Desi
gnandLay
outofAni
malTi
ssueCul
tur
eLabor
ator
y
Cul
tur
eLabDesi
gn
Theneedt omaint
ainasepsisdi
sti
ngui
shest
heti
ssuecult
urelaborat
oryf
rom mostot
her
s,soi
t
i
simportanti
tbedustfree.
Thei
ntroduct
ionoflami
narflowhoodshasgr
eat
lysi
mplif
iedtheproblem.
Layoutdependsont hetypeandscal
eoft heoperat
ionsandt henumberofuser
s,butsome
generalpri
ncipl
esapply.
Animaltissuecult
urelabdesi
gnforeasycl
eani
[Link]
eshouldfi
tti
ght
lyt
othefl
oororbe
suspendedf r
om thebenchwit
haspacelef
tunderneat
hf orcl
eani
ng.
Coverthefloorwi
thacov edv i
nyl
,acr
y l
i
ccoating,
orotherdustpr
ooffi
nish,andall
owaslightfal
l
i
nt heleveltowar
daf loordr ai
nlocatedoutsi
dethedooroft [Link]
ows
l
iberaluseofwaterifthefloorhastobewashed, butmoreimportant
,itpr
otectsequi
pmentfrom
damagi ngfl
oodsifsti
l
ls,autoclav
es,orsinksover
fl
ow.
Ani
mal
TissueCul
tur
eLabor
ator
y
Requi
rement
s
Mat
eri
als
Pr
ovi
sionmustbemadef
orpr
epar
ati
onandst
eri
l
izat
ion,
asept
ichandl
i
ng,
otheract
ivi
ti
eswi
thi
n
t
hecul
tur
ear
eai
ncl
udi
ngcent
ri
fugat
ion,cel
lcount
ing,mi
croscopy
,incubat
ion,andst
orageat
r
oom t
emper
atur
e,4⁰
C,−20⁰
C,and−196⁰
C.
Numberofusersdeterminehowmanylaminar
-f
lowhoodswill
berequi
red)
andwhetheral ar
gear eawi l
lbeneededtohandl
ebioreact
ors,ani
maltissuedi
ssect
ions,or
l
argenumbersofcultur
es.
Space
Thelargestar eashouldbegi ventothecul tureoperati
on,whi chhastoaccommodat elaminar-
fl
ow hoods,cel lcounters,centri
fuges,incubators,micr
oscopes,andusi ngstocksofreagents,
media,glasswar e,andpl ast
ics,and,ifpossi bl
e,aquar antineareainanimalti
ssuecult
urelab
desi
gn.
Thesecondl argestisforwashup, preparat
ion,andsteril
izati
on,thet
hir
disforstor
age,andthe
four
thisforincubat i
on.
Pr
epar
ati
onAr
ea
Facil
iti
esforwashi ngupandf orsteri
li
zat
ionshouldbelocat
ed
(a)closet otheasepticareathattheyser
v i
ceand
(b)onanout sidewalltoall
owf orthepossibi
l
ityofheatext
ract
ionf
rom ov
ensandst
eam v
ent
s
from autoclavesinanimalti
ssueculturel
abdesign.
St
orage
St
oragemustbepr
ovi
dedf
ort
hef
oll
owi
ngi
tems
(
1)St
eri
l
eli
qui
ds,
atr
oom t
emper
atur
e(sal
tsol
uti
ons,
wat
er,
etc.
),at4◦
C(medi
a),
andat−20◦
Cor
−70◦
C(ser
um,
tr
ypsi
n,et
c.)
(2)Steri
leandnonst eri
leglassware
(3)Steri
ledisposableplasti
cs(e.g.,cult
uref
lasksandPet
ri
dishes,
cent
ri
fuget
ubesandv
ial
s,and
syri
nges)
(4)Screwcaps, stoppers,etc.
(5)Apparatussuchasf i
lt
ers,steri
leandnonsteri
le
(6)Gloves,disposalbags,etc.
(7)Li
quidnitrogentor epl
enishf r
eez er
s
Access
Makesur ethatdoorwaysarewi deenoughandhighenoughandt hatcei
li
ngshavesuffi
cient
cl
earancetoallow t
hei nst
all
ati
onofequipmentsuchasl aminar
-f
low hoods,i
ncubat
ors,and
aut
[Link]
ovidespaceforaccessf
ormaint
enanceofequipment.
Steri
li
ty
Thisisoneofthemorediff
icul
tprobl
emsbecausecont ai
nmentrequi
rest
hatthecontent
soft
he
ti
ssueculturer
oom notescapetoadjacentworkareas,whil
easepsi
srequi
resthatnoneoft
he
contaminati
onofsur
roundi
ngareasentersthet
issuecult
ure.
I
ti sessent i
altohav eadedicatedti
ssueanimaltissuecul
tur
el abdesi gnwithanadj acent
preparati
onar ea,oranumberofsmalleroneswithacommonpr eparat
ionarea,rat
herthant o
havet i
ssuecul tur
eperf
ormedalongsi
deregul
arlaborator
yworkwi t
honl yalaminar-
fl
ow hood
forprotect
ion.
Aseparatefaci
li
tygi
vesbettercontaminati
onprotect
ion,al
l
owsti
ssuecul
tur
est ockst
obekept
separat
efrom regul
arl
aboratoryreagentsandglassware,andwi
ll
,inanycase,berequi
redf
or
contai
nmentifhumanorotherprimatecellsar
ehandled.
