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  • Alur Dna

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    KU21012Mahrunisa Nur Afifah
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    1. Epithelial cells were collected from gargling in isotonic solution and placed in a beaker. 2. The epithelial cell solution was centrifuged multiple times to separate residues from filtrates. Tris-EDTA buffer was added to the final residue and mixed. 3. NaCl and ethanol were added to the cell solution to precipitate out DNA, forming DNA clumps.

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    1. Epithelial cells were collected from gargling in isotonic solution and placed in a beaker. 2. The epithelial cell solution was centrifuged multiple times to separate residues from filtrates. Tris-EDTA buffer was added to the final residue and mixed. 3. NaCl and ethanol were added to the cell solution to precipitate out DNA, forming DNA clumps.

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    6 views2 pages

    Alur Dna

    Uploaded by

    KU21012Mahrunisa Nur Afifah
    1. Epithelial cells were collected from gargling in isotonic solution and placed in a beaker. 2. The epithelial cell solution was centrifuged multiple times to separate residues from filtrates. Tris-EDTA buffer was added to the final residue and mixed. 3. NaCl and ethanol were added to the cell solution to precipitate out DNA, forming DNA clumps.

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    of 2

    1.

    Collection Cell

    10 mL isotonic

    - Gargle for 1 minute


    - Accommodated in A Beaker

    Epithelial cells

    2. Cell Splitting (Lysis Cell)


    Isotonic epithelial
    cells

    - Take as much as 1 mL of epithelial cell


    solution using a volume pipette
    - Inserted into the microtube of the
    centrifuge
    - Centrifuged at 10,000 rpm for 1 minute
    - Decantation

    Residue Filtrate

    - Add1 mL of epithelial cell solution,


    repeated the previous procedure up to 2
    repetitions

    Residue Filtrate

    - Added 1 mL of Tris-EDTA buffer


    solution into the microcentrifuge tube
    - Divortex for 30 seconds
    Result

    3. DNA Precipitation
    100µL of NaCl 2.5 M
    - Inserted into a microcentrifuge Tube containing the sample
    solution
    - Stir the contents of the tube so that it is mixed well, by going
    back and forth with the tube
    - Transferred all contents of the Test Tube to another clean
    and dry tube
    - 1 mL of ethanol is added slowly (to precipitate or coagulate
    DNA)
    - Leave it on for 5 minutes to produce clear boundaries
    DNA clumps

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