similar in appearance, even when imaged using high- resoiution electron microscopy. — small in size — very little cytoplasm is observable — rounded cells in vivo as well as in cell culture — Under normal cell culture conditions, lymphocytes do not adhere to the substrate. Macrophages. can adhere to the substrate during growth in culture — not as tightly as epithelial and fibroblasts — results in a more spherical shaped cell — can be identified by the presence of vimentin. Material/Equipment. 1, Fibroblast cell 2. Epithelial cell 3. Lymphocyte cell 4. Macrophages 5. Fixing agent 6. Metethylene blue 7. Slides 8. Water 9. Pipette 10. Tissue Procedure. 1. All 3 slides with cells add 1, 2 drops of fixing agent 2. Incubate | min 3. Rinse with water 4. After add metethylene blue 5. Incubate 2 min 6. Rinse with water 7. Add cosin 1, 2 drops without incubate immediately rinse with water 8. Observe in microscopeIssue Procedure. a 1. All 3 slides with cells add 1, 2 drops of fixing agent 2. Incubate 1 min 3. Rinse with water 4. After add metethylene blue 5. Incubate 2 min 6. Rinse with water 7. Add eosin 1, 2 drops without incubate immediately rinse with water 8. Observe in microscope Result. Fig 4. Fibroblast 1 sample were lymphocytes because — small in size — very little cytoplasm is observable 2- epithelial have irregular shapes 3 macrophages — not as tightly as epithelial and fibroblasts — results in a more spherical shaped cell 4- fibroblast have fusiform: wide in the middle and tapered at the ends.Introduction. Cells that are derived or isolated from tissue of a mammal * major types: — Fibroblast — Epithelial cells —Lymphoblast Fibroblast. In vivo, fibroblasts exist as single fusiform cells embedded within connective tissue. — Fusiform: wide in the middle and tapered at the ends. Play an important role in normal physiological processes such as wound healing by secreting matrix proteins, growth factors and cytokines. Epithelial cells. + three possible shapes in the body — squamous — cuboidal — columnar They are found throughout the body, including the epidermis, digestive system, reproductive system, and endocrine system. Cell Culture: — adhere tightly to the substrate, resulting in flattened cell morphology — form a contiguous monolayer due to contact inhibition — The intermediate filament protein keratin is used as a cellular marker Limphocytes. Unstimulated T and B cells are very similar in appearance, even when imaged using high- resolution electron microscopy. - small in size — very little cytoplasm is observable — rounded cells in vivo as well as in cell culture — Under normal cell culture conditions, lymphocytes do not adhere to the substrate. Macrophages. can adhere to the substrate during growth in culture —not as tighbheassepitbelialand fibroblasts