22/2/24, 17:10 PhysioEx Exercise 8 Activity 4

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion
Activity 4: Assessing Lipase Digestion of Fat
Name: ejercicio 8.4
Date: 22 February 2024
Session ID: session-3c727274-ca9b-b3eb-57c3-5a140185bd06

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Which of the following is/are true of bile?

You correctly answered: It works by a physical process.

2 The substrate used in this simulation is

You correctly answered: vegetable oil.

3 When fatty acids are liberated by lipase, the pH

You correctly answered: decreases.

4 One of the products of the chemical digestion of lipids is

You correctly answered: fatty acids.

Experiment Results
Predict Question
1 Predict Question: Which tube do you think will have the highest lipase activity?

Your answer: tube 6 (lipase, vegetable oil, bile salts, pH 9.0 buffer).

Stop & Think Questions

1 What does the pH measure?

You correctly answered: both lipase activity and fatty acid release.

2 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile
salts, pH 2.0 buffer)?

You correctly answered: The pH is already very low, so a decrease in pH might be difficult
to detect.

Experiment Data
Tube Reagent Reagent Reagent Reagent Time Temp pH
No 1 2 3 4 (min) (°C) i
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Vegetable pH 7.0
1 Lipase Bile salts 60 37 6.21
Oil Buffer
Vegetable Deionized pH 7.0
2 Lipase 60 37 7.00
Oil Water Buffer
Deionized pH 9.0
3 Lipase Bile salts 60 37 9.00
Water Buffer
Deionized Vegetable pH 7.0
4 Bile salts 60 37 7.00
Water Oil Buffer
Vegetable pH 2.0
5 Lipase Bile salts 60 37 2.00
Oil Buffer
Vegetable pH 9.0
6 Lipase Bile salts 60 37 9.00
Oil Buffer

Post-lab Quiz Results

You scored 75% by answering 3 out of 4 questions correctly.

1 What is the product of lipase hydrolysis?

You correctly answered: fatty acids.

2 From your results, which pH is ideal for pancreatic lipase digestion?

You correctly answered: pH 7.0.

3 Which tube confirms that there is no lipase in bile salts or vegetable oil?

You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer).

4 Where in the body does pancreatic lipase hydrolyze triglycerides?

Your answer: mouth.

Correct answer: small intestine.

Review Sheet Results

1 Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts,
pH 2.0 buffer).

You did not answer this question.

2 Which tube had the highest lipase activity? How well did the results compare with your
prediction? Discuss possible reasons why it may or may not have matched.

Your answer:

No se predijo correctamente que el tubo donde a haber mayor actividad de la lipasa iba a
ser en el tubo 1, ya que en este,comparándolo con los tubos 5 y 6 donde se varía el pH es
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donde más se redujo el pH en comparación y por lo tanto donde mejor funciona la lipasa
es a pH 7.

3 Explain why pancreatic lipase would be active in both the mouth and the pancreas.

Your answer:

Sería activa en estos dos sitios porque tiene un pH óptimo para su funcionamiento, es
decir, tienen un pH de 7.

4 Describe the process of bile emulsification of lipids and how it improves lipase activity.

Your answer:

La bilis funciona como un detergente, es decir, cuando son liberadas en el intestino

delgado emulsionan los cúmulos de lípido, los separan y por lo tanto las lipasas tendrían
una mayor superficie de actividad para actuar.

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