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Molecular Identification of Cabbage Whitefly, Aleyrodes proletella L.

(Hemiptera: Aleyrodidae)

Conference Paper · May 2018

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Abdurrahman Sami Koca Mustafa İmren

Bolu Abant Izzet Baysal University University of Bolu Abant İzzet Baysal, Turkey
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Halil Kutuk
Bolu Abant Izzet Baysal University
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 Uluslararası Tarım Kongresi 3-6 Mayıs 2018 Komrat/Gagauzya/Moldova (Sözlü Bildiri)

Molecular Identification of Cabbage Whitefly, Aleyrodes proletella L.

(Hemiptera: Aleyrodidae)
A. Sami KOCA1* Mustafa İMREN1 Halil KÜTÜK1
1
Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Abant Izzet
Baysal University, Bolu, Turkey
*Corresponding author: a.samikoca@yahoo.com.tr

ABSTRACT
The cabbage whitefly Aleyrodes proletella L. (Hemiptera: Aleyrodidae) is recognized
pest of Brassica vegetable species. All of the species found in the Aleyrodidae family are
phytophagous, and many of them cause economically significant damage in cultivated plants.
Whitefly specimens were collected from cabbage fields in Düzce province of Turkey. The
identification of the whiteflies is made from the pupa stages and it is very difficult to determine
the morphological characteristics of the whiteflies and to make their diagnosis. In addition,
identification of white flies using conventional methods isn't rapidly. The aim of this study is
to make the whiteflies identification using molecular methods. The DNA isolation of each
whitefly was performed and then the COI region of mtDNA was amplified with universal
primers LCO1490 and HCO2198 by use the polymerase chain reaction (PCR). Ten sequences
were obtained from the Düzce samples and all tested whiteflies were defined as Aleyrodes sp.
To confirm the identification of Aleyrodes species, the sequences were compared with the
identified COI sequences of mtDNA from GenBank. It has been shown that this DNA locus
can only identify at the genus level of whiteflies. It is necessary to use different gene regions
outside the COI region of the mtDNA in the diagnosis of whiteflies or to develop specific
primers.
Keywords: Cabbage Whitefly, Aleyrodes proletella, Molecular Identification
This study was financially supported by The Scientific and Technological Research Council of
Turkey (TUBITAK Project No: 116O921)

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