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pK1
pK2
Their names
Their structure
Their three letter code
Their one letter code
O
H2N CH C OH
CH 2
OH
Cystine consists of two disulfide-linked
cysteine residues
Acid - Base properties of amino acids
[A - ]
pH pK log
[HA]
Isoelectric point: the pH where
a protein carries no net
electrical charge
pI pK i pK j
1
2
For a mono amino-mono carboxylic
residue pKi = pK1 and pKj = pK2 ; for
D and E, pKi = pK1; For R, H and K,
pKi = KR and pKj = pK2
Acid-Base Behavior of Amino Acids.
Amino acids exist as a zwitterion: a dipolar ion having both a formal
positive and formal negative charge (overall charge neutral).
pKa ~ 5 pKa ~ 9
10
pKax + pKay
pI =
2
pKa1 + pKa2
pI =
2
pI = 6.0
pKa1 + pKa3
pI =
2
pI = 2.7
pKa2 + pKa3
pI =
2
pI = 9.7
11
The tetra peptide Ala-Tyr-Asp-Gly or AYDG
related to glyceraldehyde
(+) = D-Glyceraldehyde
(-) = L-Glyceraldehyde
All naturally occurring amino acids that make up
proteins are in the L conformation
Priority
SH>OH>NH2>COOH>CHO>CH2OH>C6H5>CH3>H
The major advantage of the CIP or RS system is
that the chiralities of compounds with multiple
asymmetric centers can be unambiguously
described
3-Ultraviolet Absorption Spectrum of Aromatic amino acids;
The aromatic amino acids
tryptophan , tyrosine ,
histidine and
phenyl alanine absorb
ultraviolet light at
280nm ,which explains
the absorption of
proteins at 280nm.