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    © All Rights Reserved
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    7 views12 pages

    Choi & Jang (2009a)

    Uploaded by

    Kharisma N. Puspitasari

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    of 12
    Journal of Soonchunhvang Medical Science 14(3) p.78i~794 Feburary 2008 7 Bactericidal Effect of Medilox, an super oxidized water generated by Medilox against Bacteria, Fungi and Mycobacteria Tas Youn Choi, Eui Young Jang Department of Laboratory Medicine, Soonchunhyang University Hospital, Seoul, Korea = Abstract = Background : Disinfection is essential for the prevention of hospital infection. Medilox, an super oxidized water generated by Medilox (SOOSAN E&C CO.,LTD. Yongin, Korea) was developed as a disinfectant in Korea. This is not costly and does not cause any clinical problems and environmental pollution, We evaluated bactericidal activity of Medilox against several clinical isolates and standacd strains. Method : Clinical isolates and reference ATCC strains were exposed to Medilox, an super oxidized water (80 ppm of HOC!) generated by Medilox (SOO SAN E&CCO., LTD. Yongin, Korea) for the various periods (0.5, 1, 2, 5, and 10 minutes), After the exposure mixture of microorganisms and Medilox solution was inoculated into tryptic soy broth and onto tryptic soy agar, Sabouraud dextrose ayar or Ogawa medium and cultured at 35°C, Results : All strains of bacteria, yeasts, mycobacteria and vegetative forn of Bacillus subtilis were killed within 30 seconds after an exposure to Medilox (80 ppm of HOCI) under elean and dirty conditions. But, spore form of Bacillus subtilis was killed within 5 minutes, Conclusion: Irmay be recommended that Medilox can be used for the effective disinfectant for hospital cnvironments and high-level disinfectant for hospital infection control. Key Words : Disinfectant, Medilox, Hospital infection control, super oxidized water Introduction There are lots of methods used to prevent an. infection by pathogenic ot opportunistic ‘Author: Tae Yoon, Chat infection in the hospital, including washing, Adress: Laboraary Medicine dep, Soonchenhyan disinfection, and sterilization." Disinfectants vert spit 87, Hannon, Yong, Sel, > Koren 120-745, rs Yoresares, S04 are defined as the agents that fully kill the “Tah 2-709.9435, ! Fax 02-790-5820 fungi and vegetative bacteria causing an infection Emil: choity@bosp.sch ac kr srk ‘gustan ote ‘within 10 minutes, not anes that exterminate the Publish Confirmed Date: Feb 3, 2009 (M) >) (¥) clostridial spores. 2. Journal of Soonchunhyang Medical Science Vol.14 No.3 Feburary 2009 Disinfectants are divided into a low level that can Kill bacteria, virus oF some finngi but not the myco~ bacteria or clostridial spores; a mid level thar actually kills the mycobseteria or fungi but not the clostridial spores; anid a high level one that with enough time of exposition can kill even the clostridial spores. Disinfection involves different medicines and methods depending on the subject to be disinfected ; and nevertheless, selecting of proper disinfectants is important in the economic aspect and eritical in terms of the effect of disinfection. Of the disinfectants used in the hospital, chlorine cormpounds ate can apply to a wide range of treatment, work fust and cost lower than other compounds bur the hypochlorous acid (HOCH) indicates: big yapin antiseptic effects with their different pli densities." With the brisk activities of infection management undertaken by hospitals, the super oxidized water containing the HOCI with a great antiseptic effect with water and clorine components electrolyzed is used as a disinfectant; its range of use is also expanding," LOCI is a disinfecting water thet is generated at the point when the electrolyte containing is electr~ olyzed around the neutral part, The mechanism in which this disinfectant works are not known explicitly but it seems fo work in the way an activated oxygen is oxidized inside a cell to mitigate the enzyme Functions while the HOCI creates a combination of nitrogen and chlorine, denatures the protein and inactivates the nucleic acid at the cell membrane and in a cell to introduce a electric shock to microorganisms with higher pH and transposition of oxidution-restoration: A-common low-tcid HOCLis colorless, odorless, and non-stimulus so poses no harm to human bodies and used in its Hiquefied state, In addition, it works so fast that itis used in a wide range of areas fiom washing of fruits and vegetables, disinfection of instruments under the Food Safety Law to disinfection of non- hazardous instrumenis used in hospitals and pseudo- hazardous ones such as an endoscope"? Since the ucteriokiller system (TRP, Japan) was first developed in Japan as a running water type disinfecting device ‘gencrating HOCT, the Medilox (S90 SAN E&C CO., LTD, Korea) and WATRIX-10G(MieroBank, Korea) were also developed in Kozea as well as Stetilox (St- crilox medical UK Ltd., Abingdon, Oxon, UK) and the Cleantop (Ka-igen Co., Japan), sn endoscope dedicated disinfecting device utilizing the LOCL The authors evaluated the disinfecting capacity of the HOCT, Medilox, generated by « HOCI generating device, Medilox (S00 SAN E&C CO.,LTD. Yongin, Korea) developed in the nation with pathogenic and standard strains collected from the patients in Soon- chunhyang University Hospital, based on the time they are exposed to the disinfecting agent, Subjects and Methods of the Study 1. The Subject Bacteria Types and Strains A total of 6 strains of gram-positive bacteria sepa- rated from the specimens of inpatients at Soonchun- hyang University Hospital including methicillin- aureus (1 strain), susceptible Staphylococens Journal af Soonchunhyang Medical Science 14(3) p.785~794 Feburary 2009 3 methicillin - resistant Staphylococcus aureus (1 strain), methicillin - susceptible Staphylococous epidermidis (1 strain), methicillin-resistant Stap- hylococeus epidermidis (1 strain), vacomycin- resistant Enterococcus faccium (1 strain), and Enterococcus facealis (1 strain); a total of 12 strains of Gram Negative Bacillus including Sal- monella Typhi (1 strain), Salmonella Eateritidis (1 strain), Shigella sonnei (1 strain), Pseudomo- ‘nas aeruginosa (1 sirain), Acinetobacter bauma~ nnii (strain), Escherichia coli (1 strain), Serratia marcescens (1 strain), Klebsiella pneumoniae (1 strain), Enterobacter cloacae (1 strain), Citrobac~ ter freundii (1 strain), Stenotrophomonas malto- philia (1 strain), and Proteus mirabilis (1 strain); a total of 2 strains of fungi including Candida albicans (1 strain) and Candida glabrata (1 strain); a total 5 strains of mycobacteria including Mye- obacterium tuberculosis (5 strains), Mycobacter- jum avium (1 strain) and Mycobacterium absce- ssus (1 strain), The standard strains of bacteria include Staphylococeus aureus ATCC 2921 coli ATCC 25922, Enterococcus faecalis ATC 29212, Pseudomonas acruginosa ATCC 27853, and Bacillus subtilis ATCC 6633. 2, Subject Disinfectants Employed as disinfectant into the study is Medilox (pH 5.7, HOCL 80. ppm) generated by Medilox (SOO SAN F&C CO., LED. Yougin, Korea), a HOC! generating device. 3. Evaluation of Disinfecting Capacity Candida albicans was cultured in the tryptic soy broth (TSB) for 48 hours; others for 18 hours. The mycobacteria were cultured in Ogawa culturing room for 6 weeks, The bacteria were disinfected at 3,000 rpm for 15 minutes with the upper layers discarded; 10 mL of phosphate buffered soluiton(pH 7.2) put into the precipitated bacteria and wafted evenly with a mixer so that the turbidity be fixed to McFarland No. 3. Each of 0.5 ml bacteria solutions were put in 4.5 mI. physiological saline solution and diluted in TSB. containing 0.75% of lecithin from 10 to 10" times step by step to produce fre final 107~L0* CFU/mL. of bacteria, Each 0.5 mL bacteria solution were added to Medilox 4,5 ml. to be kept ina room temperature; the exposition time was set to 0.5. 1, 2,5, and 10 minutes; with the bacteria and fungi were inoculated with the disinfectant of 0.1 mL. in SmL TSB broth containing lecithin 0.75%; the mycobacteria were cultured after inoculated to MGI culturing solution (MGIT system, Be- cton Dickinson, USA) for the observation of whether the bacteria develop. Besides, to calenlate the viable coll numbers and estimate the develo- pment of bacteria, a phesed dilution was applied to the solution; 0.01 mL of standard platinum loop was used to culture the bacteria in tryptic soy agar (TSA), Candida species in Sabourand, dextrose agar, and Mycobacter- ium tuberculosis in Ogawa culture ground, To identify the antiseptic effect when the or ganic matters exist, the bacteria solution was added to the standard bacteria strain until the serum density reaches up to 3%; the test was repeated in the same way. 4 Journal of Soonchunhyang Medical Science Vol.14 No.3 Feburary 2008 Results 1, Bactericidal effect agains( Gram - Positive Bacteria ‘The number of all bacteria strains including Methicillin - susceptible Staphylococcus aureus, methicillin - resistant Staphylococets aureus, methicillin - susceptible Staphylocacens epider ~ midis, methicillin - resistant Staphylococcus epi - dermidis, vacomycin - resistant enteroeucens far ecium, Enterococcus feaculis, Staphylococens aureus ATCC 29213, and Enteracocens faecalis 29212 decreased more than 5 log10 per 30 second of exposition time at 80 ppm of HOCT (the rem- aining bacteria less than 1x 10 /mL) (Table }). 2, Bactericidal effect against Gram - Negative Bacteria The number of all bacteria swains including Salmonedta Typhi, Salmonella Enteritidis, Shige~ Hla sonnei, Pseudomonas: acraginosa, Acinetoba- ctor baummunii, B. coli, Serratia marcescens, Klebsiella pneumoniae, Entero - bacter cloacae, Citrobacter freundii, Stenotro ~phornonas malto- philia, Proteus mirabilis, E.coli ATCC 25922, and Pseudomonas aeruginosa. ATCC 27853 dro~ pped more than $ log}0 per 30 second of exposi- tion time at 80 ppm of HOCT (the remaining bac- teria less than 1x 10/mL) (Table 1}. 3, Bactericidal effect against Yeast Fungi The number of one strain of Candida albicans and Candida glabrata, all declined more than 5 Jogi0 per 30 second of exposition time at 80 ppm of HOCI (tie remaining bacteria less than Lx 10° fmL) (Table 1). 4, Bactericidal effect against mycobacteria (lable 1). Journal of Seonchunhvang Medical Science 143) p. 785-704 Feburary 2009 $ Table 1. Biocidal activity of Medilox (80ppm of HOCI) against various nicroorganisms according to exposure time Initial Colonies remaining (CFU/mL) ‘Test microorganism count (CFUimL) OS min Linin 2mn Smin 10min Methicillin-susceptible S.aureus 82x10) <10° <10° <10" <10° <10° Methiciltin-resistant S. aureus sgxia’ <10" <10° <10° <10° <10° Methicillin-susceplible S. epidermidis 7.0x10° <10" <10" <10° <10" <10" Methicillin-resistant S. epidermidis 8.9x10" <10° <10° = 1 <10° << 10° Vancomycin-resistant E. faecium T7xlo <10° <10° <10' <10° <10° Enterococcus faecalis 91x10) <10 <10° < 10 <= 10° Escherichia coli 88x10 <10° <10°

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