CULTURE MEDIA ial media are used for cultivation of bacteria fren clinical sainples. 6 like carbohydrates, proteins, electrolytes && water which pre vi nuitrle esseritin! fir the growth & multiplication of bacteria. CLASSIFICATION (Culture media can he classified in two ways! 1) Based on the consistency. 08 .iquid + Semi solid media (01.2 ~ + Solid media (2%) 2) Based of their use, ms - Simple media eg. Peplone water, Nutrient broth, Nutrient ager- Enriched media eg. Blood agar, Chocolate agar, [,oefiler’s serum slope, Dorset egg medina, Milk agar, Glucose broth, Brain heart Infusion broth, Enrichment medin eg. Selenite F broth, Tetrathionute bros, Alkuline peplone water. Bile broth, Selective media eg, Potassium teDurite agar, Wilson & Blair medium, Lowenstein Jensen mediutti. TIiesulphate citrate Bile sucrose agar (TCI Sulmonelia Shigelli agar. Deoxy cholate citrate agar, Differential media eg. MacConkey's azar, Xylose Lywine Decarboxs lase medium (XLD), Eosin Melliyfeng Blue agar. Transport meillu eg. Stuart's transport medium, Amie's trunsport medium, Cary Blair medium, Venkalneaman Ramakrishnan medium. Binchetnieal media eg. Triple sugor iron agnr, Simman’s eitrate medium, Christensen’s Urcase medium, Sugar fermentation medium, Methy] red — Voges Provkauer broth, Anaerobic media — Robertson's Cooked ineal medium, Thioglycolline broth. @ scanned with OKEN ScannerSIMMONS CITRATE y TUM NEGATIVE POSITIVE PRINCIPLE: This medium determines the ability of an organism to utilize citrate as sole source of carbon. KEY INGREDIENTS: Sodium citrate - Source of carbon. Ammonium dihydrogen phosphate- source of nitrogen Bromothymol blue - Indicator. MECHANISM: i Sodium citrate enzymes Alkaline end [+ P=] Ammonium dihydrogen phosphate |- ———————>_- products "romothymol blue at pH 6.9 : Bromothymol blue Green colour Blue colour RESULT: Interpret after incubation for 24 — 48hours at 35 -37°C Positive test: Growth with or without intense blue colour. eg: Klebsiella pneumoniae, Citrobacter species. Negative test: No growth and no change in colour, eg: E. coli, Shigella species, t @ scanned with OKEN Scanner‘sayaads vjjauowpog 109 viyaqcoyosy ‘80 eBuvyp snoj09 oN, 3380) OANLBON, sioonpoud osvain pidvy ~ sapoads snajoug apjuounaud oyyaisqary :80 E “anoj00 pas uid asuoquy #950) oantsog D pLE - SEI” SIUGy -p2 J0J UOREAnoUT JOYE youdiOUT -L INSTA anojoo pau ysPyutd anojoo Jgnq 0} MOTTA, ¥°8 -Hd qe pos jousug - 8'9-Hd ae pas jouaqa Gee 1 wmoune <——————- ia aseaan SWSINVHOGW OWwOIpUT — pax [ousyd: vain %0p ‘SINSIGHESNE TN SIALLISOd —“GALLVDIN Uvov vaun saraswals qHO VIGHW TVOUNAHDOIL @ scanned with OKEN ScannerANAEROBIC MEDIA ROBERTSON’S COOKED MEAT MEDIUM (CM) TYPE: Anaerobic medium. KKEY INGREDIENT: Minced meat, Peptone. STERILIZATION: Autoclaving [Fresh medium preferred] PRINCIPLE: Minced meat provides amino acids, nutrients and glutathione, a reducing, substance promotes the growth of anaerobes. USES: 1) Cultivation of facultative anaerobes, obligate anaerobes especially pathogenic Clostridia. 2) To determine proteolytic (blackening of meat and foul odour) and saccharolytic (pink color of the meat and sour odour) activity of bacteria. 3) Maintenance of clostridia. 4) Recovery medium for spores. @ scanned with OKEN Scanner@ scanned with OKEN Scanner@ scanned with OKEN Scanner