the value in the result window and record it in mg/L (or

Instruction Manual SPECIFICATIONS ppm) of Phenols.

Range 0 to 1.00 mg/L (ppm) as Phenols LR
0.5 to 5.0 mg/L (ppm) as Phenols HR
Smallest Increment 0.02 mg/L (ppm) Phenols LR
HI 3864 0.1 mg/L (ppm) Phenols HR
Analysis Method Colorimetric
Phenols Sample Size 10 mL
• Fill each glass vial with 10 mL of
sample, up to the mark. 10 mL
Number of Tests 100 • If the color of the sample is too intense to make a color
Test Kit Case Dimensions 235x175x115 mm (9.2x6.9x4.5")
• Insert one of them into the left hand opening of the
checker disc. This is the blank.
match with the mirror installed, then remove from the
Shipping Weight 573 g (20.2 oz.) checker disc:
i. the glass vials
ii. the LR disc
SIGNIFICANCE AND USE
iii. the mirror and its holder.
Phenols are widely used in pharmaceuticals, dyes and indica-
tors and as general disinfectants. They may occur in household • Install the HR (0.5÷5 mg/
and industrial wastewaters and in natural waters; they can L) disc and replace the glass
also enter potable water supplies and chlorination of such vials in the checker disc.
waters results in malodorous chlorophenol products that are
www.hannainst.com • Add to the other
detectable from 0.001 mg/L (1 ppb). The HANNA colorimetric
method determines phenol and all ortho and para substituted glass vial 1 • Hold the checker disc so that a
phenols. Since substitution generally lowers the response, the packet of HI light source illuminates the
readable value obtained by this method is the minimum 3864A-0 Re- samples from the back windows.
concentration of phenolic compounds present. Natural water agent. Replace • Rotate the disc while looking at
usually contains less than 0.001 mg/L (ppb) of phenols, but the cap and the color test windows and stop
sometimes values up to 0.02 mg/L (20 ppb) also occur. mix. when you find the color match.
Dear Customer, Since the concentration of phenols in wastewater is generally • Add 1 packet of HI 3864B-0 Reagent. Read the value in the result
Thank you for choosing a Hanna Product. subjected to biological and chemical degradation, preserve • Start the waiting time of 10 minutes. window and record it in mg/L (or ppm) of Phenols.
samples in the refrigerator and analyze within 4 hours after Replace the cap and
Please read the instructions carefully before using the For best results: Intensely colored samples will make the color
collection.
chemical test kit. It will provide you with the necessary shake the vial until the matching determination difficult and they should be
Note: mg/L is equivalent to ppm (parts per million), ppb are powder has totally dis-
information for correct use of the kit. adequately treated before performing the test. Sus-
parts per billion. solved. The solution will
Remove the chemical test kit from the packing material and pended matter in large amounts should be removed
examine it carefully to make sure that no damage has turn a color yellow to or- by prior filtration.
occurred during shipping. If there is any noticeable dam- CHEMICAL REACTION ange. This is the reacted
sample. Caution: Ultraviolet radiation may cause fading of colors.
age, notify your Dealer or the nearest Hanna office Phenolic compounds react to form a yellow to orange dye with When not in use, keep the disc protected from light,
immediately. • Remove the cap and in-
4-amino antipyrine in alkaline solution buffered at pH about in a cool and dry place.
Each kit is supplied with: 10. The absorbance of this colored product is proportional to sert the reacted sample
PRINTED IN ITALY

• HI 3864A-0 Reagent, packets (100 pcs); the concentration of phenols present in the aqueous sample. into the right hand open- REFERENCES
ing of the checker disc.
• HI 3864B-0 Reagent, packets (100 pcs); Standard Methods for the Examination of Water and Waste-
INSTRUCTIONS • Hold the checker disc so that
• 1 checker disc (with 2 discs, LR and HR); water, 18th edition, 1992
a light source illuminates the
• 2 glass vials with caps; READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT samples from the top. HEALTH AND SAFETY
11/99

• 1 mirror. • Verify if the mirror and the LR (0÷1 mg/L) disc are • After the 10 minutes have The chemicals contained in this kit may be hazardous if
Note: Any damaged or defective item must be returned in already pre-installed in the checker. If not, install both passed, rotate the disc while looking at the color test
ISTR3864

improperly handled. Read Health and Safety Data Sheet

its original packing materials. as shown in the figure. windows and stop when you find the color match. Read
before performing this test.