The Bacteria Around You and the 01

Effects of Antiseptics

The Bacteria Around you and the Effects of Antiseptics

James Pinard

SBI3U

Ms. Devlin

Oct 26th, 2022

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Effects of Antiseptics

Introduction:

This Experiment Was conducted to test the effects of bacterial spread against the use of antiseptics.
Similar to all other living things, bacteria need food to survive and develop. By spreading/producing
spores, binary fission, and conjugation, bacteria may multiply. When a cell divides to become two in
binary fission, it replicates its DNA, then puts a barrier between the two sets of DNA, and splits into two.
A plasmid copy is transferred from one cell to another during conjugation. The last stage of reproduction
is known as spore generation, which takes place when bacteria release a copy of their DNA through
spores. (Personal Interaction, Ms. Devlin, 2022) This lab's objective was to observe how bacteria develop
and respond to antiseptics. According to the project’s theory, germs would proliferate until there was no
more food available, and the bacteria that included antiseptics would find it difficult to multiply and
grow as quickly.

Methods:

Kindly see the lab experiment description for the supplies and background needed, "The Bacteria
Around You and the Effects of Antiseptics." Nothing was altered. Similarly, for the procedure please See
the Lab experiment description and guide, as little to no changes were made.

Although Please consider the following alterations: For portion B, four different regions were swabbed
rather than just one. The end result being eight total different subjects/areas swabbed instead of the
four same areas.

The Results:

Table 1 (no antiseptics used):

Source of Bacteria Day 1 Day 2 Day 3

Max Bolden little growth was irregular shapes of the The irregular shape
found. The bacteria bacteria grew rather grew rapidly as orange
developed a significant fast as the rounder bacteria formed.
smooth and irregular formations slowed.
shape in addition to
some spherical and
punctiform forms.
Leg Lift The bacteria grew into The irregular shapes of There were hardly any
clusters of smaller the bacteria grew spherical bacteria
smooth shapes and larger, as the smooth remaining as the
then more varied ones still grew but at a irregular form
shapes. slower rate increased. Black mold
began to form
Hayden Fitzgerald. The bacteria Grew into The rounded shapes The bacteria grew
many round, smooth began to grow larger slightly bigger,
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Effects of Antiseptics

and punctiform and spread slightly. maintaining it’s general

growths. size and shape.
Wood Shop Button, Similar to most The round shapes of Round shapes of the
bacteria in this study the Bacteria grow bacteria grew and
the Bacteria devolped larger. Orange spots became prominent. As
into smooth, rounded, and growths started to primarily punctiform
and punctiform shapes. appear. shapes grew on the
orange spots

Table 2 Antiseptics:

Source of bacteria Antiseptic Day 1 Day 2 Day 3

Fogel’s Chair. Alcohol The bacterial With certain forms Bacteria became
shape changed to having rounded red and black. The
one that was edges, the shapes majority of the
compact, grew more erratic. corner had been
spherical, Alcohol was taken over, and
rounded, delaying the rate there were many
irregular, and at which the more erratic
punctiform. There germs seized forms, giving the
was now a Small control, and the edges an
zone of inhibition. surface was appearance of
coated with being lobate.
smooth features. Nearly all of the
inhabitable zone
has vanished.
Ms. Graveline. Listerine The bacteria The bacteria The bacteria grew.
formed into curled continued to
irregular and grow, and more
punctiform shapes smooth round
as well as a small shapes appeared.
smooth irregular
shape and round
smooth shape.
There was no
visible zone of
inhibition.

Blake/Blake’s Hand sanitizer The bacteria took Compared to the The zone of
Mentum. the appearance of other samples, the inhibition
spherical, smooth bacteria grew decreased
forms. A sizable more slowly, and considerably, the
region of the zone of bacteria increased
inhibition was inhibition barely significantly, and
seen. changed. some yellow
bacteria surfaced.
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Effects of Antiseptics

Water fountain. Water The bacteria The bacteria grew The bacteria
formed into a continuously, the continued to
irregular shape originally rougher grow. Showing
became wrinkled irregular shape little change from
similar to became a before but sharing
furrowed Land. smoother growth. an overall change
With little to no from the first day
inhibition Zone with the second.

It was discovered throughout the experiment that the bacterium in the first petri dish was spreading
throughout the dish at a steady pace. It was observed that the bacteria couldn't grow close to the
antiseptic in the second petri dish. The finest examples of this were in sections A, when the germs were
taken from a chair belonging to Mr. Fogel, and section D, where the bacteria were taken from Blake, and
hand sanitizer was used as an antiseptic.

Discussion:

This lab's goal is to observe how bacteria multiply and how antiseptics impact this process. There is no
way to test that portion of the idea because the experiment was stopped before the bacteria ran out of
food, but the other half of the hypothesis is supported by the fact that the germs grew when given
antiseptics. The bacteria it interacted with may develop more slowly due to the antiseptics, while
bacteria that weren't exposed to them could multiply rapidly and freely. This itself implies that
antiseptics can be used to stop the spread of germs. Utilizing additional samples as well as other
antiseptic kinds and dosages might enhance this investigation. It would be wise to utilize one variety of
bacterium for all the antiseptics in subsequent studies.

Conclusion:

The fact that the bacteria with antiseptics proliferated less than the germs without antiseptics provided
evidence in favor of the idea. This experiment demonstrated how antiseptics may impede bacterial
growth. Additionally, this study demonstrated how some antiseptics are more potent than others. In
contrast to the bacteria treated with Listerine, the hand sanitizer-treated germs displayed a zone of
suppression for all three days, not surprising as the purpose is to kill harmful bacteria.

Sources:

Personal Interaction, Ms. Devlin. (2022)

Personal interaction, Eric William Gray. (2022)

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Effects of Antiseptics

Personal interaction, Jaden Michael Robert Lloyd. (2022)