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Protein synthesis: Transcription and translation

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Transcription

Define transcription

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Distinguish between sense and antisense strands

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Outline the role of RNA polymerase in the process of transcription

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Convert the following DNA sequence into an RNA transcript

T A C A A A T T C G T A C T G C A C T C C G G A A C A A C T

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Translation

Define translation

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Describe the function of the genetic code (including degeneracy)

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Identifying the key components of the process of translation

M ………………………………………………………………………………………………

R ………………………………………………………………………………………………

C ………………………………………………………………………………………………

A ………………………………………………………………………………………………

T ………………………………………………………………………………………………

A ………………………………………………………………………………………………

P ………………………………………………………………………………………………

P ……………………………………………………………………………………………… Hint: Mr Cat App

Summarise the process of translation

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Use the genetic code to convert the following DNA
sequence into a polypeptide sequence

TAC AAA TTC GTA CTG CAC TCC GGA ACA ACT

RNA: ………………………………………………………………….

Protein: ……………………………………………………………..

Outline the effect of the following mutations:

GTA → GTT: ………………………………………………………

TCC → TCT: ………………………………………………………

Describe the different types of point mutations

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Outline the significance of a frameshift mutation

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Use the following image to explain how the universality of the genetic code allows for gene transfer

Insulin gene
Human Cell
Grow in Extract
Recombinant Transgenic culture insulin
Plasmid bacteria
Bacteria Plasmid

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