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Abstract Seed viability and germination of Eryngium foetidum using soaking duration and chemicals treatments was studied at the
field laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University during July 2010 to June 2011. Seed treatment
with growth regulator (GA3 500 ppm+Kinetin 50 ppm) and soaking of seeds enhanced seed germination in the laboratory. Chemical
treatment (copper oxi-chloride 0.2%+tetracycline 1 000 ppm) had no significant increase of seed germination over control. Untreated
or pesticide treated seeds exhibited lower germination than growth regulator treatment. Increase of soaking duration enhances and
increased seed germination up to 96 hours. Consecutive 96 hours soaking and drying (8 hours soaking and 4 hours drying) of
Eryngium seeds treated with GA3 500 ppm and Kinetin 50 ppm gave the maximum germination percentage 74.7% in laboratory and
enhanced germination by 2~3 days over control.
Keywords Eryngium foetidum; Seed treatment; Soaking; Germination
experiment was conducted to standardize the seed Seed treatment with growth regulator and chemicals
treatment and priming for increasing germination (Tetracycline plus copper oxy-chloride) showed
rate. significant variation in respect to germination
duration and weekly germination as well as total
1 Results and Discussion
germination rate (Table 1; Figure 1).
1.1 Germination performance in the laboratory
Table 3 Combined effect of seed treatment and soaking on weekly germination of Eryngium seeds
Treatments Days to first Weekly germination (%)
germination nd rd
2 week 3 week th
4 week 5th week 6th week 7th week 8th week Total
C1D1 20.7a 0.0c 2.0kl 2.7i 2.7 1.7 1.7 1.3 12.3hi
C1D2 18.7bc 0.0c 5.0ijk 4.3hi 2.7 1.0 0.7 0.3 14.0hi
C1D3 17.7de 0.0c 7.7ghi 6.0ghi 3.0 1.3 0.3 0.0 18.3gh
C1D4 16.7f 0.0c 9.3gh 7.7f-i 4.3 2.0 0.3 0.3 24.0fg
C1D5 15.3hi 0.0c 13.0ef 10.7d-g 6.3 2.7 0.7 0.0 33.3e
C1D6 14.3j 1.0c 16.0e 11.7def 6.0 2.0 0.3 0.0 37.0e
C2D1 18.0cd 0.0c 9.0gh 13.7cde 8.0 4.3 2.0 1.7 38.7e
C2D2 17.0ef 0.0c 16.0e 16.0a-d 7.0 5.0 1.3 0.3 45.7d
C2D3 15.7gh 0.0c 24.7d 19.0abc 8.0 2.7 0.3 0.0 54.7c
C2D4 14.7ij 1.3c 33.7c 19.7a 5.3 2.7 0.3 0.0 63.0b
C2D5 13.0k 4.0b 44.3b 19.3ab 5.7 1.0 0.0 0.0 73.7a
C2D6 12.0l 8.0a 50.3a 14.0b-e 1.7 0.7 0.0 0.0 74.7a
C3D1 20.7a 0.0c 1.7l 2.0i 2.3 1.7 1.0 1.0 9.0i
C3D2 20.0a 0.0c 3.7jkl 3.7hi 2.3 1.3 0.7 0.0 11.3hi
C3D3 19.0b 0.0c 6.7hij 5.3ghi 3.0 1.3 0.7 0.0 17.0gh
C3D4 17.7de 0.0c 7.7ghi 6.7f-i 2.7 0.3 0.3 0.0 18.0gh
C3D5 16.3fg 0.0c 10.3fg 8.7e-h 3.7 0.7 0.3 0.0 23.7fg
C3D6 15.3hi 0.0c 12.7f 9.3e-h 3.3 1.0 0.0 0.0 26.3f
Significance * ns ** ** ** ** ns ** **
CV% 5.15 105.62 10.87 28.08 35.94 47.28 80.90 120 10.89
Note: Means followed by same letter or without letter in a column are not differed significantly at 5% level; *, ** and ns indicates
significant at 5%, 1% level and insignificant, respectively
germination with GA3 treatment and soaking. The July 2010 to June 2011. The experiments comprised
lowest seed germination rate (9%) were obtained with two different factors such as growth regulator
from the pesticide treated seeds with no soaking treatment and soaking duration.
which was wonderfully lower than absolute control.
This might be the cause of an adverse effect of Germination test were conducted in normal room
pesticide in seed germination in Eryngium. Control temperature with eighteen treatment combinations of
(no soaking) with or without pesticide treatment two factors. Factor A comprised of three seed
showed the delayed (20.7 days) germination in treatment viz. growth regulator (GA3 500 ppm+
Petridis. Kinetin 50 ppm), pesticide (copper oxi-chloride
0.2%+tetracycline 1000 ppm) and control (distilled
Seed treatment with growth regulator (GA3 500 ppm
and Kinetin 50 ppm) with 72 to 96 hours soaking (8 water) and Factor B comprised of six soaking levels
hours soaking and 4 hours drying for 6~8 times) are viz. 0, 12 h, 24 h, 48 h, 72 h and 96 h soaking having
found better for enhancement and increasing seed 8 hours consecutive soaking and 4 h drying was used
germination in Eryngium foetidum. in the experiments. All the chemicals according to
the treatment were prepared in the laboratory and
2 Methodology used after application of soaking treatment. The 18
The experiment was conducted at Seed Horticulture treatment combinations consisting Factor A (Seed
Field Laboratory of Bangabandhu Sheikh Mujibur treatment) and B (Soaking) are shown in Table 4.
Rahman Agricultural University, Gazipur during
49
International Journal of Horticulture 2013, Vol.3, No.10, 46-51
http://ijh.sophiapublisher.com
Table 4 The 18 treatment combinations consisting Factor A (Seed treatment) and B (Soaking)
Treatment combinations Factor A: Seed treatment (C) Factor B: Soaking duration (D)
C1D1 : Distilled water Control (No soaking)
C1D2 : Distilled water 12 hours
C1D3 : Distilled water 24 hours
C1D4 : Distilled water 48 hours
C1D5 : Distilled water 72 hours
C1D6 : Distilled water 96 hours
C2D1 : Growth regulator Control
C2D2 : Growth regulator 12 hours
C2D3 : Growth regulator 24 hours
C2D4 : Growth regulator 48 hours
C2D5 : Growth regulator 72 hours
C2D6 : Growth regulator 96 hours
C3D1 : Pesticide Control
C3D2 : Pesticide 12 hours
C3D3 : Pesticide 24 hours
C3D4 : Pesticide 48 hours
C3D5 : Pesticide 72 hours
C3D6 : Pesticide 96 hours
100 seeds were placed onto 3 layers of blotting hybrid seeds of gladiolus, J. Maharastra Agric. Univ. Publ., 25(3):
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International Journal of Horticulture 2013, Vol.3, No.10, 46-51
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(Eryngium foetidum L.) influenced by plant growth regulators and Samaan L.G., E.E.T. El-Baz, M.A. Iraqi, and E.F.A. El-Dengawy, 2000,
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