4. Henbcou M. Koxc OCHOBbI BUOXUMUN JIEHVHDDKEPA B Tpex Tomax 1 OCHOBbI BUOXMMUN CTPOEHME VU KATANIN3 Nepesog c axrnuiickoro kata. xm. Hayk T. I. Moconosoi, kala. xm. Hayk E.M. Monoukunoi, kann, 6uon. Hayk B.B, Benosa nog pefakunei akanemuxa PAH A.A. BoriaHoea, w4nex-kopp. PAH C.H. KoveTkoBa a Mocksa BUHOM. Ma6opatopma sHaHuii 2011YAK 578.1 BBK 28.072873 Ha9. Heawcon JL. 149 Ocuons Guoxnnun Jlennmupkepa : 8 31. T.1 / Jl Henscon, M. Kore ; nep. ¢ aiiu.—M. : BUHOM. JlaGoparopux suanuii, 2011. 694. : wm. ISBN 978-5-94774-365-4 (T. 1) ISBN 978-5-94774-364-7 Batre, ManncaMHol @MepHKAHCKHNH YHENLINOH, MOTOPHIE _tony¥HAN veeoGuiee npitinanne RAK TaNaHTAHALe npenoxasarean ynnweperTeTeKOrO Yypomie, paccMoTpeHti cospentennive KONUEHUN GHORHMUN 8 COOTRCTETAN evaweintaureics wieosorwelt 970i wayKH. B tone I paceMorpents: ocHontl GHOXHINH, CaRrb CTpOCHHR GHoNonein © WX peAkUMONMOfi H KOTATITTHNECKOR aKTHBHOCTHIO, CTpOcKG M yHRINI Gnontchipany, wexamnwst GxocuTHarHaLUN, Za naysiinx paGorinnos, YAK $78.1 BBK 28.072873, “SP Hisnaume ocyuecranesio npn dunanconoit nonepxKe Poceutiicxoro donna dynnanerransnenx wecnenosaNiti no npoexty Ne 06-04-6206 Haywnoe uidanue Heascon Avent Koxe Maiixs OCHOBBI BHOXHMHM AEHHH/DKEPA B pex 1oMax Tow | Beayuunii penaxtop Kawa. xin nayk TH. Mowxaena Penaxrop xatut. Onan. Hayk 7 E. Toxemuxuna Xynoxnne HA. Honus Textmsieekuli peaaxtop E. B. ewoxvea Komnborepnan sepetia: 7.9. Buvkosa Howmcano » newars 22.11.11. Dopwaar 84x 108/16. Yen, nex. 1. 73,08. Twpax 300 9x3. 3axe Visnaresscreo «BHHOM. Jla6oparopus snannii» 125167, Mockea, npoeya Axpouopra, 11.3 ‘Teneon: (499) 157-5272, e-mail: binom@Lbz.ru, http:/www.Lbz.ru First published in the United States by W.H. FREEMAN AND CO., New York and Basingstoke Copyright © 2008 by W. H. Freeman and Co. AAll rights reserved. ISBN 978-5-94774-365-4 (T. 1) © Meperon na pycennii ws ISBN 978-5-94774-364-7 BHHOM. Jlabopzropux wit, 2011Nese le)eToag SON ALE CLS Nepeg Bamu, surrareny, nacronpiaa Kina peex GuoxtniKon it MosieKy.Asipnntx GuoAOFOR. Tlowrn 40 ner nasart 6111 onyGanconant Gaects- nusit yueGuurk AaGepra Jlemmyexepa (raps: 24-28) nioceamen npiemuimam nepenasi reneruueckoii wicpopmaui H pery.siuin aKempec- cuit retiog, B Konic TpCTECTO Toma taxoAsTEs TIOC- capi, coabayemnte coxpamicis 1 oGoanancuns, orperi icsanawan, mpeameriotlt ykasaTet Ko BCEMY Kommcxty 13 Tpex Toon. Tpi vaysemm Gwoxnsiint MTatenb eMOxKeT aerko yGeauTucs B JocToNNETHAX aTOrO yueGHIKA, KoTopHtit noaysna ocoGoe upuatanue y enema Crow 3a CHCTeMaTHUHOCTS HSa0xKeHIIL MaTeputaTa. 3aech mprnestens onpererentis ncex ocHovnEx TCPNILHOB 1. KpaTKoe OTMIcaHHe HaNGoace ParKuIDIX SKCHEpUMCHTAAbALBIX MeTOOB. OcoGo cacityeT OT- METITD BeNHKONIETIMBIC TOCTpanIMN, BITHMATEN Hoe HayICHHIe KOTOPHIX slaeT BOSMORKHOCTH HHO.NHICC HOHSTh CYTb OUMCHIBAEMBIX LIPOLCCCOB.* He comnicnaenes, «ro ara kitira Gyaer Rechsta voctpeGopantioli. Hopoe wsmanite ta PyCCKOM s3Bs- Ke aTOTO BCeMDHO HaBECTHOTO yUCHHNKa OKDKCT HeouenuMylo HoMOMLb cTyZeHTaM Macruparran, Haysialonuist OHOXHMINO, a TaKOKe MperionanarensiM. Bee naysinse corpyaunkst cMoryT ¢ Tomball Mt ys BOALCTBHEM YsITATH M LepeusITHRATD wirTepectissii MaTepiiat, Kaki pas OTKPHIBaA HTO-TO OROE 1 =nOGUMoll Haye, Tepewox Kuru smomien T. T1. Mocosoaoii (npeancaopne, rap 1-7, 14-16, a raroxe ¢ppar- MeHTH TeKCra, MosmuRUMHees! B S-M_ tant), HH. Barponoii (raps 8, 18, 20, 22), B. B. Berounin (canis 9, 13, 17), E.M. Monouximoii (ratanut 10-12, 21), HI.JI. Aprorkwmoil (r1awa 19), O. M. Azexceenoit (capa 23), O. B. Echpemenkonoli (razanit 24-28). uit Ypanie- A.A, Bordauoo, axademux PAH C.H. Kouemxog, xten-xopp. PAH * Btexere anti cenakn Ha anekrponune pecypeet @. Cu. caity http://bes.whfreeman.com/jehningerse/OREO ORC eal tel ear aurun coe 12 13 14 15 16 TOM 1 Octosbi 6HoxHMnn Yact I CTPOEHME Ml KATAIM3 Boga AmmHoKHcnoTsI, nentHapbI M GenkH Tpexmepxaa crpyktypa 6enkos: Oynkynn Genkos ®epmenret Yrnesoei w ranko6uonorua HykneotHabl K HYKMeHHOBbIE KMCAOTbI Texwonormn Ha ocHoBe wncbopmaynn 43 HK Junuapt Buonormueckwe Mem6paxbi m TpaHcnopT Brochruanwzaqna TOM 2 Yactb II BMO3HEPTETHKA M METABONU3M Tpwnywnst 6nosHepretuKn Tankonna, rnioxoHeorene3 W NeHTO30CpoctpaTHBIN Nyt NpwHynne! perynaynu MeTaGonn3ma Ha NpuMepe MeTabonn3Ma rnlOKO3bI wrnwKorena Unka nMMoHHO KuCHOTEI 17 Kara6onw3m KMpHbIX KHCAOT 18 — OkWCneHMe KHpHEIX KHCROT v o6pazoBanve Mo¥esHHbI 19 — OkucawtenbHoe doctbopunuposanne ¥ horotboctpopunuposanne 20 — Buocunres yrnesonos y pacrennit v Gaktepnit 21 ~~ Buocunte3 nunujos 22 — BwocunTe3 aMMHOKMCAOT, HyKNeOTHAOB W CBARAHHBIX C Mx MeTABONN3MOM MONEKYA 23 Mvrerpauwa # ropMonanbHan perynayna MeTa6onu3ma y MneKONUTaWOUyMx TOM 3 Yacte Il NYTH NEPEQAYH MHOOPMALMK 24 ‘Tevet w xpomocomet 25 Mera6onwam HK 26 = Mera6onw3m PHK 27 Mera6onv3m Genka 28 ~~ Perynayna rexHod aKcnpeccun Tipnnomenne 1. Coxpaujenun v o603Ha4eHHA, wcronbayembie B 6HOXHMKH Mpnnomenne 2. Orsersi x saqavam Tnoccapnit HicrounMin mnmocrpaynit Npeamernpiit ykasatenbpeng J. Henbcox poanaca » Dspmoure, urrat Munuccora, CLA. Hoaysii crenenb Gakananpa no XMM H GHOsIOTHH B KoAIEAKe CR. Onadha 1964 r. Banos aneceprannonnyio paGoty 10 GuoxuN w MeaUNICKO! mKONe Crandbopaa nog pykowoactsom Aptypa KopuGepra. Toaysitn cmimenauio Tapapackoil meyuniuuieKoli uiKom1, paoraa umecte ¢ 1Onanuuos II. Kenncsu, Ko- Topi Gur OAM HS HepEBIX aciMpanTon Jle- nunwxepa. C 1971 r, Heascon paGoraer B yuit- nepcntete Buckoucun-Maauicon, ¢ 1982 1, — 8 AurxHOCTH Mpodbeccopa 6HoxmMutH; B HaAcToRMee apea — anpextop Uenrpa Guostoruyeckoro 06- pasonans. Hayaunie untepecis Henucona cussannt ¢ IByYeHHES MepenasM cHrHAAOB, peryzmpyioutx ABIOKLII PECHINCK H gIIOUNTOS Y upocTeiimix pora Paramecium (B ocKontiom ero paGoTit nocas- eH onpertesteHHHO post (bepMeNTOR, fH YaCTHOCTH Mporemnimas, B nepenave curiasa). B nay4noii pynne Heascona Hcnosbayior CoupeMcniBte Me- TOW HCCHEAOBAKHS: PASLMINVINBIE MCTOsLBL OMCTKA Gera, HNMYHOOFHYECKHE MeTOMbI AMALIA, 91CK- TpoHaA MKPocKONHs, a TaKXKe TeHCTHYECKHIC, NoaekyaapHo-Giroaornueckne m aneKrpodistto0- iweckite MeTOABI. Heabcon Hapecren Kak BeIzaroutniies J1eK- top upenoaavareab, Bonee 40 ner on Kypupy- eT ACKUMONNEE KYpCH HO GHOXMMHT aA CTY AeHTOR, CHEILAAAMpYIOUXes! B pasaNannx oGiacrax GuoaornuccKoit Haykn. On unraer Take seKUMH HO Kypcy GHOXHMEM cTyAeHTaM, Hay YANOULHM MeRCECTPUNCKOC AEAO, HEKIWH AK acnupanton uo crpykrype H (pyuKisin Mem 6pan 1 no MoacKyaspuoit Heiipoduanoaormm. Viumuarop opranmsannit siorux crumensutii © YeabW NOAEPKKU AKTHBHO paGoTarOMLNX cTy- ACHTOB 1 ACTIMpALTOB. Ba CBOIO BHAOUYWCR ipenonapatensckyw jestTenbtioctb Heascon 10- ayaa Harpas, B TOM sHKczTe Npemino Jpeiieyca Jug negarorou-ysennix. Umeer spanne mover nor uporpeccopa Vauwepewtera Buckoncna. B 1991-1992 rr. Heabcon 6111 npuraauenin npopeccopom # KoaesKe Cueamana. Bropas crpacth yucuoro — MeTopust HayKH, BOT HOWeMY: ov unTaeT cTyseHTaM Kype HeTopHEE GuOxHMEE 1 cobupaet coGcTBcHyIo KOAMeKKMKO CTapin- MIX HaySIIbix HIICTpyMenTOB. Apsna JI. Henecon v Maiixn M. Koxe Matikn M. KoKkC poanscs » Ysmuurrone, urrar Jleaasap, CLA. C caworo navaza nayse ist Guoxnsant xtmra JlenmuvKepa oKasana 1a M, Koxca cross cittptioe Bansinne, ¥T0 nepeopit- cirrmponasia ero naysitute mrrepecst oF 610.10- rik Gnoxusitt, To oKOHMaIMH yHMRepertTera ur, Jlenawap B 1974 r. Koxe siinoaina amccepra- \utomtyio paGoty B yunBepeuTere Bpantciica nox pykonoxcruom Yunusima TL. Jbkenkea; ¢ 1979 1. paGoraa # Crabopackom yHunepcutere Hoa py- kononctuom PoGepra Jlemana. C 1983 r. M. Koxe paGoraer B yunnepenrere Buckorrcnn-Maancon, a ¢ 1992 r. — 8 xowKUOCTH mpompeccopa GuOXHMUMH. Auccepranua Koxca nocesiucta npimentcritio Mo- TEAM OGUCTO KHCAOTHO-OCHOBHOTO Katanga JU waysenns epsenratuaniix peaxusii, B Crou- chopackom ymuncpcsrrere on santimasien Hsyyeit- ex qbepMettron, ywacTnyionux B peKomGuHartsin FenoB, yaeaHB OcHOBHOe BUAMaHHe HayucHi Geaka RecA ycranownemmo smexannsma nepe- Hoca nit JJHK; paspaGorannie Koxcom meroztet soienenna 1 anaansa Geaka RecA ucnosnsywor- ca 20 cux nop. Fnapnoii Temoii B Hayunoit pabore Koxea ncerga Gusto nayyenne cbepscHtos, y4a- CrbyloMHX B reHeTHYECKOi pexoMGmHaNUIN. B ynunepeutere Buckonenna Koxe pyxo- noant Gombutoii 1 akruBHolt Hay4Hoi rpynnoit,[8] 06 aeropax xnurn paGoratomieli p oGactH attatnozori, Tonosorut H aneprerHkH pexomGunauin renos. OcHonnne Tent Hay'MHBIX paGoT cro COTPYAHIKOB ocBaIe- nt Mexarianass o6Mexia nitreit HK ups yuacram Geaka RecA, ponn ATP p cucteme RecA m pery- asin pexomOnmausionnolt penapannn. Yacrna- Ho rpynna aausita nayuene KaeToK Deinococcus radiodurans, o6nanaiounx yanpuresbio MoULNOli cucremoii penapauun, 1 npHmeHeHnen stoi cH- crest penapauit 8 GuorexHonorun. Ha nporskenmn mocnemel Gonee 4etrep- TH pexa Koke copmectio ¢ J[. Heascoriom mpeno- aaeT Kype GuOXHMM CTymerTaM Mt sHTTacT Tex no ctpyktype u tononorin JJHK, JHK-Geaxonsis soaumouciicrausm 1 6uoxmMnt peKombutaus acnupaitam. Acnuparti nepporo roxa cayuraiot ero Kype © npodeccuoriatbriolt orsercraemioctH Gyayuux yuerax. 3a cHOM HayHHBIe HCCHIeAORANHS: H mpentozaparenncxy reserbitocrs KoKe Gen narpaxaent npemueli JIpelidbyca sat nenaroron- yuertorx nt npesmeli xosmaitun Eli Lilli wo 6noaoru- ‘ecKoii xiinitn n 1989 r. Xo66n Koka — caxononcrB0, KOJLACKLUHONNPORATTHC BUI Ht YHACTHE R TLTAKUpORA- HHH 37lauiMit [UIA HayunpEx HCCICAOBANHT.sa OB O HayKe nactynputes XX1 p, np noayennn Kaaccn- Neckoro ecTecTneHtio-Hlaysnioro o6pasopamis Ne yaeasieres: AOMmkHOTO. BHUMAKHS dbiuTO- corbin naykus t cpriocorcKst OGoctoBannsis. 11a yuntix Teopni. Mockoasky mpeanonaracrea, 170 B Gyaymes wo: Gyaere sanmnterncs Haysnoli Aestrenb- HOCTbIO, asec YMeCTHO ellle pad OCTAHOBHTECH Ha cyTH TaKUX nonsTHii, Kak MayKa, yHeHDIH 1 Hayy vi meron. Hayka nlonpaaymenaet oanonpemenitio ct10co6 aGmiogerins 3a OKpY>KANOMUU MIIPOM, a-TAK>KE Cy My naHihix 1 Teoputt, BbETeKaIOMNUNX 13 Toro Ha6.1I0- aetia, Mott nayk 1 ec ycriex Hanpsimyto caezyior 3 Tex ce HloxOxeHti, KOTOPEIe MOXkHO MIponeptT, Hecaeays IPHONE ABCHILA, KOTOPKIE MOxtO vaGsnonatb, HaMepHTTb 1 BOCTPOWEARECTH, HAN pest Taras TeOput, UMCIONUIE MpestcKadsTENDITYIO CHLLY. Tiporpecc naykn 6asupyercs na dpynmanterrratHioMt pitutuime, 0 KOTOPOM PenKO roROpsT, HO KOTOpIii upespusiaiino Barker 9TOT NpIHIUHN sakaOvaeTCA 8 MOCTOsHCTHC saKOHIOR, YuIpABAIsHOUNX CHUAN H swacuitani po vceli Bcenenmoii. Jlaypeat Ho6e- sescsoli pent Kak Movio taanaut ator upsmiu suioctyatom oGnexrimHOcTH upHposbt>. 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Haywax meopus — 210 yxxe wexro Gonvuuiee; 970 zea, KoTOpas B AOCTaTOMNO;l CTeneHH MOATBEpAK- aeTCA OKCEPHMEHTAIDIKM nyTeM 1 OGpsICHICT octonityio sacrp Ha6.nonenit onpenescHHoro poa. Haysnras Teopis nceraa octowatia a dpakrax 1 MO- 2keT GHITb MpORCPCtia, MOBTOMY TeopItio MOXkHO ME NCHIITh pH AATBHeTMNX HCCaeOBAKHAX. Ecan Ha- yurias. reopiist MuroroKpariio mponeperta tra dhaxrax s HOATBEpKACHA MO MHIOTHM TlapaMeTpaM, e€ MORKIIO Apnnsith KaK naysnbiii part. ‘Moxkno ckasare, To Haysnible Hytest COCraB:LuOT conepxanne naysnnix crareli, KoTopuie nyGamKyiorest B Hay4uBIX akYpHANAX HO PeNeNaHNL jPYTUX yuerEAX. Exeroaio 6 muipe 8 16000 naysiirx akypHatos ny- Gannyerea oxono 14 muuuiona craveli, 1 ool a copmanutelt moxer BocHaEsoHATbCs OGOI YesIOBEK. Yuewsie — vto moan, KoTOpIe MpHNCHsHOT Ha- yan MeTon. K rlostiantino oKpy>ka1outero Mupa. Yue Mim te CranowsTes: MpocTo mpHt MonysensM Haysnoti CTOHCHH KLIN SHAT, a OTCYTCHHE TAKOBLIX HE MC liaeT NeOBeKY wHecTH BecoMBit BIAIAA B pasBITTHe vaykut. Hactosmemy yuenomy cBojicrserio merrarucs cchopmyanpouarb Kakyto-1160 rhuioteay B OTRET Ha HosWIEHHE HoBBIX jaHHDx. FIpHuHMaeMe Hay4ETe THMIOTESb! AOADKHBL OCHOBHIBATKCA Ha SMEpHeMBIX 1 BOCHPOHSBOAIMX aGmMONeHHIAX, UpHes HITaraTD Tit HaGmozienia yuentait nompKert aGcAMOTHO HeCTHO. Haysnpiii mero sxmouaer neabtit HaGop uyreit hiccaenonatnsi, npitiem 10G0i nyt MoxeT MpHne- CTH K HaysNomy orKpurtio. [yt om zunomeses do akcnepumenma — yuersiii cpopmyaupyer runoresy it lipoBepsier ce akcHepuMeHTabHBIM MeTonOM. Miio- THe GHOXUMEYECKHe HponeccEs, C KOTOPHIMH GHOXE- MHKH CTAIKUBAIOTeA B Choc exerteBHO!i paGoTe, Gouin OTKpEIT UMeTHO Taki Opasom. /LKeliMc Yorcox 1 @pericne Kpux yeranosn crpyxtypy ALK; Gaaroxaps atomy orkperrino Guia chopmy- JIMoRatta FHMOTC3a 0 TOM, HTO CllapHBaHte OCHORA- unit JHK sexi 8 ocione nepenaynt reneritueckoit sibopMaliii npH cHHTese HOsMyKseOTHLELAX 110 crenonaterbuocteii, Ita cnnoTesa NoMOrAa B OT- «psitint PHK- JH K-nonumepas. Yorcon u Kpuk orKpsian ctpyxtypy HK, uc- hoaEsya MeTOR Modequposanua u pacuemoe. Oun HE UIPOBOAWAM HUKAKHX CaMOCTOSTeABHEIX AKCIIC- PHMeHTOB, a HeroAbIOBAAN OKCepHMeITaNHIe JAHHMe, MOAYYeHIBIE pallee ApyruMM yyenbine. Tlekoropute cmenuie yucnitie » KavecTBe Hay4Horo MeTona us6pann Memod na6aodenuii. Orxpurrs, CACHAN MaBeCTHBIMH NYTeMECTHEHINKAMH, B To siicae Yapibsom Jlapputiom npu nyreuecTBust na «Buraiee #1831 r., nossonnan costars Kapry Ha- neit nanerss, onnicarb ce oGrTaTescit w HaMeHTAN[10] Heckonsxo cnos o Hayke aim npencrasiiens 06 oxpyakatotest muipe. Co- BpeMeHHBIe yueHIe, HecmeAyIOUIMe MOpCKHe rity- Gun H.0m Hoctimaioute pakeTo K ApyrHM niraKe- TaM, HAYT TEM 2ke nyTeM nlognanust. Ene ou nyTb, hanommiaiommit nyTh rHuoTesEI—oKcnepHMertTa, ociiowan na Merone 2unomeaa—dedyxyus. Kpux curaa, “To jOsKHa cytIeCTRORATS MOJeKY.ra- ocpeniink, oTRCHaioMlas 3 nepeHioc HncbopsaLH satpiinoli PHK p Genox. Dra renuanesas riino- Teaa ripiipesta k oTKpBITiiIO Matoniom Xorsation Tostow 3amesiukom Tpancuopritix PHK. He uce naysiibte OTKpLITHs ConepmiatoTes: er10 Tuanly; "acto BarKIIyIO porb urpaer wamyuygus (nposopaueocmy) yaeuoro. OrKpertus memmuit- ama Anexcanjpos Daesuuirom w 1928 r. 1 KaTa- mmmineckoii akrupiocr. PHK Tostacom Yexom b Hayate 1980-x rr. 20 HeKoTOpoii cTenenH GLI caacTanBuinn caysaiinocran, omako 3TH yueHLIC 6bLnHt FOTORKI K BOCUPHSITIO 1 CTIOMBaORAHI coonx onkppiTiii, Hematonaxnyio ponb Moxker cbirpart 11 adoxnosenue. Tak, MeTon nosuMepasiioli nermoit peaxuuit (TILLP), Koropstit reneps sanit- MaeT eHITpalbHOe Meco B GHOTeXHOsOrHCCKHX liccaenoBannax, Oui costan Kop Mionaucom & HOPBINC BAOXHOBEILIA BO PEMA HyTCIICCTHUS! NO Cesepnoii Kanmopruint 8 1983 r. Hepesiiesrcnute asin yr k naysuiomy orkpLt- HO MOr'yT HOKagaTHCS HIKAK HC CRSAaNIIbIME MEKILY coboii, oniaKo y Hix CCTh RaxkrIBe OGuNe CHOICE: Bee on HanpasneH Ha HsyseHue upmpoast. Bee omit oupaiorest ta eocnpouseodunoe nabnodenue 11 (um) skcnepumenm. Bee wan 4 akcrepuMentath- Tine nlonTBepAKACHIA, CHANNDIE C HayNHBIM 1O- HCKOM, Moryt 60tTb MIpoRepenttt H BocrIpourancncHt ysienbimit B suoGoii TouKe crera. Bees Haysnibisin TIMOTeSaNI It OTKPHITHAMM MoryT BocHOABIORaTD- oa apyrne yuctie 21a CoananiNs HoBBIX rHnorea conepmienus: Honitx orkpsrruii, Bee on mputtocstr snchopsauunio, KoTopat CooTACTCTAYIOMTUIM O6pas0M Honomtwict HHtpopsanHourioe GorareTHO HayHoro anarnst, Mayenne yerpoiicrea satpa — TsaKesrast pa- 6ora. Ho net Gonee saxpareiBalomero 1 AOCTOIHOTO. 3aNlsITHsL, NeM MBITATKES XOTH Go HACTHIHO OHSIT yerpoiicrpo Mupa.eS Tel fe) eT epnoe ananne Kunrn «Principles of Bio- chemistry», wauucannoit Anp6eptom Jle- numurxepom 25 ner nasaa, nocaysKHINO OT- upaBuoii TOUKOHL H MomesbIO AIA MOCHERYIOULNX Neruppex gnanuii, 3a ueTRepTh Beka GuoxiMTte- ckie 3HaHHA HeHMoRepHo pacuupuaen. nas Marb usTh eT Haga Me Gen pacuippowan wt aii reo, He 6111 mpopeaen pemtrenocrpyK- ypu aniaana HM oAHOrO MemGpainoro GeaKa I He cyIecrRoRasO 1 OHO‘ HoKayTHO‘ MEM, Toasxo-Tonpko Goin OTKpHITH PHGOSHIMD Ht BHC apena texnomorus' TIILP, a apxent Gut npuseane OTACABIBIM HapcTLOM opratinaMon, Cerogits 110 ave rexler4eckie NocactoRATesIbHOCTH NyGaMKY- JoTeH ekeHteneMbHO, a HORBIe GenKOBMe CrpyKTYpHt Tospasiotca it Toro waule; yueHBIC Cosa THICANIE adHBX HOKAYTHBIX akIROTHEIX, NTO cnlocoGcTByCT yerteuniomy pasprrrino Guoxmati, ¢buraitomorni a Neanumnbt, B 9ToM sto ManaKtiit KEITH mpen- crannents (bororpadpun 31 Hobesenckoro naypea- 7a, KoTOpwe TozysHLIH CHOH MpeMmt B OGacTIE NINN, (PHSHONOLMN HI Mes yKe Mocse auxoga B CBeT HepBOrO H3sAHIA Onuoit ita satay Karkaoro MocaeaytoMlero 13- anita 68470 B KaKoli-To CTemteH orpasTs NoTOK noctynaouteli Honoit wHcpopMaut, Ho npH aTOM He meperpyaitrb naanne, Beats 10 atoll Kure CTy- AetTbt HacTo BHepBBIe stiaKoMaTcs ¢ 6HOxUMHE;t. ‘Twareasnsiit oTGop Hicopmanin rospomnt sas HAMOXUTb OCHOBUBE NHIIUITI GHOXHNUEL H TIpTt STOM TORG/LaTb 0 3aXBATHIRAIONIUX HayHIBIX 10- crinkenusx nocneaunx eT, O6 HX s1aMeNAtH ZU 6yaymero senoncuectna. 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(Take HOMCHEHIA BBISBIBaIOT y TAC xKes1aMIe HOMOAOCTE tt RepHYTECA K HavaTy Hamiell HaysHoii 1 Mexarornse- ckoii kapsepes, Boab Hauta kniira — He enmHicrBcHta Belltb, KOTOPas MocTaperta 3a BCC 9TH TOWtbL.) B kaxk0M NOBOM H3/aHHM MbI CTapatich Coxpa- tint» Te 2OcTONHCTHa Kir JlemELDKepa, KoTOpHIe CENA CC KNACCHYECKHM YYeGNMKOM, 4 HMCHHO, MCTKOE H3/OAKCHHE, TIMATEABHOE PasbACHCHHE TPysA- HUIX BOUPOCOR H OMuCaNe TeX MeTOLOB, KOTOPLIMI nombaytotca coupemeriite 6ioxmMuikn. Kak apropit Mut paGoraem BMecte yake Goce 25 ner W Kax tIpe- nosaparean — yxe NOWTH CTosbKO 2Ke. 3a BCC ATH Tomb THCAH HAUINX cTyAeHTOB B yHHBepeHTeTe Buickoncuti-Manticon Gxt HenceaKaeMBIN HICTON- HiKoM Hell O TOM, KaK CulenaTe Kype CoBpeNtent- Hoi Guoxnan Goace nousrTHun. Hau cryjenrat oGysat 1 BAOXHORAIAM Hac. Mul oveHtb HazeeMcs, ‘To ava Kura, NocpAMeHHad ABAALATHUATHACTHEH ToROBIUME HepROrO Manali, HOMOXkCT OGyaUTh 1 BAOXHOBHTS HORKIX CTYAeNTOR, Hay'aioNUNX 64OxH- Mito, H, BOSMO2KHO, SaCTABIIT HCKOTOPBIX M13 IHX 110 mo61Tb GHOXUMIO TAK, KaK MOGHM eC ME‘Ta KITA — MIO KOLIEKTHBLOTO Tpysta, H ce BHIXO B CBCT Obi GbI HeBOSMOXKeH Ges TO”- AepRKH COTPYANHKOL HaaTeNDCTBA Freeman and Company, Kotopue noanepxupami Hac Wa ween nytH cosnanust KinrH. Penn Poccumon (rannutit penaxtop) 1 Kettr Ap (orwetcruenttstti penaxrop) opranusosinan penensiin, BHecat MHOXCCTRO LICHIBIX upeTOXeHNit, oAGALpHBAAL Hac, BeAM K Kes H FepouNecKH (XOTH H IIe Bera Yerieumo) sactawamn wac yRAaMBATHCA B OT- berenHie Bpesennnie pawn. Han woyiaomuriics Penaxtop JIns Fenep KakniM-T0 o6pasom yMyAps- ach NPOABHTAT BEIMYCK KIMTH, HECMOTPH ta BCE uponymtemmte cpokH H BHesanmue HsMeneHua, priest sex1421a 97O Cee BeerAAMHIM OGasHHEN Macteperuom. Mut Gaarojapitbt Bukit Tosacesuin 3a suisaii Kivi t Mapme Koon 3a samevatean- noi maxer. Ham BHOBS HOBeaIO BCTPeTUTHCS A pa6ore ¢ BbIRAOULNMCH TexHHYECKHM pesakTOPOM Jlunazoit Crpewx, Koropan nogrorosiuia BbinycK. Beex natn wsnannit Principles of Biochemistry (a TaKxe Ba paHunx Wanannsa Biochemistry camo- ro Jlenmyoxepa). Ee nomoutp 6ecucuma, 1 060¢ €@ pukocHopensie K TeKCTY Beera Cro yayumta- er. Ham Taioxe tions nouesto paGorarb BMecTe ¢ Mopranom Paiiaom, Kotoprtit nomoran aM mpHt bbinyeke AByx npemuayux Hanan Kuen. Mot Gaaronapum Jleny Jurimbo Beru 3a ee nomout 8 noaGope 1 pasmemennn otorpaduii n Haka Tu MouKo # Yarn Kaeny sa Kooprtmaniuo paGorst BCeX YHACTHNIKOK MpockTa. Met TaKKe BEIpaKaeM GnarogapHoctb aupextopy no mapKeruury JIc6- 6u Kaep 3a opranisaiuno mposarki m Mapkeruura kHurH 132 ee npexpacuutii Mop. B Manncone namust reppin peaakropom 1 KPUTHKOM UIpHt TlogroToBKe weex Hszanuii KHI- rit Gouna octaetca Bpyk Coxrseat. Ona neppoit upocmarpiisasa raph pyKONICH, HoMorata B non ToTOBKe TeKCTa 1 PHICyIIKOB, CaemUIA sa Coraco~ Baritem OTHeADUMX parMeHToB, 3a enIICTBOM HOMCHENATYpB H1acKOBO, HO HACTOINHNO Hac MON rowta. Mut 6zraronapun lean Jlyserm, Tenepb paGoraiomtyW 8 ynnpepertrere Ho1o-Mexiiko, Koto pas, KaK 1 Ips NoAFOTOBKe MpenLAyUTerO HanAMEE KHMTH, MPOwta KaxKMIOe COG KOPpeXTypEI, Hama MHOTO OMINGOK 1 BHIeCTA HEMULIE UPeIOKCHN, KoTophle yaysuIHAM KUNTY, Hopoe ocpopauicnie KUEN, BkMOUas H306pa 2KCHTIC HOBLIX MOeKYAADHIX CTPYKTY, BEITOMHICHO sitect, 8 Manucone, Anastom CrefinGeprom, koroppsit BHC MHTOTO TlOmIesHEIX MpejIOKeMHi, MOSBOANBLX caezare ns06paxcnits Gone Harnanntann. Btacro- SITIES HAAN TAIOKE CCT HIOGPAReHNA MOMIEKYA, bbINOMEHMNe jL1A TpeTEerO H HeTBEpTOTO Hanan 2Kan-Hsom Crpo — nauiun Koaneroii 13 Maaucona. Ham oven noueao, 70 € Hast B paGore nprmusa- aM yyactie Takue TanantmBue mom, Kak Bpyx, lenan, Awast 1 Kan-Ho. Mor Tawxe ovens mpranarensin Bpaiiany Yaiiry ws Maccayycerexoro ynumepcntera 8 Bo- cToue, KOTOPHt cocTapHA HoBHe aaqayit HO ara AIMBY AKCHEPHMEHTANbUDIX AAMIDIX [LIA KarKOM raaBbt KHHTH. Muorne nan xoaners: nposwasan Gosbmoii MHTepec K XOAY BHINOANEUMA UpoeKTa 1 OKA Ham GombIyIO MOMOMIb, BHIOCA NoNeSHBIe KOM- Meirapum. Oco6yi0 Guaronapnocrh Mb xorHM suipasims Jlopency Anaepcony W13 yHnwepeuTe- ta Buckoncun-Maancon, Axedeppit J. Seko ns Kaanopuniickoro yunsepcurera 8 Can-JInero, Tbxexy Kupuy 1 ero ctynenran us Kasnopuntii ckoro ynmepertera 8 Bepxan, a rawxe jlane Sui oa, Ilay-Yyan (Llepiin) Teaii, Maiixay T. Kymexn Wx Koseram (nepeucnentbim unDKe) 43. Kas opntiickoro yunuepentcra 8 Hpraitne. Muorne HOMOFAM HaM MOALOTOBHTS H BEINYCTHTh 3TO TR TOE HA/IAHHE KHIH, BHOCH OALESHBIC COBETHI, KOM- MenTapiti H aanewanis. Mia mpisnaresbie Been, KOTO Mbt HKJHONHUIN B ITOT CHHCOK: Pusiapa M. Amaanno, Yiuaepcumem Buckoncun- ‘Maducon Jlynaa E. 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Marrsoc, Dapaaxaxoewnecruti ipaxynomem Ysusepcumema Muccucuru Bewxawun Jbx, Max@apaana, Ti ypuusepeumem Cusmaa Braronapwocm [13] Anant Menon, Meduyunceuii xoxted xe Beiaa Kopueasa CaGexa Mepwianr, Kanupopnuticxutt ynusepcumem, Tloc-Andmcenec Corr C. Mop, Yiusepcumem Bocmona KunGepan Moypu, Yiawepcumem Bpoyna Jleiia Myaamme, Texaccxuti AM ynucepcumem Cenaitr Heram, Kanupopnuiucxute ynuaepcumen, Tone-Bun Aaaen B. Huxoxcon, Yuucepcumem Tewns Xupomn Huxaiino, Kasupopmuitexuis yuusepcumem, Beprau Tbxelime HramGu, Yuuoepcumem Bucxoucun-Msducon Tumorn @. OcGopn, Kazupopuuicxuis yrausepcumem, Hpaain 2Koae P. Hepee-Kacrneiipa, Yinwepcumem Ceousu, Henanus: ‘Teppu Maarr, Yinwwepcumem Powecmepa Benn Moroaenscxn, Yiusepcumem ITv0-Hopxa, Teneseo Zpwowaran Honnep, Yiuvepcumem Bucxoncun-Msducon ‘Tomac Hayzoe, Yiiuwepcumem Bucroucua-Maducon Tpxex Hpeiice, Yiusepcumem Muaueana Anna Ponammuexa-Tanans, Yuusepcumem Apkansaca Pou Paiinec, Yiueepcumem Buckoucun-Msducon To A. 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OcHOBOM AIA NOCTPOEHHR ITOTO RDE- Ba ABIAETCA CKORCTBO KYKNeOTHANBIX NOCRenOBATEMAHOCTeH PAGOCOMKOK PHK BKYTPK KAKAOA "pyri; ve Gonkitle cxoncrao nocnenoBarenbiOcre#, Tem OnuMe paCTlONaraNOTCR BETBM, Tak TO PaCcTORHMe MeMRY NEMA BETBRMK OTPAMAeT CTeNeHS pACKONEHMA NBYX NOCREROBaTENEHOC- ‘Tel, Oynoreneneveckie RepeBbs TaWNe MOYT GurTe OCHOBAKEA Ma CXORCTBE MochenowaTENdHOC- ‘Tu aMiMoKucnoT 8 oTmensioM Genke. HanpyMep, Ha OcHOBe CpaBHeHWR ocnenoBaTensHOcTeh Genka GroEL (aktepvannuii Genox, Koropsth ywacreyer ® cGopxe Genkos) mocrpoexo ppeBo, ovasaitoe Ha pic. 3-32. Ha puc. 3-33 MpencTasnevo exoNceHicycoe> RpeBO, MpH TlocrpoeKHM Koroporo ja nocrumernns Gone TOMNBiX pesynbTaTOB SBOMOYMOHNOTO CpoRcrea rpyrin opra~ .¥3MOB WcrionbsOBabbx NECKONEKO MapaMeTpOB CpaBHeHUR, TaKWe KaX BbIMIEMTePESMCHERHAE,[20] 1. Ochosts 6uoxwmnn Bee acunnte oprannisarst a overpoder Coneprasicnera) Herowank oMepran ig EE] — Amvorpodes ; BE] (yracpocies Coy — ener 5 LvanoGaxrepi Tlypnypise Gaxrepiit z Pacrema Bencnnie GaxrepHt i & = Puc. 1 Boceranommennme cocammemiis Oxaenenmese Coneprn xnauiecnis cocaumen sit) eer Opranorpodat Gnepen ses opranieexiny cocammeniit) Cepnuie 6axrepun Boz bituicrs0 ipoxapior Bonopontite Gaxrepun Bee nexbororpodatse aykapnoris shoe Are ONS 3. Opraxusmat MOMHO KNACCH>MIIMPOBATS B COOTBETCTENM C TEMK KCTOMHMKAMK 3Hep- wm (convieunisi CBeT NMG OKMCREKME XMMMMECKHX COeAANENMiT) K YInepORa, KOTOPHE OHM VCHOnkayioT ANA CHHTe3a KeTOYRNX BelleCTB.. oreytersyer, moaysaior auepriio nytes eperioca nek TpoHOB Ha Hrrparr (c o6pasouaunsem Ny), cyabepar (€ o6pasouaunes HS) nit CO; (¢ o6pasowarniem CH,). Muorne oprarvant, anouononmporannine 1B ataspo6uBIx ycaoRHAx, smuoTeH oBnicamneLs (crpornia) anaapo6asiz B npiicyreTenN KntcROpona init orator. LIpyriie— paxyaremamueneie astaapo- 64s, ot MOryT IIT Ht Gee KHEIOpost, HB ero NpHeyT- cron Opratirasts MoxHO Kracci(pinupoRaTs B coor BeTCTENH ¢-TeM cHIOCOGOM, ¢ LOMOMSIO KOTOPOTO OF onywaior aHepriio 1 yralepor, NeoBxonNMDe Lit cutresa Kcrosnoro semtectra (pute. 1-5). B sapiicu- MocTH OT HCTOUIIITKa SHeprUH BULLET je GonBIITIC rpyuinst oprattitamon: botorpodpet (ot rpex. trophe TTanuc) HenouBaylor comeUHbIit CRET, a XEMOTPO- chet no6sinawor aHeprino nyrest oKHe-teHHA TorLTN- Hix Moaeky.1, Ipyniia xemotpodHiix oprartaMon, NagbinaeMbix sATOTpOdhaMa, criocobtia OKNICAST He- opramuveckne nemiectra: HS 0 auemerttaptoii cepst S,S10 SO? ,NOz 10NOg ,a Fe® 20 Fe. Oprano- TpOdet oKnCAsIOT pantie oprammeckie Beule- Tma, Haxomsnumecs B oKpyxarouicl nx cpente. Doto- Tpogbo i XeMOTPOcbDL TaKKE MOAHO PasmemtTe Ha TPYNNU R cooTHCTCTRNH ¢ TeM, MoryT J OFT HenOmb- aoparb m KaNeCTHe HCTOUIKA yrnepos CO, (aBTo- Tpodpis) sun komen NonyaT® yrsIepoa 1a opraHite- CKUX TurraTetbHbIx ReMMeCTE (reTepoTpodbbl). Baxrepma Escherichia coli — HawGonee m3y4eHHan GakTepun Crpoenne kierkn Gakrepuii user oGuume crpyktyp- bie NepTbi, a TakxKe HeKOTOPLIe rpyniionDIc cob oct (pic. 1-6). B Hopsanintx yeaonustx. E.coli abAserca Geso1acHLiM oGurarevem KHIUeUHIKA yeronera, Jumma ce KICTKH OkONO 2 MxM, JHAMCTP yrs weniee 1 wks. Kaerka satintena BHemIHeit MeN Gpanoii, a BuyTpeHHAA IWiaawarnuecKaR MeMGpaHa OrpantnyiipacT pocrparicTBo, B KOTOPOM sakmONe- Net UurrontaaMa 1 HyKeoNt, [pocrpaicrno MeKity buiewneli 1s syrperneli sicmGpartastt satis70 ToH- KIM, HO MPOYHBIM CloeM TOMUMEpoR, HasbIBAeMIX TenTHAOrAINKaHAMH, KOTOPHe LIPHAAIOT KZIeTKE 2KCCT- Kocts Hu onpeneenuyio chopwy. Thiaasaraeckas MenGpania UMecTe C BHEIIHKMH TO OTHOUICHINO K Heli caosmu oGpanyer KreTouHyto O6oT0UKy. [pos HocTb KulcTKAM apxcii UpIMtaIoT HoMMEpEL JApyroro Tuna — nicesnonentimormxart. [rasmarivecKas MenGpana Gakrepitit CocToHT 113 TOHKOrO swHOIiTOrO cao aMmm108 ¢ GeaKOBEIMH BKTONeHHIAMIT. Me Gpannt apxeii Hmexor anratornantoe CTpocHte, ontaKO x MIU HopasttrenbibIM O6paaom oTattwalOTeA OF uumit08 Garcteputi (em. prc. 10-12). Bigtronnasne E.coli conepaxutes oxono 15.000 PuGocom, OF AecsTH 10 THICANH KONI KaDK-AOFO Ha upuepno 1000 pasmsuetx bepmenron, a TaKoKePuGocomni. axtepnamunie pu6ocoms Meme SYKapMOTUMECKHX, HO HIpAIOT TY 2Ke Poss — yNACcrAyIOT ncHnTeae Geaika Ha ocHope Matputs! PHK, Krerowias o60x04Ka. Y pasmax siszon Gaxtepmii nwcer Paanyio etpyxrypy. Tiveumnso: wenGpaica Cait nenuauerninata Bayrpennast Buytpennsys Ipaorpmuaremume Ij Tee Gaxrepi. Burennivit mem6pana, cxofi nerrijormxatia Her neuer wenGpanu, Goaee toxcrutt exoit newrunorsmKania Reche Upanto6axrepun. Apxen. Tanorpiaremime Ter anenmeli menGpann Gaxcrepir, nao cxolt caoii HemtHaormHKana nenritorsnkana; pacntonoxen capyxH PaswNTas DHyTpeHIAR — OT TIMaaMATHNCCKOF jpaiva, conepxaiuias = MeMGpatibt dhotocumreraveckae urs [[Cacitnenmaoramcana | 1.1 Mpmnynnes oprannsanwn kneran [24] Hyraeoua. Conepxur eamncTRenttyio suEHyto Kosibuenyn) MoaeKyaty JUHK. pic on / Kryrun. HeoGxomueatt {a9 nepeawioKcnHet CTH. Puc. 1-6. O6uyne crpyxrypnixe snementes Gaxtepnanbibix KneTOK. B cBnsM c pasnnNtsM CrpO- exueM wneToxHOH OGONONKK HeKoTOpK sy6aKTepun (rpaxnonomMrembee GaKTepHH) YAep- xusaior Kpacutenb mpy oxpauiusanMn no Tpamy, a Kexoropue — Her (rpamorpuyarensibie 6axrepun). E. coli ormocurca x rpaMorpuuarensitsm Gakrepuam. [vaxo6axtepn mpencrapna- vor co6oii sy6aKrepnK, Ho oTnKYAIOTCA OCOOH CTpyKTYpoH BHYTPEHHeM MeMGpaHst, B KOTOPO! noxanwzosanet borocuHTeTHyeckwe MurMenTL. KneTouNbie OGonoYKM apxei W rpaMmonomH- ‘rembiwx ay6akTepHit OR 3nekTpOHHBM MUXPOCKOTOM BMITMAAAT CXOAHNM O6pa20M, OBKAKO expoerue wx MemOpannieix numegos x rlonktcaxapyoe 2aMeTHO pasnuvaeTea (CM. pC. 10-12). ‘oxo20 1000 opranmectnx BemtecTs ¢ MomeKyatapHoit swaccoit sienec 1000 (meraGoarns 11 koxaktope!). B YKIeOMLAC pachosIOXeHa EMKHICTHEMTIAA KONBLICHAS wosexy.ia JK, a B uHTOrUIasMe, Kak Hy MHOrHX /)py- rx Gaxrepiii, Berpevactest oguta YUH HecKOMbKO Go- ace MenkuIx KobUcRHIX MoneKyat JUHK, nasninaeMnx naaamiurann, B ipuponubix yenouusax weKoToppie iiaaMMab oGecreMuBaloT ycToiiuBocTh GakTepitit K pagmNMnIN HaxORANUINCA B OKpYoKaHOMeit Cpete ToKcHHaM u anTHGuOTHKaM. B aGopaTopHiix yeno- buax ott oackyaii HK neobsriaiino yno6tet a1 ocymiecraicniia akchtepuMenTatbstbrx MansTy-tst0K[22] 1. Ochoets 6noxummnn unomuan wnetaa PuiGocoms ~ anapar cmrexa 6enka Tepoxcucos pasnaraior uepoKenss _» Uprrocxener tloatepkunact erpyktypy ~~ eneniar, criocoGcrayer nepensioxenito opraniesit Jiusocoms yunsToxaior -— BHYTpHKAcTOUBIe OCTATKH ss “Tpantcnopriise vactanies (wesiy.tb1) ‘obectiesnpaior TpartcloprupoBky Gemtkos Be WamiutO8 Mexaly OP, amapatom Tomssokn LS i nawwarnseceoH meGpanof Anmapar Fonysoxit ocyitectasiaeT mpoueccHnr, ynaKouKy w focTasky Genkos x apyriM opranenaam ¥.1K BRIBOANT ioc Kateri SO. Brameuii stvtoraamariseckuti peruxy.rym C > (FOP) cayrxtir Mectom cvrresa sMni1708 \_ wera6oamana nexapers repias coro \ \ ‘Aapouuxn — Mecto cimrresa ccmmersponariat, \ (Konmmene JIHK ¢ \ pH6ocomnoii PHK: Genxoss) oF wu:T0- \\ Mlepoxonarii \ FInpo conepxart ronan \\\. suabnaasmariectati perniyaye \ ret (xpowarin) P) crore \ Tinaswaririecxas wen6panta \ \ GLIDP) © ywacror citresa \ ornenaer kaetky OT oKpyxkaiomell Slnepitas ccpeatbi, peryampyer noctynenne oGoroNKa / PyGocose — Usrockener WobutereniMe BeuecTs \. Mirroxoupmit — mecto oxnenenis \ TOTANBHEEX MOMICKY1.€ ‘oGpasonattnens ATP ‘S. Xaoponaacrs eobupaior connewmntli ‘caer, oGpasyior ATP. yracworis Annapar Tomax hanya kpaxmata enyscarr _ MecToM xparieniia yrneDono8, ‘oGpasonapurixcs & peaynsrare gorocuttesa ‘TwsaKowun — yuacren ‘cpeTosapiicHmoro cuttresa ATP Katetoxnast crema OGectiewsBaer KECTKOCTE hopmy irerkut, samuutaer ee OF OcMoTHMecKOrO 1HOKA Baxyoan ~ mecro paatoxennst ~ Kucrosnas crema coceawelt kierkH i nepepa6oren wakpoMoneKy, Tanokentcosta — meeto xpanieniia a Takoxe MecTo xpanientist chepMentros raHoKcinaTHoro Wika era6om#Ton TLaassoncenta o6ecnesimaer Kouraxt Mexkiy ABYMA PACTHTeMbHBINH KTIETKANH en Puc. 1-7. Crpykrypa aykapnormveckoit Knevkn. Cxenarneckn wso6paMenst KIETKH AByX OCKOB- ADK THTIOB: aaepxY — KMBOTHAR KNeTKa, BHUSy — pacTwTenbian KneTWa. KneTKm pacTerMit O66 Ho WMeIoT 2MaMETp OT 10 NO 100 MRM, a MHBOTHNE KneTKK — O75 NO 30 MXN. CFPYKTYDH, BRINENCH- le MPACHLIM UBETON, MPHCYTCTEYOT TORSO B MHBOTHEE WK TONBKO B PacTHrensitax xneTWaK. B wnervax syrapnorintecknec mnpooprantnamos (Mamprmep, MPOTHcTOS 1 TpH6OB) HMeIOTCR TaKME me CTPYKYpEL KaX B KNeTKaX PaCTeHHI: W MBOTHIX, XPOME TOTO, BO MMOTHX 3 MUX TAKME ECTS ‘rreymanwsnposaae opranenny, KOTOpHe 3pech Ke uSOGPAMeNLI.1.1 Mprnysnes opraknsauynn xnernn [23] Tbe itemprebyrnponanie Toworessanyn ca Hutsxas exopocrs weverpudyruposamn 1000 ¢, 10 ma) a Hisonuxamyeckoe wentprepyrnposanne >> Cynepraranr uenxpubyrupyor ¢ (@rpaamesre eaxapoasi) Gated autconodl exoportbin (20.000 gg, 20 aavttt) . ~ | Henrpnpyruponanne (Cynepnarant | jerrpmiyrupyior ¢ eute nee BRICOROH cKOpOCT (000g 14) Cynepuaranr uentpnepyrupyior c> oa conepaatT uene KAETRM, S012, urrockenerid, v savaerusieere OGpaxcu ‘NeMbpait f Ocaaox . conepyrr Tpaanetrr surroxonapion, Cymep-—caxaposes “INSOCOME!, arate nepoKcHcoNnt } Corepmur Menee | Gane pacrnopn. lori conepaur arc Geman ReMMONEE arene sakpocomt Po (@parvenrrst DP), 7 Menke Ty KONIONeHT weauiytot Ocaaok conepaxstr puGocosses, a pyrite makpomovnexy nut B76 5 4 8 201 Puc. 1-8. Pasnenenne cy6xnerounbix crpykryp TRaHM. [Ina nonyvexuA BHyTpAKneTOMEAX opra- Henn Twakty HaMpuNep Never, cHavana MlORBepraioT EXAKWYeCKOH TOMOTEKMSANM AAR Paspy- lWeeHR KneTOK u RHCITEprMpOBAKMs x ConepAnMOrO B Gyibepe. PacrBop caxaposst OGecrIeNMBaeT "MpaKrnveckH Tanoe Xe OCMOTHNECKDE MABNEHWE, K2KOE CYECTBYET BHYTPM KNETONHAKX OPrakeMN, ‘10 Mpemcercreyer NpoxuxnoBeHo B opranennst Bon, ux pan6yxanwoo x paspyuienvo. a) Kpyn- de Memkwe wacriIas B CYCTIeHSHM MONO PasneNkee YEHTPAGYTUpOBAHMEM c pase CKOPO- cramn. 6) Yacruusi pasnMsHOi THLOTHOCTH MOKHO PasjeNWT WOMMKHHYECKMM YEHTPKGYTHPOBA- ven. B naxion meTone ueHTpMDpyMaRe MPOOMPKK sArToNKROT PACTBOPOK, IMGTHOCTS KOTOPOTO pospactaer ceepxy BHU3. [na TlonyyeHwA rpagueHra IMOTHOCTH rOTOBAT, HaMpuMep, PACTBOps C pastioi normeHTpaywei caxaposu. 3aTeM Cmecb opraHten NOMEMaNOT 8 BePXHOW YaCTS MPOGMPKH XK neNTpHdyrupyier ¢ BEICOKOH cxopocrH. IIpx stom OpraNennex OCAMAAIOTCR B FpaARMeHTE NO ‘Toro yPOBHA, MOTHOCTS KcTOporo TOUHO cooTBeTCTEYer Wx COOcTseHHOR. Mocne UexTpAdyrHpo- BAHU KAMABH CHO MOKHO OTOOPAaTd 13 TPOGUPKH OTAENBHO.[24] 1. Octobe 6noxwmn OCH LUPoKO HenoMBayIoTeR MosTeKYAspITAMH re- HoruKast. Bombumicrso Gaxtepuit (pkauovas E.coli) CytllecTayIOT B BILE HHZMBILAYATBHBIX KIeTOK, HO nexoropsie nivtet Gakrepiii (1anpitmep, Mirkco6aK- Tepu) AemMonctpupytor mpocreiimee , O6pasya MHOroKuIeTOUNBE arperatés BykapnoTmeckne KneTKM ConepyaT pasHoo6pa3sHbie Mem6paHOceAzaHHble opraHennel, KOTOpbIe MOKHO BLIAeNMTb H HCCHeOBaTS Tunmunue aykapvoruueckie Kerk (pic. 1-7) Bo oro pa3 Mpewbmiaior no pasMepy Gakrepiampubic it Burnio uMeior sutanterp o7 5 10 100 sek, a 1x OBEN BTHCSUH ILM MELLIHOMN pas Goxrsme ove Gaxre- piatbubex KtetoK. Otarirenbioii ocobemocrsio SYKApHOT AILARETCH HALITUNC apa H MHLOTONHCHEHIEBIX chasanHbIx ¢ MeMGpanoii opranea co cneundpie- cxuivn ynKIULANAE: surTOXOUApHi, a#LIONAasMarHT¥e- ckoro peTuky.tysa, Kosmutexca Touibsbkit, 1epoKCHCOM 11 amgocom. B paCTHTeabIIbIX KUIETKAX, KPOMe TOrO, ConepxKates BakyosH Ht XTopotactel (pite. 1-7). B WWETORIASMe MHOTHX KIETOK MpHCyTCTBYIOT CpaAHy.THt TUT KaNeADEH, CONEPIKAIINE ZaTACHBIe MHTTATETUBE beMeCTRA, TAKHe KaK KpaXxnaut s1 KHL, Suawirrenbubi RKTaL B paspirTHe GHoxuAMT hecain paGoru AnbGepa Koa, Kpnicruana ne Zhiova 1 Zokopixa Tlazaae, nocpamentibe MeTonaM paate- JICHISL KETONHEIX OpTaHeNUI — BaLKHOFO ITaNta B BBs TCICHHH HW HByYEUHH byHKtuTit 6HoMaeKy.1 1 Gostce KpyuHEX KeTOUHLIX CrpyKtyp. Ipu oGuIuHoii npo- neaype ¢ppakunonnporaniia (pyc. 1-8) KnerkH 11H ‘TANI, HaXOMITIECH B pacTBope, ToMoreHsHpyior Bb Markux Yenosiax. 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Pas- Gopxa, c6opxa 1 oKariigaiuist cex Turon ¢bsvtawestron peryaupyiores apyruni Genkanss, KoTopHe HcoGNOnH!- Mbt JL18 CBSSbIBaHNLA ¢bILTAMCHTOB HIKE tiepeMeMcHnist oa HIEK MUTTOILWaAMATINNECKTIX Oprane-Lt. Onneannias nme crpyktypa coorpercraycr ctt- Tyan, Ha6monaouelica B ayKapHOTHNECKOI KIeTKe € ceuaroii crpyktypolt rreii u cackHoii opramtan