Are you struggling with writing your PhD thesis on Chlamydia Trachomatis? You're not alone.

Crafting a thesis on such a complex and intricate subject can be incredibly challenging. From
conducting thorough research to organizing your findings and articulating them effectively, the
process can be daunting and time-consuming.

Chlamydia Trachomatis is a bacterium that can cause a range of diseases in humans, making it a
significant topic of study in various fields, including microbiology, epidemiology, and public health.
As such, a thorough understanding of the subject matter is essential for producing a high-quality
thesis that contributes meaningfully to the academic discourse.

However, many students find themselves overwhelmed by the sheer volume of information available
on Chlamydia Trachomatis and struggle to synthesize it into a coherent and compelling thesis.
Additionally, the rigorous standards and expectations of the academic community can add further
pressure, leaving students feeling stressed and uncertain about their ability to produce work of
sufficient quality.

Fortunately, there is a solution. If you're finding it difficult to write your PhD thesis on Chlamydia
Trachomatis, consider seeking assistance from ⇒ HelpWriting.net ⇔. Our team of experienced
academic writers specializes in a wide range of subjects, including microbiology and epidemiology,
and can provide the expertise and support you need to produce a standout thesis.

By ordering from ⇒ HelpWriting.net ⇔, you can rest assured that your thesis will be in the hands
of professionals who understand the intricacies of the subject matter and are committed to helping
you succeed. Whether you need assistance with research, writing, or editing, our team is here to help
you every step of the way.

Don't let the challenges of writing a PhD thesis on Chlamydia Trachomatis hold you back. Order
from ⇒ HelpWriting.net ⇔ today and take the first step towards achieving your academic goals.
Savina A, Jancic C, Hugues S, Guermonprez P, Vargas P, Moura IC, et al. It also causes trachoma
eye disease, a leading cause of blindness. Tissue banking Tissue banking EVALUATION OF
TESTICULAR PAIN.pdf EVALUATION OF TESTICULAR PAIN.pdf Ccpp Ccpp Crimson
Publishers-Antibiotic Sensitivity Pattern of Bacteria Isolated from S. Data Availability Statement The
raw data supporting the conclusions of this article will be made available by the authors, without
undue reservation. After in vivo staining, cells were washed 3 times with PBS, fixed with 2% PFA,
and analyzed by FACS. DCs are the most potent antigen-presenting cells of the immune system and
tightly regulate their endocytic system to process and present antigens efficiently onto MHC
molecules ( 30 ). The late endocytic Rab39a GTPase regulates the interaction between multivesicular
bodies and chlamydial inclusions. Valdivia RH. Chlamydia effector proteins and new insights into
chlamydial cellular microbiology. Qn1: Retrieving a paper Qn2a: How many strains and types of
Chlamydia are there. Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a
Rab14-mediated transport required for bacterial development and replication. Insets show a
chlamydial inclusion magnification for each marker. Houde M, Bertholet S, Gagnon E, Brunet S,
Goyette G, Laplante A, et al. Available at:. Google Scholar 2. Menon S, Timms P, Allan JA,
Alexander K, Rombauts L, Horner P, et al. To browse Academia.edu and the wider internet faster
and more securely, please take a few seconds to upgrade your browser. MHC-I molecules expression
level at the cell surface was significantly reduced in infected JAWS-II DCs ( Figures 4C, D ).
Barrow Motor Ability Test - TEST, MEASUREMENT AND EVALUATION IN PHYSICAL EDUC.
Key words: Chlamydia trachomatis, McCoy cell, Inclusion body. He was awarded the DAAD
Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-
Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Endogenous Rab14,
Rab4, Rab22a and Rab11a proteins were detected by indirect immunofluorescence using primary
antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Acinetobacter
Acinetobacter raza3182 Giardiasis Giardiasis Anup Bajracharya Chlamydia Chlamydia Dr. Rakesh
Prasad Sah Cytomegalovirus Cytomegalovirus Vishal Ramteke More Related Content What's hot
Strongyloides stercoralis Strongyloides stercoralis Aruna Rani Behera GIARDIA LAMBLIA
GIARDIA LAMBLIA Suraj Dhara Chlamydia; bacterial genus Chlamydia; bacterial genus Sayed
Mamun 20. A) Representative FACS histograms show 7-AAD MFI corresponding to unstained
(grey), non-infected (red line) and C. Immunoblotting JAWS-II DCs non-infected or infected with
C. Cebrian I, Croce C, Guerrero NA, Blanchard N, Mayorga LS. Additionally, we performed another
quantitative analysis whereby each inclusion was transversely crossed with a line, then five points
corresponding to the sections cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3),
inclusion membrane (P4), and cytoplasm (P5) were selected to obtain an intensity histogram. Dr.
Hala Al-Daghistani. Chlamydia is a genus of pathogenic bacteria that are obligate intracellular
parasites of mammals and birds. Eukaryotes (47 Key Differences) Animal Cell: Structure, Parts,
Functions, Labeled Diagram. Inclusion Forming Units Inclusion Forming Units (IFUs) per microliter
to quantify EBs harvested from infected DCs were assessed as previously described ( 10 ). This
interaction between the chlamydial inclusion and recycling endosomes directly impacts the proper
MHC-I intracellular trafficking and cross-presentation ability of infected DCs. Finally, to evaluate
EB production, JAWS-II DCs were infected with C. However, the cell culture technique is
technically difficult and requires 3-7 days to obtain a result, special.
On one side, C. trachomatis has developed multiple strategies to evade the immune system. Bars
represent 10 um. (C) Representative FACS histograms showing the fluorescence intensity of
unstained (grey), non-infected (black line), and infected (red line) cells. Osborne DG, Piotrowski JT,
Dick CJ, Zhang JS, Billadeau DD. Volume 4: The Bacteroidetes, Spirochaetes, Tenericutes
(Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes,
Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. Cebrian I, Visentin G, Blanchard
N, Jouve M, Bobard A, Moita C, et al. This compartment emerges as an important hub during the
intracellular transport of exogenous antigens in DCs, particularly during MHC-I cross-presentation.
Armed Forces Research Institute of Medical Sciences160. Chlamydia trachomatis causes various
types of genital, eye and lymph node infections. Upon completion of this content, the learner will be
able to: Describe the epidemiology of chlamydial infection in the U.S. Describe the pathogenesis of
Chlamydia trachomatis. Ascaris lumbricoides and other Gastrointestinal Helminthic Parasites among
Qe. The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and
chlamydial inclusions. Alternatively activated macrophages are host cells for Chlamydia trachomatis
and reverse anti-chlamydial classically activated macrophages. Setting: Armed Forces Research
Institute of Medical Sciences (AFRIMS). It also causes trachoma eye disease, a leading cause of
blindness. Chlamydia trachomatis is known to cause urethritis, epididymis, proctitis, cervicitis. To
browse Academia.edu and the wider internet faster and more securely, please take a few seconds to
upgrade your browser. We also assessed total intracellular OVA (non-fluorescent and fluorescent
DQ-OVA molecules) by staining with an anti-OVA antibody coupled to Alexa 647. For this purpose,
we incubated non-infected JAWS-II DCs and cells infected with C. The complete list of antibodies
and reagents, with catalog number and references, is provided as Supplementary Table 1. The first
page of the PDF of this article appears above. Line graphs show the intensity histogram of each Rab.
Altogether, these data indicate that the MHC-I intracellular transport is significantly compromised in
DCs when these cells are infected by the bacterium C. Differential regulation of inflammatory
cytokine secretion by human dendritic cells upon Chlamydia trachomatis infection. Persistent
chlamydiae: From cell culture to a paradigm for chlamydial pathogenesis. We confirmed and
quantified this finding by flow cytometry analysis measuring the MFI of anti-H-2K b staining. Flow
Cytometry Analysis To determine the infection rate of JAWS-II DCs, these cells were infected or not
with GFP- C. EBs are the infective form that can survive outside the host cell. Rab11-family of
interacting protein 2 associates with chlamydial inclusions through its Rab-binding domain and
promotes bacterial multiplication. Asymptomatic Bacteriuria among Pregnant Women Attending
Antenatal: Evaluation. He was awarded the DAAD Research Grant to conduct part of his Ph.D.
research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research
Saarland (HIPS), Saarbrucken, Germany.
Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport
required for bacterial development and replication. You will be able to get a quick price and instant
permission to reuse the content in many different ways. BMDCs were generated after 9 to 14 days of
bone marrow cell culture in GM-CSF-containing medium, as previously described ( 41 ). This fact
may provoke several complications, such as pelvic inflammatory disease, fallopian tube obstruction,
ectopic pregnancy, and infertility. Interestingly, similar results were obtained when primary bone
marrow-derived DCs (BMDCs) were infected during 24 h and soluble OVA cross-presentation was
evaluated. Once internalized, the bacterium actively modified the phagosomal membrane to generate
a protective vacuole, the inclusion that diverts from the phagocytic pathway and avoids lysosomal
degradation. Cebrian I, Croce C, Guerrero NA, Blanchard N, Mayorga LS. Insets show a
magnification of the chlamydial inclusion. Genus: Chlamydia C. trachomatis - Urogenital infections,
trachoma, conjunctivitis, pneumonia and lymphogranuloma venerium (LGV) Genus: Chlamydophilia
C. To evaluate this hypothesis, we used a flow cytometry-based approach previously described ( 35 )
and we measured MHC-I recycling in JAWS-II DCs. Confocal microscopy and flow cytometry
approaches show the loss of MHC-I molecules at the cell surface and the perinuclear recycling center
after infection. Rab Proteins Involved in Endocytic Recycling Are Recruited to the Chlamydial
Inclusion To characterize the interaction between the chlamydial inclusion and recycling endosomes,
we next evaluated the intracellular distribution of different Rab proteins that regulate endocytic
recycling transport before and after C. Upon completion of this content, the learner will be able to:
Describe the epidemiology of chlamydial infection in the U.S. Describe the pathogenesis of
Chlamydia trachomatis. Beyond differences in the pattern or intensity of Rab associated with the
inclusion, these proteins confer the bacterium ability to interact with the endocytic recycling
compartment. Inhibition of indoleamine 2,3-dioxygenase activity by levo-1-methyl tryptophan
blocks gamma interferon-induced Chlamydia trachomatis persistence in human epithelial cells.
Subrata Roy Psychedelic Treatment Planning: Opportunities in Integrated Care Psychedelic
Treatment Planning: Opportunities in Integrated Care Brian Peacock ANTI-HYPERTENSIVE
DRUG - RAUWOLFIA ROOT. Nevertheless, even though MHC-I trafficking to the plasma
membrane was affected after C. By using a wide repertoire of bioinformatics tools, they can help us
to draw particular conclusions about species and general ones about evolution. We also assessed total
intracellular OVA (non-fluorescent and fluorescent DQ-OVA molecules) by staining with an anti-
OVA antibody coupled to Alexa 647. Andreas Schleicher - 20 Feb 2024 - How pop music, podcasts,
and Tik Tok are i. The first page of the PDF of this article appears above. Flow Cytometry Analysis
To determine the infection rate of JAWS-II DCs, these cells were infected or not with GFP- C. Sagar
has more than ten years of experience in blogging, content writing, and SEO. Schust DJ, Ibana JA,
Sugimoto J, Nagamatsu T, Greene SJ, Quayle AJ. He is the Research Head of the Department of
Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal.
TLR signals induce phagosomal MHC-I delivery from the endosomal recycling compartment to
allow cross-presentation. Insets show a magnification of the chlamydial inclusion. While Rab22a and
Rab11a displayed less pronounced and discontinuous recruitment in patches-like patterns, as shown
in Figures 3K, O, respectively. Rab proteins and the compartmentalization of the endosomal system.
Work-role of Radiation Therapists in the Consequences of Adaptive Radiotherap.
Eukaryotes (47 Key Differences) Animal Cell: Structure, Parts, Functions, Labeled Diagram. Lad SP,
Yang G, Scott DA, Wang G, Nair P, Mathison J, et al. Notably, all four Rabs localized around the
chlamydial inclusion, to a greater or lesser extent, upon DC infection. Chlamydia trachomatis is
known to cause urethritis, epididymis, proctitis, cervicitis. What happened. One of the mares ( 9
months preg) aborts All horses were vaccinated with a 4-way vaccine last May. Several chlamydial
proteins are presented by MHC-I on infected cells, such as CT442 and CT529, becoming good
candidates for vaccine development. Targeting eukaryotic Rab proteins: A smart strategy for
chlamydial survival and replication. Valdivia RH. Chlamydia effector proteins and new insights into
chlamydial cellular microbiology. However, when environmental conditions are disadvantageous, C.
A total of 28 specimens in our laboratory diagnostic service included 20 urethral, 1 conjunctiva and 7
cervical. These last findings have been questioned and remain controversial ( 22, 23 ). Statistical
Analysis Student’s unpaired t-test, one-way ANOVA, two-way ANOVA, and the Bonferroni, Sidak
or Dunnett post-test were performed as indicated in each experiment. Results Chlamydia trachomatis
Infects JAWS-II DCs and Interacts With Recycling Vesicles C. Nevertheless, even though MHC-I
trafficking to the plasma membrane was affected after C. H-2K b total expression was detected with
mouse anti-H2-Kb antibody (1:1000). Differences in the recycling kinetics of non-infected and C.
Images are representative of three independent experiments and more than 20 images were analyzed
for each endosomal marker. After in vivo staining, cells were washed 3 times with PBS, fixed with
2% PFA, and analyzed by FACS. Chlamydia trachomatis Infection Hampers the Antigen Cross-
Presentation Ability of DCs Because MHC-I recycling is an important feature of antigen cross-
presentation, we aimed to examine if this immune process is jeopardized by chlamydial infections.
Can induce Exo-, and Endo- Can’t induce Exo-, and Endo-. We confirmed and quantified this
finding by flow cytometry analysis measuring the MFI of anti-H-2K b staining. Neisseria by Dr.
Rakesh Prasad Sah Neisseria by Dr. Rakesh Prasad Sah Parasitological diagnostic techniques by
Negash alamin Parasitological diagnostic techniques by Negash alamin Asymptomatic Bacteriuria
among Pregnant Women Attending Antenatal: Evaluation. Altogether, our data provide new
evidence about the hijacking of MHC-I intracellular trafficking in DCs by C. The intracellular
distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-
infected and infected cells. DC Infection by Chlamydia trachomatis Perturbs MHC-I Intracellular
Trafficking Next, we decided to study the intracellular transport of MHC-I molecules in C. SPG
buffer (5X) was added to the supernatant until normalized concentration and EBs were purified as
previously described ( 10 ). This fact may provoke several complications, such as pelvic inflammatory
disease, fallopian tube obstruction, ectopic pregnancy, and infertility. Results: Sexually transmitted
disease laboratory at AFRIMS for diagnosis of Chlamydia trachomatis remain. Each chlamydial
inclusion was transversely crossed by eight diameter lines to obtain an intensity histogram.
Altogether, these data support the concept of a relevant role for recycling compartments during DC
infection by C.
In line with these experiments, we used other approach to investigate if chlamydial infection
modifies cellular ability to degrade antigens. This review discusses the mechanisms and sequence of
events linking Chlamydial infections to carcinogenesis in the female reproductive organs.
Background: Genital infections due to Chlamydia trachomatis is the most common bacterial sexually.
T0 represents the MFI of cells at 0 min, and Tx is the MFI of cells at 10, 20, and 40 min. Unraveling
how intracellular pathogens interfere with MHC-I antigen presentation to avoid clearance by the
immune system will pave the way to a rational design of protective vaccines to halt the worldwide
spreading of these infections. Report Back from San Antonio Breast Cancer Symposium (SABCS)
2023: Spotlight. The table displays the statistical significance of Rab14, Rab4, Rab22a and Rab11a
fluorescence intensity comparing cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3),
inclusion membrane (P4), and cytoplasm (P5) corresponding to panels 3D, 3H, 3L and 3P. Rabiya
Husain Recently uploaded ( 20 ) UniSC Fraser Coast library self-guided tour UniSC Fraser Coast
library self-guided tour Chromatography-Gas chromatography-Principle Chromatography-Gas
chromatography-Principle DISCOURSE: TEXT AS CONNECTED DISCOURSE DISCOURSE:
TEXT AS CONNECTED DISCOURSE ICSE English Literature Class X Handwritten Notes ICSE
English Literature Class X Handwritten Notes 50 D. Cebrian I, Visentin G, Blanchard N, Jouve M,
Bobard A, Moita C, et al. Electron microscopic observations on the effects of penicillin on the
morphology of Chlamydia psittaci. A brief discussion on Cysticercosis and how the affect the human
body. These spherical bacteria are human parasites and live only inside an inclusion (white) in cells.
Beyond differences in the pattern or intensity of Rab associated with the inclusion, these proteins
confer the bacterium ability to interact with the endocytic recycling compartment. The intracellular
distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-
infected and infected cells. Conclusion: Cell culture technique for chlamydia is the diagnostic
method of choice and is essential for. Amphisomal route of MHC Class I cross-presentation in
bacteria-infected dendritic cells. The cross-presentation ability of non-infected and infected JAWS-II
DCs with C. Tudela JG, Capmany A, Romao M, Quintero C, Miserey-Lenkei S, Raposo G, et al.
Additionally, we incubated murine BMDCs with GFP- C. On one side, C. trachomatis has
developed multiple strategies to evade the immune system. Nuclei were stained with DAPI and
chlamydial inclusions were detected with a rabbit anti-CT529 antibody and a secondary anti-rabbit
Cy3-labeled antibody. Setting: Armed Forces Research Institute of Medical Sciences (AFRIMS).
Rab GTPases are recruited to chlamydial inclusions in both a species-dependent and species-
independent manner. The GraphPad Prism 5 software (CA, USA) was used for all analyses.
Phramongkutkloa Hospital Annual Scientific Meeting, 24-26 Nov 2003, poster. Chlamydia
trachomatis as the cause of infectious infertility: Acute, repetitive or persistent long-term infection.
Overcoming the challenges of molecular diagnostics in government health insti. Dirks JAMC, Van
Liere GAFS, Hoebe CJPA, Wolffs P, Dukers-Muijrers NHTM. Armed Forces Research Institute of
Medical Sciences160. Images were analyzed with the ImageJ software (Fiji).
Altogether, these data support the concept of a relevant role for recycling compartments during DC
infection by C. Viability Assay Non-infected JAWS-II DCs or cells infected with C. Andreas
Schleicher - 20 Feb 2024 - How pop music, podcasts, and Tik Tok are i. Several studies in Nigeria
have demonstrated an association between cervical cancer and C. You will be able to get a quick
price and instant permission to reuse the content in many different ways. Implement a Fibonacci
Number Generator A naive Fibonacci number generator Implement an optimized Fibonacci number
generator That remembers results of sub-problems already solved Evaluate Performance. In this way,
a decrease in the cell associated fluorescence intensity was only due to the intracellular degradation
of MHC-I molecules. Identification of a chlamydial protease-like activity factor responsible for the
degradation of host transcription factors. Precisely, characteristic features of chlamydial infections
are persistence and chronicity, bases of the pathological damage to the female reproductive system (
49 ). Differential infection outcome of Chlamydia trachomatis in human blood monocytes and
monocyte-derived dendritic cells. The cross-presentation ability of non-infected and infected JAWS-
II DCs with C. Osborne DG, Piotrowski JT, Dick CJ, Zhang JS, Billadeau DD. You will be able to
get a quick price and instant permission to reuse the content in many different ways. Initially, cell
culture was considered to be a highly sensitivity and specificity. BMDCs were generated after 9 to
14 days of bone marrow cell culture in GM-CSF-containing medium, as previously described ( 41 ).
And, to evaluate the phagocytic capacity of non-infected and infected JAWS-II DCs with C. Sagar
is interested in research on actinobacteria, myxobacteria, and natural products. Endogenous Rab14,
Rab4, Rab22a and Rab11a proteins were detected by indirect immunofluorescence using primary
antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Overcoming the
challenges of molecular diagnostics in government health insti. Genus: Chlamydia C. trachomatis -
Urogenital infections, trachoma, conjunctivitis, pneumonia and lymphogranuloma venerium (LGV)
Genus: Chlamydophilia C. The monolayers are examined by Joneus iodine staining to visualize the
cytoplasmic inclusions under the. Dirks JAMC, Van Liere GAFS, Hoebe CJPA, Wolffs P, Dukers-
Muijrers NHTM. Phramongkutkloa Hospital Annual Scientific Meeting, 24-26 Nov 2003, poster. In
men, this bacterium can cause prostatitis, urethritis, and epididymitis ( 1, 2 ). C. trachomatis has two
morphologically distinct developmental forms: elementary bodies (EBs) and reticulate bodies (RBs).
Regulation of antigen export to the cytosol during cross-presentation. Tudela JG, Capmany A,
Romao M, Quintero C, Miserey-Lenkei S, Raposo G, et al. The secondary antibodies used in this
study are goat Cy3-labeled anti-rabbit-IgG, goat Cy3-labeled anti-mouse-IgG, goat anti-mouse-Alexa
647, goat anti-rabbit-Alexa 647 (Molecular Probes), goat horseradish peroxidase (HRP)-conjugated
anti-rabbit-IgG and goat HRP-conjugated anti-mouse-IgG antibodies (Jackson ImmunoResearch
Laboratories). Winzler C, Rovere P, Rescigno M, Granucci F, Penna G, Adorini L, et al. Nuclei were
stained with DAPI and chlamydial inclusions were detected with a rabbit anti-CT529 antibody and a
secondary anti-rabbit Cy3-labeled antibody.