SPD 20 A

228-90095F
UV-VIS DETECTOR
FOR
SHIMADZU HIGH PERFORMANCE
LIQUID CHROMATOGRAPH
SPD-20A/20AV
SPD-20A UFLC
SERVICE MANUAL
CAUTION !
 This service manual has been edited for those that have received service related
training.
 Those that have not been trained, should not use this manual.
SHIMADZU
Analytical & Measuring Instruments Division
228-90095F
CONTENTS
Chapter 1 Introduction........................................................................................................................6
1.1 Introduction...........................................................................................................................................................................6
Chapter 2 Specifications......................................................................................................................7
Chapter 3 Measurement systems....................................................................................................7

3.1 Optical system......................................................................................................................................................................7
3.1.1 SPD-20AV Light path schematic............................................................................................................................9
3.1.2 SPD-20A Light path schematic............................................................................................................................10
3.2 Electrical system...............................................................................................................................................................11
3.2.1 Signal processing circuits.........................................................................................................................................11
3.2.2 Lamp power supply circuits...................................................................................................................................12
Chapter 4 Parts configuration and function...............................................................................13

4.1 Front side of instrument................................................................................................................................................13
4.2 Operation controls............................................................................................................................................................13
4.3 Inside the front cover, and its top and left sides....................................................................................................13
4.4 Right and bottom sides..................................................................................................................................................13
4.5 Back side..............................................................................................................................................................................13
4.6 Inside the instrument.....................................................................................................................................................13
4.7 Inside the optical unit.....................................................................................................................................................14
4.7.1 Illustration of optical unit interior.........................................................................................................................14
4.7.2 List of primary internal parts for optical unit..................................................................................................14
4.8 Temperature-controlled flow cell drawing and component parts list..........................................................15
4.8.1 Assembly drawing for temperature-controlled flow cell..............................................................................15
4.8.2 Component parts list for temperature-controlled flow cell assembly.....................................................16
Chapter 5 Other functions...............................................................................................................17

5.1 List of auxiliary functions..............................................................................................................................................17
5.1.1 Parameter group.........................................................................................................................................................17
5.2 List of VP functions..........................................................................................................................................................18
5.3 List of factory functions..................................................................................................................................................20
Chapter 6 Maintenance and adjustments..................................................................................22

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6.1 Cleaning the temperature-controlled flow cell......................................................................................................22

6.1.1 Simplified cleaning of temperature-controlled cell.........................................................................................22
6.1.2 Disassembled cleaning of flow cell........................................................................................................................22
6.1.3 Assembling the flow cell...........................................................................................................................................22
6.1.4 Cleaning the quartz window..................................................................................................................................22
6.3 Adjusting the optical system........................................................................................................................................24
6.3.1 Adjustment overview................................................................................................................................................24
6.3.2 Preparations.................................................................................................................................................................24
6.3.3 Optical adjustment jigs (targets)...........................................................................................................................25
6.3.4 Adjusting M1................................................................................................................................................................26
6.3.5 Adjusting slit position................................................................................................................................................27
6.3.6 Adjusting W lamp stopper screw.........................................................................................................................28
6.3.7 Adjusting M2................................................................................................................................................................29
6.3.8 Adjusting the grating................................................................................................................................................30
6.3.9 Adjusting M3................................................................................................................................................................31
6.3.10 Fine adjustment of mirror M1.........................................................................................................................32
6.3.11 Checking the filter position................................................................................................................................32
6.3.12 Adjusting the Hg lamp........................................................................................................................................34
Chapter 7 Additional explanations...............................................................................................36

7.1 Differences from previous models..............................................................................................................................36
7.1.1 WAVE ADJ / WAVE CHECK / WAVE CALIB functions.......................................................................36
7.1.2 Clearing lamp usage times.....................................................................................................................................36
7.1.3 Changing the gain of operation amplifier.........................................................................................................37
7.2 Additional VP functions.................................................................................................................................................40
7.2.1 Serial numbers............................................................................................................................................................40
7.2.2 Usage time....................................................................................................................................................................40
7.2.3 Log records....................................................................................................................................................................40
7.2.4 Date and time..............................................................................................................................................................40
7.2.5 Password........................................................................................................................................................................41
7.2.6 Absorbance compensation factor (ABS COMP).............................................................................................41
7.2.7 Automatic leak sensor correction (LEAK CALIB)........................................................................................41
7.2.8 Leak sensor threshold level (LEAK THR)........................................................................................................41
7.2.9 Compatibility of D2 lamps with previous models..........................................................................................42
7.2.10 Voltage at data recorder output terminal....................................................................................................42
7.2.11 How to update the ROM version....................................................................................................................43
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7.2.12 Changing the power supply input voltage..................................................................................................44

7.2.13 The cautions at the time of linearity compensation [LINEAR CALIB] operation.................................45
Chapter 8 Troubleshooting..............................................................................................................46
8.1 Using symptoms to identify the problem................................................................................................................46
8.2 Using the error display to identify the problem....................................................................................................46
8.3 Problems with electrical circuits and points to check.........................................................................................47
8.3.1 SPD20-MAIN circuit board....................................................................................................................................47
8.3.2 SPD20-PWR(POWER) circuit board.................................................................................................................49
Chapter 9 Electrical circuits............................................................................................................50

9.1 PCB diagram, SPD-20 cables.....................................................................................................................................50
9.2 Circuit diagram, LC20-KEY.......................................................................................................................................51
9.3 Circuit diagram, SPD20-MAIN.................................................................................................................................52
9.3.1 Circuit diagram ,SPD20-MAIN 1/8....................................................................................................................52
9.3.2 Circuit diagram, SPD20-MAIN 2/8....................................................................................................................53
9.4 Circuit diagram, SPD- POWER................................................................................................................................60
9.4.1 Circuit diagram ,SPD20-PWR 1/5.......................................................................................................................60
9.4.2 Circuit diagram, SPD- POWER 2/5....................................................................................................................61
9.5 Circuit diagram, PB-1 ASSY.......................................................................................................................................65
Chapter 10 Parts lists..........................................................................................................................66

10.1 Consumable parts list.....................................................................................................................................................66
10.2 Optical repair parts list..................................................................................................................................................66
10.3 Temperature-controlled flow cell and plumbing parts list...............................................................................66
10.4 Electrical parts list............................................................................................................................................................67
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Chapter 11 Optional parts.................................................................................................................68

11.1 Parts list and diagrams for optional parts..............................................................................................................68
11.1.1 Temperature-controlled semi-micro cell (228-45605-94).............................................................................68
11.1.2 Inert cell (without temperature control) (228-33338-91)........................................................................68
11.1.3 Preparative cell (variable capacity, without temperature control).....................................................69
11.2 Parts list and diagram for solvent recycle valve...................................................................................................70
【Amendment history】
D “5.3 List of factory functions SIGNAL MODE” was corrected.
“4.7.2 List of primary internal parts for optical unit List of primary internal
parts for optical unit” was corrected.
“9.2 Optical repair parts list”was corrected.
E Revisions to “4.8 Temperature-controlled flow cell assembly drawing and

component parts list”, “9 Parts List”, and “10 Optional parts”
F “6.3.10 Fine adjustment of mirror M1” was added to optical system adjustment
process, “6.3.3 Optical adjustment jigs (targets)” was added, the paper target copy was
discontinued, and “7.1.3 Changing the gain of operation amplifier” was added as
additional explanations. 7.2.13 Add Notes of Clause of Linearity Calibration.
6.3.12 Add the Method of Lighting of Hg Lamp by Clause of Adjustment of Hg Lamp.
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Chapter 1 Introduction
1.1 Introduction
This service manual applies to SPD-20A, SPD-20AV and SPD-20A UFLC
detectors. The difference between the construction of 20A and 20AV models is
the positioning of the tungsten (W) lamp, the W lamp power supply, and the
mercury (Hg) lamp used for wavelength calibration. The difference between the
-20A and -20A UFLC is the specification of the flow cell (-20A: standard cell,
-20A UFLC: semi-micro cell). The figures in this manual illustrate model 20AV,
but the explanatory text applies to both and having the instrument available to
look at should prevent any confusion. However, please note that parts with the
same name for all models may be differentiated using different part numbers.
This manual is intended to explain the maintenance of 20A (including -20A

UFLC) and 20AV model detectors to service personnel of the instruments. It
primarily describes general troubleshooting procedures for problems that could
occur during operation, in addition to maintenance and inspection procedures
that must be performed on a routine basis. The function of certain parts and
mechanisms are also explained for reference. When performing any work on the
detectors, use this manual in combination with the instruction manual for the
instrument. Please carefully observe the precautions for installation and
operation, and make sure the instrument is maintained appropriately and
effectively before using it.
CAUTION:
• This service manual includes only the table of contents entries and no
corresponding text for subjects addressed in the instruction manual. Please
use this manual in combination with the instruction manual.
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Chapter 2 Specifications
Please read a see instruction manual.
Chapter 3 Measurement systems

3.1 Optical system
Figure 3.1 illustrates the instrument’s optical system. It is equipped with a D2
lamp and W lamp (model 20AV) for the light source. The angle of mirror M1 is
used to choose between the two light sources. The angle of mirror M1 is switched
by the “LAMP” setting under the function key (operation controls). When the
mirror M1 position is switched, the lamp switching photomicrosensor detects the
angle of mirror M1, so only the selected light source stays on and the other lamp
turns off. In addition, if “LAMP” is set to “3”, then it is possible to select both
lamps. Model 20AV uses the D2 lamp for the UV region (190 -- 370 nm) and the
W lamp for the visible region (371 －900 nm). Model 20A uses only the D2 lamp
(190－700 nm).
When the angle of mirror M1 is in the position shown, the light from the D2 lamp
is reflected and focused through the quartz window and enters the slit. When the
angle position of mirror M1 is switched, the light from the W lamp passes
through a stray light cut-filter (short wavelengths) and heat-absorbing filter, then
in the same manner is reflected and focused by mirror M1 and passes through the
quartz window and enters the slit. A filter assembly (high order cut-filter or
blocking filter) is inserted in front of the slit, based on the wavelength setting.
The blocking filter is inserted to detect zero order light. The high order cut-filter
is inserted in front of the slit to filter higher order spectra in the specified
wavelength region corresponding to the wavelength setting. The filter is inserted
in front of the slit at the 371－700 nm range for model 20A and 701 nm -- 900 nm
for model 20AV. After the light beam passes through the slit, it is reflected and
focused by mirror M2 and hits a holographic grating of 1600 grooves per
millimeter, where it is separated into its spectrum. The light separated by the
grating emerges as parallel light beams that are reflected by mirror M3 and are
wide enough to straddle and enter both of the sample and reference cells. The
spectrum is generated in an orientation perpendicular to the line bisecting both
cells, so both cells receive identical light wavelengths. After passing through each
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cell, the respective light enters a silicon photodiodes (D1 and D2) where it is
converted to an electrical signal that is proportional to the light intensity.
The light entering the cells, or in other words, the wavelength being measured
during analysis, is selected by changing the angle of the grating. A stepping
motor is used to move the grating via a pulley and belt. When the instrument
power switch is turned on, mirror M1 moves to the angle position for the W lamp,
then again to the D2 lamp position. The lamp switching photomicrosensor detects
the position and the D2 lamp will turn on. When the mirror M1 position is
switched, the home position for the filter assembly is simultaneously detected.
Once the lamp goes on, the grating stepping motor first searches for the home
position of the wavelength setting, then verifies the wavelength using the D2
lamp and Hg lamp emission lines. This instrument measures the amount of light
passing through the cell as the wavelength is varied and automatically identifies
the wavelengths corresponding to the emission line spectrum peaks. If these
wavelengths are within one nanometer of the emission line wavelengths, then
“CHECK GOOD” will appear on the display. Then the grating rotates according
to the wavelength setting and the lamp position switches also. The sample cell
has a flow-through type structure. The reference cell is simply a hole that lets
light pass through. The Mercury lamp is used to check or calibrate wavelengths.
The characteristic emission line of Hg lamps at 253.7 nm is used as a reference
for checking accuracy in the ultraviolet region.
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3.1.1 SPD-20AV Light path schematic
SPD-20AV Light path schematic
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3.1.2 SPD-20A Light path schematic
SPD-20A Light path schematic
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3.2 Electrical system

The electrical system can be separated into four major sections, the signal
processing circuits, the light source power supply circuits, the drive circuits
(grating, filters and mirrors), and key board (operator controls).
3.2.1 Signal processing circuits
After passing through the measurement and reference cells, the respective light
beams (intensity) IM and IR enter silicon photodiodes D2 and D1. These
photodiodes generate an electrical current that is proportional to the quantity of
light. The currents K2IM and K1IR generated from D2 and D1 each flow
respectively through high resistances R2 and R1 and are converted to voltage by
A2 and A1. The respective outputs from A1 and A2 are converted to digital
signals by AD converters M22 and M23 and those values are converted by
logarithmic transformation in the CPU. The CPU processes the absorbance range,
zero adjustment and signal response from values entered via the operator
controls. The logarithmically transformed output voltages are reconverted to
analog voltage using a DA converters (M30 and M31) and a portion of that
voltage is sent via resistors to the AUX terminal as integrator output. Similarly,
resistors are also used to output 10 mV signals as data recorder output.
(Measurementside)
Schematic of signal processing circuits
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3.2.2 Lamp power supply circuits

The deuterium lamp (D2 lamp) is controlled via a fixed current circuit to provide
a stable light source that is not affected by power fluctuations. In addition, this
circuit has a fixed discharge current for automatically turning on the lamp. A
high voltage is applied and the filament is heated until the lamp is illuminated. If
the lamp does not turn on after several attempts, an “ERR D2 LAMP” message is
displayed. If the lamp turns on normally, “D2” will appear in the lower left part of
the initial display screen. The tungsten lamp (W lamp) is turned on and
stabilized via a fixed voltage supply. The display indicates when the W lamp is on
as well. Unlike models SPD-10AVvp and SPD-10AV, model SPD-20AV allows
both the D2 and W lamps to be illuminated simultaneously. (As noted above, the
position of mirror M1 switches according to the wavelength setting, so only the
light from one of the lamps will enter the cells. Furthermore, the cooling fans for
the lamp housings will turn on after the lamps have been on for thirty minutes.)
Highvoltage
triggercircuit
Transformerpowersupply
D2lamp
Fixed current
D2 circuit
Fixed voltage
D2 heater circuit
Fixed voltage Wlamp

W circuit
Lamp illumination circuit schematic
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Chapter 4 Parts configuration and function

4.1 Front side of instrument
4.2 Operation controls

4.3 Inside the front cover, and its top and left sides
4.4 Right and bottom sides

4.5 Back side

4.6 Inside the instrument

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4.7 Inside the optical unit

4.7.1 Illustration of optical unit interior
Optical unit interior

4.7.2 List of primary internal parts for optical unit
List of primary internal parts for optical unit
No. Name P/N Remarks No. Name P/N Remarks
1 Grating ASSY 228-15257-95 11 Hg lamp ASSY 228-38214-96
2 M2 mirror ASSY 228-23014-91 12 Filter ASSY (VIS) 228-42066-91 For V only
3 M3 mirror ASSY 228-23041-93 13 W lamp 670-14602 For V only
Filter ASSY (UV) 228-42008-91 For A
4 Motor ASSY, VP 228-23027-92 14
FilterASSY (UV/VIS) 228-42008-92 For V
5 Slit ASSY 228-23021-91 15 PB-1 SPD20A ASSY 228-23691-95
Standard
temperature-cont
6 Window 228-34795 228-37440-94
olled flow cell
16 ASSY
Temperature-controll
For UFLC.
7 D2 lamp 228-34016-02 ed semi-micro flow 228-45605-94
cell ASSY
Silicon photocell
8 Stopper screw 228-15664-06 17 228-23016-91
ASSY
9 Stopper 228-25428
10 M1 Assy 228-23037-95
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4.8 Temperature-controlled flow cell drawing and component

parts list
4.8.1 Assembly drawing for temperature-controlled flow cell
(Standard: 228-37440-94, Semi-micro: 228-45605-94)
Reference: Procedure for tightening lens fixing screws (part 16) (factory method)
1) Verify that the lens surface fits completely inside the counterbore in the
housing. Do not use gaskets that are too warped. Doing so can raise the
lens and cause it to break during tightening.
2) Use a torque screwdriver to temporarily tighten screws to 0.4 Nm (4
kgf·cm).
3) Use a torque screwdriver to finish tightening screws to 0.8 Nm (8
kgf·cm).
4) Verify there is no lens breakage.
5) Retighten screws to 0.8 Nm (8 kgf·cm) eight hours later.
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4.8.2 Component parts list for temperature-controlled flow cell assembly

Ref. Stan Semi-
No. Part Name Part Number Remarks
dard micro
1 Semi micro cell housing 228-48646-01 1
1 Cell housing 228-41992-01 1
2 Cell holder 228-37442 1
2 Cell holder 228-41031 1
3 Cell plate 228-38465-02 1
3 Cell plate 228-41038 1
4 Inner cover, cell 228-37445 1
4 Inner cover, semi micro cell 228-37445-01 1
5 Outer cover, cell 228-37446 1 1
6 Cell fixing screw ASSY 228-38390-91 2 2
8 Inlet pipe ASSY (XP) 228-40347-91 1
8 Inlet pipe ASSY 1040 228-47522-94 1
9 Pipe, SUS316L D0.8 228-40348-02 1
9 Pipe ASSY, OUT L210 228-47524-92 1
10 Ferrule 0.8F 316L 228-40997-10 1 1
11 Male nut 0.8MN -M4 228-42605 1 1
12 Ferrule 0.8F 316L 228-40997-10 1 1
13 Male nut 0.8MN-M4 228-42605 1 1
14 Gasket 228-35097-01 2
14 Gasket 228-35097-05 2
15 Lens (R4, RD6) 228-14572 1 1
16 Lens fastener screw ASSY 228-40239-91 2 2
18 Optical Window 228-18058 1 1
19 Washer,SUS spring 2 M2.6 023-77026 2
20 Screw,SUS pan head M2.5X4 020-05121 2
20 Screw,SUS sems P2 M3X14 020-46131 2
21 Screw,SUS sems P4 M3X8 020-46635 1 2
22 Screw,SUS flat head M3X6 020-12104 1 1
23 Screw,SUS sems P4 M3X6 020-46634 3 3
1. Transistor TR2SD2639E
2. Thermostat Active: 60
24 Heater SPD-20 ASSY 228-40189-92 1 1 Reset: 40
3. Thermistor
R25=10kΩB25/85=4.128kΩ
25 Screw,PA pan head M3X8 020-03144 4 4
27 Male nut 0.8MN-PEEK 228-46363 1
29 Male nut 0.8MN-PEEK 228-46363 1
30 O-ring,1A P6 036-10204 2 2
34 Seat, thermally conductive 228-41049 1 1
36 Ground plate, cell 228-40503 1 1
37 TR bushing B-24 060-49746-16 1 1
50 Coupling 1.6-0.8C 316L 228-40998-10 1
51 Coupling 1.6-0.8C 316L 228-40998-10 1
52 Seal, Tape 228-50503
53 Seal, Sponge 228-50504
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Chapter 5 Other functions

5.1 List of auxiliary functions
This instrument includes the following auxiliary functions. Settings can be
changed in sequence by pressing the “Func” key for each function group.
5.1.1 Parameter group
No. Name Function Key(s)
1 LAMBDA Sets wavelength being measured Numeric keypad
2 RESPONSE Sets response speed Numeric keypad
3 LAMP Sets lamp on/off settings Numeric keypad
4 AUX RANGE Sets range of output to integrator Numeric keypad
5 RANGE Sets measurement range Numeric keypad
6 REC MODE Sets output mode to recorder Numeric keypad
7 BL OFS ITG Sets zero position of integrator Numeric keypad
8 BL OFS REC Sets zero position of recorder Numeric keypad
9 CELL TEMP Sets temperature of temperature-controlled flow cell Numeric keypad
10 EVENT Sets status of external event output terminal Numeric keypad
11 RT RNG Sets output range of ratio chromatogram Numeric keypad
12 RT THR Sets threshold value of ratio chromatogram Numeric keypad
13 SV LEVEL Sets threshold value for switching solvent recycle valve Numeric keypad
14 DELAY TIME Sets delay time for solvent recycle valve Numeric keypad
5.1.2 Control group

1 WAVE CHECK Checks wavelength precision Enter
2 W POWER Sets brightness of tungsten lamp. Numeric keypad
10.0V at W POWER =0, plus 0.2 V per additional 1
3 SCAN FILE Sets file numbers for storing scan data Numeric keypad
4 SCAN BGN Sets starting wavelength of scan Numeric keypad
5 SCAN END Sets ending wavelength of scan Numeric keypad
6 SCAN STEP Sets wavelength scan interval size Numeric keypad
7 PLOT SPD Sets speed of sending spectral data output to data recorder Numeric keypad
8 SPC PLOT Outputs spectral data to data recorder Enter
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5.1.3 System group

1 LOCAL Sets whether to control from system controller or from local Numeric keypad
unit
2 LINK ADRS Sets address when controlling from system controller Numeric keypad
3 KEY CLOSE Blocks input via keys Enter
4 BRIGHTNESS Adjusts contrast level of display Numeric keypad
5 EXT-S Used when the event terminal is used to start external Numeric keypad
devices, etc.
6 MONIT-TIME Displays elapsed time during time program execution Numeric keypad
5.1.4 Monitor group

1 SMPL EN Displays sample light output level display only
2 REF EN Displays reference light output level display only
3 D2 TIME Displays deuterium lamp total usage time display only
4 W TIME Displays tungsten lamp total usage time display only
5 CELL TEMP Displays temperature monitor for temperature-controlled flow display only
cell
5.2 List of VP functions

VP functions are listed below. Settings can be changed in sequence by pressing
the “Func” key for each function group.
5.2.1 Product information group

1 SERIAL NUMBER Displays serial number display only
2 Software ID Displays instrument name and ROM version display only
5.2.2 Maintenance information group
1 TOTAL OP TIME Displays total operating time of instrument display only
2 D2 LAMP USED Displays D2 (deuterium) lamp usage time and estimated display only
replacement time
3 W LAMP USED Displays W (tungsten) lamp usage time and estimated display only
replacement time
4 PART Used to enter part replacement record. Numeric keypad
REPLACEMENT
5 MAINTENANCE Displays maintenance log display only
LOG
6 OPERATION LOG Displays operation log display only
7 ERROR LOG Displays error log display only
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5.2.3 Validation Support group

1 DATE Displays or enters date tenkey
2 TIME Displays or enters current time tenkey
3 AUTO CHECK Checks memory, wavelength accuracy and light intensity Enter
4 LEAK SENSOR Checks leak sensor Enter
TEST
5.2.4 Calibration Support group

1 INPUT Used to enter password. tenkey
PASSWORD
2 WAVE CALIB Calibrates wavelength Enter
3 D2 TIME Used to enter D2 (deuterium) lamp replacement schedule. tenkey
4 D2 ENERGY Used to enter energy values for D2 (deuterium) lamp replacement tenkey
schedule.
5 W TIME Used to enter W (tungsten) lamp replacement schedule. tenkey
6 W ENERGY Used to enter Energy values for W (tungsten) lamp replacement tenkey
schedule.
7 ABS CALIB Correction applied to absorbance data Enter
8 ABS COMP Used to enter correction factor for absorbance. tenkey
9 LINEAR CALIB Linearity calibration 1:BG 2:4AU 3:deb* Enter
* It is displayed at the time of FACTORY mode.
Linearity compensation will become effective if [3] is inputted even
if it does not return on a power supply switch.
4 (not displayed) :An input of [4] makes compensation of linearity
initial value.If a power supply switch is returned on, linearity
compensation will become effective as before.
3 and 4 are the commands for debugging.Fundamentally, please
do not use 3 and 4.
10 LEAK CALIB Automatic correction applied to leak sensor Enter
11 LEAK THR Used to enter leak sensor threshold level. tenkey
12 RNG DISP MODE Sets full scale display mode for absorbance tenkey
13 OP MODE Sets operating mode tenkey
14 INITIALIZE Initializes parameters Enter
PARAM
15 CHANGE Changes password Enter
PASSWORD
16 CBM Sets CBM (SCL) parameters Enter to Select
PARAMETER
17 SERIAL NUMBER The serial number of CBM can be displayed. display only
It can set up on a back screen.
18 SOFTWARE ID Displays CBM system type and ROM version display only
19 INTERFACE Sets CBM connection interface tenkey
20 ETHERNET Sets CBM Ethernet transmission rate tenkey
SPEED
21 DHCP SERVER Can be set only when parameters sent using HDLC are enabled tenkey
22 IP ADRESS Can be set when parameters sent using HDLC are enabled and tenkey
DHCP server is not used
23 SUBNET MASK (same as above) tenkey
24 DEFAULT (same as above) tenkey
GATEWAY
25 TRS MODE RS-232C transfer mode tenkey
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5.3 List of factory functions

If this instrument is started up in “special mode” (power switch is turned on while
pressing the “Func” key), then the factory function group is added to the available
auxiliary functions. Factory functions are listed below.
* Functions indicated with an asterisk (*) will only display at the factory.
LAMBDA 1
1 See parameter group tenkey
LAMBDA 2
2 RESPONSE See parameter group tenkey
Sets lamp On/Off status 3 (henceforth) is not displayed.
0: all OFF 1: D2 ON 2: W ON 3: D2 + W ON
*3 LAMP
4: Hg ON 5: D2 + Hg ON 6: W + Hg ON 7: D2 + W + Hg tenkey
ON
4 AUX RANGE See parameter group tenkey
5 RANGE See parameter group tenkey
SMPL EN
6 See monitor group display only
REF EN
7 W POWER See control group tenkey
Moves GM step position (absolute) from HP sensor position by one
tenkey
*8 ABS GM STEP step
0: DEC 1: INC
Starts or cancels auto-zeroing function
*9 Z WAVE tenkey
0: Start 1: Disable 3: Stops GM excitation
Overrides FM position
0: No filter in optical path
*10 FILTER POSI tenkey
4: Insert second-order light cut filter into optical path
7: Insert zero-order light cut filter into optical path
Overrides LM position
*11 LAMP POSI tenkey
0: Automatic insert 1: D2 position 2: W position
Sets signal mode
0: With S/R log conversion 1: With S log conversion
2: With R log conversion
*12 SIGNAL MODE 4: Without S/R log conversion 5: Without S log conversion tenkey
6: Without R log conversion
7: Simultaneously output S/R (without log) from ITG and REC
(For development)
13 REC MODE See parameter group tenkey
*14 WAVE ADJ Calibrates wavelength Enter
15 WAVE CHECK Checks wavelength precision Enter
16 SV LEVEL See parameter group tenkey
17 EVENT See parameter group tenkey
18 LINK ADRS See system group tenkey
19 LOCAL See system group tenkey
Displays D2 lamp anode current value (mA)
*20 LAMP CUR display only
Normal value: 300 ±30
Displays D2 filament current value (mA)
*21 D2 FIL CUR display only
*22 D2 TRIG CT Displays D2 lamp trigger count display only
Displays W filament current value (mA)
*23 W FIL CUR display only
24 CELL TEMP See monitor functions group display only
Displays aluminum block temperature
*25 RADIATOR T Normal value: (ambient + 30C) x 10, where “ERR OVER HEAT” display only
displayed at 710C or above
Displays leak sensor liquid temperature
*26 LEAK SENSE display only
Normal value: (ambient + 30C) x 10
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Displays leak sensor room temperature

*27 LEAK ROOM display only
Normal value: (ambient + 5C) x 10
Corrects temp-controlled flow cell at 50C tenkey
*28 CELL T COR
Temp difference for 50C measurement is entered (Step 0.1C)
Sets temp-controlled flow cell PID parameter
*29 PID P tenkey
(Do not change this control parameter)
*30 PID I tenkey
*31 PID D tenkey
Allows the following settings at the bit level. Sets sum total of each
value. Normally setting is “2”.
2: Allows input of 0 for RESP
4: Allows 180 nm setting
8: Stops “OVER” display and continuously displays
absorbance
258: Skips wavelength check for 254 nm (Hg)
2048: Displays wavelength check results instead of SE/RE and
sends results to SCL
*32 CUSTOMIZE 4096: Measures data processing time and checks REC/ITG tenkey
output (for development use)
8192: Increases digital data monotonically (for development
use)
16384: Checks digital triangular wave (1 min up to 1 mAU and 1
min down to 0 mAU) and integrity of HDLC frames (for
development use)
32768: Reverses “FUNC” logic (Normally startup -> special mode
-> If "FUNC" is pushed and a power supply switch is
turned on -> normal mode)
*33 CLEAR ALL Initializes logs and backup memory Enter
Used to enter instrument type.
*34 PRODUCT TYPE tenkey
1: 20A 2: 20AV
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Chapter 6 Maintenance and adjustments

6.1 Cleaning the temperature-controlled flow cell
6.1.1 Simplified cleaning of temperature-controlled cell

6.1.2 Disassembled cleaning of flow cell

6.1.3 Assembling the flow cell

6.1.4 Cleaning the quartz window

(1) With the instrument power supply turned off, disconnect the D2 lamp
connector and remove the D2 lamp. (Perform this step after the D2 lamp
has cooled sufficiently.)
(2) Remove the plate attached inside the lamp housing (two screws). A spring
is enclosed behind the plate, so loosen both screws evenly.
Screws
(3) A quartz window is located behind the spring. Pull the spring out slowly
and remove the window.
(4) Wipe any dirt from the window using a Kimwipe, etc., and reassemble the
parts.
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6.3 Adjusting the optical system

6.3.1 Adjustment overview
Adjustment of models SPD-20A (including -20A UFLC.) /SPD-20AV involves
adjusting the D2 lamp, mirror M1, W lamp (model SPD-20AV only), Hg lamp,
filter (model SPD-20AV only), slit, mirror M2, grating, mirror M3 and cell, as
shown below. The procedure for adjusting each component is explained below.
6.3.2 Preparations
Before adjusting any component, please prepare the following.
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6.3.3 Optical adjustment jigs (targets)

Below is the list of optical adjustment jigs (targets). They are targets with a
reinforcing material (plastic frame). Use these jigs for optical adjustment.
List of Optical Adjustment Jigs
No. Jig P/N Comment
1 Jig for M1 221-94566-91 Delivered product, stocked
2 Jig for fine adjustment of M1 221-94566-92 New product, newly stocked
3 Jig for GR 221-94572-91 Delivered product, stocked
4 Jig for GR (large)* 221-94572-92 New product, newly stocked
5 Dummy cell with target 228-32686-92 Delivered product, custom product
6 Jig for M2 228-34949-91 Delivered product, stocked
7 Jig for M3, SPD-20A 228-46069-91 Delivered product, newly stocked
* Some gratings have a larger width due to a variation during processing (cutting). Use
the jig for gratings with a large width. In most cases, 221-94572-91 for regular size can
be used.
(1) Make a full-size copy of the next page (6.3.3 Optical adjustment jigs
(targets)), cut the targets out with a box cutter, etc., assemble them as
illustrated, and use them as adjustment targets. The M3 target is also
used for the new type M3 mirrors in LC-2010 systems. Also, except for the
M3 target, the other targets can also be used for SPD-10vp series
instruments.
(2) Open the lamp housing and spectroscope room by loosening the screws on
the top covers (4 screws on lamp housing and 5 screws on spectroscope
room).
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6.3.4 Adjusting M1
> In order to make it factory mode
(1) Turn the power on while pressing the “func” key.
> push func →Factory→and Set λ＝0nm
(2) Turn the D2 lamp ON.
(3) Attach the M1 and M2 jigs.
(4) (For model SPD-20AV, use a clip or other means to secure the lever pin
attached to the M1 assembly and the stopper pin.
(5) With the slit assembly removed, adjust the M1 assembly so the light
image hits the center of the target mark on the M2 jig.
Lateral adjustments to image on M2 jig (Paper Target):
Loosen screws “A” on the M1 assembly and rotate the assembly.
Vertical adjustments to image on M2 jig:
Adjust the angle of elevation using the screws “C”. Do not loosen set
screws “B” and the nuts that secure them. After making adjustments,
tighten screws “A”.
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6.3.5 Adjusting slit position

Attach the slit assembly and adjust it in the X-direction so the image center
(the brightest portion) is projected on the slit, then secure the slit assembly.
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6.3.6 Adjusting W lamp stopper screw

(1) Remove the clips used to secure the M1 assembly.
(2) Turn the W lamp on.
(3) Loosen the nut on the M1 assembly, adjust the stopper screw so the image
projected on the slit assembly is horizontally centered and retighten the
nut.
(4) After making adjustments, switch the M1 assembly so it faces the D2
position and turn on the D2 lamp.
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6.3.7 Adjusting M2
(1) Remove the M2 jig and attach the M2 assembly.
Attach the M3 jig to assembly M3. Press the function key and set “Z
WAVE”, in the factory group, to 3.
(Wavelength still = 0nm)
(2) Roughly adjust the rotation of M2 and GR so the 0-order light from M2
passes via GR and is laterally centered on M3 (within Φ10mm circle).
Next, attach the GR jig and adjust the M2 assembly rotation so the light
image is laterally centered on the GR target mark. Roughly adjust the
vertical angle of M2 at this point. (Rotation: Adjust by loosening screws
“A” and rotating. Elevation: Adjust by loosening nut and turning set screw
“B”.)
(3) After making adjustments, tighten screws “A” and remove the GR jig, but
don’t tighten the nut at this point.
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6.3.8 Adjusting the grating

> It continues. Z-wave =3.λ=0nm
(1) Rotate the grating in direction “A” shown in the figure and look at the
spectrum projected on M3. Loosen the grating lock screw and rotate the
grating in direction “B” in the figure. Secure the grating in location when
the spectrum becomes horizontal.
(2) Readjust the vertical angle of assembly M2 so the image is vertically
centered on the M3 jig and tighten the nut.
(3) Adjust the grating by rotating it in direction “A” shown above until the
0-order light image reaches a lateral position on the M3 jig that is 1 mm
(±0.3 mm) to the left of center.
(4) Remove the M3 jig.
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6.3.9 Adjusting M3
(1) Attach the cell jig.
(2) Adjust the rotation and elevation angle of the M3 assembly so the 0-order
light image hits the center of the target on the cell jig.
(3) Remove the M1 jig, attach a dummy cell, and double check that the long
narrow 0-order light image is over the cell hole.
Caution:Be careful not to touch the mirror or grating surfaces with your hands.
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6.3.10 Fine adjustment of mirror M1

(1) Light up the D2 lamp.
(2) Direct the Mirror M1 ASSY to the D2 lamp.
Jig for fine
(3) Attach the jig for fine adjustment of M1 on adjustment of M1
mirror M1.
(4) Set the pin of the lever (rotating table)
where the mirror M1 ASSY is mounted and
the stopper pin on the lamp housing using a
clip or something similar.
M1 ASSY
(5) Adjust the direction of the mirror M1 ASSY
so that two optical images on the slit (figure
below) go into the slit. (Unfasten screw A on
the mirror M1 ASSY and rotate the mirror
M1 ASSY.)
(6) After adjustment is completed, tighten screw A to fix it and detach the jig
for fine adjustment of M1 and clip.
D2 lamp
Slit
スリット
Slit
Rotation
Pin of the lever
Optical
光の像 Stopper pin
images
M1 ASSY Screw A
Clip
6.3.11 Checking the filter position

(1) Turn on the power while pressing the “Func” key.
(2) Use the “FILTER POSI” function from the factory functions group to
display the filter position and set it to “0”. Check that the filter semi
assembly is not blocking any part of the slit.
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(3) Set ”FILTER POSI” to “4” and check that the second-order light cut-filter
covers the entire front surface of the slit.
(4) Set ”FILTER POSI” to “7” and check that the deep green filter covers the
entire front surface of the slit.
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6.3.12 Adjusting the Hg lamp

(Model SPD-20A)
(1) 4 is inputted into LAMP in FACTORY mode, and only Hg lamp is made to
turn on.
(2) 2 is inputted into LAMP POSI and a lamp change mirror is changed to the
direction of Hg (V is W lamp) lamp.
(3) After adjust the M1, slit, M2, GR and M3 assemblies.
(4) Loosen the nut, turn stopper screw “A” and rotate M1 until the Hg lamp
image is centered over the monochromator slit. Once adjusted, secure the
stopper screw in place by tightening the nut.
Brightest Image
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(Model SPD-20AV)
The Hg lamp will project multiple images onto the slit from multiple
reflections in the VIS filter, as shown below. Adjust the Hg lamp by moving
it in the direction of the arrow shown below, until the brightest Hg lamp
image hits the center of the monochromator.
Brightest Image
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Chapter 7 Additional explanations

This service manual refers to special mode functions (power is turned on while
pressing the “Func” key), but this mode is reserved for Shimadzu service
engineers. To avoid confusion, do not reveal this mode to customers.
7.1 Differences from previous models

7.1.1 WAVE ADJ / WAVE CHECK / WAVE CALIB functions
The WAVE ADJ function was moved from being an AUX function to a
special mode function on SPD-10Avp and -20A models. In its place, the
WAVE CALIB function was added to the VP group. This is to prevent
operators from indiscriminately calibrating wavelengths.
The WAVE CALIB function is basically the same as combining the WAVE
ADJ and WAVE CHECK functions, but features such as leaving a log
record of calibration results have been added. Therefore, for SPD-10Avp
and -20A models please use WAVE CALIB instead of WAVE ADJ.
SPD-10A SPD-10Avp/ -20A Description

Yes Models 10A, 10Avp and 20A are the
WAVE ADJ Yes (special mode only) same
WAVE CHECK Yes Yes Models 10A, 10Avp and 20A are the
same
WAVE CALIB No Yes Almost same as WAVE ADJ + WAVE
CHECK
7.1.2 Clearing lamp usage times

Clearing D2 and W lamp usage times on models SPD-10Avp and -20A has
changed from being an AUX function to being a VP function. This is so
maintenance information can be collectively displayed and operated on in
one location. When “0” is entered for “D2 LAMP USED” or “W LAMP
USED”, the lamp usage time is cleared and lamp replacement information
is simultaneously recorded in the “MAINTENANCE LOG”. As before,
usage times can be displayed using AUX functions.
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7.1.3 Changing the gain of operation amplifier

After the mirror M1 is fine-adjusted as described in Amendment F in this
Service Manual, optical energy may become saturated. Check the energy
after adjustment. If the energy is over the standard level, lower the gain
of the operational amplifier by the procedure described below.
7.1.3.1 Checking the light intensity

After the optical system is adjusted using the added jig (for fine adjustment of M1),
the light intensity may increase by several percent. This may cause saturation of
light intensity due to a variation of the lamp or optical element. When no measure
has been taken for the pre-amplifier (PB-1 ASSY), attach a new D2 lamp and check
the light intensity by the procedure described below. If the light intensity is over the
standard level*1, lower the gain resistance of the pre-amplifier by 7 % using a jumper
wire and solder. This modification is unnecessary for instruments where a
pre-amplifier for micro cells*2 is attached.
＊ 1: The light intensity standard (upper limit) differs according to the type of the
MAIN board (PCB, SPD20-MAIN ASSY); new or former. The new and former
boards can be distinguished by the BOARD identification no.*3 serigraphed on
the MAIN board.
＊ 2: The gain of the pre-amplifier on the RE side is maintained at a low level on
instruments where a micro cell is attached. Thus, the light intensity does not
saturate even after the optical system is adjusted using the added jig (jig for fine
adjustment of M1), leaving enough allowance. Therefore, modification is
unnecessary.
＊ 3: How to distinguish new and former MAIN boards
They can be distinguished by the BOARD identification no. serigraphed on the
MAIN board.
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 Upper limit of the light intensity when the identification no. is “228-42031B”
Cell SE RE
When using a standard cell 2200 mV 2200 mV If the value is over the upper
When using a semi-micro 1400 mV 2200 mV limit, the operational
cell amplifier needs to be
modified.
When using a micro cell Modification is unnecessary.
 Upper limit of the light intensity when the identification no. is “228-42031C”
or“228-53021”
Cell SE RE
When using a standard cell 3100 mV 3100 mV If the value is over the upper
When using a semi-micro 2000 mV 3100 mV limit, the operational
cell amplifier needs to be
modified.
When using a micro cell Modification is unnecessary.
7.1.3.2 Procedure to check the light intensity

(1) Attach a new D2 lamp and light it up.
(2) Fill the flow cell with deionized water
(3) Set the wavelength to 220 nm.
(4) Check light intensities SE/RE. If the value is over the standard value, lower
the gain of the pre-amplifier (PB-1 ASSY) by the procedure described below.
Modification is unnecessary if the light intensity is under the upper limit.
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7.1.3.3 Changing the gain of pre-amplifier (PB-1 ASSY)

If the light intensity is over the standard level after the optical system is adjusted,
lower the gain of the pre-amplifier by 7 % by the procedure described below.
＊ The lamp and optical elements (e.g. mirror, grating) of instruments where the
pre-amplifier has been modified may have to be replaced in several months to
several years depending on use frequency. In this case, the light intensity may
exceed the standard value due to a variation of the lamp or optical element
attached. However, no additional modification is necessary for instruments for
which the pre-amplifier has been modified once because there is enough
allowance in light intensity saturation voltage.
＊ If wax contained in soldering material remains on the surface of the board after
modification, it may cause poor insulation and eventually noise. If the board is
soiled, wipe it off with Kimwipe with a small amount of ethanol or IPA.
＊ Take out the PB-1 ASSY from the optical unit and modify the soldered face of the
PB-4 with an alignment of gain resistors mounted on the PB-1 ASSY using a
jumper wire* and solder as shown in the figure below.
* Recommended lead Conductor

Configuration Outer diameter
wire cross-section area
AWG #30 0.05 mm2 7 pcs./0.102 mm 0.30 mm
[PB-1 ASSY Modification Drawing]
Solder two positions on the soldered

face of PB-4 mounted on the PB-1
ASSY using an AWG30-level lead
wire and short-circuit it. (Left end of
R6:1 M and R7:2 M, both ends of
R23:1 M)
PB-1ASSY
PB-4 Enlarged soldered

face of PB-4
Optical unit
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7.2 Additional VP functions

7.2.1 Serial numbers
Instrument serial numbers have not been assigned when shipped from the
factory (numbers are set to “00000”). Please set the correct serial number
when installing the system.
(How to set the serial number)
① Startup system in special mode
② Go to the serial number function in the VP group
③ Enter the serial number corresponding to the label included with the
main unit.
(The beginning digits have already been set.)
CAUTION
Serial numbers are 14-charater codes beginning with the letter “L”.
Correctly enter the first 12 characters, but the last two characters (an
alphanumeric code indicating the manufacturing plant) do not need to be
entered.
Example: L21004101234J9 = L2 100 41 01234 J9
L2: LC model type 100: model # 41: Production time
01234: sequential # J9: factory
7.2.2 Usage time

Clearing the D2 and W lamp usage times was explained above, but if the
usage time must be changed for whatever reason (replacing a circuit
board or loss of memory, etc.) startup the system in special mode. The
time settings for ”TOTAL OP TIME”, ”D2 LAMP USED”, and ”W LAMP
USED” can each be changed in the special mode. A lamp replacement
record will be recorded in the ”MAINTENANCE LOG” even when
changing the “D2 LAMP USED” or ”W LAMP USED” values.
7.2.3 Log records

None of the log records can be deleted.
7.2.4 Date and time
Each log records the date and time of each event, but model SPD-20A does
not have a backup clock, so the expression of date and time may differ
depending on the system status.
① When an SCL (CBM) controller is connected or when date and time
settings have been entered, dates for log entries will use the format
yy-mm-dd.
② When an SCL (CBM) controller is not connected or when date and
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time settings have not been entered, dates for log entries will use total
system usage time values, expressed in the form T000001 (total usage
time of one hour)
7.2.5 Password
Instrument calibration and hardware related settings are performed using
the calibration support group functions. Password security is provided to
prevent operators from changing these settings. Passwords are set to
“00000” when shipped from the factory, but customers (instrument
administrators) can change it freely. In the event a customer forgets a
changed password, perform the following.
① Startup the instrument in special mode
② Enter “00000” for the password setting (the special mode allows either
the correct password or 00000)
③ Set a new password
7.2.6 Absorbance compensation factor (ABS COMP)

This is a correction factor used to compensate for differences in
absorbance resulting from variations in hardware between SPD
instruments (such as flow cell length). For example, if a coefficient of 0.9
is entered, absorbance will increase by about 11 percent (1/0.9 = factor of
1.111), but a setting of 1.000 should normally be used.
7.2.7 Automatic leak sensor correction (LEAK CALIB)

This feature automatically corrects for differences in sensitivity between
the measurement side leak sensor and reference side leak sensor. If the
leak sensors are left dry, ”LEAK CALIB” is displayed and the Enter key is
pressed, then the leak sensors will be calibrated automatically. Always
execute the ”LEAK CALIB” function after replacing a leak sensor.
7.2.8 Leak sensor threshold level (LEAK THR)

The threshold level is determined according to the following procedure.
(The “XXX” portion of “ActLv XXX/255” is read as the threshold value.)
① Read leak sensor output with no leakage. (A)
② Dribble water on the leak sensor and read its output. (B)
③ Add a value that is about 0.7 times the difference between reading A
and B to A, and set it as the threshold value.
A + 0.7× (B - A)
CAUTION
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① Always execute the automatic leak sensor correction (LEAK CALIB)

function before changing the leak sensor threshold level (LEAK THR).
② Check that the leak sensor tip is not touching anything plastic.
③ Keep the front panel closed during measurements.
7.2.9 Compatibility of D2 lamps with previous models
D2 lamps for SPD-10A and SPD-M10Avp models can be used in SPD-20A
systems. The intercompatibility of D2 lamps is shown below. In addition, D2
lamps for model SPD-20A are compatible with SPD-10Avp systems.
Instrument model
SPD-10A SPD-10Avp SPD-20A SPD-M10Avp Remarks
SPD-10A Operating life
Yes Yes* Yes* No
(670-14603) = 1000 hrs
SPD-10Avp,-20A
Yes Yes Yes No
(22834016-02)
SPD-M10Avp
Yes Yes Yes Yes
(228-34016)
* Note the guideline for initial replacement is 2000 hours.
7.2.10 Voltage at data recorder output terminal

The recorder output terminal voltage for model SPD-20A is 10 mV. If the
recorder input full scale is 1 mV, use one of the following methods to change
the full scale level.
① Set the AUX function RANGE setting to1/10.
② Reduce the output voltage by inserting a resistor between the SPD
system and recorder unit.
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7.2.11 How to update the ROM version

(Jigs required for update)
OPT-232C Adapter P/N 228-35480-91
② RS-232C Cable
③ Optical cable
④ Computer (with RS-232C connection capability)
(Procedure)
① Connect the instrument to be updated to the computer via the
OPT-232C adapter and start the computer.
② Start the SPD system in the version update mode (turn on the power
while pressing the VP key). The instrument display will show
“Monitor-20A Receive Ready“.
③ Open the version update application file “Fupdate.exe”.
④ Choose a COM port, select the ROM firmware (spdv xxx .rom: where
“xxx” indicates the version number), and begin updating by clicking
“Start”. The instrument display will show “Monitor-20A wait
Completion“.
⑤ Once the update is complete, the computer will display “ROM UPDATE
IS COMPLETED”.
⑥ The instrument display will not change even after the update has been
completed. Briefly turn off the power and restart the instrument.
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7.2.12 Changing the power supply input voltage

On SPD-20A and SPD-20AV models, the power supply input voltage is
switched by turning a rotary power supply input voltage switch to the
desired input voltage. However, always disconnect the power supply plug
before performing this procedure. Also, be sure to replace the fuse if the
power supply input voltage setting has been changed.
• 100-120V input Fuse 4AT, 250V P/N:072-02004-22

• 220-240V input Fuse 3.15AT, 250V P/N:072-02004-21
(1) Open the fuse holder with a flat head screwdriver.
(2) Turn the rotary power supply voltage switch to the desired supply
voltage.
(Alternatively, the rotary switch may be removed, set to the desired
power supply setting and reattached.)
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7.2.13 The cautions at the time of linearity compensation [LINEAR

CALIB] operation
If CE key is pushed suddenly and linearity compensation is canceled in the
middle of operation of linearity compensation, while the energy by the side of a
rewriting rate and a sample had also displayed 0mV on the unusual parameter,
the output according to energy will become impossible.
In such the state, since 253.7nm (Hg) and 656.1nm (D2) are undetectable,
[CHECK NO GOOD] is displayed.
Please perform linearity compensation correctly.
When sample side energy is set to 0mV, please take a measure in the following
procedure.
(1) [4] inputs [LINEAR CALIB] while on display, and make linearity
compensation into initial value.
(2) Perform wavelength proofreading.
(3) Perform linearity compensation again.
(4) Please once end re-start a power supply.
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Chapter 8 Troubleshooting
8.1 Using symptoms to identify the problem
8.2 Using the error display to identify the problem

No. Error Message Cause and remedial measure Error Type
1 ROM FAILURE ROM Sum error → replace PCB Fatal
2 RAM FAILURE RAM Matching check error→ replace PCB Fatal
Grating motor HP sensor error
3 ERR GR HOME POS Fatal
→ loose cable, defective sensor, defective PCB
Filter motor HP sensor error
4 ERR FIL HOME POS Fatal
HP sensor error for lamp switching
5 ERR LAMP HOME POS Fatal
EEPROM writing error
6 ERR EEPROM WRITE → defective PCB, defective device (guaranteed to Fatal
100,000 cycles)
Leakage detected
7 ERR LEAK DETECT Alarm
→ Leaking liquid, thermistor contact, defective PCB
Loss of EEPROM settings (sum error)
8 NOT PROTECTED → NMI writing error, power turned off during edit, Alarm
PCB defect, noise
656/254 wavelength differential greater than 1 nm.
9 CHECK NO GOOD → Cell attachment, bubble intrusion, inappropriate Alarm
sample, D2 or Hg lamp defect, GM threshold defect
D2 lamp anode current monitor defect
10 ERR OVER CURRENT Alarm
→ D2 lamp defect, PCB defect
D2 lamp will not go on even after retrying
11 ERR D2 LAMP Alarm
→D2 lamp defect, PCB defect
Overheated D2 lamp circuit radiator→ Open cover &
12 ERR OVER HEAT Alarm
fan, D2 lamp defect, PCB defect
Lamp does not light during ZERO & MARK
13 LAMP NO RIT Warning
→ Turn on the lamp.
D2 Lamp does not light in wavelength mode 2 during
14 SELECT D2 SINGLE WAVE CHECK Warning
→ D2 malfunction, change to wavelength mode 1
Wavelength setting defect in wavelength mode 2
15 SET ANOTHER λ Warning
→ Set without straddling 370/700.
Key operated during KEY CLOSE mode
16 KEY CLOSED Warning
→ Press Del+CE to cancel KEY CLOSE
Spectrum outputted without scanning
17 DATA NOT EXIST Warning
→ Perform scan
Cell temp set outside control range
18 LOW SET TEMP Warning
→ Change temp setting
D2 lamp not installed
19 NO D2 LAMP Alarm
→ D2 lamp disconnected or defective, PCB defect
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8.3 Problems with electrical circuits and points to check

Points to check on the SPD20-MAIN and SPD20-PWR (power supply) circuit
boards are summarized in the tables below. Please use them when experiencing
problems with printed circuit boards.
8.3.1 SPD20-MAIN circuit board

8.3.1.1 Reference voltages
Item Allowable range Points to check
+5V: +5 ±0.5V across P2-P40 ±12V
±A5V M10, M11, J19
-5V: -5 ±0.5V across P2-P41 → ±5V
+5 ±0.5V across P2-M1
D5V M7, L1, C12, C21
3.3V +3.3V ±0.3V across P2-P4 M5, C9, C17
24V +26V ±5.0V across P2-P3 M2, M4, M6, M7, M8, J19
18V +18V ±2V across P2-C3 J19
8.3.1.2 Firmware read circuits

Item Points to check
M21
J14, J15, J16, M14, M19. M21,
Failed to read (CPLD)
M32, X1
writing
8.3.1.3 Instrument startup circuits

Reset, NMI, CPU,
Instrument won’t start M18, M19, M25, M29, X1
RAM
Messages not displayed in VFD display area. Key M12, M17, M21, J9, F4 -- Key display
input not possible. LED display malfunctioning. F13 interface
8.3.1.4 PWR PCB interface circuit

D2 or W lamp does not turn on/off M12, M13, M19, J19
J19, M19, R32, R33, R39, D5 --
D2 or W lamp current values are abnormal
D8
Hg lamp does not turn on/off M1, J1, Q1, C3
J2 connector output voltage for PWR PCB not
J19, M12, M19
changing
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8.3.1.5 Motor drive and home position detection circuits

R/C around M2, M3, M4,
Grating motor does not turn (grating moves) M8, M27, M28, J8, RA8 --
RA10, M19, M2/4/8
Grating motor does not turn (no display) M14, M21, J4
Motor drive
Filter motor does not turn (filter moves) M9, M19, J7, Q2, Q3, R26
circuit
Detector circuit
Filter motor does not turn (no display) M14, M21, J4 M21 (CPLD)
writing
R/C around M3, M19, M6, Motor drive
Lamp motor does not turn (filter moves)
J6, M3/6 circuit
Detector circuit
Lamp motor does not turn (no display) M14, M21, J3 M21 (CPLD)
writing
8.3.1.6 Analog data output circuit

0V: 0 ±0.1 mV across R/C around M30, M31, J18,
Output value out of
J17-J18 J17, M30
range
100V: 100 ±0.1 mV R/F around C90/91
8.3.1.7 Detector input circuit and monitor input circuit

“SMPL EN”, ”REF SMPL EN = 2500 ±100 R/C around M13, M20, M22,
EN)” malfunction REF EN = 2500 ±100 M23, M24, M33, J13
Abnormal “LEAK
LEAK SENSE = 530 ±30
SENSE” or ”LEAK R/C around M16, J10
LEAK ROOM=255 ±30
ROOM” values
Abnormal
M34, R50, R102, R103, R105,
“RADIATOR T” RADIATOR T = 530 ±30
D22
values
8.3.1.8 Contact output and event input circuits

M14, M32, K1, K2, J16, R70,
Time program does not stop
R71, F18 -- F21
8.3.1.9 Cooling fan control circuit

Does not control M12, J19
8.3.1.10 Flow cell temperature-control circuit

Cell temp control
CELL TEMP=40 ±1 J19, M13, M19, R35, D7
display malfunction
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8.3.1.11 EEPROM writing and NMI function circuits

NOT PROTECTED occurs R/C around M29, M15, M29 NMI, EEPROM
Value settings are not recorded M15 EEPROM
8.3.1.12 HDLC transmission functions (optional) circuit

Cannot communicate with external
M14, M21, M26, J14, J15
devices
8.3.2 SPD20-PWR(POWER) circuit board

8.3.2.1 Reference voltages
+12V: +12 ±1V across P5-P8 C14, M10, M11, F5, F6, C46
±12V
-12V: -12 ±1V across P5-P9 -- C48, C51 -- C53, D13, D15
+5 ±0.5V across P1-M13 (8-pin)
D5V J6, M13, C59
+5 ±0.5V at C59, M13 (8-pin)
24V +26V±5V across P1-P7 D12, F3, F4, C44, C45
18V +18V±2V across P1-P10 M12, C57, C58
8.3.2.2 MAIN (CPU) PCB interface circuit

M5, M8, M9, Q5, L2, D16, R57,
Heater circuit
D2 lamp does not go R58
on/off M4, M6, K1, K2, R26, R27, F1,
Anode circuit
Q2, Q3
Abnormal [LAMP R/C around M4, M6, M8, Q2, Q3,
300 ±30
CUR] value F1, R26, R27, M8
Abnormal [D2 FIL
700 ±100 R/C around M8, R47, M8
CUR] value
W lamp does not go R/C around M2, M3, M5, Q4, L1,
on/off D11, R32, R33,R19, M2
Abnormal [W FIL
1500 ±200 R/C around M2, R19, M2
CUR] value
8.3.2.3 ecycle valve and VFC control circuits

5-8 pin voltage 13 ±3V M1, J2, R14, NF2 -- NF5
8.3.2.4 Cooling fan control circuit

Cannot control fan M12, J7, J9
8.3.2.5 Flow cell temperature control circuit

Abnormal temp R/C around M2, M13, Q7, J4, R65,
40 ±1C
display values M2/M13
49
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Chapter 9 Electrical circuits

9.1 PCB diagram, SPD-20 cables
50
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9.2 Circuit diagram, LC20-KEY
51
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9.3 Circuit diagram, SPD20-MAIN

9.3.1 Circuit diagram ,SPD20-MAIN 1/8
9.3.2
52
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9.3.2 Circuit diagram, SPD20-MAIN 2/8
53
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54
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55
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56
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57
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58
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59
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9.4 Circuit diagram, SPD- POWER

9.4.1 Circuit diagram ,SPD20-PWR 1/5
60
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9.4.2 Circuit diagram, SPD- POWER 2/5
61
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62
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63
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64
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9.5 Circuit diagram, PB-1 ASSY
65
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Chapter 10 Parts lists

10.1 Consumable parts list
No. Part name Part number Remarks
1 Deuterium lamp 228-34016-02 Light source
2 Tungsten halogen lamp 670-14602 Light source (SPD-20AV only)
3 Flow cell gasket 228-35097-01 Flow cell parts
4 Flow cell gasket 228-35097-05 Flow cell parts for UFLC model
5 Lens 228-14572 Flow cell parts
10.2 Optical repair parts list

1 Filter ASSY (VIS) 228-42066-91 W lamp heat-absorbing filter (SPD-20AV only)
2 Mirror M1 ASSY 228-23037-95
3 Mirror M2 ASSY 228-23014-91
4 Mirror M3 ASSY 228-23041-93
“-95” is previous model, with diffraction
Grating ASSY 228-15257-95
efficiency of 40% to 56%.
“-97” is a high-grade grating with diffraction

efficiency of 48% to 68％. It is installed on some
5 standard SPD-20A units as well, corresponding
Grating ASSY HI 228-15257-97 to the release timing of the UFLC models.
High-grade gratings can be used in instruments
that include the new type of printed circuit
board, SPD-20-MAIN ASSY (228-45601-94).
2nd-order light cut filter (for SPD-20A including

6 Filter ASSY (UV) 228-42008-91
UFLC model)
7 Filter ASSY (UV/VIS) 228-42008-92 2nd-order light cut filter (for SPD-20AV)
8 Motor, GR ASSY 228-23027-92 Grating drive motor
9 Photosensor “A” ASSY 228-25421-91 Sensor for mirror switching mechanism
10 Motor, M1 ASSY 228-42068-91 Motor for switching mirrors
11 Window 228-34795 Quartz window pane
12 Hg lamp ASSY 228-38214-96
13 Silicon photocell ASSY 228-23016-91 Silicon photodiode
14 PB-1 SPD20A ASSY 228-23691-95 Pre-amplifier
10.3 Temperature-controlled flow cell and plumbing parts list

Temperature-controlled
1 228-37440-94
flow cell ASSY 20A
Lens fastener screw
2 228-40239-91 Flow cell parts
ASSY
3 Tube holder 228-39621 Parts for fastening tubing
4 ETFE tubing 228-18495-06 1.6 O.D. × 0.3 I.D. mm
5 Male nut, PEEK 228-18565
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10.4 Electrical parts list

1 Fuse, 4AT, 250V 072-02004-22 100-120V fuse
2 Fuse, 3.15AT, 250V 072-02004-21 220-240V fuse
Main circuit board Without EEPROM.
PCB ASSY,
228-45601-92 SE and RE output is max 3.5 V.
SPD-20-MAIN(Old)
3
Main circuit board Without EEPROM.
PCB ASSY,
228-45601-94 SE and RE output is max 5 V. Order “-94”
SPD-20-MAIN(New)
for new order.
4 PCB ASSY, SPD-20-PWR 228-45602-91 Power supply circuit board
5 Switch ASSY, SPD-20 228-42047-91 Power switch
6 SPD-20 transformer ASSY 228-42040-02 Power supply transformer
7 Fan ASSY 228-42098-91 Rear fan
8 MD516B fan ASSY 228-42098-92 Rear fan
9 PCB ASSY, LC-20-KEY 228-45600-91 Keys
10 Leak sensor ASSY 228-39247-92 Leakage sensor
11 Filter, FN378-4151 075-00070-87 with input voltage selector
12 Panel ASSY 228-43200-95
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Chapter 11 Optional parts

11.1 Parts list and diagrams for optional parts
11.1.1 Temperature-controlled semi-micro cell (228-45605-94)
Refer to Sections 4.8.1 and 4.8.2 regarding the temperature-controlled flow
cell assembly
11.1.2 Inert cell (without temperature control) (228-33338-91)
No. Part Number Part Name No. Part Number Part Name
1 228-32982 Cell housing 14 228-33376-30 Tubing, PEEK 1.6 x 0.25
2 228-25440 Plate ”A” 15 026-02034 Spring pin, SST 2 x 8
Screw, SST sems P2 M3 x
3 228-32983 Angle 16 020-46140
20
4 228-32984 Plate 17 020-46535 Screw, SST sems P3 M3 x 8
5 228-14520 Cell fixing screw 18 020-46634 Screw, SST sems P4 M3 x 6
6 228-33347 Nut 21 228-23424 Cover, cell
7 228-14571 Gasket, PTFE 22 228-24181 Retaining bush
8 228-14572 Lens (R4, RD6) 23 228-32985 Cover
Screw, SST pan head
9 228-14569 Gasket 24 020-02906
M2 x 4
10 228-14568 Lens fixing screw 25 228-33255 Sponge
11 228-32998-02 Gasket, PTFE 6 x 2.5 x 0.2 26 228-31638 Ferrule 1.6F PEEK
12 228-25277-04 Label, inert 27 016-37722-06 FEP tube, NFL016
13 228-33376 Tubing, PEEK 1.6 x 0.25 28 016-37722-06 FEP tube, NFL016
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11.1.3 Preparative cell (variable capacity, without temperature control)

(228-23405-91(0.5)/92(0.2)/93(0.1))
No. Part Number Part Name -91 -92 -93

1 228-25287-91 Cell housing ASSY, prep
1 228-25287-92 Cell housing ASSY (0.5)
2 228-23421 Cell holder
3 228-23423 Plate, cell
4 228-23424 Cover, cell
5 228-14520 Cell fixing screw
6 228-21383 Gasket, PTFE
7 228-21384-02 Cell window
8 228-21385- Gasket -01 -02 -05
9 228-00147-02 Window, UV cell
10 228-21387 Gasket, cell window bracket
11 228-21386 Screw, cell window fixing
13 020-46548 Screw, SS sems P3 M4 x 10
14 020-46634 Screw, SS sems P4 M3 x 6
15 021-04633 Screw S WP M4 x 5
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11.2 Parts list and diagram for solvent recycle valve

(228-35310-91 45080-91)
No. Part number Part name

11 228-39732 Plate, SV
13 228-35364-91 Tube, inlet assembly
14 228-35365-91 Tube, outlet assembly
15 228-18565 Nut, male PEEK
18 228-35454-91 Valve, MRV 3-way
21 020-37054 Screw, SS truss head M4 x 8
22 020-46536 Screw, SS sems P3 M3 x 10
23 072-60319-01 Clamp, DKN-10GSP
24 228-35453 Cover, SV
26 075-08032-03 Filter core, EMI SFC-6
27 228-35673 Tray
28 228-35674-01 Spacer
29 228-35674-02 Spacer
30 228-30292 IM, recycle valve kit (JE)
31 037-02797-01 Knob, KDM-16-M4-10
32 228-25162-03 Tube, drain SI
70

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228-90095F

Chapter 3 Measurement systems....................................................................................................7

Chapter 4 Parts configuration and function...............................................................................13

Chapter 5 Other functions...............................................................................................................17

Chapter 6 Maintenance and adjustments..................................................................................22

6.1 Cleaning the temperature-controlled flow cell......................................................................................................22

Chapter 7 Additional explanations...............................................................................................36

7.2.12 Changing the power supply input voltage..................................................................................................44

Chapter 9 Electrical circuits............................................................................................................50

Chapter 10 Parts lists..........................................................................................................................66

Chapter 11 Optional parts.................................................................................................................68

E Revisions to “4.8 Temperature-controlled flow cell assembly drawing and

This manual is intended to explain the maintenance of 20A (including -20A

Chapter 3 Measurement systems

3.1.1 SPD-20AV Light path schematic

SPD-20AV Light path schematic

3.1.2 SPD-20A Light path schematic

SPD-20A Light path schematic

3.2 Electrical system

Schematic of signal processing circuits

3.2.2 Lamp power supply circuits

Fixed voltage Wlamp

Lamp illumination circuit schematic

Chapter 4 Parts configuration and function

4.2 Operation controls

4.4 Right and bottom sides

4.5 Back side

4.6 Inside the instrument

4.7 Inside the optical unit

Optical unit interior

4.8 Temperature-controlled flow cell drawing and component

4.8.2 Component parts list for temperature-controlled flow cell assembly

Chapter 5 Other functions

5.1.2 Control group

5.1.3 System group

5.1.4 Monitor group

5.2 List of VP functions

5.2.1 Product information group

5.2.3 Validation Support group

5.2.4 Calibration Support group

5.3 List of factory functions

Displays leak sensor room temperature

Chapter 6 Maintenance and adjustments

6.1.1 Simplified cleaning of temperature-controlled cell

6.1.2 Disassembled cleaning of flow cell

6.1.3 Assembling the flow cell

6.1.4 Cleaning the quartz window

6.3 Adjusting the optical system

6.3.3 Optical adjustment jigs (targets)

6.3.5 Adjusting slit position

6.3.6 Adjusting W lamp stopper screw

6.3.8 Adjusting the grating

6.3.10 Fine adjustment of mirror M1

6.3.11 Checking the filter position

6.3.12 Adjusting the Hg lamp

Chapter 7 Additional explanations

7.1 Differences from previous models

SPD-10A SPD-10Avp/ -20A Description

7.1.2 Clearing lamp usage times

7.1.3 Changing the gain of operation amplifier

7.1.3.1 Checking the light intensity

7.1.3.2 Procedure to check the light intensity

7.1.3.3 Changing the gain of pre-amplifier (PB-1 ASSY)