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Growth Promotion Test
Growth Promotion Test
1.0 PURPOSE :
To give procedure for growth promotion test.
2.0 SCOPE :
This is applicable to check the nutritive and selective properties of media used for all microbiological
analysis.
3.0 RESPONSIBILITY :
Microbiologist.
4.0 FREQUENCY :
Each lot of media prepared or must be performed atleast on each lot of dehydrated media.
5.0 PROCEDURE :
Growth promotion test is done to test each lot of the medium for its growth promoting qualities. Media
plates or broth should be inoculated with relevant organisms of each strain.
The test media are satisfactory, if clear evidence of the growth appears in all inoculated media within the
given incubation period.
If freshly prepared media are not used with in 2 days store them in dark at room temperature.
5.1. MEDIA PREPARATION :
Media preparation and composition refer the SOP No. QC/MB/
5.1.1 Media controls :
Each batch of medium sterilized must be checked to ensure growth of the relevant microorganism(s) and
to ensure that the blank, plates are not contaminated.
5.2 TEST MICROORGANISMS