Protein Sample set

16w 18w 20w 22w 24w

ER stress
pPERK
tPERK
pIRE
tIRE
pEIF2
tEIF2
BIP
ATF4
ATF6
CHOP
XBP1
TRAF2

Fibrotic Markers
Col1a1
aSMA

Others
CRBN
pAMPK
tAMPK

House keeping
aTubulin
Vinculin
GAPDH
Sample 18 weeks
db/+ d
1 2 3 4 5 6 1
ER stress
pPERK 3320975 3206420 2741235 2950080 3249120 4196255 2733360
tPERK
pIRE 82574 93852 76167 52962 76818 74556 13338
tIRE
pEIF2
tEIF2
BIP 85968 234756 235944 290160 399477 272844 98280
ATF4 248157 131915 302840 239240 265468 259346 275632
ATF6 36900 24120 61344 84312 67938 55944 149597
CHOP
XBP1 2778372 3669120 6354144 6831900 6659424 6390504 6760836
TRAF2 1366694 1749678 2150022 2637018 2839326 2855760 2622269

Fibrotic Markers
Col1a1
aSMA 24084 38304 70020 58428 70740 48348 51696

Others
CRBN
pAMPK
tAMPK 270846 348520 393832 543666 992640 954040 1536483

House keeping
aTubulin 1317880 3697480 6346880 4710960 11630600 10488302 6188864
Vinculin
GAPDH 182945 256865 375095 444456 487244 488480 1240668
 db/db
2 3 4 5

3308725 3612318 2838492 3821195

45917 106040 109440 249550

101340 101880 113796 115836

270543 290514 289583 269411
218595 206804 222396 189630

5393412 6372036 6449148 3990564

2155720 2763138 2940212 1930446

73800 50616 38808 54216

1893918 1985004 1191723 1218680

16265653 4213880 3830397 7751137

818160 1001943 1341560 1037655

Sample 20 weeks
db/+ d
1 2 3 4 5 6 1
ER stress
pPERK 3022192 2255118 4502862 4182170 4544631 5119957 3006471
tPERK
pIRE 77248 290813 82518 423436 185844 102068 139084
tIRE
pEIF2
tEIF2
BIP 379620 255246 249526 189126 162320 214578 95960
ATF4 427248 422997 254100 307560 346709 355653 348750
ATF6 50800 38571 29341 18108 16441 26950 95867
CHOP
XBP1 4266137 4787763 5535903 3912565 5927659 5841819 4292851
TRAF2 2182676 1417862 1995544 2021296 2180400 2203340 2125153

Fibrotic Markers
Col1a1
aSMA 60415 39560 24457 48240 58960 27475 42525

Others
CRBN
pAMPK
tAMPK 665616 829560 652785 1688526 881880 1197702 3231888

House keeping
aTubulin 3212821 4521620 3104263 5882520 7400679 10963128 5127252
Vinculin
GAPDH 177786 191360 239276 139960 250316 149205 676628
 db/db
2 3 4 5

4365240 5078206 5581600 4071360

133456 43624 105280 43168

71440 90636 168870 99288

425908 452760 717418 422418
125496 73302 114543 77742

7274903 8417587 7793478 6104910

2153730 1483160 2116569 2078659

81696 50806 98920 59120

1648584 3582374 2840680 2499978

4787240 6157133 5855169 6459012

850560 997158 1005812 619759

Sample 22 weeks
db/+
1 2 3 4 5 6 7
ER stress
pPERK
tPERK
pIRE
tIRE
pEIF2 134680 267400 214165 252385 152040 217560 76405
tEIF2 350170 348624 300636 339516 374112 769360 208404
BIP 764272 1678875 597291 872423 612535 1139896 1325414
ATF4 141064 119120 106682 90612 83435 166145 109868
ATF6
CHOP
XBP1
TRAF2 1085839 1206755 1247714 1188958 971065 1171720 910740

Fibrotic Markers
Col1a1
aSMA

Others
CRBN 4544672 4138092 3415716 3092760 3504420 5915880 4512640
pAMPK
tAMPK

House keeping
aTubulin
Vinculin 5120572 4661124 3917002 3319855 3053470 3715830 3968834
GAPDH 1670604 2032862 2059452 2217348 2513160 3312360 2611215
 db/db
1 2 3 4 5

127984 154698 181261 172480 249099

375498 394297 281304 597006 457086
318089 148703 237170 708439 385170
129409 93996 82520 113090 85897

1811333 1658832 1254825 2239400 1771938

5417888 7631040 4381552 8120866 7532480

3398265 4025119 3874212 5196234 2759895

8118026 7707560 8195544 10095668 5903482
Sample 24 weeks
db/+ d
1 2 3 4 5 6 1
ER stress
pPERK
tPERK
pIRE
tIRE
pEIF2 76067 79808 120000 97416 64800 198727 238337
tEIF2 370944 386480 306848 496320 381880 573378 571725
BIP 1286302 645228 867600 1977264 1721124 1144728 549632
ATF4 227903 137555 127257 141765 214880 197258 106969
ATF6
CHOP
XBP1
TRAF2 1139160 916145 680174 944906 1100440 893964 1418508

Fibrotic Markers
Col1a1
aSMA

Others
CRBN 5601060 3485052 3243600 4189032 5720688 3557664 3106268
pAMPK
tAMPK

House keeping
aTubulin
Vinculin 7463080 6118304 6622629 5876208 4888080 3984630 4079418
GAPDH 2788539 2190084 1949376 2593929 3240744 2947308 7265583
 db/db
2 3 4 5

172569 253055 339108 163744

616400 495938 522015 562028
579852 447192 349050 340536
94887 197327 174924 303506

1135888 1013616 956080 1468676

3974508 6069722 4983084 5230704

4140942 3799752 3119220 3207440

6804876 8538426 7138326 5956587