “plton f IR wth suitable example, writ the approximate wave no of the following Bop, 0H NH yc aow(l 2.4) Electronic transition of UV Spectroscopy. (45) Outline the equation of mle a as) xi 10: 20 marks) ‘Pharmacy rucjon Edge 7 atv and absolute detec 4 i al IR spectroscopy fe . Jar vibrations in {ypesof mol ‘Tye 1 seeamrerting Section -B 2=10) {NER ANY TWO QUESTIONS 6x a on: ; Srl epuatn techniques in TR spectroscopy joodvard-Fischer rule 3 PAGE 4. Cotton Effect Sei (2X10=20) 's about the various Principle and instrumentation of Mass, Stoptotometer Deseribe in detail about the various titati i vious quantita "nse ec a smaion techniquesCode MPY A 001) 2012 sy marks: 20 ae) (4x28) \d validation one question = @xI-8) a! os trarent i aan uta examples appa on of different in drug, eelopment and quality contol 5-30 Masks 2 Goan now aethe data ofa inter-ay precision study - ieee ee 4 3 6 wissy 9833 9864 9789 9897 98.0 97.54 Datei te % RSD and comment whether the inter-day precision io esult comply withthe ICH method validation criteria i 020 marks) Trarmacy ucHIMALAYAN PHARMACY Majhitar, Rangpo, Fast Si 3" Sessional Examination, Fe ‘bruary-2006 M.Pharm First Semester 2005-06 INSTITUTE kRim-737 139 Time: 75 minutes (Answer any five Guestions) explain the basic principle of Bragg’s X-ray spectrophotometer to determine the | structure. crystal spectroscopy. What do you mean by finger print region? Wri boxding for alcohol, phenol, enol, chelates, car Spectroscopy. (5) / Discuss in detail about the instrumentation of a Double focusing Mass Spectrophotometer. (5) wo”. 00 ia IR detail about the molecular vibe 4, isos in i Libis 2255 (eee 2 bat yas Soma e994 1055 onueo Kan 2 Eo 2006 05110 SueWE Woh KyEND sen 19 sanoy woes 9 were LvCIea/ 9 O61 fa VEOU 0p 4 (01) fesse oc aue o speed eneunad3 pur ou 0a oun eda (oxen) ‘wu serousa: rouey apes: 6 seuev0tey 4 SRS e ‘Wl paNosge sone ensous a He eum © (ores) Lomwomads her sera fapotnuaap st emonas mck aero ore 8 ue> ROH GOHenbe Berg ap aniog omc 1 0 an Ke asi _SSNOHSSY NENG PUY SSREASET AETV UPON, sous oy wes SINLUSNI AoWWayHa NYAVIWNIN i 5 ot BS e 3 Bet g2 : e 2 Ba 2 E a fag si ogg de 2 fe Gai a. Elba i sii ie ai i gas E°C 2 EE vo vay OPUS wean LLALLISNI ADVWAVHa NV)ee ¢ - 3 8 g" = 3 . ee s E 3 fogs § 2 2 o 5 3B 2 8 s = ¢ _ | 7 at yiMALAYAN PHARMACY INSTITUTE Majhitar, East Sikkim-737 136 1% Sessional Examination, November, 2 M-Pharm 1* Semester a! MAT & QA mr ours. Full Marks: 25 1y § questions. eran c a mi , ee Oe Glew, compounds in the following compounds in ethanol Ch 2 Define end absorption in UV spectroscopy. Discuss in details about the . Explain the x-ray diffraction with application. os B+. Types of thermal Analysis? Explain about TG? 05. * Define the term Quality assurance. Write down the standard operating, procedure for it. 1S43.5=5 * What are the requirements and ingredients of self inspection? Discuss in brief about quality audit eyelé. 1.5 +1.5+2 » ¥ ‘Um—CH(OH)—CH OW wy" 3s z § : aa pane Bb? g an go Re, ee 3 B° ae < go ed 3 5 e g * 3: ge = é x HIMALAYAN PHARMACY INSTITUTE ajntar, East Sikkin- 797135 sana Sessional Examination January-2007 pharm 1st Semester on Analy Techniques And uality ASsurane ane? 25 Time: 2 hrs answer any FIVE rom the flown spo apg ution iow cana simple cubic 2 structure be ' araga's x2 spectrometer? (g+7=10) |g whetate cular Vibrations opserved in| Fosse vnnuence ore H-bonding 7 vibration Frequencies. (440=70) overtone Fermi = Resonance fp sampe mandi techniques in (ar6=10) sgpantne ase principle and Experimental procedure of ant-icte 25529 (10) aynatd you mean BY SO certitcation? Explain ne sa¥ert features OF varus quaity system” clements of ISO 9002 (10) ity contol tests for Herb Formulations (10) (10)ob . ‘ eas i 3 iG : i i 7 ei Ac” 196 i a oO i re aes on trea om of Radio- (5) sure ASS mats the aim of GMP? What criteria should be followed in @ (5) ue uit Describe it eee wi 0 8S 5 ; ‘ is 7° 2 /2008 M pnarm/18t gem i ues 1! ne art ae rv Ass ” AND yaL! 501 ull Mare 715 ae tion r nee yestions each SH anevr any ne an questions carry eauel section explain the following terms ip camel ip) seimsPi” splitting ic) coupling Constant (2 NMR. +(e) Explain with inustration the working principle wy bie beam sspectrophorometer- ivity, BeerLambert’s LAW: ib) Define molar absorPt + explain with neat illustration the instrumentation | WAC, P.T.O.oF z Ns 9 L kdoosonseds Bnyemuend pur siseq is Seidiouud oyooson2ads sousuosas seajonu pur soupuosei uids venasi@ au) axedwon (e) “pi io SWOT 10 suone\dde pue seumeay eusunnsu, s HO'HO—(HO)HO—HO. HO~HO—Ho—HO ¢ Adoosoujsads ssew pue YWN hq spunod “09 Sumit} Su eieUUBze;iI9 NOK WM MOH (e) —g bia ‘O1dH #2 vonrivewnnsuy pue adiouud ay squoseg (9) a “suoyeinwio) Ieve4 40 eouesnsse Ayenb ay ssnosig fe “AusesBo;ewosy> puny, jeonusiedng (q) IU UONDEIWID KeEY-X Jo suo ud eu equosag (e) +» -e011dde pue sanbiuyos; ‘sa ‘wonewawnoop Buydweg (9) ‘@snoy JewIUe ud jexYog (q) ‘SPIE JoNUOD Ayjend yo sypny (e) Uo Wunosse ue eM °g 8 dnog 9 INPEUDS Buyuea|o AG NOMENA Bue arnpauRE GuUEDIS ow UIedS OY 8 k snp Bm0. potenes poe ‘eouimmn. 10) pesope weancelan! ant Se NOES, ov z Ns 9 ¢ Kdoasonseds 0) aneiuend pu siseq anne} ‘AN 40 ABojopoyyow enb Ss} yeu 49 ‘Saidiounsd o1doosonoads eoueuese, seajonu Pue eoueuoses uids uonosja ayy oxedwon ( is "SWOT 40 suoHBondde pue soimes) jequewnsjsu) 9 HO'HO(HO)HO—'HO ( HOHO—HO—HO (1) ¢ Adoosongads ssew pue yn Aq spunod “woo Burmorioy ay) oyeQUDIO}}IP MOK IMM MOH (B) “G ty #0 voneuoWnASHY pue ejdj e st Teae4 Jo eouesnsse Ayyenb ay ssnosig (e OUdH ud ayy aquosag (q) “Ayde:Boyewoiyo @ si ~Pou1dde pue senbiuyoay ‘s: JPUE UOHoEIWNIP key. ould ‘onewawnoop Buyjdweg 8NOY JewiUe UG Jomuog (gq) 1 Jonuo9 Aujend yo syipny (e) UO qunoode ue aA 9 8 unolp,) WaqLNSEQ GUN duo Pur PeNOTOL 30 PINOYS BLEND YM Zaid JO UHR at 8 eUNA ) “Aessy oun “APR J0 Suzonpaid aRSOUEEDP 219 LO SEI UI SsMOSIQ Ss ©) ‘Adosonzeds ‘X01 waRshs paneBnuan Jo uogdiosge aya inoqe Weep uy ssrosig \eimeds aysvey, 36D, way ag Ue () ITdH2 uoneardée pue vopewawngsu axa Ayaug ‘ssnosiq »~® ‘UPS? SI Bale pue uD 2/9 5} xyonpucD 49 \A6uaj vaym souepnpuos 2ypads exp sIeIN>}e> “swYO §75 ‘Se 2ueysIsa1 pamoys ‘227208 wnipos yo WORN|OS jeULoUDap y (s) 1 uerdre pue ynau> 2Bpuq auarsieouy oxy meg Usishieue SHRUCRPUCS U NYA 291 UES MOH USD Je0sewNA + ‘Suonsenb any fue sansuy ‘SZ -SMEW JINg Seynun g2 coun Coonror|dde pure Axowonuajog Jo uonetuounnsty 8 VLC Moge wnedxy -y (5) OTdHJ0 uonewaumnsur pure ajdiousg atp aquosaq “9 “PY Apadorg eordesB00p -q ‘PV Auadoug jemaoyjaiuy we | (2x2) Wo sajou YOYs aU °¢ | (=p+1) “Ausnpuy we i warsKs GoHP>31490 0002-1006 OS] wwoHd 01 paurejuteUL 4 Pinoys eua1H9 paw auapmd req ZOsy Aq ueaw nok oP FUN, @) G=z+0) UF POs HoAfos 2p TRoge IAP ut ssnas UW (CW 1 dnos8 jAuogreo J wondsosqe Jo wontsod SR SouaNUY SHOVES aM are THM“ () AU Bt uonesqra zejnsofou axp mnoge jap ut ssnosiq -z (S=sx1) cpoe Suitxoqres pu are}aq9 ‘Yous “jouayg “Toyoope ur yuasaud dno VexorpAt yo wondiosge xp ioays Putog uaBorpKy s30p woK “| (st=sxs) Suoysonb aay Aue somsuy ST 92e yy ny “smog oe coum 8007 *Arenigag“Sel40}810q2} BuNSe} |BOIUIID-UON 10) suoneinBos 719 S91BiS pe3lUN spun suo|s}roud fey ey) Jo yuNoODe Ue NID 4 Buusey reojuio-uon £q weaw nok op YeUN “OL 40S) seinpeso:d Buyesedo prepueys sjuownnsu) uldwes 0} pseBe: u! jou jend jo seniigisuodsa: oy) uo 6 joeds YIM ‘seonoeid Aoye10ge| pooB we, ep Ul ssnosiq § ‘Anewoneds YWN (q) *hewonoeds peisesyu sonbtuyooy Bowmoyor oy Bukuepun ios eu sat Te NOBIOU GUE oYSORSEE "WMO eA eouONe NS! a t : yenbsyo UO JOU B PPy {SenbluYoo jeonfjeue ca spews leaysyeis Addo 0} Kesceoou 3131 Kura(a) ‘dH i. twolssej9 jo sesnyee} jeyuewnsjsu} ey equose (2) -oyewwo.ys: Ayuyye 30° suokiesiidd® pu oftidtr @nBA-yJBY PUB. YOYENdE 91A0¥I| Jo eOUE: \uBig (8) Uo se}ou eI °g @-uonjseg US'S = & ‘"HIQ—eueKaYoIOAD (A) 28% = 8 “'HON'O—ouento, 62 = 8 “tH'9—eueifjeoy 481 = 8 "'H'o—sueztieg (, C spllnbdulos" BuMOH0} ou IS YBolWAYD' ey) uUlBIdx® NOK op MoH (q) “suoneoiidde jeoyneoewieyd wm fesseounuus o1pey uo sejou equyA (2) -¢ "SNOT J suoHBoIddy (9) AudesBoueishio Kes-x sisaioydonoow War mode swap uw ssnasig (oi) “Svonenuue FeGieH JO} isa aquco fyenb sua serang 9) (ou) 2006 O51 0 Suewe[e wayshs Ayenb snouer £9 SSIES, UBIES 24 Ue dhe cuoReayRIED OS} fq URE NOK Op 1eY M, Q (oy) “este 200-18 anpecaid aueuuedeg pur edouu 2809 aM URKdCg ¥ (o1=o+») ‘Wu senbuypa, Buipuey adwegq ‘SOUEUOSEY ~ Ue SUOLEAQ & € (o1=9+9) seruanbary vojesq, uo Bupuoa-H aun yo souenyuy ay ssnosiq 'q Tu Pansesgo suogesai, seInoq|ow aug axe YeyMy e 2 (Ol=z+e) Uatawondeds AeL-y s 662g Ka, pauluejep 9 mnas fish ogo acu veo mo} ‘uonenba 66619 2 eneg ‘| Buwmovoy aun wou Anta Aue sonsuy SAG au stSHeW Ing aa] Li wR JasOUES BL WEY Lope: hrenwer “wore Weuoreses puZ S'1+S'L 9[9ko ypne Anyend moge jaug Pure siuswasmbou om ame wey -9 st 7120) ampaooid mesado pmpuns om unop ayy, aouminse Aujend wa 9 auyeq “5 $0 LOL moge mejdxg csiskreuy jeuuayp jo soddy +p so ‘uoneoridde qu uonsesyip Aes-x amp ured -¢ Sabet ‘wanjossejod jo aouasaud wares | Puno pu aes pouoxs uy dou jfuoqaes jo uoreniguoa aruonsaja | s roge SUED U ssnosiq Adoasonseds 47 m uondhosge pua auyaq -z ho ° ouew9 ut spunoduiog XRWY 3B arena}eD | “suonsonb g fue sausu Sumojioy aq ur spemodwoo Smmonos ax jo st:euey ting “uno4 oe 1 samy= HIMALAYAN PHARMACY INSTITUTE Majhitar, Rangpo, East SikKim-737139 3rd Sessional Examination, February-2006 M.Pharm First Semester 2005-06 Modern Analytical Technique & Quality Assurance Full Marks: 25 : Time: 75 n. (Answer any five questions) ou Explain the basic principle of Bragg’s X-ray spectrophotometer to deem, Crystal structure. ee “explain the principle and Instrumentation of Differential Scanning Cao: 3 wnat do you meati by SQC. How you use SQC processes for product «ni a quality control Laboratory. Given an example of quality control chart» determine the UCL and LCL. <4 Write in brief about the mechanism of measurement of a drug by IR ‘Spectroscopy. What do you mean by finger print région? Write briefly the effect of ii™ boyding for alcohol, phenol, enol, chelates, carboxylic acid, amine in |® . pectroscopy. : 6/ _ Discuss in detail about the instrumentation of a Double focus Spectrophotometer. sing Mass a —BS GONE OS! PHT MOL 1S rOROWE OU USP s — © S80082%8 JO wORDES LO UNESP UP BUM (@) Apeewne Bowie, ee equssag (#) = s “Sseroydauags Sun sus 3 “souenbeyj jeuonzian uo Guipueg vaboupAy 30 sowengur ay SSMS ¢ SouBuesas iwey Bee Szuusno 8 Yew {uondiosge ut uo Panonut SORE: TeMoGOW MREUD Oy are rey ® ¥ drop Hew I creep uiBrew eur wy Sainby ayy Grei® YORE wor} suonsond sem: Aue sonsuy St—oyeN img, Sunoy sney—suny \oe—receg ZANYENESY LUNWNS ony SANOINAL woe HEZCoR, woe, oA ova is Lu, Sapo,joej@ JO uoyaa!@s uo juNosoe vor cuondiosqe YI! uo paAjonul IK JeIMOGIOW WwaLeyIP oy) ee YUM (2) “| ¥ dno “Syrew fIN} e20IpU) WiBieW ey 4 yy ‘dn0i8 yoee wos suonsenb seiy: Aue somsuy Steen jing Sinoy anoy—uiiy t0g—iodeg oNvunssy ALnvno any & yey HIMALAYAN PHARMACY INSTITUTE Majhitar, Rangpo, East Sikkim-737132 4* Sessional Examination, November-2005 M.Pharm First Semester 2005-06 Modern Analytical Technique & Quality Assurance. Full Marks: 25 Time: 75 minus Answer any five questions oN a a) What are the effects of polar solvents af nonbonding electron in carbonyl grou? b) _Define charge transfer spectra & End absorption c ° Explain hyper chromic & hypo chromic effect . cont 2) Calculate the Anus for an absorption band in the UV spectrum of he folowing = ok “ ma b) For a- unsaturated ketone what numerical corrections should be than ethanol is used? os-Pnt Give an account of the PAGE of protein. What are the applications of s Pd tore in cond é ‘yf The strength of titrant js ten times stronger than the titrate Justify b)/ What do you mean by dead stop end point? Why is it called 80? : ‘What is the composition of Karl-Fischer reagent? Explain its function me \ What do you mean by Good Manufacturing practices (GMP)? types of mandatory documents are necessary in a GMP section? Describe the steps used to prepare a Master Formula Reco Procedurei, 4 M. Pharm/tstsy, 2008 MODERN ANALYTICAL TECHNIQUES AND QUALITY ASSURANCE Paper—s01 Time—Four hours Full Mets Answer any three questions from each Sects: All questions carry equal marks. Section—A 1. Explain the following terms : (a) Chemical shift (©) Spin-spin splitting (6) Coupting Constant » (d) C# NMR. ing 2. (a) Explain with illustration the Wo °f double beam spectrophotometer. amber? al et? (©) Define molar absorptivity, Bee! ios! 3. Explain with neat illustration the of HPLC,Full Marks: 25 HIMALAYAN PHARMACY INSTITUTE Majhitar, Rangpo, East Sikkim-737132 2" Sessional Examination, February-2006 M.Pharm First Semester 2005-06 Modern Analytical Technique & Quality Assurance. f@) (b) (a) (b) Time: 75 m= ‘Answer any three questions horesi Explain the procedure of moving Boundary Electrop! a8 Describe its uses, What is the continuous diagram and its uses. Electrophoresis? Define RIA, determine its With the competitive binding characte curve of Radio-immune2ss2Y. plications? and its applic: ue What is the principle of HPLC and ifs 3 matography Discuss the principle of Gas Chro vali 7 qualitative analytics. oot wep e What is the principle of antibiotic Procedure of antibiotic assay. yin ro Discuss the ion exchange chromatog”? y e”