M.E.

S INDIAN SCHOOL, DOHA - QATAR

Section : _BOYS AND GIRLS

Class & Div. : XII SUB: BIOLOGY
PRACTICALS EXPERIMENT 3
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EXPERIMENT NO.3

a.T.S. OF TESTIS- MAMMALIAN TESTIS:

i.In T.S of testis , the seminiferous tubules appear rounded in shape surrounded by basement
membrane and lined by germinal epithelium.

ii.The seminiferous tubules are separated from each other by intertubular tissue which contains blood
vessels and interstitial cells.

iii.In between germinal cells large and prominent sertoli cells are present which provide nourishment
to the developing spermatozoa.

iv.Sperms are formed from germinal epithelium through various developmental stages like
spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa.

v.Interstitial tissue contain blood vessels and leydigcells (or) interstial cells which secrete testosterone.

DIAGRAM NCERT READER PAGE NO.44, FIG.NO.3.2

PAGE NO. 47 FIG.NO. 3.5

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b. T.S. OF MAMMALIAN OVARY:

i. Each ovary consists of a mass of connective tissue, spindle shaped cells, blood vessels and nerve
fibres , altogether forming the stroma.

ii. In the stroma egg cells are seen in various stages of development.:

Primary follicle(ovum with single layer of follicle cells) which arise from ingrowths of germinal
epithelium.

Double layered follicle (ovum with double layer of follicle cells)

The follicle slowly moves deeper into the sroma and becomes larger. A fluid filled space is formed
separating the developing follicle from other cells except at one point forming the antrum.

Mature follicle or Graafian follicle is formed by further enlargement of ovum and follicle cells.

The mature follicle migrates to the surface of ovary and ruptures to release ovum into the fallopian
tube.

After release of ovum the follicle ceels form a yellowish mass termed as corpus luteum which secretes
progesterone.

Corpus albicans is formed by the regression of corpus luteum.

DIAGRAM REFER NCERT READER PAGE.NO.49,FIG.NO.3.7

( c.T.S OF BLASTULA SHOULD BE IN THE NEXT PAGE.)

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c. T.S OF BLASTULA:

i.The blastula appears as a spherical mass of cells surrounding a cavity.

ii.The peripheral layer of cells termed as trophoblast or trophoderm which later differentiates to
form extra-embryon9ic membranes.

iii. Within the envelope there is a fluid filled cavity called blastocoel.

iv.Most of the cells inner to the trophoplast is called inner cell mass which develops into embryo.

DIAGRAM REFER PAGE NO. 52 ,FIG.NO.3.11 ( g)

F 061, Rev 01, dtd 10th March 2020