AIM
Ro study and identify stages of gamete development from T.S. of testis and T.S. of ovary
through permanent slides.
DROTREMENTS
PROCED
OBSERXATIONS
T.S. of Mammalian Testis Shovs:
tunica albuginea.
1. Testis is covered by thick fibrous tissue called
interstitial tissue.
2. It contains many seminiferous tubules embedded in
tubules in the following order from
3. Various germinal cells are seen in seminiferous
periphery towards Lumen: Meiosis-I
Spermatogonia ’ Primary spermatocytes Secondary spermatocytes
(2n) (2n) (n) Meiosis-II
Spermatids ’ Spermatozoa
(n) (n)
a long tail.
The spermatozoa can be identified from their elongated head and
Biology Lab Manual-12
4. Between the germinal cells, prominent pyramid-shaped slender Sertoli cells are
provide nourishment to the developing present which
5. Interstitial tissue contains blood
sperms.
vessels and Leydig cells or Interstitial eells which
testosterone, secrete
Interstitial cell
Seminiferous tubule
Mature sperm
Sertoli cell
Spermatocytes at different
stages of spermatogenesis
Connective tissue
interstitial tissue
Fig. T.S. of testis
Interstitial cell
0) Spernmatogonial coll
Tunica albuginea
() Primary spematocyte
() Secondary sperntatocyte
(N)Sperhatu
}SpothatuZva
Seitoli cel
Biood vesset
Connectve tissue
Fig. A single seminiferous tubule enlarged
T.S. of Mammalian Ovary Shows:
VOutermost covering of ovary is germinal epithelium which lines the thick layer of fibrous tis
tunica albuginea. tissue caled
2. Stroma consists of connective tissue, blood vessels and nerve fibres.
3. Towards the periphery, stroma or cortex contains many rounded bodies called Ovarian follicles
folial or
Graafian follicles at different stages of development.
4. Each Graafian follicle has a large ovum surrounded by many layers of follicle cells.
5. Cortex may also contain a large mass of yellow cells called corpus luteum
formed after ovulatio
and it secretes progesterone.
Mesovarium
Germinal epithelium
Egg nest
Ruptured follicle with
clotted vessel (corpus
luteum)
Cumulus oophorous
Oocyte in Graafian follicle
Antrum of Graafian follicle
Ovarian stroma
Blood vessels, nerves
Different stages of
ova development
Fig. T.S. of ovary
