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Choice of Bioreactor
Equipment designed to grow cells in culture
Type and design of bioreactor and mode of
operation
- Depends on cell densities and productivity
Bioreactors
Parameter - Agitation
Maximum stirring rates for suspension 100150 rpm
Microcarrier - < 40 rpm (suspension and
anchorage dependent cells)
Round bottom (animal cells) and flat
bottomed vessels (bacterial cells)
Parameter pH control
Optimal pH - 7.4 for maximum growth
Enriched Co2 atmosphere decreases pH
fluctuations
1-litre culture @ 2x106 cells/ml @ gas flow
of 100 ml/min
Parameter pH control
Direct acid or alkali addition
Net acidic production (lactic acid) from
cellular metabolism alkali (NaHCO3)
HCl is added
Computer-controlled pump or gas valve to a
pre-set pH value
Rotameters indicate rate of gas flow
controlled by flow regulators (fig-9.6)
What is Sparging?
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