Analisis Obat dalam Sampel Biologis

Analisis Obat dan Makanan

 Analytical
method &
Sample data analysis
preparation
Sample
handling &
Sampling storage
techniques
Type of
specimens
Sampling techniques • Invasive
• Non invasive
Type of specimens

Jaringan
Type of specimens

Urine Saliva
Type of specimens

Whole blood
Type of specimens

Centrifuge
Type of specimens
(-) (+) Anti-coagulant
(EDTA/Heparin*)

Serum Plasma

Buffy coat
(white blood cells and platelets)

Red blood cells

Blood clot
 • Active compound stability
• Freeze-thaw stability data

Sample handling
& storage

Serum Plasma
Microtube

Freezer -80 oC
Sample preparation
Illustration figure: Solid Phase Extraction
Solid Phase Extraction
Liquid Liquid Extraction

Plasma sample 500 uL

Internal standard 50 uL*
Vortex mixer Centrifuge Supernatan
Precipitation 1 min 10000 rpm, 10 min
(MeOH/MeCN/
500 uL; TCA 10% w/v)

Evaporation Supernatan Centrifuge Vortex mixer

3000 rpm, 10 min 1 min

Reconstitution 300 uL*

 Analytical Methods
Separation-based methods
Calibration Curve
Conc. (ppm) Area Telmisartan Area Clopidogrel (IS) Peak ratio
1,0 0,50
2,0 0,98
5,0 2,45
10,0 5,05
15,0 7,80

Sample
Area Telmisartan Area Clopidogrel (IS) Peak ratio Sample conc. (ppm)
6533811 5419626 1,21 ?