E.R. tubules (Desmotubules) help to maintain continuity of cytoplasm.
The cell wall and middle lamella may be transverse by plasmodesmata which connect the cytoplasm of neighboring cells. Cell wall materials (hemicellulose, pectin) are synthesized in plant Golgi bodies or dictyosomes. Formation of cellulose occurs by cell membrane. Primary cell wall gradually diminishes as cell matures. It is composed of cellulose, hemicellulose, pectin and proteins. It is capable of growth. Secondary cell wall is absent in meristem cells. Oligosaccharides of the glycolipids and glycoproteins on the outer surface of plasma membrane are involved in the cell-to-cell recognition mechanism. Best examples of cell recognition are fertilization, (where sperm and egg recognize each other) and blood-antigens. Cholesterol is also present in plasma membrane. Cholesterol are more rigid than phospholipids. So, it helps in stability of membrane structure. Cholesterol is absent in membrane of prokaryotes. Thus, Hopanoids (pentacyclic sterol) provides stability to prokaryotic cell membrane. Phospholipid layer provides fluidity to plasma membrane because phospholipids are rich in unsaturated fatty acids. Term cytoplasm was given by Strasbruger for the part of cell, present between the nucleus and cell membrane. Cytoplasm can be divided into two parts: cytosol and trophoplasm. Cytosol- Liquid matrix of cytoplasm Trophoplasm- Part of cytoplasm containing organelles and non-living inclusions (deutoplasm). Microsomes are pieces of ER with associated Ribosome particles. These can be obtained by fragmentation and high-speed centrifugation of cell. They do not exist as such in living cell. scientist used microsome for in vitro protein synthesis study. Most of the macromolecules which are to be sent outside the cell, move through the Golgi body. So, Golgi body is termed as “Director of macromolecule traffic in cell” or middle men of the cell. Space between cell wall and cell membrane (periplasmic space) in bacteria, may play role of lysosome. In plant cells, large vacuole may function as lysosome by storing hydrolytic enzymes so in higher plants lysosomes are less frequent. But number of lysosomes is high in fungi. With exception of mammalian RBCs, they were reported in all animal cells. Cathepsin enzyme of lysosome digests the tail of tadpole larva of frog during metamorphosis. Water soluble pigments anthocyanin is found in cell sap. Number of mitochondria depends only upon the physiological activity of cell. One in Microasterias, Chlorella fiscal (algae). Usually, plant cells have fewer mitochondria as compared to animal cell. In higher animals’ maximum mitochondria are found in flight muscles of birds. Other names of mitochondria- 1. Cell within cell 2. Most busy and active organelle in the cell 3. Semi-autonomous cell organelles 4. Endo-symbionts of cell Elementary particles were first described by Fernandez Moran. Mitochondrial DNA is 1% of the total DNA in a cell. It is rich in G-C content. Chromoplasts occurs mainly in pericarp and petals. Red colour of tomatoes is due to the red pigment lycopene of chromoplasts. Stroma lamella or Frets channel or stroma thylakoids are flat membranous tubules connecting the thylakoids of the different granum. Karyotype: Karyotype is external morphology of all chromosomes of a cell which is specific for each species of living organisms. Karyotype includes the number of chromosomes, relative size, position of centrosome, length of the arms, secondary constrictions and banding patterns. Idiogram: Diagrammatic representation of karyotype. In idiogram chromosomes are arranged in decreasing order of size. Sex chromosomes are placed in last. Idiogram is specific for every species. Axis of lampbrush chromosomes is consists of DNA, while matrix consist of RNA and proteins. Lampbrush chromosomes is concerned with Vitellogenesis (yolk formation). Salivary gland chromosomes concern with metamorphosis and moulting. At centromere, two protein discs are present which are called kinetochores. Kinetochores constitute the actual site of attachment of spindles to chromosomes during cell division. Secondary constriction is also known as NOR (Nucleolar organizer region). In humans NOR is found in chromosome number 13,14,15,21,22. Part of chromosome remains after NOR is known as satellite. Chromosomes have polarity and polar ends pf chromosomes are known as telomeres. Telomere prevents fusion or sticking of one chromosome to another chromosome. Telomeres are rich in Guanine bases (5’-TTAGGG-3’). Enzyme telomerase which is a ribonucleoprotien synthesis telomere Parr of chromosome. Telomere of chromosome become shorter during ageing process. Larger and more numerous nucleoli are present in cells actively carrying out protein synthesis. Chemically, chromatin consists of DNA, RNA, histone proteins (basic proteins, rich in Arginine and lysine) and non-histone proteins. Generally eukaryotic cell contain atheist one nucleus is absent in mature sieve tube cells of vascular plants and mature erythrocytes of many mammals. Nucleus is considered as controller or director of cell. Importance of nucleus in control of hereditary, growth and metabolism were experimentally proved by Hammerling. (Experiment was on Acetabularia a single cell largest alga) Larger subunits (50S) of 70S ribosome contains peptidyl transferase enzyme (23S rRNA) which helps in the formation of peptide bond during protein synthesis. This is a sample of ribozyme. EXTRA QUESTIONS The ribosomes of chloroplasts are smaller than cytoplasmic ribosomes. Proteins present in plasma membrane are classified as integral and peripheral on the basis of ease of extraction from membrane. Carbohydrates that are present in cell membrane take part in cell recognition. Plasma membrane is a fluid structure due to presence of i. Carbohydrates ii. Lipids iii. Glycoproteins iv. Polysaccharides Which of the following is capable of growth and gradually diminishes as the cell matures? i. Primary cell wall ii. Secondary cell wall iii. Muddle lamella iv. Cell membrane The fluid nature of the cell is helpful in function of i. Cell growth ii. Cell division iii. Endocytosis iv. All of the above A single unit membrane organelle is i. Ribosome ii. Mitochondria iii. Chloroplast iv. Lysosome
Detoxification of lipid soluble drugs and other harmful compounds in
endoplasmic reticulum is carried out by cytochrome P450. Anthocyanin pigments is found in cell sap. Salivary gland chromosome is concerned with i. Vitellogenesis ii. Formation of ribosomes iii. Lipid synthesis iv. Metamorphosis in some insects The telomeres of eukaryotic chromosomes consist of short sequence of guanine rich repeats. Nucleus is absent in i. Cell of vascular cambium ii. Root hair cell iii. Companion cell iv. Members of mature sieve tube
Self-duplication doesn’t occur in
i. Mitochondria ii. Centrioles iii. Chloroplast iv. Ribosome The physiochemical approach to the study and understand living organisms is called i. Physiochemical biology ii. Reductionist biology iii. Fundamental biology iv. Biochemical biology Which type of solutes may move across plasma membrane from higher to lower concentration along concentration gradient without the help of transmembrane proteins? i. Positively charged solutes ii. Negatively charged solutes iii. Neutral solutes iv. Any of the above If living cell, similar to those found on earth, were found on another planet, where there was no oxygen, then which cell organelles would most probably be absent? i. Ribosomes ii. Golgi apparatus iii. Mitochondria iv. ER Ratio of protein and lipid in the membrane of erythrocytes of human being is 1.3. A student done the sell fractionation of a tissue and first to laver his tubes. The content of one tube when studied showed organelles bounded by membrane with activity of catalase enzyme. These organelles could be peroxisomes. How many types of cells are known? Three The division of the plant kingdom into Prokaryota and Eukaryota is based on the characters of i. Nucleus only ii. Chromosomes only iii. Cell organelles only iv. All of the above T. Schwann and M. Schleiden were German scientists. Which of the following is seen only in prokaryotic cells? i. Dictyosome ii. Ribosome iii. Mesosome iv. ER v. DNA Cytosomes are found in i. Chloroplast ii. Bacteria iii. Mitochondria iv. All of these Schleiden proposed that cell is the structural and functional unit of life. His idea was a hypothesis. The size of most of the cells is the best expressed in micrometers (um). Robert Hooke used the term cell in the year 1665. The strength and rigidity of the cell wall is due to the substance are known as lignin. The most abundant substance of middle lamella is Pectin. Cell wall is absent in i. Gametes ii. Amoeba iii. Mycoplasma iv. All of these What is the structural element of cell wall? i. Matrix ii. Microtubules iii. Microfibrils iv. Arabinogalactans The chemical substances found most abundantly in the middle lamella is released into the phragmoplast by Golgi complex. What is common in the structure of cell wall of bacteria and fungi? Both are made up of N-acetylglucosamine For cell membrane, name plasma lemma was given by Plowe. Beetroot if kept in cold water anthocyanin doesn’t come out due to the plasma membrane impermeable to anthocyanins Keeping in view the fluid mosaic model for the structure of cell membrane, which of the following statements is correct with respect to the movement of lipids and proteins from one lipid monolayer to another (described as flip flop movement) i. While proteins can flip flop, lipids cannot ii. Neither lipids, nor proteins can flip flop iii. Both lipids and proteins can flip flop iv. While lipids can rarely flip flop, proteins cannot Lipid molecules in plasma membrane are arranged in head parallel. On which surface of cell Donnan equilibrium occur? Plasma membrane According to the ‘Unit membrane model’ the thickness of the cell membrane is about 7.5 mm. Which of the following constituents of biological membrane? i. Phosphoprotein ii. Proteins and phospholipid iii. Phospholipids iv. Cellulose Single membrane bound organelles are i. Lysosome ii. Sphaerosome iii. Glyoxysome iv. All of these The cell membranes of adjacent cells are fused at this cell junction Zonula occludens. Lomasomes are found in fungal cell. Singer and Nicholson’s model of plasma membrane differs from Robertson’s model in the arrangement of proteins. The name protoplast was given by Purkinji. The amount of which element is greatest in protoplast? Oxygen Normal pH of protoplast is 6.8. Protoplasm is a i. True solution ii. Suspension iii. Emulsion iv. Polyphasic colloidal solutions Protein synthesis in an animal cell, takes place in the cytoplasm as well as in mitochondria. Protoplasm was regarded as the physical basis of life by Huxley (1868). Mitochondria perform all of the following functions except i. Nucleic acid synthesis ii. B-oxidation of fatty acids iii. ATP synthesis iv. Polysaccharides degradation In mitochondria, protons accumulate in the intermembrane space. Prokaryotic origin of mitochondria was proposed by Altmann and Schimper. The number of mitochondria increases in cells of i. Dormant seeds ii. Germinating seeds iii. Dry seeds iv. Dead seeds In prokaryotes, the mitochondria are absent. Even then Kreb’s cycle takes place. What us the site of Kreb’s cycle in bacteria? Mesosomes What is mitoplast? Mitochondria without outer membrane Racker’s particles are found in ……… Or Fernandez Morgan particles are seen in mitochondria. Who first introduced the term mitochondria? Benda Which of the following cell organelles is considered to be rich in catabolic enzymes? Or Respiratory and oxidative enzymes are present in mitochondria. In mitochondria, enzymes cytochrome oxidase is present in inner membrane. The cristae of mitochondria possess oxysomes. Mitochondria is non-existent in some bacteria. Mitochondria supply most of the necessary biological energy by i. Breaking down of sugar ii. Oxidizing substrates of TCA cycle iii. Reducing NADP iv. Breaking down of protein Centre of phosphorylation is oxysome. In which part of mitochondria, ATP is generated? Oxysomes (F1 particles) In higher plants, the shape of the chloroplast is discord (lens). Which of the following is incorrect? i. The inner membrane is highly convoluted forming a series a infoldings ii. The outer membrane resembles as sieve iii. The outer membrane is permeable to all kinds of molecules iv. The enzymes of the electron transfer chain are embedded in the outer membrane The term chromatography was coined by Schmitz. Quantasomes are found in chloroplast. The main difference between chlorophyll a and B is in chlorophyll a there is CH3 group whereas in b it is -CHO group. In land plants the guard cells differ from other epidermal cells in having i. Chloroplast ii. Cytoskeleton iii. Mitochondria iv. ER Match column-1 with column-2 and select the correct option. Types of chloroplasts Algae A. Cup shaped 1. Ulothrix
B. Girdle shaped 2. Oedogonium
C. Stellate 3. Chlamydomonas
D. Reticulate 4. Zygnema
A-2, B-4, C-3, D-1
Water soluble yellowish pigment present in the petals of Dahlia is anthoxanthin. The amyplasts look like proplastid. When green tomatoes fruits turn to red, then i. Chloroplasts are disintegrated and get converted into chromoplasts ii. New chromoplasts are formed iii. Chromoplasts are changed to chloroplast iv. None of the above All plastids have essentially same structure because i. They have to perform same function ii. They are localized in aerial parts of plant iii. All plastids store starch, lipids, and proteins iv. One type of plastids can be differentiated into another type of plastid depending on cell requirements Many cells function properly and divide mitotically even though they don’t have i. Plasma membrane ii. Cytoskeleton iii. Mitochondria iv. Plastids Which type of membrane is most abundant within a cell? i. ER membrane ii. Nuclear membrane iii. Golgi membrane iv. Plasma membrane ER is more developed in i. Green cells ii. Young cells iii. Mature cells iv. Bacteriophage The transfer vesicle from RER fuse with which region of Golgi complex? Cis Nuclear membrane is formed around the groups of daughter chromosomes during the telophase by endoplasmic reticulum. In rapidly developing cells, endoplasmic reticulum is poorly developed. Main function of dictyosome is secretion. The cisternae that make up the Golgi complex are i. Rough ii. Polarized iii. Non polarized iv. Reticulate Dictyosome serves as a temporary storage place for proteins and other compounds synthesized by ER. ER is in continuation with nuclear wall. In plant cells, number of Golgi bodies increases during cell division. Secretary and membrane proteins are processed in Golgi complex. The element responsible for the ring structure of chlorophyll and maintenance of ribosome structure is Mg+2. Peptide synthesis takes place inside a cell in ribosome. In active leaf cells, double membrane is absent in lysosome. Who discovered ribosome in animal cells? Palade Ribosome similar to those of bacteria are found in pancreatic mitochondria. Which of the following statements is wrong for ribosome? i. Formed by two-sub units ii. Formed by riboprotein iii. Formed in chain iv. Both the sub units are bounded by a membrane Ribosome may also be called i. Microsome ii. Dictyosome iii. Ribonucleoprotein iv. Oxysome Acid hydrolase is found in lysosomes. The functional unit in the synthesis of protein is polysome. Ribosome that occurs exclusively in mitochondria is 55 S. The marker enzyme of lysosome is acid phosphatase. Ribosome of bacteria, mitochondria, prokaryotes (Nostoc) and chloroplast are of 70S type. The color of rose petals is due to water soluble pigments present in the vacuoles. Which is not true about sphaerosomes? i. Arose from ER ii. Related to fat iii. Single membrane bound structure iv. Involved in photorespiration Peroxisomes are rich in oxidative enzymes. Tonoplast is covering layer of tonoplast. In which of the following would you expect to find glyoxysomes? i. Endosperm of wheat ii. Endosperm of Castor iii. Palisade cells of leaf iv. Root hairs Hyaloplasm of vacuole contains water and minerals. Which of the following has a single unit membrane? i. Vacuole ii. Lysosome iii. Golgi complex iv. Peroxisome Which one of the following isn’t a cell inclusion? i. Crystal ii. Vacuole iii. Starch iv. Fat droplets The osmotic expansion of a cell kept in water is chiefly regulated by vacuoles. Which of the following parts of the cell is non-living? i. Centrioles ii. Vacuoles iii. Ribosome iv. Mitochondria The fluid part of cell called cell sap is the non-living contents of the vacuole of cell. Which organelles is devoid of DNA yet is capable of duplication? Centriole Number of protofilaments in microtubule is 13. The principal protein of cilia and flagella is tubulin. In flagellar membrane which enzyme catalyzes ATP i. Cytoplasmic dynein ii. Asconic dynein iii. Kinesis iv. Myosin The number of microtubules in a flagellum including those sharing these protofilaments with each other is 20. The nucleoplasm is continuous with the cytoplasm of a cell through nuclear pores. Karyolymph is a nuclear sap. The nuclear spindle consists of three types of fibres. Karyology is the study of nucleus. Spindle chromosomes have kinetochore. The telomeres of eukaryotic chromosomes consist of short sequence of guanine rich repeats. In nucleoplasm, a conspicuous body of spherical shape attached to a particular chromosome on a definite position is called nucleolus. Who showed that the nuclear membrane has many pores or circular structures or annuli? Callan and Tomlin Minimum haploid numbers of chromosomes in plant kingdom is 2. The part which doesn’t take strain amongst the following is i. Chromatid ii. Centromere iii. Chromatin iv. Chromomere Which of the following are used to define the karyotype of a species? i. Number of chromosomes ii. Chromosomes length iii. Position of centromere iv. All of the above Cells obtained from organisms were homogenized and centrifuged. A test indicated that the cells contained glycogen. If you were asked to find out as quickly as possible whether the cells were from a plant or am animal, you would i. Examine the centrifuge for the presence of extracts of chloroplasts ii. Answer immediately that the cells were from a plants source iii. Examine the centrifuge for the presence of extracts of centrioles iv. Answer immediately that the cells were from an animal source Disulphide bonds which act as atomic staples to reinforce the confrontation of proteins are found in endoplasmic reticulum. Cytoskeletal network of a cell is built by a process called dynamic instability. One of the most common enzymes found in peroxisome is catalase. Phragmoplast is i. Cell plate formed by ER and products of dictyosome during cytokinesis ii. Cell membrane formed by ER, Golgi bodies and secretary vesicles during cytokinesis iii. Plastid capable of fragmentation iv. Plastid capable of duplication Green potatoes are toxic due to phytoalexins. The term lipochondria was suggested by Golgi complex. Nucleoid is a nucleus without nuclear membrane and nucleolus or genetic material of prokaryotes. Importance of mitochondria in respiration was first discovered by Michaelis. What is the proportion of lipids in chloroplast? 20-30% Cell is the fundamental structural and functional unit of all living organisms is evidenced by the facts like - i. Anything less than a complete structure of a cell does not ensure independent living ii. Subcellular components can regenerate whole cell iii. A cell arises by fusion of two cell iv. All cells are totipotent Select the right option which relates to Schwann regarding the following statement - I. He reported that cells have a thin outer layer which is today known as plasma membrane II. Cell wall is a unique character of the plant cell • Ill. Body of plants and animals are composed of cells and products of cells i. All are correct ii. Only Ill is correct iii. II and Ill are correct iv. All are incorrect Organization of a cell has not been achieved in Bacteriophage Which of the following statements concerning prokaryotes is/are true? i. Because prokaryotes do not contain organelles, they cannot photosynthesize or carry out cellular respiration ii. Prokaryotes have no chromosomes and therefore lack DNA iii. Prokaryotic flagella are similar in structure to eukaryotic flagella iv. None of the above All of the following statements are correct about plasmids except - i. They are extrachromosomal DNA ii. They are smaller, circular, double stranded naked DNA that confer certain unique phenotypic characters to some bacteria like resistance to antibiotics iii. They are used in genetic engineering iv. It helps in the replication of nucleoid Which of the following is not true about fimbriae? i. They are composed of protein ii. They are small bristle like fibers sprouting out of the bacterial cell iii. They may be used for attach iv. They form pellicle
I. It is the extension of plasma membrane into the cytoplasm
II. It helps in cell wall formation, DNA replication, helps in respiration, secretion processes, increases the surface area of plasma membrane and enzymatic contents, also helps in cytokinesis. III. It is the characteristic of bacterial cells. The above feature is attributed to bacterial- i. Nucleoid ii. Plasmid iii. Mesosome iv. Pilus Bacteria can be classified into 2 groups on the basis of i. Differences in the cell envelopes ii. The manner in which they respond to the staining procedure developed by Gram iii. Occurrence of pili iv. Both (i) and (ii) Which of the following statement is false for prokaryotic cell inclusions? i. These are storage granules in the cytoplasm ii. They are membranous iii. Phosphate granules, cyanophycean granules and glycogen granules are the examples of cell inclusions iv. Gas vacuole is found in B.G.A and purple and green photosynthetic bacteria If you removed the fimbriae from a bacterial cell, which of the following would you expect to happen? i. The bacteria could no longer swim ii. The bacteria would not adhere to the host tissue iii. Transportation of molecules across the membrane would stop iv. The shape of bacteria would change The detailed structure of the membrane was studied in the 1950s only after the advent of the - i. Phase contrast microscope ii. Electrophoresis iii. Electron microscope iv. DNA probes The best material for the study of structure of cell membrane is - i. RBC of human ii. RBC of frog iii. Kidney cell iv. Muscle cell Biochemical investigation reveals that the cell membrane is composed of- i. Proteins + Lipids only ii. Carbohydrate+ Protein only iii. Carbohydrate+ Lipids+ Proteins iv. Carbohydrate+ lipids only
Why tail of lipids in the membrane are towards inner part?
i. The tail is non polar hydrocarbon and so protected within an aqueous environment ii. The tail is polar hydrocarbon and so is protected from aqueous environment iii. The non-polar or hydrophobic hydrocarbon tails of lipid, being on inner side ensures their protection from aqueous environment iv. The tail is hydrophilic so it tends to be located in the aqueous inner side of membrane According to the modern concept, cell membrane is - i. Solid ii. Quasifluid iii. Fluid iv. Solidified sheath Integral cell membrane proteins - i. Are partially embedded in lipid layers ii. Are completely embedded in lipid layers iii. Show lateral but not vertical movements within bilayer of lipid iv. All Fluid nature of membrane is able to explain - i. Cell growth, cell division ii. Secretion and permeation of various substances across membrane iii. Formation of intercellular junction iv. All Which statement about the plant cell wall is not true? i. Its principal chemical composition is polysaccharide ii. It completely isolates adjacent cells iii. It is rigid iv. All The innermost portion of a mature plant cell wall is the - i. Primary cell wall ii. Plasma membrane iii. Secondary cell wall iv. Plasmodesmata Choose the false statement- i. The 1° cell wall of young cell is capable of growth ii. Growth of 1° cell wall diminishes as the cell matures iii. 1° cell wall is very non-elastic iv. Secondary cell wall is thicker, stronger and positioned between the 1~ cell wall and cell membrane A cell, which is very active in the synthesis and secretion of proteins, would be expected to have - i. Equal amount of RER and SER ii. More SER than RER iii. More RER than SER iv. More GB and no RER The concentration of a number of ions and other materials is higher in vacuoles than those in cytoplasm. Why? i. Tonoplast has a number of active transport system that pumps ions into vacuole from cytoplasm ii. Through osmosis, a large number of ions go continuously to vacuole from cytoplasm iii. Cytoplasmic ions enter the vacuole through osmotic flow of water iv. Vacuole is always engaged in the hydrolysis of salts into their ions Which of the following statement is incorrect? i. Mitochondria, unless specifically stained are not easily visible under the microscope ii. Physiological activity of cells determines the number of mitochondria per cell iii. Mitochondrion, a power house of cell has DNA, RNA, ribosomes and enzyme. So, it can survive outside the cell iv. Mitochondria divide by fission Both the membranes of a mitochondrion are i. Structurally different but functionally similar ii. Structurally similar but functionally different iii. Structurally as well as functionally different iv. Structurally as well as functionally similar Plastids are found in - i. All animal cells ii. Some animal cells iii. All plant cells iv. All plants cells and euglenoids Chloroplasts contain - i. All types of pigments ii. Chl + Carotenoids iii. Chl + Carotene+ anthocyanine iv. Only chl
Majority of the chloroplast of the green plants are found in -
i. Mesophylls of leaves ii. Bundle sheath of leaf iii. Vascular bundle of leaf iv. Mid ribs of leaf The number of chloroplasts in each mesophyll is 20-40 Which of the following is correct about the outer and inner membrane of chloroplast? i. Less permeable and more permeable respectively ii. More permeable and less permeable respectively iii. Both are equally permeable iv. Both are equally impermeable The cellular structures that are almost like centrioles are - i. Basal bodies ii. Microfilaments iii. Microtubules iv. Centromeres The core of cilium or flagellum, composed of microtubules and their associated proteins is called - i. Blepharoplast ii. Axoneme iii. Microfilament iv. Tubulin Which of the following statement is false? i. Both the centrioles in a centrosome lie perpendicular to each other ii. Central proteinaceous hub is missing in a centriole iii. Each centriole has an organization like that of a cartwheel. iv. Centrosome usually contains 2 cylindrical centrioles
Number of nucleolus in a nucleus is –
i. Only one ii. Many iii. Dependent on number of SAT-chromosome iv. One or more Material of the nucleus is stained by Basic dye For the study of structure of nucleus, the best cell is - i. Cell in the interphase ii. Cell in the late prophase iii. Cell in the divisional phase iv. Cell in the meiotic phase Actively functional nucleus shows- i. Large nucleolus, diffused chromatin and more nuclear pores ii. Large nucleolus, diffused chromatin and no nuclear pores iii. Large nucleolus, compact chromatin and many pores iv. No nucleolus, diffused chromatin and small nuclear pores The nuclear pores are the passage for the movement of certain materials between the nucleus and cytoplasm in the direction. Which one is correct about materials in their respective direction i. Proteins, enzymes into the nucleus ii. Ribosomal components out of the nucleus iii. mRNA out of the nucleus iv. All The total length of DNA molecules of 46 chromosomes in a human cell is about _____, whereas a typical is 10 min length - i. 2 mm ii. 2 cm iii. 0.2 mm iv. 2m The term "nuclear envelope" is more correct than the term "nuclear membrane" because i. the enclosure has pores which membranes do not ii. the enclosure is made up of two membranes iii. the chemical composition is inconsistent with cellular membranes iv. None of the above. The two terms are perfect synonyms. Passage through pores in the nuclear envelope is restricted primarily to i. proteins, RNA, and protein-RNA complexes ii. lipids and glycolipids iii. DNA and RNA iv. RNA and protein-carbohydrate complexes Cell fractionation is the most appropriate procedure for preparing for study. i. isolated cells which are normally found tightly attached to neighboring cells ii. cells without a functional cytoskeleton iii. isolated organelles iv. the basic macromolecules The cell membranes of adjacent cells are fused at i. macula adherens ii. zonula adherens iii. zonula occludens iv. nexus The Golgi complex plays a major role: i. in digesting proteins and carbohydrates ii. as energy transferring organelles iii. in post translational modification of proteins and glycosidation of lipids iv. in trapping the light and transforming it into chemical energy The most abundant intracellular cation is K+. The solid linear cytoskeletal elements having a diameter of 6 nm and made up of a single type of monomers are known as i. Microtubules ii. Microfilaments iii. Intermediate filaments iv. Lamina Why is a capsule advantageous to a bacterium? i. It allows the bacterium to attach to the surface ii. It protects the bacterium from desiccation iii. It provides means of locomotion iv. It allows bacterium to 'hide' from host's immune system The filament in flagellum can rotate by 360° Which one of the following is not an inclusion body found in prokaryotes? i. Glycogen granule ii. Polysome iii. Phosphate granule iv. Cyanophycean granule Which of the following cells lacks cytoskeleton? i. Eukaryotic plant cell ii. Prokaryotic bacterial cells iii. Both (i) and (ii) iv. Prokaryotic cells and eukaryotic animal cells To study the ability to secrete a specific protein, cells were homogenized mechanically and organelles were separated by centrifugation. Which organelle should be used for further investigation? Microsome In a resting nucleus, centromeres appear as - i. Euchromatin ii. facultative heterochromatin iii. constitutive heterochromatin iv. nucleolus Biochemical analysis of a cell fraction revealed no carbohydrates, 1 % RNA, 0.2% DNA, 40% lipids and 60% proteins. It may possibly be a pure - i. Plasma membrane fraction ii. nuclear fraction iii. microsomal fraction iv. mitochondrial fraction The fluidity of membranes in a plant in cold weather may be maintained by i. Increasing the number of phospholipids with unsaturated hydrocarbon tails. ii. Increasing the proportion of integral proteins. iii. Decreasing concentration of cholesterol in membrane iv. Increasing the number of phospholipids with saturated hydrocarbon tails Cell organelle that divides intracellular space of a cell into two compartments luminal space and extra luminal space i. May have ribosomes attached by its smaller subunit ii. Performs the function of packaging materials iii. Shows a distinct polarity and is situated near the nucleus iv. Is composed of tiny tubular structures scattered in the cytoplasm The genome of onion has 8 chromosomes (n = 8). In a root tip cell undergoing anaphase the number of chromosomes will be 32 The largest plastid is i. Chloroplast ii. Chromoplast iii. Aleuroplast iv. Amyloplast