UNIT OUTCOMES:

At the end of this unit, the student should be able to:

1. Perform the proper handwashing, soiled glove removal and proper

donning and doffing of PPE
I. Hand Hygiene
1. Handwashing and use of Alcohol-Based
Sanitizers

2. Alcohol-based sanitizers are for when

your hands are not visibly contaminated

-Spread sanitizer over cleansing area

-Rub space between fingers
-Continue until alcohol runs dry

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
I. Hand Hygiene
1. Handwashing:

a) Antimicrobial Soap
b) Paper Towels
c) Running Water
d) Waste Container

Note:
Remove any jewelry in the hands, wrists,
and fingers. Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
Handwashing:

1. Stand a few inches in front of the sink

2. Turn on faucet; place hands in running
water
3. Use soap and work up lather
4. Scrub for at least 15 seconds. Make
sure all surfaces are scrubbed, esp. in-
between fingers and knuckles

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
Handwashing:

5. Rinse hands from the wrist downward

to fingertips

6. Dry hands using paper towel

7. Use paper towel to close faucet

Video: Hand hygiene tutorial

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
II. Soiled Glove Removal
Proper Removal of Gloves:
1. The wrist part of one glove is
grasped by the opposite hand

2. The glove is pulled inside-out and

off the hand

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
II. Soiled Glove Removal
Proper Removal of Gloves:
3. The removed glove is placed in the
gloved hand.

The fingers of the non-gloved hand are

slipped under the wrist of the remaining
glove; do not touch the exterior surface

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
II. Soiled Glove Removal
Proper Removal of Gloves:
4. The second glove is pulled out

5. The gloves must be thrown in the

appropriate waste container

Video: Soiled Glove Removal

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
III. Donning and Doffing of PPE
Donning of PPE
1. Gown should be worn first

2. Mask should be worn next.

3. Gloves should be worn last.

Note:
Goggles, Respirators, Face Shields are worn after the mask, but before
the gloves. Gloves are always worn last.

Video: Soiled Glove Removal

Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
III. Donning and Doffing of PPE
Doffing of PPE
1. Gloves should be removed first

2. Gown should be removed next. Gown should be removed

from the shoulders toward the hand.

3. Mask should be removed last. Remove the mask by

holding only the string.
Note:
Goggles, Respirators, Face Shields are removed after the gloves.
Image Source: Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition
Video: Proper Donning and Doffing
Textbooks

1. Ebuen, B., et.al. (2020) Learning Guide for Principles of Medical

Laboratory Science 2
2. Strasinger, Susan King (2019), The Phlebotomy Textbook, 4th Edition