Substrate Concentration

https://chem.libretexts.org/Courses/Saint_Francis_University/
Chem_114%3A_Human_Chemistry_II_(Muino)/19%3A_Enzymes_and_Vitamins/
19.05%3A_Effect_of_Concentration_on_Enzyme_Activity#:~:text=Initially%2C%20an
%20increase%20in%20substrate,the%20concentration%20of%20an%20enzyme.

https://www.youtube.com/watch?v=SzYFc4rlo_4

For the Lab :

https://www.region18.org/uploaded/Student_Stand-Outs/Jessica_Lee_Enzyme_Lab.pdf
In the enzyme (catalase) concentration lab collect eight beakers to prepare with eight different
concentrations of catalase (40 mL, 32mL, 30 mL, 24 mL, 20 mL, 10 mL, 4 mL, and 0mL).Add distilled
water to reach a total solution of 40mL. A filter paper disc is then immersed into the solution of catalase
and then placed at the bottom of each substrate solution of 1.0% hydrogen peroxide. Time how long it
takes for the disc to rise to the surface for each because this is the rate of the reaction (it rises because
of the oxygen produced from the reaction).

For the substrate concentration, prepare nine beakers of varying hydrogen peroxide concentrations (0.0
mL, 1.3 mL, 2.7 mL, 4.0 mL, 6.7 mL, 10.7mL, 13.3 mL, 26.7 mL, 40.0 mL). Carry out the same procedure
as the enzyme concentration by putting the filter paper into a solution of 100 units/mL of enzyme then
placing in the different solutions of hydrogen peroxide and measure the time it took for the filter paper
to rise to the surface.

So we’ll use 5 concentrations instead of 9 (0,10,20,30 and 40 mL)