Microtome are devices that slice samples into incredibly tiny pieces for use in histology or

pathology. Transmitted electron microscopy(TEM) or light microscopy are used to examine the
sample. The type of microscopy depends on the type of specimen and the necessary thickness,
they employ specialized metal, gla or diamond blades. Microtome is made up of an object clamp,
a ction thickness adjustment mechanism and a blade a holding unit with a blade holder and
clamped blade .Microtome are group in to different type these types include :saw microtome,
sled microtome ,rotary microtome ,vibrating microtome and infrared laser microtome .

According to globalspec (2023) saw microtome are made to cut brittle ,hard materials like bone
and teeth .It work by pushing the sample through are cased revolving saw, they cut through the
samples. Their cut is greater than 30mm thick

The another type is a sledge microtome. This is the device where a sample is put into fixed
holder(shuttle) on a sledge microtome, which then moves back and forth across knife. Modern
sled microtome have a linear bearing on which the led is mounted ;this design enables the
microtome to quickly cut several course pieces (precisionary, 2023) .The pressure put on the
sample during the cut can be decreased by altering the angles between the samples and the
microtome knife .The preparation of big samples ,like those encased in paraffin for biological
preparations is atypical application for this type of microtome. sledge microtome can typically
achieve by cutting the thickness of 1 to 60 µm.

According to Paramedics,(2017)The rotary microtome made by microTech is a manually device

for all types of cutting device of work with paraffin and hard cutting technology in the field of
cutting technology in the field of biology medicine ,and hard cutting technology in the field of
biology medicine and industry .This type I used for microscopic preparations for subsequent
illumination(e,g human tissues. The thickness for manual operation arranges from 0.5µm to
60µm.

A vibrating blade I used in the operation of a vibrating microtome ,which enables a cut to be
made with less force than would be necessary with affixed blade .For challenging biological
samples ,the vibrating microtome is typically utilized. For lives challenging biological
biologicial samples ,the vibrating microtome is typically utilized. For live tissue ,the cut
thickness, is typically.30-500µm,while for fixed tissue ,is 10-500mm (microbeonline,2023).The
compresstome microtome is variation of the vibrating microtome , using a specimen syringe or
lipstick-like’’ tube to hold tissue ,preventing shearing, uneven cutting ,vibrating artifacts .The
compression technology does not damage or affect the tisseu being sectioned.

Infrared laser microtome are used to achieve to contact-free slicing .The only brief bursts of
infrared (IR) energy are imparted into the a sample .Laser microtomes offer several offer several
advantages including non-contact cutting ,no sample preparation native tissue ,no decalcification
of hard tissue, and a cut with precision of 1µm.They are generally too slow for routine
pathology applications (Globalspec, 2023).

To segment samples of microcopy , a variety of microtome blades are employed .Several typical
varieties of microtome blades are ,straight blades ,curved blades, serrated blades, Diamond
blades ,cryostat blades, beveled (Paramedics, 2017).

A straight blade ,These are blades that have a straight edges and are used for making thin, un
form sections of soft tissues such as brain tissue specimens (microbeonline, 2023).

The second microtome bide is curved blade .These are blades that have curved edges are used
for making thicker sections of hard tissues bone or teeth (Donald B.MacMillan, 2018).

Serrated blades is also microtome blade .these blades have saw-toothed edge and are used for
cutting through tough or fibrous tissues like cartilage ,muscle and dermis .This so due to its
feature of a saw-toothed edge (precisionary, 2023).

Diamond blade ,These are blades which are made from diamond .It is used for cutting through
very hard tissues, such as bone or teeth (microbeonline, 2023).

Beveleds blade, These are blades that have an angled edge and are used for making angled cut
through tissues (precisionary, 2023).

Last blades is cryostat blade .It specialized tool used to cut frozen tissue samples ,and thee
blades are made to be used with a cryostat (Globalspec, 2023).
The precise characteristics of the tissue being divided into section as well as the desired section
thickness will generally determine the type of blade that is utilized .

Referances

Donald B.MacMillan, R. J. (2018). chrome. In An Atlas of Comparative Vertebrate Histology (pp. ix-xxix).
Canada: Donald B.

Globalspec. (2023, 05 03). chrome. Retrieved 7 42, 2023, from Globalspec: http:www.globalspec.com

microbeonline. (2023, 04 12). chrome. Retrieved 07 23, 2023, from microbeonline:

http:microbeonline.com

Paramedics. (2017, November 19). chrome. Retrieved JULY 25, 2023, from paramedics:
http:www.paramedics.com

precisionary. (2023, february 8). chrome. Retrieved july 24, 2023, from precisionary:
http;www.precisionary.com