GENERAL BIOLOGY 1

Laboratory Worksheet 5: Photosynthesis

2nd Period, A.Y. 2023-2024

Name: _____________________________ Section: __________________________

Score:
Teacher: ___________________________ Date Performed: __________________

A. Light as a requirement for photosynthesis.

Rubric for the drawing (3 points each x 2= 6 points)
/35
Criteria 3 points 2 points 1 point
Color
The color of the drawing reflects
that of the actual result.
Completeness
The student satisfies all The student satisfies 2 The student satisfies only 1
The drawing shows the entire image
the three criteria listed of the criteria listed of the criteria listed
of the leaf given.
Accuracy
The drawing shows the correct result
of the experiment.

Plectranthus scutellarioides Leaf Plectranthus scutellarioides Leaf

(COVERED) (EXPOSED)
B. Carbon dioxide as a requirement for photosynthesis. (3 points each x 3= 9 points)

Rubric
3 points-Observation is correct and explanation is clear with correct supporting details.
2 points- Observation is correct and explanation is somewhat clear with few correct
supporting details.
1 point- Observation is incorrect.

Test tube Observations and Explanations

A
-Blown phenol red
- Without Hydrilla verticillata
B
-Blown phenol red
-With Hydrilla verticillata
C
-Unblown phenol red

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Laboratory Worksheet 5: Photosynthesis
Senior High School Department
2nd Period/ A.Y. 2023-2024
 - With Hydrilla verticillata

C. Separation of chloroplast pigments by paper chromatography

Rubric for the Separation of chloroplast pigments drawing (3 points)

Criteria 3 points 2 points 1 point
Color
The color of the drawing reflects
that of the actual result.
Completeness
The student satisfies all The student satisfies 2 The student satisfies only 1
The drawing shows all the pigments
the three criteria listed of the criteria listed of the criteria listed
seen.
Accuracy
The drawing shows the correct result
of the experiment.

(Just draw your group’s assigned leaf)

Green leaf Variegated leaf

Rubric for the Analysis of Pigment Bands

Criteria 4 3 2 1
Data The data presented in Some data are missing More than half of the Only few data are
Gathering each row of the table is in each row of the data are missing in presented in the
complete. table. the table. table.
Calculations All calculated Rf values Some calculated Rf More than half of the Only few calculated
are correct. values are incorrect. calculated Rf values Rf values are correct.
are incorrect.
Accuracy All calculated Rf values Some calculated Rf More than half of the Only few of the
are comparable to the values are far from the calculated Rf values calculated Rf values
standard. standard. are far from the are comparable to
standard. the standard.

Analysis of Pigment Bands (12 points)

Band Distance Band Color Pigment Name Rf value Rf value
Number (mm) (calculated) (standard)

Distance travelled by solvent front: _______mm (1 point)

Side Questions:
1. How many color bands did you observe? _________ (1 point)

2. a. Which color band travelled the farthest? _____________________ (1 point)

b. The least? ____________________ (1 point)
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Laboratory Worksheet 5: Photosynthesis
Senior High School Department
2nd Period/ A.Y. 2023-2024
 c. What does the distance travelled say about the pigment? (1 point)
__________________________________________________________________________________________

Submission Note:
1. Save the document in x.pdf format with the file name:
Section_Group#_ ACTIVITY# (ex. STEM12A1_Group4_Activity04)

2. Upload the file to the corresponding module under the submission page in the CANVAS.

Table of Contributions: Indicate the work contribution of the group members below. Member with no
participation will be graded zero (0).

Name Contribution Remarks

1.
2.
3.
4.
5.

COPYRIGHT OF THIS MATERIAL (except video links) BELONGS TO:

Biology Department
De La Salle University

NO PART OF THIS MATERIAL MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM OR BY ANY MEANS --- ELECTRONICALLY OR
MECHANICALLY

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Laboratory Worksheet 5: Photosynthesis
Senior High School Department
2nd Period/ A.Y. 2023-2024