Section IT BACTERIOLOGY and ALLIED FIELDS Editor: James I, Mangels, Laboratory Manager, Clinical Migrabology Stanford University Hospi ‘Stanford, California226 Exam Questions, Bacteriology & Allied Fields INTRODUCTION : 1. Circle the following term(s) to describe an eucaryotic cell: a. Nuclear apparatus b. True nucleus ¢. Membrane-bound organelles dL Semi-tigid cell wall 2 Which of the following is not a procaryote? a. Bluegreen algae b. Protozoa ©. Bacteria Rickettsiae 3. Autotrophic organisms require inorganic matter for energy T,F . Which of the terms of the normal growth cycle best ‘ describes a stage when bacteria are adapting to a new environment? a. Lag phase bag Brexponentl phase ¢. Stationary phase Phase of decline 5. Circle the following structures in procaryotic cells: a. Cell wall b. Cytoplasmic membrane ¢. Ribosomes d Pili 6. Which feature best describes the log or exponential phase? 8. Organisms adapt to a new environment b. The accumulation of toxic waste causes a decrease in the rate of growth ¢. Cells divide at a constant rate d The number of viable bacteria begins to decrease 7, An organism which grows on a blood agar plate incubated in a candle jar as well as on a duplicate blood agar plate incubated under strict anaerobic conditions; but will not grow on a triplicate blood agar plate incubated aerobically should be called: a. Astrict anaerobe b. Microphilic ©. Capnophilic dA facultative anaerobe b Vol. I, p 1 b Vol. 1, p. 1 T Vol. I, p. 1 a Vol. 1, p. 2 abd Vol.'t, p. 1 c Vol. I, p. 2 b Vol. I, p. 3 10. 12. 13. 14, Exam Questions, Bacteriology & Allied Fields 227 ‘The term that best describes the location of flagella which entirely surrounds the bacteria is: a. Atrichous b. Monotrichous ¢. Amphitrichous d.Peritrichous Vol. I, p. 3 ‘The thermal requirement that best describes the ‘majoity of pathogenic bacteria is called: a. Mesophilic b. Psychrophilic . . &. Thermophilic d Heterotrophic Bacterial cells of one species which do vary in thir iz and arrangement are std tobe: a ic b. Metamorphic &. Polymorphic 4. Pleomorphic ‘Mycoplasma differ from bacteria in that they: a. Are thermophilic Lack a rigid cell wall Are pleomorphic and more sen: ‘osmotic pressure Need serum for growth a Vol. 1, p. 3 c Vol. 1, p. 3, 134 to changes in Vol. I, p. 3 In the phase of decline the total number of organisms: a. Increase in number b. Decrease in number ¢. Remain the same G 4 Adapt to new environment Vol. I. p. 2 A characteristic that separates Mycoplasma from bacteri a. Does not contain RNA b. Lacks a cell membrane ©. Lacks a cell wall Requires living cells for growth © Vol. I, p. 3 Viruses contain: a. Both DNA and RNA. b. Neither DNA or RNA ¢. Either DNA or RNA. dA polysaccharide shell Vol. I, p. 4228 Exam Questions, Bacteriology & Allied Fields Exam Questions, Bacteriology & Allied Fields 229 f the following is a characteristic of Rickettsiae’ 22. If we know that an organism ferments lactose, then 15, Which ofthe following i ctr cell wal far as glucose is concerned, itis safe to say that: 5. Gram-negative obligate, intracellular parasites a. It probably will be fermented ‘c. Considered to be among the viruses b a b. It defintlely will be fermented 4 Contain only RNA Vol. 1, p. c. It definitely will not be fermented b ve f medical d Itprobably will not be fermented Vol. I p. 14 Order Spirochetales, three genera of medic . oe 16. soon the ae me 23. Which of the following methods would best sterilize Pe somelia, Treponema, and Leptospira a solution of lactose without hydrolyzing it? b Fordella, Borrelia and Chlamydiae a. Filtering it through a bacterial filter c. Leptospria, Borrelia, and Rickettsiae s Lip b. Autoclaving it at 15 Ibs, for 20 minutes 4. Rickettsiae, Chlamydiae, and Leptospira, Vol. I p. ©. Boiling for 30 minutes * a d. Freeze-thaw 3 times Vol. I, p. 5-6 17- Which of the following best describes viruses? o i ‘a. Possess RNA onl} od An organism NOT generally recovered from spinal fluid] 'b. Dependent upon living cells for their growth ar a. Beta hemotytic streptococci multiplication 6 b. Neisseria meninigitis cc. Multiply by binary fission Vol tp. 4 ©. Bacteroides spp. 4 d_ Possess-ribosomes . Haemophilus influenzae Vol. I, p. 8 ic structural unit of fungi are filaments called 18. aes ‘of hyphae is 25. prctganism NOT generally recovered from deep wound . Nonseptate a a. Coliforms Aerial hyphae b. Staphylococcus aureus » @. Mycelium Vol. 1 pA Haemophilus influenzae lc Special hyphae of fungi which contain spores are called Bacteroides spp. Vol. Lp. 9 19. Speci 26. Gram stained smears to determine morphology : ane baa and staining characteristics should be made from: ‘ one b a. Any age culture on any medium & iydospores Vol. I, p.5 b. Single colony pickings made from any medium ‘Arthrospores e Young, actively growing ‘organisms grown 20. Tyndallization is a process of fractional discontinous 4 Oldcuttures on rich medium fs sterilization which includes: —— 7 Vol. I, p. 28 ‘a. 100°C for 30 minutes on 3 successive Biochemical studies should be performed using: b. 60°C for 1 hour on two different days. . ‘Suspensions of several be perk med using i. 37°C for 30 minutes twice a day es Any type of culture of any age d.. 100°C for 5 minutes on two successi ye days. fol. I, P. Any so long as Gram-stained smear does not show organisms having different 21. Cellulose acetate filtration is generally used for one morphology or staining characteristics of the following sets? Young, actively growing cultures made from d ‘a. Carbohydrates, albumin, protein single colony pickings Vol. I, p 14 b. oe areal Fy 28. Lines - following is not true about the standard e fluids, plate count’ ‘Protein, organic material, sera Vol. I, p.6 a. Counts all viable organism 'b. Accurate for samples with low bacterial counts' 230 Exam Questions, Bacteriology & Allied Fields 29. 30. 31. 32. 33. 34. cc. Samples with high populations can be diluted 4 bef latins a Colonies canbe accurately counted Vol. I, p. 124 'Name, in order, the four ingredients of Hucker's odified Gram sti 2. Ceystal violet, 95% alcohol, iodine, safranin B._ Todine crystal violet, 95% alcohol, safan . Crystal violet, 95% alcohol, safranin, iodine Crystal violet, iodine, 95% alcohol, safranin Organisms which can utilize organic materials as a source of energy are called: a. Heterotrophic b. Holozoic ¢. Autotrophic a Saprophytic ‘The principle of the autoclave for sterilization is based upon: ‘Dry heat with pressure of 15 Ibs. at 120°C for Molt estat 1209C for 15 minutes ‘Moist heat at 120°C for 15 mim Moist heat at 120°C, under pressure of 15 Ibs. for 15 minutes Dry heat at 120°C, for 15 minutes Disinfection of environmental surfaces destroyes ‘microorganisms, but not: a: Staphylococcus aureus b. Anaerobes | Resistant spores @ Salmonella typhi Pasteurization is a process in which: 2. All organisms are destroyed by heating at 62°C for 30 minutes ey ia b. All organisms are removed a € The total bacterial count is lowered by irradiation d° The total bacterial count is lowered by heating, at 62°C for 30 minutes ‘Two methods which may be used to determine motility are: Sg a. Hanging drop and motility mediim b. Hanging drop and Gram stain cc, Motility medium and simple stain 4 Gram stain and motility medium a Vol. I, p. a Vol. 1, p. 5 oo oP lc Vol. I, p. 5 & © Vol. 1, p. 6 4 Vol. 1, p. 6 a Vol. I, p.7 35. 36. 37, 38. 39. 40. 41 42. - Exam Questions, Bacteriology & Allied Fields 231 ‘The growth phase in which microorganisms are ‘ost susceptible to antimicrobial agents is: b. Stationary phase ©. Decline phase Exponential phase ‘What color would a Gram positive bacterium be if the bacteriologist forgot to use the mordant during the Gram stain procedures? a. Pink b. Purple ¢. Nocolor Pink and purple ‘Which of the following genera is Gram-positive? a. Neisseria — aa b. Staphylococcus c: Veillonella d Branhamella ‘Sodium desoxycholate inhibits the growth of: a. Gram-positive organisms b. Gram-negative organisms Pasteurization of milk is usually carried o1 a. $4°C for 40 minutes at b. 63°C for 30 minutes ¢. 70°C for 70 minutes 4 82°C for 45 minutes GENERAL MEDICAL BACTERIOLOGY Which of the following genera is Gram-positive? a. Neisseria b. Staphylococcus ©. Branhamella Actinobacillus ‘The difference(s) between “aerobic” organisms and "microserophilic” organisms are: Nutritional requirements Grouping morphology ¢. ‘Temperature requirements Oxygen requirements ‘Sugars, serum, and tissue extracts should be sterilized a.” Heat without pressure b. Special chemicals Vol. I, p. 2 Vol. I, p. 29 b Vol. I, p. 14-16 a Vol. I, p. 37 Vol. 1, p. 8-10 d Vol. I, p. 3 |232. Exam Questions, Bacteriology & Allied Fields 43. 44, 45. 46. 47. 48. 50. c. Filtration None of the above What is the single specific growth necessary for the cultivation of Franc a. Cystine b. 10% carbon dioxide ‘What isthe single specific growth requirement necessary for the cultivation of Brucella abortus? a. 10% carbon dioxide b. 10% serum nt lla tularensis ? ‘What is the single specific growth requirement necessary for the cultivation of Clostridium perfringens a. Cystine (8) Anaerobic conditions ‘Which organism is best particularly related to the statement of "frequent cause of meningitis"? Which organism is best particularly related to the of "may be found in raw milk"? a Brucella . ~B pertussis ce. F.tularensis a Y. pestis Which organism is best particularly related to the statement of, "spread from animal host to man by arthropod vector"? a. Brucella b. B. permussis ©. ¥. pestis dF tularensis Which organism is best particularly related to the statement of the “cause of whooping cough”? a. F. tularensis b. Borrelia Brucella Bordetella pertussis ‘Which organism is best particularly related to the statement of "mal reservoir of infection found in c Vol. I, p. 6 a Vol. I, p. 70 a Vol. I, p. 37 Vol. I, p. 18 ¢ Vol. I, p. 8 a Vol. I, p. 8 c Vol. 1, p. 8 id Vol. I, p. 8 51. 52. 53. 34, 55. 56. Exam Questions, Bacteriology & Allied Fields 2: wild rabbits"? SH. influenzae a Y. pestis Which organism is not commonly recovered from spinal fluid? (a! Neisseria gonorrhoeae Neisseria meningitidis €. Streptococcus pneuroniae @ Haemophilus influenzae type b. ‘Which organism is not commonly recovered from infected urine? Yersinia ‘Pseudomonas ‘Staphylococcus spp can be differentiated from Micrococcus spp by their ability to: a. Oxidize glucose rather than ferment Ferment glucose rather than oxidize . Catalase reaction Ferment mannitol rather than oxidize Which statement is correct: Staphylococcus aureus can be differentiated from Staphylococcus epidermidis by their ability a. Catalase positive, ferment mannitol, and are DNase tegative b, Produces clumping factor, does not ferment mannitol, and are DNase positive ¢. Coagulase positive, DNase positive, and is catalase negative Ferment mannitol, produces clumping factor, and is DNase positive Strains of S. aureus may produce an exfoliation toxin causing: a. Scalded-skin syndrome b. Erysipelas c. Scarlet fever d Rheumatic fever Vol. I, p. 70 a Vol. I, p. 8 © Vol. I, p. 9 Vol. I, p. 11 4 Vol. I, p. 11 a Vol. I, p11 ‘Streptococcus spp may be differentiated from ‘Staphyloccus spp by: a. Hemolysis234 Exam Questions, Bacteriology & Allied Fields b. Catalase negative cc. Catalase positive d. Fermentation of mannitol 57.» Which is not associated with beta hemolytic streptococci? a. Scalded-skin syndrome Rheumatic fever c. Exysipelas Scarlet fever 58. Beta hemolytic streptococci are divided into different Lancefield groups based upon: a. Hemolytic patterns b.. Biochemical differences ; c. Group specific cell wall polysaccharide antigen Type specific protein cell wall antigens 59. Which characteristic is associated with Streptococcus pneumoniae? a. Gram-negative lancet-shaped diplococci . Bacitracin positive ¢. Inulin negative d._Ethylhydrocupreine hydrochloride positive 60. _. Which is not a characteristic of Neisseria gonorrhoeae? a. Gram-negative diplococei b. Growth is improved withS-8% CO, c. Oxidase positive Anaerobic growth requirement 61. Which set of biochemical reactions best fits the typical pattern of Escherichia coli? i a. Indole positive, lysine decarboxylase positive, motile, KCN positive b. Indole positive, citrate positive, lysine decarboxy- lase negative, motile ¢. Indole positive, KCN negative, citrate positive, lysine decarboxylase positive Indole positive, lysine decarboxylase negative, KCN negative, citrate negative». . Some strains of E. coli may be enteropathogenic, 62 Mfc oat est may be used to identity these stains? a. Lactose not fermented b. Lactose fermented Q Sertitl fermented Sucrose negative > Vol. I, p. 11 Vol. I, p. 12 c Vol. I, p, 12 4 Vol. I, p. 12 a Vol. I, p. 13 Vol. 1, p. 14 © Vol. I, p. 14 63. 64, 65. 66. 67. Exam Questions, Bacteriology & Allied Fields 235 ‘Which set of biochemical reactions best fits the typical pattern of Shigella? a. VP positive, citrate positive, lysine negative, ves, KCN negative (CD. VP negative, citrate negative, lysine negative, KCN negative ©. VP positive, citrate negative, lysine positive, KCN negative 4 VP negative, citrate negative, lysine positive, KCN negative ‘Which best series of reactions can be used.to separate Edwardsiella from Shigella? a." Hydrogen sulfide positive, positive for motility, lysine decarboxylase negative ‘Hydrogen sulfide negative, non-motile, lysine decarboxylase positive © Hydrogen sulfide positive, positive for motility, lysine decarboxylase positive 4 Hydrogen sulfide negative, positive for motility, indole negative, lysine negative ‘Which best series of reactions can be used to identify Salmonella spp? 1. Citrate positive, urease negative, KCN negative, lysine decarboxylase positive b. Citrate negative, urease negative, KCN positive, lysine decarboxylase negative ~ itrate positive, urease negative, KCN positive, lysine decarboxylase positive Citrate negative, VP positive, KCN negative, lysine decarboxylase positive ‘Which best series of reactions can be used to separate Citrobacter from Salmonella? : a. "Lysine positive, KCN positive, urease positive, positive for motility » Lysine negative, KCN positive, urease negative, positive for motility ce. Lysin KCN negative, urease negative, positive for motility d._zLysine positive, KCN negative, urease Positive, positive for motility Which is the best series of characteristics to deseribe Pseudomonas aeruginosa? Blue-green pigment in media, oxidase positive, ~ _ grape-like odor b. Oxidase negative, ferments glucose, blue-green Vol. 1, p.14, c Vol. I, p. 14 © Vol. I, p. 14 Vol. I, p. 15236 ‘Exam Questions, Bacteriology & Allied Fields pigment in media c. Grape-like odor, brown pigment, oxidase negative Oxidase positive non-motile, grape-like odor, no pigment in medi animals, but may be isolated in man from the followin, sites: ja, Urinay trac infecions | Animal bites ©) Wound infections Ring worm infections 68. Pasteurella multocida is primarily a pathogen of lower | 69. Which series of characteristics can be best used to identify Pasteurella multocida? a, Motility, bipolor staining 'b. Gram-negative pleomorphic coccus ‘¢) Non-motile, gram-negative pleomorphic rods &._Non-motile, gram-positive pleomorphic rods 70. Francisella tularensis which is the etiologic agent of tularemia in man, differs from other bacteria in that they divide by: S a. Fusion a b. Fission ‘c) Budding Conjugation 71. Bordetella pertussis have been traditionally isolated from a medium requiring potato-blood-glycerol agar, however, a more recent medium containing charcoal blood agar with antibiotics is called: : a. Bordet-Gengou medium b. Cough plate ¢. Brucella ©), Regan-Lowe 72. Undulant fever in man is due to: a, Bordetella b. Francisella c.” Malaria d Brucella 73, The three species of Brucella are commonly differentiated by which series of tests? fa. Oxygen requirement, basic fushin, and coenzyme I b. Carbon dioxide requirement, thionine, and X factor ¢. Microaerophilic requirement, d) Carbon dioxide requirement, basic fushin, thionine , basic fushin a Vol. I, p. 15 be Vol. I, p. 16 c Vol. 1, p. 16 © Vol. I, p. 16 d Vol. I, p. 16 a ‘Vol. 1, p. 16 Exam Questions, Bacteriology & Allied Fields 237 4. ‘of Haemophilus are commonly differentiated g 15. Haemophilus influenzae is the most cause of meninigitis in children aged 1-5 ont F ¥ ¢. Staphylococci produce acid from glucose while BEPC tint coon saphyococel poduostager esas 77. Members of the genus Micr iter x fom gh oe ‘do not ) Produce aci in the | of Produce acid eter inthe presence ox aboence of oxygen ¢. Produce both acid and gas Produce acid only in the absence of oxygen a Vol. I, p. 13 78. Staphylococcus spp. can be differentiated from icrococcus spp. by: Cal OF glucose test 9. Hemolysis on a blood agar plate ¢. Fermentation of manniel : Tube coagulase test a Vol. 1, p. 13 79. Staphylococcus spp. which are etiologi foe are etiologic agents of a. Are easly differentiated, morphologically, from other members of the genus Staphylococcus ®) Produce a characteristic enterotoxin . Are members of the enterococci group b Produce a characteristic erythrogenic toxin Vol. I, p. 11 80. A Gram-positive cocct blood. Gulsre bot. “Tho blochomical reatone were catalase, fermented glucose, clumping facto, soluble238 Exam Questions, Bacteriology & Allied Fields ‘coagulase - after incubation overnight in 37°C water bath. The technolgist should: a Report S. Epidermidis isolated b. Repeat the OF glucose test c. Request another specimen Repeat the coagulase test 81. The catalase test readily differentiates members of genera Micrococcus and Staphylococcus from Streptococcus. What would cause a false negative test? a. Blood cells or tissue cells are present BL Media lacking blood c. Media rich in reducing substances Improper temperature, 82. ‘The plasma of choice for the coagulase test is: a. Sheep plasma b. Human plasma ¢. Rabbit plasma Rabbit serum 83. A gram-stained smear from colonies on an aerobic bl agar plate shows an encapsulated Gram-positive cocci grouped in tetrads. ‘The organism is: a. Streptococcus pneumoniae b. A staphylococcus ¢. A streptococcus Micrococcus tetragena 84, The OF glucose test differentiates between the genus Micrococcus and Staphylococcus because: a. Micrococcus only ferments glucose b. Micrococcus only oxidizes glucose ¢. Micrococcus ferments as well as oxidizes glucose ‘medium . Micrococcus doesn't grow in OF glucose medium 85. AGram-stained smear from colonies on an aerobic blood agar plate shows Gram-positive cocci grouped in packets of.cight-or-more-cells. The organism is probably: a. Micrococcus tetragena b. Neisseria meningitidis ¢. Micrococcus luteus Streptococcus pneumoniae 86, The hydrogen peroxide used in the catalase test should be stored at Vol. I, p. 52 © Vol. 1, p. $1 c Vol. I, p. 51-52 a Vol. I, p. 13 b Vol., I, p. 13 c Vol. I, p. 13 87. 88. 89. 91. 92. tolerance ©. Bacitracin resistance a Bile solubility Vol. I, p. 58 ‘When pneumococci are mixed with specific immune serum there occurs, in addition to agglutination, a swelling ofthe cell capsules, This swelling “quellung” non is the: ca) "Neufeids reaction b Freund's reaction ¢. Weil-Felix reaction a d Cross-reaction Vol. I, p. 13 Streptococcus pneumoniae is also known as: a. "Walking pneumonia” b. "Crib death” ©. "Child bed fever" 4 4) The pneumococcus Vol. 1, p. 12 Streptococcus pneumoniae may cause: a. Pneumonia242 Exam Questions, Bacteriology & Allied Fields b. Meningitis ©. Pericarditis d Endocarditis ¢/” All of the above 106. The gonococcus can best be differentiated from ‘meningococcus by: a. Fermentation of dextrose and lactose b. Fermentation of dextrose and maltose £. Fermentation of maltose and lactose ‘Fermentation of glucose only 107. Presumptive identification of N. gonorrhoeae, based only upon finding oxidase positive colonies is subject toa high rate of false-positive diagnosis. What are two other organisms which might be mistaken for gonorrhoeae upon this basis alone: a. Pseudomonas and Acinetobacter wofft 'N. lactamica and Micrococcus (¢) Acinetobacter lwoffi and Moraxella Moraxella and Pseudomonas 108. Ne conditions to recover N. gonorrhoeae are: a. 35°C, CO? incubator or candle jar, on sheep blood agar b. 35°C, aerobic incubator, on G.C chocolate agar ¢. 350C, anaerobic jar, on G.C chocolate agar 4. 35°C; CO2 incubator or candle jar, on G.C. chocolate agar 109. Whats the preferred reagent to perform oxidase testing on N. gonorrhoeae: t. Pireclimettyl cnsamalichye b. Ethyl ‘upreine hydrochloride p Opuehin hye d.) P-aminodimethylamine monobydrochloride 110. If positive, the oxidase test on colonies containing Gram-negative diplococci will identify: a. iasa genus b. Only NW, gonorrhoeae ©. Only N. gonorrhoeae and N. meningitidis dN. gonorrhoeae but not N. meningitidis 111. Areport of N. gonorrhoeae preseiit depends upon the finding of: a. Gram-negative diplococci intracellularly in exudates} b. Oxidase-positive colonies in culture e Vol. I, p. 12 d Vol. I, p. 59 ¢ Vol. I, p. 60 d Vol. I, p. 59 Vol. 1. p. 59 a Vol. I, p. 59 c. A pure culture of Gram-negative diplococci 112, 113. 114. 1s. 116. Exam Questions, Bacteriology & Allied Fields 243 hich biochemical reactions typical of which produce bi typical of i © 4 Coagulase-positive diplococci Vol. 1, p.'59 Neisseria gonorrhoeae grows best when incubated: a, Aerobically b. Under 10% CO2 ©. Under 10% NO b d Anaerobically Vol. I, p. 59 ENTEROBACTERIACEAE Enteric pathogens associated with food poisoning epidemics, in the Salmonella and Shigella group, are: a) Gram-negative bacilli which will ferment glucose with or without gas b. Gram-positive bacilli which will ferment glucose with or without gas ©. Gram-negative bacilli which will ferment lactose with or without gas Gram-negative bacilli which will ferment sucrose with or without gas Trep peli iminaty identification of Enterobacteriaceae is made by: a. Biochemical methods b. Serological methods ©. Director mi ic examination Extreme resistance to antibacterial agents a Vol. I, p. 60 a ‘Vol. I, p. 60 ‘An enteric organism which ferments dextrose with gas, grows in KCN broth, does not ferment lactose or ‘Sucrose, and gives a negative reaction on urea agar after 24 hours incubation would probably be classified as: a. Citrobacter b. Salmonella ©. Shigella d Proteus a ©. Pseudomonas Vol. I, p. 62 A non-motile Gram negative rod which ferments dex- ttose without gas, does not ferment mannitol or produce} HS, and is citrate positive, is most likely to be: a. Salmonella typhi b. Shigella dysenteriae Salmonella pararyphi A b ‘Salmonella paratyphi B Vol. I, p. 61244 Exam Questions, Bacteriology & Allied Fields 117. 118. 119, 120. 121 122. 123. Nutrient broth should not be substituted for peptone broth when testing for indol because nutrient broth does} T. ‘not contain sufficient amounts of tryptophane. T, F ‘A principal characteristic used in the laboratory for distinguishing intestinal pathogens from other enterobacteriaceae is: a. Mannitol fermentation b. Production of acetyl-methyl-carbinol ¢. Animal pathogenicity ~ Rate of growth €. Lactose fermentation Indol formation and a positive methyl red test are diagnostic tests that aid in the differentiation of fecal coliforms from non-fecal coliforms. T, F Ina search for carriers of Salmonella typhi, the plating medium of choice i a SS b. BS c. BG d EMB e. DCLS Ina search for cases of Shigellosis, the plating media of choice are: a. EMB and SS b. SSand BS : . BG and MacConkey's and BS BGand BS . BS and MacConkey's, Mannitol is used in biochemical studies of suspected enteric pathogens in order to: a. Determine indol production b. Test for the presence of the enzyme urease ¢. Exclude possible Shigella-like organisms as non-pathogens 4. Classify possible salmonellas tentatively into smaller groups and exclude or confirm possible shigellas €. Confirm or eliminate salmonella-like organisms and to subdivide shigella into smaller groups Members of the shigella group react to produce an alkaline slant and acid butt when grown on TS.L medium because they ferment: a. Sucrose Vol. I, p. 68 e Vol. I, p. 60-61 T Vol. I, p. 64 Vol. I, p. 61 a Vol. I, p. 61 e Vol. I, p. 60 124. 125, 126. 127. 128. 129, Exam Questions, Bacteriology & Allied Fields 24! A typical aerogenic Salmonella strain, grown on TSI medium, will show: a line slant, acid butt, no gas HoS b. Alkaline slant, alkaline but, no Hz ¢. Acid slant, acid butt, gas, H2S Alkaline siant, acid butt, gas, HS Acid slant, alRaline butt, gas, HS e A pure culture of a Gram-negative bacillus isolated fro ‘food infection specimen may be excluded from funher ly as an enteric pathogen when after 24 hours incubation at 37°C it ferments: a. Mannitol b. Glucose Sucrose Dextrose ‘Which of the following reactions will be produced bj Shigella dysenteriae in a slant of TSI? y a. Neutral or alkaline butt, acid slant Acid butt, neutral or alkaline slant, no gas, no H2S| ¢. Acid and gas in both the butt and the slant 4 Acid and gas in the butt, neutral or alkaline slant All members of the family Enterobacteriaceae ferment: a b. Dulcitol ©. Glucose a e ‘The urea in Christensen’s agar is hydrolyzed in three mare 's agar is hydrolyzed in a. Salmonella typhi Shigella boy ¢. Shigella flexneri Proteus vulgaris €. Salmonella enteritidis vat, enteritidis ‘The organism, isolated from stool specimens, which ‘may be excluded from further study as a possible ¢ Vol. 1, p. 60 d Vol. I, p. 38, 61 c Vol. I, p. 62 ¥. ol. I, p. 38, 60 ¢ Vol. I, p. 60 d Vol. I, p. 60, 61 enteric pathogen by a positive reaction on urea is: a. Ancona hinshawil . b. Providencia stwarti246 Exam Questions, Bacteriology & Allied Fields c. Strep. fecalis d. Alcaligenes fecalis . Proteus 130. The production of phenylpyruvic acid from phenyl- alanine is useful inthe identification of: a. Klebsiella pneumoniae b. Klebsiella ozaenae ©. Salmonella @ Providencia e. Escherichia coli 131, Salmonella typhi is the only member of the ‘Salmonella group D that: ‘a. Contains flagellar d antigen b. Ferments glucose with the production of gas. . Forms indo! Will grow on Simmon’s citrate agar ‘e. Ferments lactose without producing gas 132. In the grouping of Shigella organisms, aggluti- nation by group C antiserum indicates that species is: a. Dysenteriae b. Boydii c. Flexneri dd Sonnei 133.’ ‘The single biochemical test which best differentiates Proteus from Salmonella and Shigella is. urease. T, F 134, If no red color develops after the addition of zine dust in the nitrate reduction test, it is safe to conclude that the nitrate testis negative. T, F 135, ‘The production of acetylmethylcarbinol from dextrose constitutes the basis for the following test: a. Neufeld-Quellung b. Cholera red c. Voges-Proskauer Oxidase 136. Sodium citrate is utilized by Escherichia coli as'the source of carbon in Simmon's citrate. T, F e Vol. I, p. 63 4 Vol. I, p. 69 a Vol. I, p. 62 b Vol. I, p. 60 T Vol. I, p. 63 F Vol. I, p. 68 © ‘Vol. I, p. 69 F Vol. I, p. 61 137. A positive test using Simmons citrate agar slants, showing alkaline reaction and utilization of citrate, ‘would be indicated by the following color: a. Orange 138. 139. 140. 141. 142 143. Exam Questions, Bacteriology & Allied Fields 247 b. Yellow ©. Red a a Blue Vol. I, p. 61 A gram-negative rod i isolated on EMB. Itis citrate positive ani reaction is A/AG with sulfide. It probably is: =e a. Klebsiella pneumoniae b. Salmonelia typhi ©. Edwardsiella tarda d Enteropathogenic E. coli ‘The indicator employed in TSI agar and urease test medium is: a. Phenol red b. Bromcresol le cc. Bromthymol blue d Neutral red a Vol. I, p. 62 a Vol. 1, p. 38 A single tube differential medium, employed for the identification of enteric pathogens and serving as an indicator of lactose, sucrose, and glucose fermentation as well as HS production a. Triple sugar agar b. Triple sugar-iron (TSD) agar ¢. Russell's double sugar (RDS) b d._Kligler’s iron agar (KIA) Vol. I, p. 38 Enteric pathogens (Shigella-Salmonella) are most easil differentiated from normal intestinal Gram | by their failure to ferment: =| Glucose ‘Mannitol Xylose 4 —— Vol. I, p. 60, 61 ‘Shigella sonnei ferment lactose: a ly b. Slowly Rose b sc Lp. 14, a 1 Citrobacter freundii may be difficult to differentiate from te following bacause i produces HS: Salmone rizona groups b. Edwardsiella group ©. Enterobacter grou 4. Serratia-Klebsiella group Vol. p. 61248 Exam Questions, Bacteriology & Allied Fields 144. 145. 146. 147. 148. 149. 150. ‘A Gram-negative, non-motile, glucose fermenting, lactose non-fermenting, urease negative, but phenylal- ‘nine deaminase + rod isolated on MacConkey is probably: a. E,hafnia b. Arizona hinshawii ©. Enterobacter liquifacicus d. Providencia spp. Because Citrobacter spp. share common antigens Salmonella spp. false positive agglutination may occur in polyvalent Salmonella antisera. T, F ‘On desoxycholate, desoxycholate-citrate, EMB, SS, and MacConkey agar the members ofthe Shigella Salmonella group produce small colonies which are: a. Black b. Green Pink d- Colorless In desoxycholate-citrate agar (a selective medium for the enteric pathogens), the purpose of the ferric ammonium citrate is to inhibit the growth of: a. The Gram-positive organisms : b. Escherichia eoli and other Gram-negative rods that predominate in the normal intestine For the isolation of Salmonella typhi the best single ‘medium is: ‘a, Eosin-methylene blue (EMB) agar b.. Endo —— ¢. Bismuth-sulfite (BS) agar (Wilson-Blair medium) d MacConkey agar ‘On BS agar the colonies of Salmonella typhi are ccharacteristically: a. Pink b. Black with metallic silver sheen ©. Green d Colorless The following organisms may be isolated from the urine in infections: Proteus 6. Candida albicans ©. Mycobacterium tuberculosis 4 Vol. I, p. 63 T Vol. I, p. 62 4 Vol. I, p. 38 b Vol. I, p. 38 © Vol. I, p. 38, 61 b Vol. I, p. 38 d Salmonella 151. 152. 153. 154, 155. 156. 157. + Exam Questions, Bacteriology & Allied Fields 24s ‘The most common cause of urinary tract infection is: a. Streptococci b. Staphylococci ©. Coliforms d.Diphtheroids ‘Salmonella spp. can readily be differentiated from ‘Shigella spp. because Salmonella spp. are: ‘a. Motile except for 2 species 'b. Produce phenylalanine deaminase c. Citrate positive. > All hydrogen sulfide positive ‘The basis of the Methyl Red test, used in differentiating Escherichia coli from Enterobacter aerogenes, is the production of: a. Citric acid b. Abigh acidity ©. Acetylmethylcarbinol Hydrogen sulfide MYCOBACTERIA ‘The N-acetyl-L-cysteine component of the NALC- NaOH reagent for the treatment of sputum specimens prior to culture has the function of: a. Killing mold contaminants : b. Decontamination of the specimen permitting use of less concentrated NaOH for digestion c. Liquefaction of mucus dDetoxifying NaOH ‘The granules observed in acid-fast stains of smears Spntaining M. tuberculosis ae called "Much’s granules One of the digesting reagents used for the concentration of the tubercle bacillus in sputum contains: a. Bromthymol blue b. Sodium hydroxide ¢. Potassium alum d Allofthe above ‘Mycobacterium leprae is included with the mycobacterial because: a. Of their acid-fast staining characteristics and thei morphological resemblance to the tubercle bacilli c Vol. Lp. 9 a Vol. I, p. 60 b ‘Vol. I, p. 68 . They are motile and produce spores ¢. Both Mycobacterium tuberculosis and M. leprae250 Bacteriology & Allied Fields 158. 159. 160, 161. 162 163. ‘were discovered by Hansen 4. All of the above ‘granular, thick, id q brittl ‘Acid-fast bacilli producing a peers lowish, growth with a wrinkled Weeks most probably is: a. M. tuberculosis b. M. bouis cc. Aphotochromogen d. .Ascotochromogen Concentration methods for acid-fast bacilli include a. Digestion of mucus with alkali b. Digestion with bleach cc. Centrifugation d Allof the above id-fast bacilli classified as scotochromogens, Photechromogens and rapid growers differ, in general rom M. tuberculosis in that they: a. Are nonpathogenic for man b. Do not produce niacin c it pec ‘media for growth Are susceptible to all antitubercular drugs i le glass ‘Sputum specimens should be collected in a sterile gl jarcontaining no preservatives because: ‘a. These substances dissolve the tubercle bacilli b. These substances are frequently inhibitory to growth of tubercle bacilli cc. These substances are injurious to the media used 4. Allof the above ‘Mycobacterium tuberculosis stains readily with: a. Gram’sstain b. Papanicolaou stain cc. Ziehl-Neelsen stain 4. Wayson’s stain concentrates of treated sputum specimens con- cacigg Af tuberculacie are "washed" in ord: ‘a. Remove the chemical used in pre-treatment to avoid killing M. tuberculosis by too long contact b. Remove contaminants and cellular debris ¢. Neutralize the acidity or alkalinity of the sediment Remove toxic pH indicators ; e. Prevent killing of animals by inoculation of a Vol. I, p. 89 a Vol. I, p. 86 a Vol. I, p. 82 b Vol. I, p. 83 b Vol. 1, p. 81 c Vol. 1, p- 30, 3 a Vol. 1, 9. 81 acid suspensions 164. 165. 166. 167. 168. 169, 170. m1, 172. 173. Exam Questions, Bacteriology & Allied Fields 251 ‘Any medium used for the isolation of M. tuberculosis ‘must contain: a. Whole eggs b. Potato flour ©. Asparagine a Milk ¢. Malachite green Glycerin in the medium for growing M. tuberculosis tends to cause colonies to remain regular. T, F ‘The common disadvantage of the direct smear and cultur methods in the diagnosis of tuberculosis is that they: ‘May be complicated by overgrowth of contaminant a. b. Fail to distinguish bovine from mammalian types ¢. Do not distinguish pathogenic from nonpathogenic ‘organisms Are very time-consuming fe. Are very expensive laboratory procedures One of the advantages of using Middlebrook's 7H10 ‘medium in tuberculosis work is more rapid developmen of colonies, T, F ‘Serpentine cord formation is best observed in Middle- brook 7H10 colonies using high power ocular, T, F Acid-fast organisms resist decolorization by acid- alcohol after being stained with basic fuchsin. T, F INH resistant human tubercle bacilli are often: a. Catalase-positive b. Catalase-negative Pathogenic tubercle bacilli are morphologically similar {to nonpathogenic species of Mycobacterium. T, F Before discarding a culture as negative for Mycobac- terium tuberculosis, it should be incubated and ‘examined every week for at least: a. 2weeks b, 1 month 2 months 4 months Malachite green is usually added to media for the cultivation of Mycobacterium tuberculosis in order to: a. Show a pH change © Vol. I, p. 93 F ‘Vol. I, p. 87 A Vol. 1, p. 81 1 Vol. I, p. 87 F Vol. I, p. 86 T Vol. 1, p. 31- 33 b Vol. I, p. 45 T Vol. I, p. 86 c Vol: I, p. 84 b. Confer a particular color to the colonies252 Exam Questions, Bacteriology & Allied Fields cc. Speed up growth of tubercle bacilli d_ Prevent growth of contaminating organisms 174, A strong acid or alkaline (such as 4% NaOH) solution ‘will not kill the majority of tubercle bacilli but is likely to destroy all other bacteria that may be present in a given clinical specimen. T, F 175. Indicate advantage of Kinyoun’s method over Ziehi-Neelsen method of acid-fast staining. 176. Which of the following are acid-fast? a, Hemophilus influenzae b. Mycobacterium tuberculosis ¢. Francisella tularensis Mycobacterium leprae 177, Tubercle bacilli may be present in gastric contents (or gastric washings) in cases of pulmonary tuberculosis. TF 178. Indicate a suitable medium for the cultivation of Mycobacterium tuberculosis: a. THIO b. Petragnani’s ¢. Dubos oleic acid 4. Lowenstein-Jensen fe. All of the above 179, _Allof the following may be spread by droplets except: a. Tuberculosis b. Pneumonia c. Typhus Whooping cough 180. A reagent often used for the digestion of sputum and other tenacious materials prior to culture is all of the following except: a. Antiformin b. NaOH ¢. Neavety-L-cysteine- NaOH d Caroid 181. Blood agar and other catalase-containing media should 182. Virulent strains of human tubercle bacilli are cord- forming. T, F a Vol. I, p. 30- 3 ft Vol. I, p. 82 former requires no heat Vol. I, p. 30, 31 bd Vol. I, p. 18, 85 rT Vol. I, p. 81 © Vol. I, p. 93 c Vol. I, p. 23 Vol. I, p. 82 T a Vol. I, p. 86 183, 184, Exam Questions, Bacteriology & Allied Fields 253 Human type strains of Mycobacterium tuberculosis are niacin-negative. T, F ‘The niacin test is used to distinguish human type strain of Mycobacterium tuberculosis from the non-human varieties. T, F F Vol. I, p. 89 T Vol. I, p. 89 185. 186. 187. 188. CORYNEBACTERIA, LISTERIA, ERYSIPELOTHRIX ‘The in vitro virulence test for C. diptheriae does not ‘a. Diphtheria antitoxin in optimal concentration b. Medium which facilitates the growth of C. iphtheriae ¢. Medium enriched with serum to facilitate diffusion of both toxin and antitoxin 4. Pure cultures of the strain to be tested €. Asstrain known to be capable of starch utilization In principle, all positive virulence tests for C. diphtheriae depend upon: ' a. The precipitation of diphtheria toxin by specific antitoxin in vitro b. Typical necrotic lesions of the skin of a guinea pig inoculated intracutaneously with a suspension of virulent organisms ©. The presence of cherry red adrenals in a guinea Big inoculated insapertoneally with C. diphieriae presence of diphtheria toxin in the test suspen- sion as determined by the Ramon flocculation test e. Evidence of the production of diphtheria toxin identified by its reaction with specific diphtheria antitoxin ‘When a single guinea pig is used for virulence tests in C. diphtheriae, the dose of antitoxin is administered: hours after the tests are injected After the "control" set of injections is made Intracutaneously 5 hours after the test injection Before any injections are made Subcutaneously 4-5 hours after the test set of injections is made opooe ‘The characteristic metachromatic granules in Corynebacterium diphtheriae are called: a. Rideal-Walker granules b. Babes-Emst granulates e Vol. I, p. 78 e Vol, p. 78 e Vol. I, p. 78 cc. Koch-Weeks granules254. Exam Questions, Bacteriology & Allied Fields 189, 190. 191. 192. 193. 194, 195. 196. d. Much's granules The final diagnosis of diphtheria is made from: a. Cultures b. Morphological characteristics c. Virulence test Serological typing C. diphtheriae may be distinguished from other members of genus by the fact that it: a, Ferments lactose and not dextrose b. Ferments dextrose and not saccharose c. Ferments saccharose and not dextrose d Ferments both dextrose and saccharose ‘There is no biochemical difference between C. diphtheriae and diphtheroids. T, F ‘Toxoid is used in diphtheria prophylaxis. T, F 7 bey ‘cally d “decolorized in Gram stain than 0 EEE armen tm «. More pleomorphic than diphtheoids 4. More frequently found in exudates from well persons than are diphtheroids It. essential that isolates suspected of being C. diphtheriae be subjeced to virulence testing before a final report is sent out. The Frobisher in vivo test is commonly used. T, F Corynebacterium diphtheriae may:. a. Be either toxigenic or non-toxigenic b. Hydrolyze urea and reduce nitrates ¢. Produce acid from sucrose only and be non-toxigenic : Be toxigenic, fail to reduce nitrates, and hydrolyze ‘urea A spinal fluid from a penton submited to he lb oratory. After culturing on s plaltes, {gram postive rod resembling diphtheroid i Observed from the small, gray, translucent, beta hemolytic colonies. ‘The organism most probably is: a. C.diptheriae b. Listeria monocytogenes Vol. I, p. 76 © Vol. I, p. 76 b Vol. I, p. 78 F Vol. 1, p. 78 T c Vol. 1, p. 77 F Vol. I, p. 78 a Vol. I, p. 76 197. 198. 199. 200. 201. 202. Exam Questions, Bacteriology & Allied Fields 25 . C.ulcerans 4d Enysipelothrix insidiosa C. diphtheriae are nonsporulating, Gram-positive pleo- morphic rods with prominent granules, aerobic, human parasites. T, F Reactions which are characteristic of Listeria ‘monocytogenes are: a. Catalase-positive; motile; V-P posit produce acid from mannitol b. Catalase-positive; non-motile; V-P positive; do not produce acid from mannitol ©. Catalase-negative; motile; V-P negative; do not produce acid from mannitol Catalase negative; motile; V-P positive; produce acid from mannitol ; do not Tumbling mobility in cultures at 18-20° will aid in differentiating Listeria from diphtheroids. T, F Vincent's angina infections may be differentiated from diphtheria infections by: a. Inoculating a swab onto Loeffler's medium and making a smear from the growth for organisms morphologically resembling Borrelia vincenti b. By observing the presence of Gram-positive, tightly coiled spirochetes in a direct smear ¢. By the observations of an increased number of ‘Gram-positive fusiform bacilli and polymorpho- nuclear leukocytes in a direct smear d. By the presence of diphtheroids and the absence of organisms morphologically resembling C. diphtheriae in a smear taken from a 24-hour Loefflers slant, inoculated with a throat swab ©. Observation of large numbers of Rorrelia vincenti and fusiform bacilli in a direct smear taken| before antibiotics are given "Diphtheroid baclli" are nonpathogenic to man and guinea pigs. T, F Listeria monocytogenes should be considered if a beta hemolytic colony on defibrinated sheep blood agar ‘demonstrates: a. Gun-grey colonies on tellurite-blood plates b. Tumbling motility in hanging drop preparation ¢. A brownish iridescence when viewed with obliquel transmitted light b Vol. I, p. 20 T Vol. I, p. 76 a Vol. I, p. 75 T Vol. 1, p. 75 e Vol. I, p. 140 T Vol. I, p. 140 b256 Exam Questions, Bacteriology & Allied Fields 203. 205. 206. 207. 208. 209. 210. 2u1. Transparent colonies Diphtheroid organisms may be pathogenic. T, F A mixed culture should be used to carry out an in vitro test of virulence for diphtheria bacilli. T, F Erysipeloid in man is due to infection wi a. Klebsiella pneumoniae b. Spirillum minus €. Bnysipelothrixrhusiopathiae Listeria monocytogenes Indicate the selective media suitable for the isolation of diphtheria: a. Loefflers blood serum b. Tinsdale's ©. Blood sugar d._ Cystine-tellurite-blood agar tis impossible to make a final diagnosis of diphtheria on the basis of stained direct smears alone. T, F Virulence tests are all that are necessary to confirm a presumptive laboratory diagnosis of diphtheria. T, F ‘A Gram-stained smear from a colony on an aerobic blood agar plate shows slender Gram-positive bacilli of variable length. These organisms are pleomorphic and barred, granular or club-shaped appearance. are. in palisades and cuniforms. The organism is likely to be: a. Haemophilus influence b. Corynebacterium diphtheriae ©. Bacillus subtilis Klebsiella pneumoniae €. None of these On tellurite medium the colonies of Corynebacterium diphtheriae are opaque, raised, convex, and: a. White b. Cream-colored ©. Green 4 Gun metal in color ‘The smallest amount of diphtheria toxin which, when injected subcutaneously, will kill a 250 gram guinea pig within four days is the: Vol. I, p. 75 T Vol. I, p. 18, 76 F Vol. I, . 78 c Vol. 1, p.20 bd Vol. I, p. 78 T Vol. I, p. 77 F Vol. I, p. 76 b Vol. I, p. 76 a Vol. I, p. 78 212. 213. 214. 215. 216. 217. 218. Exam Questions, Bacteriology & Allied Fields 257 MLD Ly dose Lo dose "dose ApoE ‘The smallest amount of diphtheria toxin which, when mixed with one unit of antitoxin and injected subeutan- ously into a 250 gram guinea pig, will pr. within four days isthe: Ml Produce death a Lpdose b. Ly dose : . Lydose a MDL To confirm a laboratory diagnosis of diphtheria itis essential to: a. Grow characteristic colonies in special media b. Find typical granular staining bacilli in smears ¢. Demonstrate toxin production 4d. Study biochemical behavior BRUCELL: PASTEURELLA, YERSINIA, FRANCISELLA Francisella tularens transmitted by: a. The fly, Chrysops b. The tick, Dermacentor ©. The rabbit louse d Allof the above essentially a parasite of rodents Specimens most generally submitted to lab for culture when suspecting tularemia include: a. Repeated blood cultures b. Sputum cc. Urine. d Allof these A selective medium for Francisella tularensis is: a. Blood glucose cystine agar . Cystine tellurite blood agar ¢. Desoxycholate citrate, lactose, sucrose agar Castanada’s medium ‘Tularemia can be transmitted to man from rabbits. T, F| F. wlarensis will not grow on nutrient agar. T, F a Vol. I, p. 95 b Vol. I, p. 95 © ‘Vol. I, p. 78 d Vol. I, p. 16, 139"? b Vol. I, p. 16 a Vol. I, p. 37 T Vol. I, p.16, 139 T Vol. I, p. 57258 Exam Questions, Bacteriology & Allied Fields Exam Questions, Bacteriology & Allied Fields 259 219. Tularemia is primarily a human infection. T, F F 228. The plague bacillus is technically known Vol. I, p. 139 a. Yersinia pestis b, Pasteurella multocida 220. A practical way to diagnose Francisella tularensis is to ¢. Francisella tularensis a hheat a saline suspension of organisms for 30 minutes at None of these Vol. I, p. 70 (60°C are then with this bacterial suspension set up agglutination tests, with Francisella tularensis antiserum. 229. Bubonic plague is caused by a: TF T a. Rickettsia b. Protozoan 221. Francisella tularensis will not grow on any medium — | T c. Virus d except cystine-glucose blood agar. T, F Vol. 1, p. 37 Bacterium . Vol. I, p. 70 222. Indicate suitable medium for the cultivation of 230. Species of pathogenic bacteria requiring an increased Francisella tularensis: ‘amount of CO> in the atmosphere of the culture are: a. Glucose-cystine agar a. Brucella abortus b. Glycerin-potato-blood agar b. Francisella tularensis: c. Petragnani a ©. Yersinia pestis a a EMB Vol. I, p. 37 Pasteurella multocida Vol. I, p. 71 223. Yersinia pests is a parasite of: 231, Brucella abortus is differentiated from the other a. Fleas Brucella species by: b. Rodents a. Its growth in thionine, and not in basic fuchsin c. Cattle and swine b b. Its growth in basic fuchsin, and not in thionine @ Bins Vol. I, p. 134 ¢. Its growth in both thionine ‘and basic fuchsin b Its growth in neither thionine nor basic fuchsin | Vol. I, p. 71 224, Cultural characteristics of Yersinia pestis are: a. Fermentation of carbohydrates 232. Brucella abortus grows best in: b. Non-hemolytic on blood agar a. The presence of 10% CO3 at 37°C ¢.. No liquefaction of Loeffler's blood serum medium } b. Castaneda's medium & Allo the above Vol. 1, p. 70 ¢. Both of these < Neither of these Vol. I, p. 71 225. Yersinia pestis when stained shows: a. Metachromatic granules 233. COpis required for the growth of: b. Bipolar staining a. Brucella melitensis . Gram-positive reaction b. Brucella suis d Allofthe above b ©. Brucella abortus ec d Allof the above Vol. 1, p. 71 226. Yersinia pestis produces a variety of diseases, including: a. Bubonic plague 234. In brucellosis, the specimen most often producing a b. Pneumonic plague positive culture is: ©. Septicemic plague d a. Blood d. Allof the above Vol. I, p. 134 b. Tissue c. CSF a 227. Non-motile, non-sporulating, pleomorphic a Urine Vol. I, p. 71 Gram-negative rods having bipolar staining are: a. Pseudomonas aeruginosa 235. Malta fever is spread to many by means of goat’s milk b. Yersinia pestis infected with: ©. Corynebacterium diphtheriae b a. Brucella abortus Brucella suis Vol. 1, p. 70 b. Brucella suis260 Exam Questions, Bacteriology & Allied Fields 236. 237. 238. 239. 240. 241. 242. 243. 244. 245. c. Brucella melitensis d Allof these Brucellergin is used: To prevent the spread of Brucella abortus in cows ‘As a skin test for brucellosis in man To differentiate the species of Brucella ‘As a most specific diagnostic aid in determining ‘brucellosis Brucella abortus causes undulant fever in man, T, F aoge Pasteurization will kill any Brucellae that may be present in the milk. T, F Brucellosis is primarily a human disease, T, F Which Brucella species require 10% CO2? a. Brucella abortus b. Brucella melitensis c. Brucella suis Brucella melitensis, Brucella abortus, and Brucella ‘suis may cause: a. Glanders b. Soft chancre c. Relapsing fever d Undulant fever ‘The genus Brucella is Gram-positive. T, F ‘Trypticase soy broth may be used for the isolation of Brucella, Pasteurella, and leptospira from the blood. T, F Indicate suitable medium for the cultivation of Brucella abortus: a. Sabouraud’s agar b. Loeffler blood serum c. Trypticase soy broth and agar d. Cystine-tellurite-blood agar HAEMOPHILUS, BORDETELLA ‘The phenomenon of “satellitism” is illustrated by: © Vol. I, p. 139 Vol 1.16 ol. I, p. 16, 1 T Vol. I, p. 71 T Vol. 1, p. 125 F Vol. I, p. 71 a Vol. 1. p. 71 d Vol. 1, p. 71 FE Vol. 1, p. 71 F Vol. I, p. 22 © Vol. 1, p. 71 246. 247. 248, 249. 250. 251. Exam Questions, Bacteriology & Allied Fields 261 a. Staphylococcus aureus b.. Haemophilus influenzae ©. Bacillus megatherium Yersinia pestis ‘The satelite phenomenon is an example of: a. Symbiosis b. Parasitism cc. Commensalism d Saprophytism ‘A Gram-negative cocco-bacillus isolated from CSP, blood, sputum, and urine which rarely grows on EMB, produces a hemolytic colony on BAP, and is often resistant to antibiotics is most likely: a. Bartonella bacilliformis b. Hemophilus influenzae ©. Acinetobacter spp. Neisseria meningitidis An organism which must be isolated from a medium containing egg-yolk which produces a venereal disease in which aspirates of the inguinal nodes are found to contain Gram-negative bacilli with a distinct capsule is most likely: aT. pallidum b. Haemophilus ducreyi ©. Chlamydia trachomatis Neisseria gonorrheae Which of the following species of Haemophilus do not require both the X and V factors? . a. H. influenzae b. Koch-Weeks bacillus ©. Hi hemolyticus None of the above73 Soft chanere is associated with what organism: H. aphrophilus b. HL aegypticus ¢. H. Ducreyi d Hinfluenzae Haemophilus ducreyi has the following characteristics: a. Is the cause of a venereal disease b. Is an intracellular organism resembling a cigarette package 7 ¢. Requires freshly drawn rabbit blood in the culture b Vol. I, p. 137 Vol. I, p. 137 © Vol. Ip. 60 c Vol. I, p. 97 Vol. I, p. 72, Vol. I, p. 72262. Exam Questions, Bacteriology & Allied Fields Exam Questions, Bacteriology & Allied Fields 263 All of the abo 4 Allof ve 260. Match the following: 1. Hemophilus of a. Causes pseudomembranous 252. Haemophilus i res media containing: ee e Morax-Axenfeld conjunctivitis SV factor 2. Corynebacterium b. Causes infectious conjuncti- | 1-c,2-2,3-b c. Both of the above le diphtheriae vitis ("pink eye") Voi. I, p. 20. & Neither ofthe above Vol. 1, p. 72 3. Haemophilus of c. Causes blepharoconjuncti-. | 71 Koch-Weeks vitis 254, ‘The satellite pheiomenon works on the basis that: : aoe cy acptococeus supplies the X factor necessary 261. ‘The genus Haemophilus is gram-negative. T, F T ee poetic! He Gece EAP. tie Vol. I, p. 16 b. Staphylococcus supplies the coenzyme BAP, . Q X factor necessary for the growth of Haemophilus 262. Haemophilus influenzae, as well as other hemo- c. Staphylococcus supplies the hemin factor, globinophils, will grow well on: ‘Chocolate the V factor a. Chocolate agar with yeast extract 4. Staphylococcus will provide all the necessary. b. Lowenstein-fensen medium c nutrients for the growth of Hemophilus on any |b cc. Rabbit blood agar with yeast extract Vol. I, p. 16, type of media Vol. I, p. 137 d_Desoxycholate agar 7 255. Factor V, a heat-labile substance used in culturing certain fastidious organisms, is found in: 263. The V factor (coenzyme I or Il) is: a a. Serum fo tee ae Vol. I, p. 16, B Yeast b . Heat stablel7 7 c, Hemoglobin Vol. 1, p- 16, 24 The Pfeiffer’ bacillus is technically called: d Allof these 7 Lp te le ly called: 256. Beta streptococci are small, white, rather opaque and b. Haemophilus influenzae ‘usually quite firm colonies with no zones of hemolysis.) F ¢. Mycobacterium leprae b oA Vol. fp. 17 @ Hemophilus ducreyi Vol. I, p. 17 257. Hemophilus influenzae may caiise respiratory tract 8 hoe eee rere infections as well as: a. Ktellurite agar a. Enteric fever es b, Food poisoning scitic fluid agar a cc. Meningit c : 4. Potato-blood-glycerol agar Vol. I, p. 37 4. Subacute infectious conjunctivitis Vol. 1, p- 1 oe ee a. Bordet-Gengou bacillus 258. Hemophilus hemolyticus is pathogenic. T, F E aa fol. Lp. 7 ©. Morgan's bacillus A 259, Softchanere is due to infection with: id Kich, Weeks bacillus Vol. 1, p. 37 a. Chlamydia trachomatis B. Nessera sca, 261. Whoopi ing cough is caused by: : ©. Haemophilus ducreyi © . Moraxella lacunata Vol. I, p. 17 © Sonata aay d. Koch-Weeks bacillus Vol. p. 70 268. Bordet-Gengou agar base with fresh blood is used for the isolation of:264 Exam Questions, Bacteriology & Allied Fields Exam Questions, Bacteriology & Allied Fields 2 a. Haemophilus influenzae b. Corynebacterium diphtheriae 277. Tetanus is caused by: c. Bordetella pertussis ¢ a. Clostridium perfringens d._Listeria monocytogenes Vol. I, p. 37 Clostridium tetani - ¢. Clostridium histolyticum b 269. Morphologically, Bordetella pertussis is very similar to Clostridium novyi Pa ‘a. Proteus morgani fol. I, p. 18 >. Pseudomonas aeruginosa 278. Gas gangrene may be caused by: c. Klebsiella pneumoniae a a. Clostridium septicum Haemophilus influenzae Vol. I, p. 70 b. Clostridium novyi c, Clostridium perfringens 270. On glycerin-potato-blood agar the colonies of Clostridium histolyticum « Bordetella pertussis are small and ‘resemble: €. All of these a. Dew drops f. None of these '. Maple syrup droplets 279. Food poisoning (botul c. Mercury droplets ¢ ). Food poisoning (botulism) is caused by a toxin None of the above Vol. I, p. 70 ioe prein ‘improperly canned or preserved 271. Indicate suitable medium for the cultivation of a. Clostridium tetani Bordetella pertussis: b.- Clostridium botulinum a. Loeffler’s serum 7 cc. Clostridium perfringens lb b. Glycerin-potato-blood agar Clostridium histolyticum ve ¢. Bordet-Gengou agar bed : ol. 1, p. 18 cae Vol. 1 p. 16 280. Indicate suitable media for the cultivation of m = reforming anaerobes (Clostridium group), anaerobic 272. Glycerin-potato-blood agar is also called: streptococci, and Bacteroides: a. Bordet-Gengou medium a. Petragnani b. Petragnani's medium b. PRAS-Brucella blood agar c. Loeffler’s medium a c. Lowenstein-Jensen d Sabouraud's medium Vol. I, p. 16 d_ Bordet-Gengou Vor Lp. 18 1p. 273. Bordetella pertussis requires factors X and V for growth) F 281. The species of Clostridium most frequently TF Vol. I, p. 16 ieolated fiom clinical materials through anaerobic ‘CLOSTRIDIA a. Clostridium botulinum rulating rods, T o Gtourdiun port 274. —_ Clostridia are gram-positive spor lating 3, Some are c lostridium ens motile, anaerobic, some are pathogenic. T, F Vol. 1, p- 18 © Costin neers aad 282. A member of the genus Clostridium which will not, 275. CL tetas readily distinguishable fom other nber members of the Clostridium genus by its: as a rule, form spores unl ts 2. Production of indol medium is: eros is asia foe b. Distinctive sporulating form a. Clostrdium botulinum c. Presence in the intestine of horses b b. Clostridium tetani d_ Allof the above Vol. 1, p. 18 ©. Clostridium perfringens . Clostridium histolyticum ve 276. Clostridium perfringens is a normal inhabitant (con- ol. I, p. 18 stantly present in small numbers) of the human T 283. In solid media and in thioglycollate broth, Vol. I, p. 18 Clostridium tetani produces spores which are: intestine. T, F a. Round and terminal266 Exam Questions, Bacteriology & Allied Fields b. Oval and eccentric | Oval and subterminal Usually absent id media and in thioglycollate broth, ™ Gierridium perfringens produces spores which are: a. Round and terminal . Oval and eccentric | Oval and subterminal Usually absent a Vol. I, p. 18 4 Vol. I, p. 18 285. To confirm a laboratory diagnosis of tetanus it is $°°" Grow characteristic colonies through anaerobic 'b. Find typical sporulating baci >. Demonstrate toxin production ._ Study biochemical behavior © Vol. I, p. 13 36. The members of the genus Clostridium are: : a. Obligate aerobic spore-formers . Obligate anaerobic spore-formers Facultative anaerobic spore-formers 287. Clostridium perfringens was formerly known as: a. Bacillus oedematiens b Vol. I, p. 18 b. Bacillus tetani ¢. Bacterium welchii Bacillus histolyticus DACTEROIES a Bacteroides is a non-sporogenous, anaerol vic me negative rod tending to produce filaments. T.F 289. Bacteroides has been jsolated from the following cases: a. Puerperal infections ‘b. Urinary tract infections c \dicit 4. Food poisoning < | Vol. 1, p- 18 r Vol. 1, p- 20 ac Vol. 1, p. 20 Bacteroides: “i il well as F 290. Bacteroides spies wil gow aroiealY SVENS Voy 1, p. 20 291. Choose onganism which is anaerobic, gram-negative, ‘and culturally fastidious: c Vol. I, p. 20 e i 4. Micrococcus luteus ‘Exam Questions, Bacteriology & Allied Fields 267 MORAXELLA 292. Moraxella lacunata is a Gram-negative coccobacillus. | T TF Vol. 1, p. 20 293. Organisms belonging to the Moraxella genus are: a. Gram-negative b. Anaerobic ¢. Motile rods a d_Non-pathogenic to man . Vol. I, p. 20 294, "Glanders” is due to infection with: a. Moraxella lacunata b. Malleomyces mallei Streptococcus pyogenes Bacillus anthracis 6 Vol. 1, p. 132 295. Theetiologic agent of subacute infectious, conjunctivitis is: a. Hemophilus ducreyi . Moraxella lacunata ¢. Clostridium perfringens b 4d Streptobacillus moniliformis Vol. I, p. 20 BACILLUS 296. The only pathogenic Bacillus is Bacillus anthraxis. T, H] T Vol. I, p. 20 297. The Ascoli testis used for the diagn a. Malleomyces mall b. Bacillus anthracis c. Yersinia pestis Ascaris lumbricoides of: b Vol. I, p. 20 298. A test used for the diagnosis of anthrax in animals dead Of the disease, or to detect contamination of hides by B. anthracis, is known as: a. Schick test b. Mallein test ¢. Ascoli precipitation test c Ramon flocculation test Vol. I, p. 20 FUNGI 299. Mycelia are ‘a. Sexual spores268 Exam Questions, Bacteriology & Allied Exam Questions, Bacteriology & Allied Fields 26: b. Asexual spores ¢. Epidermophyton floccosum -€ Mass or mat of hyphae c Microsporum audouinii Sa ‘Roots and stems of fungi Vol. 1, p. 105 rol. 1, p. 107 300. Hyph be tate or septate which hel T ar racquette one with peecoreinast mlcroconkd |. Hyphae may be nonseptate or septate which helps ra fm, ood bypas snd ota’ bod determine the classification of the fungi. T, F Vol. I, p. 105 Ee tmonty tcely to be: and nodular bodies ‘a. Trichophyton spp. 301. Chlamydospores are structures seen in: b. Microsporum spp. &. Miresporan i Rhicope pp 4 Microsporum 5 Spp. Mucor 4 7 ” vou ep. 107 Candida albicans Vol. I, p. 105 . “Sulfur granules" (characteristic yellow in purlent materia) are associa wid 302. A fungi in which the mycelium lacks septation is: &. Nocardia spp. a, Coccidioides 8. Blasiomyeasts spp Ee Claim, f >’ ctinomyces israelti Vol. I, p. 106 = tt ‘Vol. I, p. 110 3 organism is partially acid- is 303. A fungi which is responsible for dermatomycoses is: pigmented: direct aeela han) a. Mucor Se Actinomyces sop b. Rhizopus Rhizopus spp. -€. Microsporum c -e Nocardia spp. fc d Coccidioides Vol. I, p. 106 d Chromoblastomycosis Vol. 1, p. 110 304. The collection of specimens for dermato- 310. The geographic distribution of North American phytes from the skin includes: blastomycosis is 4. Area cleansed with soap and swabbed with cotton a. Central regions of California applicator b. Northeastern areas 'b. Area cleansed with 70% alcohol and swabbed with _ : Mississippi Valley and the Southeastern areas © cotton applicator @ Adjacent to the Northen border of the U. S. Vol. I, p. 11 cc, Scrape tissue with scalpel from center of infected : Lp. lt area 311. The identification of Blastomyces dermatitidis includes: -& Area cleansed with 70% alcohol and scraped a a. The yeast phase at room temperature to the - from the border with scalpel Vol. 1, p. 106 mycelial phase at 37°C ty: The myedlial phase at room temperature tothe 305, The conect examination of specimens for derma yeast phase at 379C phytes from direct smears includes: ¢. The organism only grows on enriched 1. Gram stain material and examin: for hyphae medium id [ Bloc 'b. Material placed in isotonic saline and examine for Examination of hyphae for barrel-shaped b hyphae arthrospores cc. Material is placed in 10% HSOq and examine Val Tp. 111 for hyphae Candida albicans may be differentiated from other d_ Material is placed in 10% NaOH and examine for | species of Candida by: fom hyphae Vol. I, p. 106 ‘a. Barrel-shaped arthropores b. Basidiospores 306, Epidemic ringworm of the scalp in children is due to: © Ascospores a. Trichophyton mentagrophytes a Chlamydospores vai Leal b. Microsporum gypseum cep. Au