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1.0 OBJECTIVE:
To lay down the procedure for Preparation and Storage of Disinfectant Solution.
2.0 SCOPE:
This SOP is applicable for preparation and storage of disinfectant solution in the Microbiology Laboratory at
3.0 RESPONSIBILITY:
Preparation & Follow-ups : Microbiology Chemist / Officer / Executive / [Link]
Checking & Implementations : Head of the department (HOD) / Designe
Approvals : Head Quality Assurance
Authorization : Plant Head
4.0 ACCOUNTABILITY:
Head Quality control and Quality Assurance.
5.0 ABBREVATIONS:
HH :
IPA : Isopropyl Alcohol
% : Percentage
6.0 PRECAUTIONS:
6.1 Use only Validated Disinfectant.
6.2 Discard the remaining disinfectant solution (expect 70% IPA) at the end of the day operation (i.e.
After 24 hours of filtration.
6.3 Avoid direct contact of Disinfectant with eyes and skin. In case it happens, wash the eyes and
contact part with Purified Water/ Cool Water.
6.4 Prepared the disinfectant solution in purified water or water for injection.
6.5 Do not use the disinfectants after expiry.
6.6 Disinfectant, keep away from flame, source of heat and open electrical wires.
6.7 For rapid hand and skin sanitization, dispense the sufficient quantity to wet the hands and
fingernails. Rub vigorously until dry.
6.8 Always use only diluted disinfectant.
7.0 MATERIALS AND EQUIPMENTS REQUIRED:
7.1.1 Measuring Cylinder 5ml, 100ml, 1000ml.
7.1.2 Disinfectant preparation vessel.
7.1.3 Disinfectant Solution Filter. (0.22 μ)
7.1.4 Disinfectant Filtration Vessel.
7.1.5 Filtration Assembly.
8.0 PROCEDURE:
8.1 Preparation of 2.5% Dettol Solution (For Wall and Floor Cleaning):
8.1.1 Prepare the sufficient quantity of Dettol solution in purified water or water for injection.
8.1.2 For example, for preparation of 4000 ml Dettol solution:
--- PREPARED BY CHECKED BY APPROVED BY AUTHORISED BY
Designation Officer-MICRO/Designee HOD-MICRO/Designee HOD-QA/Designee Plant Head
Sign.
Date.
Name.
FORMAT NO.: HH-III/QA/001F-01/00
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8.1.3 Take 100 ml Dettol in 3900 ml purified water or water for injection in a suitable container.
8.1.4 Mix the solution uniformly with glass rod.
8.1.5 Label the container as per format no(HH-III /MIC/003/F -01/00)
8.1.6 and keep it in designated area.
8.2 Preparation of 2.5% Savlon Solution (For Wall and Floor Cleaning):
8.2.1 Prepare the sufficient quantity of Savlon solution in purified water or water for injection.
8.2.2 For example, for preparation of 4000 ml Savlon solution:
8.2.3 Take 100 ml Savlon in 3900 ml purified water or water for injection in a suitable
container.
8.2.4 Mix the solution uniformly with glass rod.
8.2.5 Label the container as per format no(HH-III /MIC/003/F -01/00)And keep it in designated
area.
8.3 Preparation of 2.5% Lizol Solution (For Wall and Floor Cleaning):
8.3.1 Prepare the sufficient quantity of Lizol solution in purified water or water for injection.
8.3.2 For example, for preparation of 4000 ml Lizol solution.
8.3.3 Take 100 ml Lizol in 3900 ml purified water or water for injection in a suitable container.
8.3.4 Mix the solution uniformly with glass rod.
8.3.5 Label the container as per format no(HH-III /MIC/003/F -01/00)and keep it in designated area.
8.4 Preparation of 70% IPA Solution (For Hand, Surface and Equipment Disinfection):
8.4.1 Prepare the sufficient quantity of IPA solution in purified water or water for injection.
8.4.2 For example, for preparation of 1000 ml IPA solution: Take 700 ml IPA in 300 ml purified water or
water for injection in a suitable container.
8.4.3 Mix the solution uniformly with glass rod.
8.4.4 Filter the solution through 0.2 µ filter and collect in a clean container.
8.4.5 Label the container as per format no. QC/MIC/017/F/02 and keep it in designated area.
8.5 Preparation of 20% Virosil Solution (For Fogging)
8.5.1 Prepare the sufficient quantity of Virosil solution in purified water or water for injection.
8.5.2 For example, for preparation of 2000 ml Virosil solution: Take 400 ml Virosil in 1600 ml purified water or
water for injection in a suitable container.
8.5.3 Mix the solution uniformly with glass rod.
8.5.4 Label the container as per format no. HH-III/MIC/003/F-02/00 and keep it in designated area.
8.6 Preparation of 2% Teepol Solution (For Glassware Cleaning):
8.6.1 Prepare the sufficient quantity of Teepol solution in purified water or water for injection.
8.6.2 For example, for preparation of 2000 ml Teepol solution: Take 40 ml Teepol in 1960 ml purified
water or water for injection in a suitable container.
8.6.3 Mix the solution uniformly with glass rod.
8.6.4 Received Disinfectant Container Label. Format No (HH-III /MIC/003/F -01/00) and keep it in
designated area.
8.7 Record the details of prepared disinfectant solution as per format [Link]-III/MIC/003/F-02/00
Disinfectant solution shall be prepared freshly on daily basis.
8.8 List of approved disinfectant Format No (HH-III /MIC/003/F -03/00
8.9 Use Dettol, Savlon and Lizol for cleaning on daily rotation.
--- PREPARED BY CHECKED BY APPROVED BY AUTHORISED BY
Designation Officer-MICRO/Designee HOD-MICRO/Designee HOD-QA/Designee Plant Head
Sign.
Date.
Name.
FORMAT NO.: HH-III/QA/001F-01/00
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9.0 ENCLOSURES:
Annexure - I Received Disinfectant Container Label. Format No (HH-III /MIC/003/F -01/00)
Annexure - II Prepared Disinfectant Container Label. Format No (HH-III /MIC/003/F -02/00)
Annexure - III List of approved disinfectant Format No (HH-III /MIC/003/F -03/00
DISTRIBUTION:
9.1 The SOP shall be distributed to respective department by QA. The QA department shall authorize
the distribution of SOP and shall sign for “Issued by”
Follow the distribution of SOP as per following:
Master Copy - QA Department (In implementation cell)
Control Copy - Microbiology Department
9.2 The revised SOP shall be distributed only after recall of superseded SOP.
10.0 HISTORY OF REVIEW:
PREVIOUS PREVIOUS REVISION
CCF NO. if any REASON FOR REVIEW
EFFECTIVE DATE NO.
Nil Nil Nil New SOP
12.0 REFERENCE
IP/BP/USP/IHS
--- PREPARED BY CHECKED BY APPROVED BY AUTHORISED BY
Designation Officer-MICRO/Designee HOD-MICRO/Designee HOD-QA/Designee Plant Head
Sign.
Date.
Name.
FORMAT NO.: HH-III/QA/001F-01/00
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ANNEXURE - I
RECEIVED DISINFECTANT CONTAINER LABEL
Name of Disinfectant
Lot/Batch No.
Container No.
Date of Receipt
Date of Opening
To be Use Before
Sign/Date
Format No. HH-III /MIC/003/F -01/00
PREPARED DISINFECTANT CONTAINER LABEL
Name of Disinfectant
Lot/Batch No.
Date of Preparation
Use Before
Prepared By
Checked By
Format No. HH-III /MIC/003/F -02/00
--- PREPARED BY CHECKED BY APPROVED BY AUTHORISED BY
Designation Officer-MICRO/Designee HOD-MICRO/Designee HOD-QA/Designee Plant Head
Sign.
Date.
Name.
FORMAT NO.: HH-III/QA/001F-01/00
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LIST OF APPROVED DISINFECTANT
Contact time
Sr. No. Name of Disinfectant Validation Concentrations To be applied on
(Minute)
01
02
03
04
05
06
07
HH-III /MIC/003/F -03/00
--- PREPARED BY CHECKED BY APPROVED BY AUTHORISED BY
Designation Officer-MICRO/Designee HOD-MICRO/Designee HOD-QA/Designee Plant Head
Sign.
Date.
Name.
FORMAT NO.: HH-III/QA/001F-01/00
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