Experiment No.

6
Determination of Vitamin C in tablets

I2 + C4H6O4(OH)C=COH → 2I- + 2H+ + C4H6O4C(=O)-C=O

Procedure:

1. Weigh each 5 vitamin c tablet and determine the average mass of a single tablet.

2. Grind the tablets into fine powder using a mortar and pestle.

3. Weigh approximately 0.50 grams of vitamin c powder into three different Erlenmeyer
flasks labeled as trial 1, 2, and 3. Record the corresponding weights of each trial.

4. Add about 100 mL of water in each flask just before it is about to be titrated, followed by
one dropper full of starch indicator solution. Cover the opening of the flask with plastic
or foil and shake well to dissolve the sample completely. Replace the plastic or foil with a
piece of cardboard punched to admit the tip of your burette.

5. Insert the tip of the buret through the cardboard covering the mouth of the flask and
titrate with standard iodine solution with deliberate speed until the first permanent
appearance of the blue iodo-starch color.

6. Repeat the titration (steps 4 and 5) for trials 2 and 3.

7. Calculate the percentage of Vitamin C, C6H8O6, in your sample.

Reference: Adapted from Fritz and Schenk, Quantitative Chemical Analysis

Vitamin C analysis (experiment). Retrieve from https://chem.libretexts.org/Ancillary_Materials/
Laboratory_Experiments/ Wet_Lab_Experiments/General_Chemistry_Labs/ Online_Chemistry_Lab_
Manual/Chem_11_Experiments/10%3A_Vitamin_C_Analysis_(Experiment)
 Experiment No. 6
Determination of Vitamin C in tablets

Trial 1 Trial 2 Trial 3

Weight of paper + vitamin c powder
sample (g)
Weight of paper after transferring of
vitamin c powder sample (g)
Weight of vitamin c powder sample (g)
Final Volume of I2 (mL)
Initial Volume of I2 (mL)
Volume of I2 Used (mL)
Concentration of I2 (M)
% C6H8O6
Average % C6H8O6
% Labelled claim

Calculations:

Reference: Adapted from Fritz and Schenk, Quantitative Chemical Analysis

Vitamin C analysis (experiment). Retrieve from https://chem.libretexts.org/Ancillary_Materials/
Laboratory_Experiments/ Wet_Lab_Experiments/General_Chemistry_Labs/ Online_Chemistry_Lab_
Manual/Chem_11_Experiments/10%3A_Vitamin_C_Analysis_(Experiment)