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References
1- Getahun H, Matteelli A, Abubakar I. et al.
Management of latent mycobacterium
tuberculosis infection: WHO guidelines for low
tuberculosis burden countries. Eur Res J. 2015;
46: 1563-76.
2- Selgelid MJ. Ethics, tuberculosis and
globalization.Public Health Ethics.2008; 1: 10-20.
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3- Shorten RJ. The molecular epidemiology of
Mycobacterium tuberculosis in north London:
UCL. 2011.
4- Al-Hajoj SA, Zozio T, Al-Rabiah F, et al.
First insight into the population structure
of mycobacterium tuberculosis in Saudi Arabia.
J Clin Microbiol. 2007; 45: 2467-73.
5- Alexander KA, Laver PN, Michel AL, et al.
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Novel mycobacterium tuberculosis complex
pathogen, M. mungi. Emer Infec Dis. 2010; 16:
1296-9.
6- Salek S, Emami H, Masjedi MR, Velayati AA.
Epidemiologic status of tuberculosis in Golestan
province. Tanaffos. 2008; 7: 63-8.
7- Alcaide F, Coll P. Advances in rapid diagnosis
of tuberculosis disease and anti-tuberculous drug
resistance. Enfermedades Infecciosasy Microbiol
Clín. 2011; 29: 34-40.
8- Daniel TM. The history of tuberculosis. Resp
Medic. 2006; 100: 1862-70.
9- Nastasee S. Zoonotic tuberculosis in Africa.
Med J Therapeut Africa. 2008; 1: 53-8.
10- Ayele W, Neill S, Zinsstag J, Weiss M, Pavlik
I. Bovine tuberculosis: an old disease but a new
threat to Africa. Int J Tuberc and Lung Dis. 2004;
ve
8: 924-37.
11- Lantos Á, Niemann S, Mezõsi L, et al.
Pulmonary tuberculosis due to mycobacterium
hi
bovis in Captive Siberian Tiger. Emer Infec Dis.
2003; 9: 1462-69.
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12- Stewart GR, Newton SM, Wilkinson KA,
et al. The stress-responsive chaperone a-crystallin
A
2 is required for pathogenesis of mycobacterium
tuberculosis. Mole Microbiol. 2005; 55: 1127-37.
13- Maguire H, Dale J, McHugh T, et al.
Molecular epidemiology of tuberculosis
London 1995–7 showing low rate of active
transmission. Thorax. 2002; 57: 617-22.
14- Mostowy S, Cousins D, Brinkman J, Aranaz
A, Behr MA. Genomic deletions suggest a
ÎÐÖÒŶƴƠſřƹƲưƸŝÎÍÔƽƵŹŚưƃ
 ÏÑƽƵŹ
phylogeny for the mycobacterium tuberculosis
complex. J Infec Dis. 2002; 186: 74-80.
15- Medeiros L, Marassi C, Duarte R, da Silva M,
Lilenbaum W. Comparison of decontamination
methods for primary isolation of mycobacterium
bovis in paucibacillary bovine tissues. Letters in
App Microb. 2012; 54: 182-6.
16- Warren R, Gey van Pittius N, Barnard M, et
al. Differentiation of mycobacterium tuberculosis
complex by PCR amplification of genomic
D
regions of difference. Int J Tuberc and Lung Dis.
2006; 10: 818-22.
SI
17- Goude R, Parish T. Electroporation of
mycobacteria. mycobacteria Protocols: Springer;
2009; 203-15.
of
18- Pym AS, Brodin P, Brosch R, Huerre M, Cole
ST. Loss of RD1 contributed to the attenuation of
the live tuberculosis vaccines Mycobacterium
bovis BCG and Mycobacterium microti. Mole
Microbiol. 2002; 46: 709-17.
19- Monajemzadeh M, Shahsiah R, Zarei A, et al.
Frequency of bacille calmette-guerin (bcg) and
mycobacterium tuberculosis in tissue biopsy
specimens of children vaccinated with bcg.
American J Clin Pathol. 2010; 133: 102-6.
20- Takahashi M. Molecular epidemiology of
Mycobacterium tuberculosis using by RFLP
in analysis between genomic DNA--its
accomplishment and practice. Tuberculosis. 2003;
78: 641-51.
21- Harris NB PJ, Bravo D, Osorio R, et al.
Recovery of mycobacterium bovis from soft fresh
 ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ
 
ƶƬŬƯ
www.SID.ir
 ƱŚŤſřƲǀƫƺƬƀƯŻř žƿƺŝƭƺƿźŤĩŚŝƺĪƿŚƯƽŻŚſřŶū 82

cheese originating in Mexico. App Env microbiol.
2007; 73: 1025-8.
22- K OR. Teasing out mycobacterium bovis' role
in the tuberculosis crisis. J Am Vet Assoc. 2005;
227: 871-78.
23- Müller B, Dürr S, Alonso S, et al. Zoonotic
Mycobacterium bovis-induced tuberculosis in
humans. Emer Infec Dis. 2013; 19: 899-908.
24- De la Rua-Domenech R.
Mycobacterium bovis infection in the United
29- Shojaei H, Rahimi-Hajiabadi H, Heidarieh P,
et al. Molecular microbiological investigation of
post-vaccination bacille Calmette-Guérin
infection in Iranian patients. Int J Tuber and Lung
Dis. 2011; 15: 1497-504.
30- Velayati A, Farnia P, Boloorsaze M, et al.
mycobacterium bovis infection in children in the
same family: transmission through inhalation.
Human Monaldi Archives for Chest Dis. 2007; 67: 169.
31- Tadayon K, Mosavari N, Feizabadi MM. An

D
Kingdom: incidence, risks, control measures and epidemiological perspective on bovine
review of the zoonotic aspects of bovine tuberculosis spotlighting facts and dilemmas in

SI
tuberculosis. Tuberculosis. 2006; 86: 77-109. Iran, a historically zebu-dominant farming
25- Akhavan R, Meshkat Z, Khajekaramadini M, country. Iranian J microbiol. 2013; 5: 1-13.
Meshkat M. Eight-year study of mycobacterium 32- Yaghoubi S, Mosavari N, Moradi BS, et al.
of
tuberculosis in Mashhad, northeast of Iran. Molecular typing of Mycobacterium tuberculosis
Iranian J Pathol. 2013; 8: 73-80. strains isolated from patients in Markazi Province.
ve

26- Hashemi A, Shojaei H, Heidarieh P, Aslani Iran South Med J. 2014; 17: 602-611
MM, Naser AD. Genetic diversity of Iranian 33- Soleimanpour S MN, Asl DH, Tadayon K, et
clinical isolates of Mycobacterium tuberculosis. al. Zoonotic tuberculosis caused by
hi

New Microbio. 2012; 35: 61-5. Mycobacterium bovis, central province, Iran. J
27- Shojaei H, Heidarieh P, Hashemi A, Feizabadi Lung Pulm Res. 2015; 2: 54-7.
rc

MM, Naser AD. Species identification of 34- Mozafari M, Farnia P, Afraei M,

neglected nontuberculous mycobacteria in a Derakhshani-Nezhad Z, Masjedi MR, Velayati
A

developing country. Japanese J Infec Dis. 2011; AA. Molecular diversity of Mycobacterium
64: 265-71. tuberculosis strains indifferent provinces of Iran.
28- Tabarsi P, Baghaei P, Farnia P, et al. Iranian J of microbiol. 2013; 5: 366-73.
Nontuberculous mycobacteria among patients 35- Mosavari N, Feizabadi MM, Jamshidian M,
who are suspected for multidrug-resistant et al. Molecular genotyping and epidemiology of
tuberculosis-need for earlier identification of Mycobacterium bovis strains obtained from cattle
nontuberculosis mycobacteria. The American J in Iran. Vet Microbiol. 2011; 151: 148-52.
Med Sci. 2009; 337: 182-4.

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83 ƱřŹŚƨưƷƹŹƺĜƳŚƤƷŵƽŶƸƯ

Isolation of Mycobacterium Bovis from Tuberculosis Patients in Zanjan Province (2013)

Dehghanpour M1, Baghcheh Saraee H1, Mosavari N2, Mazloom Zadeh S3

1
Dept. of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2
Razi Vaccine and Serum Research Institute, Reference Laboratony for Bovine Tuberclusis, Kraj, Iran
3
Zanjan Social Determinants of Health Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

Corresponding Author: Dehghanpour M, Dept. of Microbiology, Faculty of Medicine, Zanjan University of Medical
Sciences, Zanjan, Iran
E-mail: m.dehghanpour1983@yahoo.com
Received: 30 Sep 2015 Accepted: 10 Apr 2016

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Background and Objective: Tuberculosis (TB) is amongst the common infectious diseases in the world so

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that the World Health Organization (WHO) has declared it as a global emergency as well as an extremely
contagious disease. Although the cause of the disease and its therapy are well known, TB is considered as a
global dilemma. The present study was set to isolate and identify Mycobacterium Tuberculosis Complex by
of
PCR-RD typing from TB patients in Zanjan province and to verify cases of zoonotic tuberculosis.
Materials and Methods: In this study, 27 positive samples were collected from center of pulmonary diseases
and tuberculosis in Zanjan Province during 2013 and were grown on Lowenstein–Jensen medium (LJP and
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LJG). DNA was extracted from the bacterial isolates by Van Solengen method and at genus level
identification was done by PCR-16SrRNA. To confirm the mycobacterium tuberculosis complex, PCR-
hi

IS6110 was used. Identification of species was performed by PCR-RD typing (RD1, RD4, RD9 and RD12).
Results: A total of 27 isolates were obtained which were identified as genus Mycobacterium belonging to
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Mycobacterium tuberculosis complex. Twenty five of the isolates were Mycobacterium tuberculosis while 2
isolates were identified as M.bovis.
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Conclusion: The results of the study indicates that M.bovis species is present among the human TB cases in
Zanjan province, which indicates the zoonotic potential of the species and the possible transmission of the
species from animals to humans. These results might be alarming owing to the spread of the bovine strains
among the human subjects. Thus, it is essential that during research on suspected human TB cases, the
zoonotic species capable of transmission from animal to human be considered.

Keywords: Mycobacterium tuberculosis complex, Mycobacterium bovis, Mycobacterium tuberculosis,

PCR-RD typing

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