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    karuna micro
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    This document provides a worksheet for real-time PCR to detect COVID-19. It lists the components needed for the reaction mix, including probes labeled with different fluorescent dyes to detect the ORF1ab gene, N gene, and internal control. Volumes are given for reactions using 5 or 10 microliters of sample. Space is included to record the template added, sample details, and results.

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    WORK SHEET

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    This document provides a worksheet for real-time PCR to detect COVID-19. It lists the components needed for the reaction mix, including probes labeled with different fluorescent dyes to detect the ORF1ab gene, N gene, and internal control. Volumes are given for reactions using 5 or 10 microliters of sample. Space is included to record the template added, sample details, and results.

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    © All Rights Reserved
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    3 views2 pages

    Work Sheet

    Uploaded by

    karuna micro
    This document provides a worksheet for real-time PCR to detect COVID-19. It lists the components needed for the reaction mix, including probes labeled with different fluorescent dyes to detect the ORF1ab gene, N gene, and internal control. Volumes are given for reactions using 5 or 10 microliters of sample. Space is included to record the template added, sample details, and results.

    Copyright:

    © All Rights Reserved
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    Worksheet for Real-Time PCR for detection of COVID-19 using Meril COVID-19 One Step RT-PCR Kit

    DATE:
    ReactionmixPreparation: Total Volume: 20µl (for one reaction)
    Corona ORF1ab Gene Probe:Reporter- FAM, Corona N Gene Probe: Reporter- HEX
    IC Probe: Reporter-ROX
    1 × Volume Required Quantity N= Done
    For 5 μL Sample For 10 μL Sample

    Resuspended master mix 9 μL 9 μL


    ORF1ab/N/ICON Primer & 1 μL 1 μL
    probe (FAM/HEX/ROX)
    RNase-free water 5 μL -
    Total volume 15 μL 10 μL
    Addition of Template

    Template (RNA) 10 μL
    Sample details (sample ID):

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    Worksheet for Real-Time PCR for detection of COVID-19 using Meril COVID-19 One Step RT-PCR Kit

    Results for Covid 19

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