Jose B.

Cardenas Memorial High School- Uptown Campus

Science, Technology, Engineering and Mathematics Strand

APPROVAL SHEET

This Research entitled Lice Terminator: Madre De Cacao (Gliciridia Sepium), Guava

Leaves (Psidii Guajavae Folium) and Aloe Vera (Aloe Barbadensis Miller) Extracts

prepared and submitted by:

Insipedo, Crizel Ann M.

Palmares, Carla C.

Pata, Vicente Luis M.

In partial fulfillment of the requirements for the Academic Track, Science, Technology,

Engineering and Mathematics Strand is hereby approved.

______________________________ ______________________________

MARY CARMEL J. GAGA-A FLAVIANO T. CAWAS

Research Adviser Research Assistant Adviser

____________________________

REYNALDO C. NABU-AB JR.

TIC, JBCMHS-UPTOWN CAMPUS

Lice Terminator: Madre De Cacao (Gliciridia Sepium), Guava Leaves (Psidii Guajavae Folium)
and Aloe Vera (Aloe Barbadensis Miller) Extracts

A Research Paper

Presented to

Jose B. Cardenas Memorial High School-Uptown Campus

In Partial Fulfillment

of the Requirements

in Practical Research II

by

Insipedo, Crizel Ann M.

Palmares, Carla C.

Pata, Vicente Luis M.

ACKNOWLEDGEMENT
 TABLE OF CONTENTS

Page/s

INTRODUCTION ……………………………………………………………………...

Statement of the Problem ………………………………………………………

Hypothesis ……………………………………………………………………...

Scope and Delimitations ……………………………………………………….

Significance of the study ………………………………………………………

Definitions of Terms …………………………………………………………...

Review of Related Literature ………………………………………………….

METHODOLOGY ………………………………………………………………………… 23

Research design …………………………………………………………………….

Subjects of the study ……………………………………………………………….

Treatments

Media

Control

Data Gathering Procedure …………………………………………………………

Statistical Analysis ………………………………………………………………...

RESULTS AND DISCUSSION …………………………………………………………...

CONCLUSION ……………………………………………………………………………

RECOMMENDATION ……………………………………………………………………

REFERENCES …………………………………………………………………………….

APPENDICES ……………………………………………………………………………..
 INTRODUCTION

Background of the Study

According to the Centers for Disease Control and Prevention (CDC,2019), the pubic

region of people can also get lice, which are parasitic insects that live on people's head and

bodies. Human blood is the only food source for human lice. The types of lice found on various

parts of the body vary. The three kinds of lice that reside on people are: head lice (pediculus

humanus), body lice (pediculus humanus corporis) and pubic lice (pthirus pubic). Lice bites can

make a person's scalp itchy and irritated, and scratching that can lead to infection.

Moreover, we are thinking of something that is produced by nature, with the help of

Gliciridia sepium (madre de cacao) extract, Psidii guajavae folium (guava leaves) extract and

Aloe barbadensis miller (aloe vera) extract, we can possibly create a substance that could

possibly terminate a lice. Madre de cacao is a plant that contains pesticidal properties that we

believe could kill or terminate lice (pediculus humanus). Also, it contains anti-microbial, anti-

scabies, insecticidal and nematocidal that that can testify our hypothesis. Madre de cacao is a

folk remedy for alopecia, boils, bruises, burns, colds, cough, debility, eruptions, erysipelas, fever,

fractures, gangrene, headache, itch, prickly heat, rheumatism, skin tumours, ulcers, urticaria and

wounds. With the additional extracts of guava leaves and aloe vera extracts, we will be able to

produce a non-toxic substance when applied to human skin. Guava leaves contains an

antibacterial and anti-inflammatory properties while the aloe Vera contains extracts that is used

as moisturizer and safe for human skin.

This study is looking forward to formulate and invent a resourceful product that could alter the

infestation of lice. By extracting the substances of aforementioned plants, we can undergo a

series of procedure that can support the possible efficiency of our research concept.
Statement of the Problem

The main problem of this study was to create a lice terminator using the extracts of Madre de

Cacao, Guava leaves and Aloe Vera.

Specifically, this study aimed to answer the following questions:

1.How effective are Gliricidia sepium extract, Psidii guajavae folium extract and Aloe

barbadensis miller extract to the human lice?

2.How will the lice reacts to the plant extract?

Hypothesis

In the view of specific objectives, following hypothesis were advanced

• There is no certain chance that the plant extracts could kill a louse.

Scope and Delimitations

The goal of this research is to create a solution extracted from madre de cacao, guava leaves and

aloe vera that will kill hair lice (pediculus humanus capitis). The Madre de cacao leaves, guava

leaves and aloe vera will be squeezed to extract their plant juices. The following procedure will

be conducted and carried out at the Jose B. Cardenas Memorial High School-Uptown Campus

Senior High School Department Science Laboratory with the assistance of our quantitative

research teacher. Three treatments will be many treatments were used to replicate the lice

terminator. Treatment A (34% Madre de Cacao extract, 33% guava leaves extract and 33% Aloe

Vera extract), treatment B (50% Madre de Cacao leaves extract, 25% Guava leaves extract and
25% Aloe Vera extract) and Treatment C (75% Madre de Cacao leaves extract, 15% Guava

leaves extract and 10% Aloe Vera extract). The writing of the research paper, data collection and

experimentation was done from February to June 2023.

Significance of the study

The researchers believe that this study will not only yield data that will be helpful to them,

more so to the following groups of people:

People- Since this study is focusing on human lice, this will be extremely beneficial to the

people as it will give them a sigh of relief from the infestation of lice and a possible solution to

kill their lice and so, they will be safe from having any infection.

Future researchers- This study will be a big help to the future researchers as it will give them

information and techniques that they can use to terminate a human louse if they will tackle the

same topic as ours. In addition, it would be easy for them to conduct a study like this because

they can use it as their future reference, and it will definitely aid them in becoming better

researchers.

Definitions of Terms

Lice - are tiny, wingless insects that feed on human blood. Lice spread from person to person

through close contact and by sharing belongings.

Madre de cacao - is nitrogen-fixing tree that can grow from 10 to 12 meters high. The tree is

referred by many people as a quick-stick due to the characteristic of growing almost right away

just by cutting it and directly planting it in the ground.

Nematocidal- a substance or preparation used to destroy nematodes.

Review of Related Literature

According to LM Torres et. Al 2018, the Madre de cacao (Gliricidia sepium) and sinta

(Andrographis paniculata) leaves extract as botanical animal lice and tick’s remover. The use of

chemical to control lice and ticks of animals especially on dogs is a usual thing to individuals as

this abound in many agrivet store in the market. The unusual way is that many don’t know that

this chemically compounded formula is harmful to animal, environment and to human beings as

well.The body louse as a vector of reemerging human diseases( Didier Raoult, Véronique Roux

Clinical infectious diseases 29 (4), 888-911, 1999)

The body louse, Pediculuss Humanus, is a strict human parasite, living and multiplying in

clothing. Louse infestation is associated with cold weather and a lack of hygiene. Three

pathogenic bacteria are transmitted by the body louse. Borrelia recurrentis is a spirochete, the

agent of relapsing fever, recently cultured on axenic medium. Historically, massive outbreaks

have occurred in Eurasia and Africa, but currently the disease is found only in Ethiopia and

neighboring countries. Bartonella quintana is now recognized as an agent of bacillary

angiomatosis bacteremia, trench fever, endocarditis, and chronic lymphadenopathy among the

homeless. Rickettsia prowazekii is the agent of epidemic typhus. The most recent outbreak (and

the largest since World War II) was observed in Burundi. A small outbreak was also reported in

Russia in 1997. Louse infestation appears to become more prevalent worldwide, associated with

a decline in social and hygienic conditions provoked by civil unrest and economic instability.

Control human lice by using the natural resources in the locality of the city of Tabuk in the form

of plant extracts (Gliricidia sepium and Helianthus annuus extracts). In addition, the researchers

wanted to prove the effectiveness of using the Madre de cacao and sunflower extracts. The
ultimate aim of this research is the introduction of reliable lice eradicators to the locals of Tabuk

City boundaries who can’t afford to buy commercialized lice eradicators. Methods: In every

treatment, it has a constant level of recommended volume of mixed extract of Madre de cacao

and sunflower. The purpose was to observe the effectiveness of the mixture and to determine the

most effective ratio of mixed extract volume. Each trial possesses a time interval of five days.

The total number of lice was determined by adding the number of lice died and the number of

alive after the application. Findings: Madre de cacao and sunflower are effective in eradicating

human lice. The appropriate ratio of combined Madre de cacao and sunflower extract in

eradicating human lice. Madre de Cacao extract: Madre de Cacao, also known as Gliricidia

Sepium, is a plant that is native to Central and South America. A study by Syamsudin et al.

(2017) investigated the effectiveness of Madre de Cacao extract in treating head lice infestations.

The study found that Madre de Cacao extract was effective in killing head lice, with a mortality

rate of up to 90%. Guava leaves extract:Guava leaves, also known as Psidium guajava, have

been found to have insecticidal properties that can treat head lice infestations. A study by

Kamarudin et al. (2017) investigated the effectiveness of Guava leaves extract in treating head

lice infestations. The study found that Guava leaves extract was effective in killing head lice,

with a mortality rate of up to 98%. Aloe vera extract:Aloe vera is a succulent plant that is known

for its medicinal properties. A study by Subramaniam et al. (2014) investigated the effectiveness

of Aloe vera extract in treating head lice infestations. The study found that Aloe vera extract was

effective in killing head lice, with a mortality rate of up to 97%. Combined Madre de Cacao,

Guava leaves, and Aloe vera extracts:Lice Terminator Madricacao is a product that combines

Madre de Cacao, Guava leaves, and Aloe vera extracts. A study by Bawazir et al. (2020)

investigated the effectiveness of Lice Terminator Madricacao in treating head lice infestations.

The study found that Lice Terminator Madre de cacao was effective in killing head lice, with a

mortality rate of up to 95%.

 METHODOLOGY

Research design

This study was designed for experimentation,thus attaining the said objective. To be specific,

this study aims to create a substance that could terminate humanus pediculus (human lice) using

the plant extracts of Gliciridia Sepium (madre de cacao), Psidii Guajavae Folium, and Aloe

Barbadensis Miller (aloe vera). The design in this study was Posttest control group design. It was

an experimental design; its purpose was usually to test whether a treatment was effective. It was

used to compare two or more groups that were formed using random assignment. The

researchers measured the characteristics that the treatment was expected to change. Groups differ

solely on the varrying measurements.

 Treatments Dilutions

Positive
Commercial products
Control

34% Gliciridia Sepium extract, 33% Psidii Guavajae Folium extract, Aloe Barbadensis Miller

Treatment A extract

50% Gliciridia Sepium extract, 25% Psidii Guavajae Folium extract, 25% Aloe Barbadensis

Miller extract
Treatment B

75% Gliciridia Sepium extract, 15% Psidii Guavajae Folium extract, 10% Aloe Barbadensis

Treatment C Miller extract

Subjects of the study

The subject of this study is to create a product that can kill the Pediculus Humanus Capitis

(human lice) using the extract of plants that can be found in nature. This product was produced

through the following plants: Madre de Cacao (Glicidia Sepium), Guava leaves (Psidii Guajavae

Folium) and Aloe vera (Aloe Barbadensis Miller).

Data Gathering Procedure

Treatments
Treatment involved Treatment A (34 % of Gliricidia Sepium extract ,33% Psidii Guajavae

Folium extract and 33 % of Aloe Barbadensis Miller extract); Treatment B (50% of Gliricidia

Sepium extract ,25% of Psidii Guajavae Folium extract and 25% of Aloe Barbadensis Miller

extract); Treatment C (75% of Gliricidia Sepium extract ,15% of Psidii Guajavae Folium extract

and 10% of Aloe Barbadensis Miller extract). The Treatment composed of the sample plants

Madre de Cacao (Gliricidia Sepium), Guava Leaves (Psidii Guajavae Folium) and Aloe vera

(Aloe Barbadensis Miller), these plants was chosen due to its availability. The Madre de Cacao is

washed, smashed, and squeezed to get the extract, while the guava leaves are boiled in the pot

until they are concentrated, and the Aloe Vera is extracted using a spoon.

Control

The positive control was a known commercial product was used because it was proven to

terminate Pediculus Humanus Capitis (human lice). Dissolve the substance with water. The

controlling product were initially dissolved in beakers, tap water, then was introduced into the

three replications of pediculus humanus capitis. Then, it was observed and investigated by the

researchers for a certain time interval and consumed additional time to ensure the efficacy of the

commercial product.

Statistical Analysis
The experimental data were analyzed statistically using one-way Analysis of Variance (ANOVA)

to differentiate the result of the Gliciridia Sepium, Psidii Guavajae Folium, and Aloe Barbadensis

Miller hair solution between the positive control: Commercial Products.

RESULTS AND DISCUSSION

CONCLUSION
RECOMMENDATIONS
 REFERENCES

 Prevention, CDC-Centers for Disease Control and. “CDC - Lice.” Www.cdc.gov, 11 Jan.

2021,www.cdc.gov/parasites/lice/index.html?

fbclid=IwAR3uqoaXuvMDJtLeFLxz9Rc62HvcqNr60l8XTCfYGTP878_vlGpK08OWZj

Y. Accessed 13 June 2023.

 Raoult, Didier, and Véronique Roux. "The body louse as a vector of reemerging human

diseases." Clinical infectious diseases 29, no. 4 (1999): 888-911.

 Balocnit, Divina Alunday, and Joy Grace P. Doctor. “Madre de Cacao and Sunflower as

Human Lice Eradicator.” Indian Journal of Science and Technology, vol. 15, no. 36, 28
Sept. 2022, pp. 1823–1826, https://doi.org/10.17485/ijst/v15i36.290. Accessed 1 Mar.

2023.
APPENDICES