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Parts of a microscope with Categories
functions and labeled diagram Agricultural

November 5, 2023 by Prashant Dahal Microbiology (12)

Edited By: Sagar Aryal Bacteriology (124)

Basic Microbiology
Having been constructed in the 16th Century, (60)
microscopes have revolutionized science with Biochemical Test
their ability to magnify small objects such as (114)
microbial cells, producing images with definitive
Biochemistry (155)
structures that are identifiable and
Bioinformatics (21)
characterizable.
Biology (184)
Derived from Greek words “mikrós” meaning
Biotechnology (28)
“small” and “skópéō” meaning “look at”.
Book Reviews (1)

Table of Contents Cell Biology (99)

What are Microscopes? Culture Media (67)


Structural parts of a microscope and their Difference Between
functions
(89)
Optical parts of a microscope and their
functions Diseases (29)
:
Microscope Parts Worksheet Environmental
Compound Microscope Parts Worksheet Hints Microbiology (7)
Compound Microscope Parts Worksheet Answer
Key Epidemiology (23)
Light Microscope Worksheet Food Microbiology
Inverted Microscope Worksheet
(49)
Dissecting microscope (Stereo microscope)
Worksheet Genetics (79)
How Does Microscope Work? Human Anatomy and
Parts of a Microscope Revision Questions
Physiology (70)
(FAQs)
Q. Define a Microscope. Immunology (115)
Q. State functions of a microscope. Instrumentation (63)
Q. Diagrammatically, identify the various parts of
Journals (1)
a microscope.
Q. Describe the functions of each part of the Microscopy (28)
microscope you have drawn above.
Molecular Biology
Q. Differentiate between a condenser and an
Abbe condenser. (66)
Q. What is the magnification power of the Mycology (33)
objective lenses?
Q. How does the eyepiece compare to the Parasitology (25)
objective lens? Pharmacology (14)
Q. Why is the rack stop included in the
Phycology (2)
microscope from the factory, and can it be
replaced? Protocols (9)
Q. What is a magnification power?
Research
Q. Differentiate between the fine and the coarse
adjustment knobs. Methodology (19)
Q. List down the 18 parts of a microscope. Staining (29)
Q. List down the three structural parts of a
microscope. Syllabus (18)
Q. List down the 17 parts of a microscope. Universities (4)
References
Virology (50)
:
What are Microscopes?
Microscopes are instruments that are used in
science laboratories to visualize very minute
objects, such as cells and microorganisms,
giving a contrasting image that is magnified.

Microscopes are made up of lenses for


LEAM Solution
magnification, each with its own magnification
Fluorescence
powers. Depending on the type of lens, it will
Microscope
magnify the specimen according to its focal
strength.

Their ability to function is because they have


been constructed with special components that
enable them to achieve high magnification levels.
They can view very small specimens and
distinguish their structural differences, for
example, the view of animal and plant cells
viewing microscopic bacterial cells.

There are different types of microscopes like light


microscope, dark-field microscope, phase
contrast microscope, electron microscope,
fluorescent microscope, etc.

Microscopes are generally made up of structural


parts for holding and supporting the microscope
and its components and the optical parts that are
:
used for magnification and viewing of the
specimen images. Modern microscopes have
additional electronics and display devices. This
description defines the parts of a microscope
and the functions they perform to enable the
visualization of specimens.

Structural parts of a microscope


and their functions

Fat Cells: Definition,


Types, Structure,
Functions, Diseases

Solanaceae Family
(Night Shade or
:
Potato Family)

Water Purification
Methods and Steps:
A Complete Guide

Endothelial Cells:
Definition, Types,
Structure, Functions

VRBL Agar (Violet


Red Bile Lactose
Agar)

Figure: Diagram of parts of a microscope

There are three structural parts of the


microscope i.e. head, arm, and base.

1. Head – The head is a cylindrical metallic tube


that holds the eyepiece lens at one end and
connects to the nose piece at other end. It is
also called a body tube or eyepiece tube. It
connects the eyepiece lens to the objective
lens. The light coming from objectives will
bend inside this tube. In binocular
microscopes, they are adjustable so that the
viewer can adjust the eyepiece for maximum
visualization.
2. Arm – This is the part connecting the base to
:
the head and the eyepiece tube to the base of
the microscope. It supports the head of the
microscope and is also used when carrying the
microscope. Some high-quality microscopes
have an articulated arm with more than one
joint, allowing more movement of the
microscopic head for better viewing.
3. Base – The base is the lowermost part of the
microscope that supports the entire
microscope structure. It provides stability for
the microscope. Illuminators, light switches,
and electrical wiring systems are fitted in the
base.

Optical parts of a microscope


and their functions
:
The optical parts of the microscope are used to
view, magnify, and produce an image from a
specimen placed on a slide. These parts include:

1. Eyepiece – The eyepiece (ocular Lens) is


closest to the viewer’s eye. They are located at
the top of the microscope. This part is used to
look at the specimen. These lenses come in
different magnification powers from 5X to 30X,
but the most common ocular lenses are of 10X
or 15X magnification. They magnify the image
for the second time.
2. Eyepiece tube – It’s the eyepiece holder. It
carries the eyepiece just above the objective
lens. In some microscopes, such as the
binoculars, the eyepiece tube is flexible and
can be rotated for maximum visualization for
variance in distance. For monocular
microscopes, they are none flexible.
3. Diopter Adjustment – Diopter Adjustment is a
control knob present only in the binocular
microscope that is used to change focus on
one eyepiece. It is used to correct any
difference in vision and compensate for the
differences in vision between the viewer’s two
eyes.
4. Nose piece – A nose piece is a movable
:
circular structure that houses all the objective
lenses. It is also called the revolving turret. It is
connected to the body tube and lies just above
the stage. It can be rotated clockwise or
counterclockwise to increase or decrease the
magnification. The change in magnification
results due to a change in the objective lens.
5. Objective lenses – The objective lens is the
lens that is closest to the specimen. They are
fitted on the nosepiece. A standard
microscope has 3 to 4 objective lenses of
different magnifying powers: 4X, 10X, 40X, and
100X. The objective lenses first receive the
light transmitted from the specimen and
magnify the image for the first time. Objective
lenses are color-coded and are of different
sizes. Size and color depend on the power of
the lens. The smallest lens is of the lowest
power, and gradually, the longest will be of the
highest power. The high power lenses i.e. 40X
and 100X, are retractable, i.e., their end can be
pushed inward. In most optical microscopes,
objective lenses with 100X or more
magnification are of oil immersion type.
6. The Adjustment knobs – Adjustment Knobs
are the control knobs used to focus the
:
microscope on the specimen. These knobs are
of two types;
a. Fine Adjustment Knob: Fine Adjustment
Knob is used for fine adjustment. It is a smaller
knob and is used to move the stage up or
down very slowly. The stage covers a very
small distance on each rotation of the fine
adjustment knob. It is used to sharpen the
image. It is mostly used while viewing under
high power.
b. Coarse Adjustment Knob: Coarse
Adjustment Knob is used for focusing the
image under low power magnification. It is a
larger knob and is used to move the stage up
or down very rapidly. The stage is raised or
lowered rapidly with the help of a coarse
adjustment knob.
7. Stage – This is the section in which the
specimen is placed for viewing. They have
stage clips that hold the specimen slides in
place. The most common stage is the
mechanical stage, which allows the control of
the slides by moving the slides using the
mechanical knobs on the stage instead of
moving them manually.
8. Stage Control Knobs – Stage Control Knobs
:
are the control knobs used to move the stage
mechanically. There are two knobs; one for
moving left and right and the other for moving
forward and backward. This will move the slide
in the field of vision.
9. Aperture – This is a hole in the microscope
stage through which the transmitted light from
the source reaches the stage.
10. Microscopic illuminator – A microscopic
illuminator is a light source. In some compound
microscopes, a mirror, which reflects the light
from an external source to the sample, is used.
In other optical microscopes, different electric
bulbs of low voltages are used as a constant
light source. Commonly used illuminators are
tungsten-halogen lamps, 75-150W Xenon
lamps, tin-halide lamps, mercury vapor lamps,
etc. The selection of types of bulbs is based on
the requirement of intensity and wavelength for
illumination.
11. Condenser – These are lenses that are used
to collect and focus light from the illuminator
into the specimen. They are found under the
stage next to the diaphragm of the
microscope. They play a major role in ensuring
clear, sharp images are produced with a high
:
magnification of 400X and above. The higher
the magnification of the condenser, the clearer
the image. More sophisticated microscopes
come with an Abbe condenser that has a high
magnification of about 1000X.
12. Diaphragm – It’s also known as the iris. It is
found under the stage of the microscope, and
its primary role is to control the amount of light
that reaches the specimen. It’s an adjustable
apparatus, hence controlling the light intensity
and the size of the beam of light that gets to
the specimen. For high-quality microscopes,
the diaphragm comes attached with an Abbe
condenser, and combined, they are able to
control the light focus and light intensity that
reaches the specimen.
13. Condenser focus knob – This is a knob that
moves the condenser up or down, thus
controlling the focus of light on the specimen.
14. Abbe Condenser – This condenser specially
designed for high-quality microscopes makes
the condenser movable and allows very high
magnification above 400X. High-quality
microscopes normally have a higher numerical
aperture than objective lenses.
15. The rack stop – It controls how far the stages
:
should go, preventing the objective lens from
getting too close to the specimen slide, which
may damage the specimen. It is responsible for
preventing the specimen slide from coming too
far up and hitting the objective lens.
16. Light Switch – Light Switch is an electrical
control device. Light switches are used to on
and off the illuminator.
17. Brightness Adjustment – The brightness
adjustment system controls the voltage
supplied to the light bulb, controlling the
intensity (brightness) of the light bulb.

Microscope Parts Worksheet


:
Microscope Parts Worksheet

Compound Microscope Parts


Worksheet Hints

Microscope Parts Worksheet Hints

Compound Microscope Parts


Worksheet Answer Key
:
Microscope Parts Worksheet Answer Key

Download Compound
Microscope Parts
Worksheet PDF

Light Microscope Worksheet


Answer Key
:
Inverted Microscope Worksheet
Answer Key

Dissecting microscope (Stereo


:
microscope) Worksheet
Answer Key

How Does Microscope Work?


In a simple light microscope, a thin specimen
containing a slide is placed on the microscope’s
stage.
:
A beam of light is passed through the condenser
to the specimen. The light transmitted from the
specimen enters the objective lens. While
passing through the objectives, the transmitted
rays are spread so that they appear to come from
the bigger objects.

The light is then focused on the eyepiece lens.


This lens further magnifies the pre-magnified
image coming from the objectives.

Finally, a highly magnified image can be


observed.

Parts of a Microscope Revision


Questions (FAQs)
Q. Define a Microscope.
Ans. A microscope is an optical instrument with
:
one or more lens systems that are used to get a
clear, magnified image of minute objects or
structures that can’t be viewed by the naked eye.

Q. State functions of a microscope.


Ans. A microscope is usually used for the study
of microscopic algae, fungi, and biological
specimens.

Q. Diagrammatically, identify the


various parts of a microscope.
Ans. https://microbenotes.com/wp-
content/uploads/2020/03/Microscope-Parts-
Diagram-1024×909.jpeg

Q. Describe the functions of each


part of the microscope you have
drawn above.
Ans. https://microbenotes.com/parts-of-a-
microscope/#structural-parts-of-a-microscope-
and-their-functions

Q. Differentiate between a
condenser and an Abbe condenser.
Ans. Condensers are lenses that are used to
collect and focus light from the illuminator into
the specimen. They are found under the stage
:
next to the diaphragm of the microscope. They
play a major role in ensuring clear sharp images
are produced with a high magnification of 400X
and above. Abbe condenser is a condenser
specially designed for high-quality microscopes,
which makes the condenser to be movable and
allows very high magnification of above 400X.
High-quality microscopes normally have a high
numerical aperture than objective lenses.

Q. What is the magnification power


of the objective lenses?
Ans. Objective lenses have a magnification
power of 40X to 100X.

Q. How does the eyepiece compare


to the objective lens?
Ans. The eyepiece, also known as the ocular is
the part used to look through the microscope. Its
found at the top of the microscope. Its standard
magnification is 10x with an optional eyepiece
having magnifications from 5X – 30X. Objective
Lens are the major lenses used for specimen
visualization. They have a magnification power of
40x-100x. There are about 1- 4 objective lenses
placed on one microscope, in that some are rare
facing and others face forward.
:
Q. Why is the rack stop included in
the microscope from the factory,
and can it be replaced?
Ans. Rack stop is included in the microscope for
preventing the specimen slide from coming too
far up and hitting the objective lens.

Q. What is a magnification power?


Ans. The magnification of a lens is defined as the
ratio of the height of an image to the height of an
object. Microscope magnification measures the
total enlargement of the image of an object.
Magnification power is the product of eyepiece
lens power and objective lens power.

Q. Differentiate between the fine


and the coarse adjustment knobs.
Ans. The coarse adjustment knob moves the
stage up and down to bring the specimen into
focus. The fine adjustment knob brings the
specimen into sharp focus under low power and
is used for all focusing when using high-power
lenses.

Q. List down the 18 parts of a


microscope.
1. Ocular Lens (Eye Piece)
:
2. Diopter Adjustment
3. Head
4. Nose Piece
5. Objective Lens
6. Arm (Carrying Handle)
7. Mechanical Stage
8. Stage Clip
9. Aperture
10. Diaphragm
11. Condenser
12. Coarse Adjustment
13. Fine Adjustment
14. Illuminator (Light Source)
15. Stage Controls
16. Base
17. Brightness Adjustment
18. Light Switch

Q. List down the three structural parts


of a microscope.
1. Head
2. Arms
3. Base

Q. List down the 17 parts of a


microscope.
1. Illuminator (Light Source)
:
2. Diaphragm (Iris)
3. Condenser
4. Condenser Focus Knob
5. Rack Stop
6. Stage
7. Stage Control Knobs
8. Nose Piece
9. Objective Lens
10. Tube (Head)
11. Eyepiece (Ocular Lens)
12. Diopter Adjustment
13. Adjustment Knobs (Fine Adjustment Knob and
Coarse Adjustment Knob)
14. Arm
15. Base
16. Light Switch
17. Brightness Adjustment
:
References
1. Microbiology by Lansing M. Prescott (5th
Edition)
2. https://www.pobschools.org/cms/lib/NY010014
56/Centricity/Domain/349/TheMicroscope-
howtouse.pdf
3. https://sciencing.com/parts-microscope-uses-
7431114.html
4. https://www.amscope.com/microscope-parts-
and-functions/
5. https://cpb-us-
e1.wpmucdn.com/cobblearning.net/dist/3/420
4/files/2018/08/Parts-of-the-Microscope-
103b21p.pdf
6. https://www.microscope.com/compound-
microscope-parts
7. microscope | Types, Parts, History, Diagram, &
Facts | BritannicaParts of the Microscope with
Labeling (also Free Printouts) –
Laboratoryinfo.com
8. Parts of a Microscope – The Comprehensive
Guide – Microscope and Laboratory Equipment
Reviews (microscopespot.com)
9. Microscope: Definition, Types, Uses, Parts &
Examples | Toppr
:
10. Simple Microscope Definition, Magnification,
Parts And Uses (byjus.com)
11. Phase Contrast Microscope – Definition,
Principle, Parts, Uses (learninsta.com)
12. What is Fluorescence Microscopy? Definition,
Principle, Fluorescence & Parts – Biology
Reader
13. DARK FIELD MICROSCOPE:
CHARACTERISTICS, PARTS, FUNCTIONS –
SCIENCE (warbletoncouncil.org)
14. Dissecting Stereo Microscope Parts and
Functions (microscopemaster.com)
15. Confocal Microscope Principle, Uses, Parts,
Advantages, and Disadvantages.
(microbiologynote.com)
16. Inverted microscope Images and definition,
principle, Uses, Parts. (microbiologynote.com)
17. USB Microscope- definition, principle, parts,
examples, uses.docx – USB Microscope-
definition, principle, parts, examples, uses
March 26, 2020 by Faith | Course Hero

About Author
:
Prashant Dahal

Prashant Dahal completed his


bachelor’s degree (B.Sc.)
Microbiology from Sunsari Technical
College, affiliated with Tribhuvan
University. He is interested in topics
related to Antimicrobial resistance,
the mechanism of resistance
development, Infectious diseases
(Pneumonia, tuberculosis, HIV,
malaria, dengue), Host-pathogen
interaction, Actinomycetes, fungal
metabolites, and phytochemicals as
novel sources of antimicrobials and
Vaccines.

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Solanke fatimoh omolara
August 27, 2021 at 8:40 PM

Thank you very much

Reply

Zaharaddeen
August 22, 2021 at 3:21 AM

Very good thanks

Reply

Samrau shuaibu gambo


August 19, 2021 at 7:47 PM

Thanks

Reply
:
Lilian Emmanuel
August 18, 2021 at 11:23 AM

Thank you it helps a lot but some of the


parts were not indicated.. it helps alot

Reply

Hannon
September 8, 2022 at 4:46 PM

1. which objective lens focuses


closest to object
2. what controls the light entering
the binocular lenses
3. how can you enhance the
resolving power of a microscope
4. what is useful and false
magnification
PLEASE CAN YOU HELP ME IN
ASWERING THOSE QUESTIONS

Reply
:
Tabitha sani
March 10, 2023 at 3:31 AM

Thank you so much

Reply

ISMAIL DAYYABU ZARANDA


August 13, 2021 at 2:42 AM

I LIKE IT

Reply

NDUKWE ESTHER
September 29, 2022 at 12:13 AM

understandable but small


confusion

Reply
:
Rabiu sulaiman kibiya
June 30, 2021 at 6:46 PM

Thanks this helps more

Reply

Khatem
June 11, 2021 at 4:58 PM

Very helpful!!! Thank you.

Reply

Ifechi
May 11, 2023 at 12:09 AM

Thanks much for this. We just did


microscopy as a topic and the
write-up has really helped me to
understand better. Thanks again

Reply
:
Thomas jacob
June 3, 2021 at 4:28 AM

Thank you very much for this great


information

Reply

AIMAN SHAHZADI
May 15, 2021 at 10:23 PM

Really appreciate the way all the detail


about microscope have been explained

Reply

Odepeny Silas
April 28, 2021 at 8:04 PM

Wonderful notes and really good work


done
:
Reply

ELDER
April 23, 2021 at 6:15 PM

thats good

Reply

Naveed Ullah Khan


March 10, 2021 at 8:35 PM

what are the colour codes for microscope


objective and what is the purpose of
these colour codes?

Reply

SD n pa
March 10, 2021 at 10:58 AM
:
Same here. what is the author name?

Reply

Sagar Aryal
March 10, 2021 at 5:11 PM

The author’s name is Faith


Mokobi

Reply

Marvellous
March 5, 2021 at 10:39 AM

so sweet

Reply

Aliyu tunau
March 1, 2021 at 10:08 AM
:
Really appreciate to microscope
producers, thank u

Reply

Machuei Deng Mathiang


February 24, 2021 at 6:46 PM

Thanks. It is really helpful

Reply

Machuei Deng Mathiang


February 24, 2021 at 6:42 PM

It is helpful.

Reply

angel
February 16, 2021 at 2:18 PM
:
Help me plies

Reply

Isaac Catherine
February 16, 2021 at 11:10 AM

Thanks this is very helpful

Reply

Arabiyyas
February 7, 2021 at 10:50 PM

very intresting topic it help alot

Reply

Töxïcätëd
February 5, 2021 at 12:41 AM
:
Thanks

Reply

musa jibrin
January 26, 2021 at 9:23 PM

we done with our assignment

Reply

Annoyed student
January 22, 2021 at 9:47 AM

thank you for this but their were some


parts left out like the corse adjustment
knob and the fine adjustmentknob

Reply

Devine0473
January 17, 2021 at 12:11 PM
:
This is very helpful
and highly appreciated

Reply

Yana
January 11, 2021 at 5:59 PM

Thank you!! so much help

Reply

Ajeibi John
January 7, 2021 at 8:18 PM

yes indeed it is really helpful more grace

Reply

chelseamaee
January 4, 2021 at 5:04 PM
:
thank you !

Reply

CHOL M. DENG
November 5, 2020 at 6:39 PM

Thank, it’s really helpful! I wish the writer


name was there

Reply

Max
November 11, 2021 at 3:05 AM

Thanks

Reply

Matt
November 4, 2020 at 10:57 AM
:
Thanks! This is really helpful!

Reply

Choolwe Darlingtone
November 1, 2020 at 8:20 PM

This is awesome.for studies.

Reply

Akintunde Adeniyi Best


October 16, 2020 at 5:38 AM

This is good thanks a lot

Reply

Dil Kumar Yadav


October 2, 2020 at 6:53 PM
:
It is really helpful

Reply

A
September 26, 2020 at 12:41 AM

Hi, Thanks for all the useful information!!!

Reply

Rose Johnson
September 12, 2020 at 12:43 PM

It’s good for us to study thanks you so


much

Reply

Bola Osakuade
August 13, 2020 at 7:05 AM
:
Thanks alot for this wonderful note ; it
was really helpful!!!

Reply

Ujjal sarkar
July 22, 2020 at 5:03 PM

Please author name ,books name


,published year

Reply

sadam kalyango
July 6, 2020 at 11:09 PM

It is really helpful . Giving even some self


testing questions!!!

Reply
:
subira
October 7, 2022 at 2:21 AM

good idea we can increase our


performance

Reply

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