Technical Data 63

2.8.4.13 Photometer calibration

The photometer calibration procedure is completely controlled by the software.
It must be performed if the lamp has been replaced or the energy of the original
lamp became too low.

If the gain-stage of measurement or reference channel is higher than 10 it is

recommended to replace the halogen lamp. During Calibration the light path
must be free but protected against ambient light. A cuvette or Flow-cell must
be removed.

Filter: The wavelength of the interference filter

m-gain-stage: The measurement branch amplifier gain (0…12), factor 2 be-
tween the gains
m-gain-stage: The reference branch amplifier gain (0…12), factor 2 be-
tween the gains
m-bright: The measurement branch AD-converter digits for the bright
value
r-bright: The reference branch AD-converter digits for the bright val-
ue
m-dark: The measurement branch AD-converter digits for the dark
value
r-dark: The reference branch AD-converter digits for the dark value
r-bright-ref: Copy of the reference branch AD-converter digits for the
bright value used for monitoring of the lamp energy loss

Figure 62
 64

• Press Selftest then,

• Press Calib Photom and the window with current photometer calibration
values appears:

Figure 63

• Press OK to start a new calibration and the following message appears:

Figure 64

• Remove the Cuvette or Flow Cell.

• Press Yes.

Figure 65

¾ Note, that this will take several minutes...

¾ If the calibration is finished a screen with the New Photometer calibration
values appears:

Figure 66

HumaLyzer 4000 | Service manual

Technical Data 65

• Press the according button to start the calibration.

¾ Select REPLACED if the lamp has been replaced. The factory reference bright
values are overwritten.
¾ Select UPDATE if the original lamp has to be recalibrated. The factory refer-
ence bright values remain valid.
¾ After the (successful) calibration the following window appears:

Figure 67

• Press OK to continue.
¾ After the calibration it is possible to reset the values to the previous ones.
¾ An additional button appears to undo the calibration.

Figure 68
Photometer Calibration

¾ Only in this processing step the button appears and Undo Calib is possible.
¾ If OK has been pressed and the user returns to the screen the Undo Calib
button has disappeared.
• Press Undo Calib to open the following window.

Figure 69

• Press Yes to automatically reset the calibration.