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Material Safety Data Sheet of R2 – Reagent 2 (component of Ampli1™ WGA Kit)
MSDS issue date: 23rd August 2011
MSDS revision date: 23rd August 2011, revision 1.0
1. Identification of the substance or preparation
1.1. identification of the substance or preparation
Name of the product: R2 – Reagent 2.
1.2 use of the substance or preparation
The mixture is used as a base reactant for the preparation of a cell lysate reaction mixture in step 1 of the
whole genome amplification procedure carried out with Ampli1™ WGA kit. use permitted only in the
laboratory.
1.3 identification of the company responsible for placing the product in the market
Name of the Company: SILICON BIOSYSTEMS S.p.A.
Address: via dei Lapidari 12, 40129 Bologna;
Telephone: +39-051-40.71.300;
e-mail: info@siliconbiosystems.com.
1.4 Emergency telephone number
Emergency telephone number: +39-051-40.71.300;
This number is available only between 8.30 and 17.30 (GMT +1.00).
2. Identification of hazards
Xi-Irritating
The mixture can cause serious eye damage (Xi – R41). The mixture is considered as hazardous to the
aquatic environment.
3. Composition/information on ingredients
3.1 hazardous components in the preparation
The mixture contains the following hazardous ingredient:
IUPAC name of the substance: poly(oxyethylene) nonylphenyl ether;
Synonyms: nonylphenol ethoxylate;
EINECS number: 500-024-6;
CAS registry number: 9016-45-9;
Concentration present in the preparation: between 10% and 20%.
3.2 ingredients and hazard classification
The hazardous substance in the mixture is classified as follows:
IUPAC name of the substance: poly(oxyethylene) nonylphenyl ether;
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Synonyms: nonylphenol ethoxylate;
Classification: Xn;
Risk phrases: R22 – R37 – R41 – R50;
Safety phrases: S26 – S39 – S61;
Lower limit of hazardous concentration: c ≥ 10%: Xi.
Exposure limits: none (Italian law; refer to section 8.1).
4. First aid measures
General notes: symptomatic treatment should be carried out by a physician.
Following inhalation
Should the user feel discomfort and think that it is caused by the mixture, it is advisable to move him/her
away from the polluted area, take him/her outdoors and make him/her rest. Should the symptoms persist
even when the cause has been removed, seek urgent medical help.
following skin or eye contact
In the event of skin contact, should the victim experience skin irritation:
- take off all contaminated clothing;
- wash thoroughly with plenty of soap and water.
Seek medical help should the irritation persist.
In the event of eye contact, the victim may have the following symptoms: increased tearing, burning
sensation, eye discomfort or pain in the sunlight. In such a case:
- if the victim wears contact lenses, take them off;
- wash away the product as quickly as possible with plenty of water keeping the eyelids wide open.
Keep washing for at least 15 minutes.
Seek urgent medical help should the eye irritation, tearing, pain and swelling persist.
Following ingestion
Seek urgent medical help.
Do not induce vomiting unless directed to do so by a physician.
If the victim is unconscious, do not administer anything by mouth. If the victim is conscious, seek medical
attention before administering any product.
Rinse mouth immediately
5. Fire-fighting measures
5.1 suitable extinguishing media
Due to its chemical nature, the product does not burn. In the event that the mixture is involved in a fire,
use extinguishing media that are suitable for the place and for the other substances and mixtures that are
present.
5.2 extinguishing media that must not be used for safety reasons
Do not extinguish using water jets.
5.3 unusual exposure hazards due to the substance or preparation, combustion products or resulting gases.
Following combustion, fumes containing carbon oxides may be released.
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5.4 special fire-fighting equipment
Due to its chemical nature, the mixture does not require special fire-fighting equipment. To choose the
appropriate PPE, refer to the instructions provided for other substances or mixtures that are present in the
place of the fire.
6. Accidental release measures
6.1 personal precautions
Wear normal workwear and suitable PPE to prevent skin and eye contact, ingestion and inhaling.
Remove or exclude all the sources that can cause hazardous situations (e.g.: electrocution) or ignite a fire.
6.2 environmental precautions
In the event of spills, prevent the liquid from entering the sewer.
6.3 clean-up methods
In the event of small spills, dilute and remove using mechanical means (clean with a cloth) or absorb using
non-combustible material, such as sand and place everything in an appropriate container for subsequent
disposal, which must be carried out in compliance with local, regional, national and European standards in
force. Where suitable PPE to carry out these operations.
7. Handling and storage
7.1 handling
Always handle wearing appropriate gloves and workwear. Avoid placing the product in contact with hot
surfaces, naked flames and sparks.
Use only with a localised extraction system.
7.2 storage
Store the mixture in its original sealed containers at the temperature indicated on the label away from
sunlight.
7.3 specific use
Any use of the product other than that intended and authorised by SILICON BIOSYSTEMS and described in
section 1.2 is prohibited.
8. Exposure controls/personal protection
8.1 exposure limit values
Nonylphenol ethoxylate has no exposure limits established by the Italian law in force.
8.2.1 respiratory protection
Always use the product under an extraction hood.
8.2.2 hand protection
Wear lightweight nitrile gloves and dispose of them after use, in compliance with the standards in force.
8.2.3 eye protection
When needed, use normal goggles or a polycarbonate visor.
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8.2.4 skin protection
Wear normal workwear to protect skin. Always wash hands thoroughly with soap and water after using the
product, before breaks and at the end of the workshift.
8.2.5 Environmental exposure controls Address:
National and European standards concerning environment protection must be complied with when using
and disposing of the product and its containers.
9. Physical and chemical properties
appearance: colourless, slightly viscous liquid
odour: perceptible, typical
pH: approx. 5
boiling point/range: not determined
melting point/range: not determined
flash point: n.a.
flammability (solid, gas): n.a.
auto-ignition: n.a.
explosive properties: n.a.
oxidizing properties: n.a.
vapour pressure: not determined
relative density: not determined
water solubility: fully miscible
fat solubility (grease solvent to be specified): not determined
partition coefficient: n-octanol/water: not determined
viscosity: not determined
vapour density: not determined
evaporation rate: not determined
other details: no other details available
10. Stability and reactivity
10.1 conditions to avoid
Keep away from heat sources, flames and electrostatic charges and avoid exposure to direct sunlight.
10.2 materials to avoid
Avoid contact with strong acids and alkalis.
10.3 Hazardous decomposition products
No hazardous substances are known to be formed after decomposition of the product.
11. Toxicological information
Data of a study on absorption/distribution of the active principle carried out on a rat demonstrate that it
leaves the body within a few days, mainly via faeces and urine. Only a minimal part is disposed of through
exhalation, such as CO2. Studies on target organs demonstrate that greater accumulations occur on liver,
kidney, adrenal gland, thyroid, heart and spleen. Tests on rats show high absorption of the active principle
in the vaginal tissue.
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Studies on metabolism carried out on rats showed that nonylphenol ethoxylate decomposes into
nonylphenol and polyethylene glycol. Most glycol groups are carboxylated and some are metabolised in
CO2.
Acute active principle intoxication studied on rats showed tremor, ataxia, lethargy and coma.
Tests carried out on rats show that the active principle can have developmental and reproductive toxic
effects when taken orally or intravaginally (applied dose: 50 mg/Kg∙day).
There is no evidence of carcinogenicity of the active principle on rats and dogs.
11.1 inhalation exposure
There are no available data on inhalation exposure.
11.2 Ingestion exposure
LD50 (rat): 4000 mg/Kg.
When swallowed, the active principle is absorbed by the gastrointestinal tract. Symptoms of acute
ingestion exposure to the active principle are: tremor, lethargy and hepatic congestion.
11.3 exposure to skin or eye contact
LD50 (rabbit, skin): 4490 mg/Kg.
The product is irritating to skin and eyes.
There are cases in literature based on sensitisation active principle
12. Ecological information
12.1 General environmental information
Use according to good working practices and prevent dispersion of the product in the environment.
Water hazard class (German law, VwVwS; WGK = Wassergefährdungsklassen): 2.
Avoid releasing the product in the sewer or in surface waters.
12.2 ecotoxicity
Toxicity to fish:
- LC50 (Carassius auratus, 48 h): 5.4 mg/l;
- LC50 (Lepomis macrochirus, 96 h): 1 – 9.7 mg/l;
- LC50 (Oncorhynchus mykiss, 96 h): 4.1 – 5.3 mg/l;
- NOEC (Pimephales promelas, 144 h): 1.8 mg/l;
- LOEC (Pimephales promelas, 144 h): 2 mg/l.
Toxicity to algae:
- NOEC (Pseudokirchneriella subcapitata, 96 h): 8 mg/l;
- LOEC (Pseudokirchneriella subcapitata, 96 h): 16 mg/l;
Toxicity to invertebrates (aquatic environment):
- EC50 (Daphnia magna, 48 h): 12.2 – 17 mg/l;
- LOEC (Daphnia magna, 144 h): 20 mg/l.
12.3 mobility
There is no available information concerning the mobility of the mixture and its active principle.
12.4 persistence and degradability
Biodegradability of the active principle varies according to the test conditions (concentration of materials,
microorganisms involved). In general, the alkyl ethoxylate chain is degraded much faster than nonyl
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phenol, although in appropriate conditions the latter part was also degraded. Studies carried out measuring
the development of CO2 demonstrate that nonylphenol ethoxylate-9 (NPE9) is degraded by 70%.
12.5 bioaccumulative potential
Studies on bioaccumulation have been carried out on the family of nonylphenol in general and have shown
that as the number of moles oxyethylene increases, the lipophilic properties decrease, whereas the
hydrophilic increase. Therefore, bioaccumulation decreases as the moles of ethylene oxide increase.
Studies carried out on fish show bioaccumulation on gills, blood, liver, kidney, bile and bladder.
12.6 other adverse effects
There is no available information concerning other adverse effects of the mixture and its active principle.
13. Disposal considerations
Before being dumped or destroyed, the product containers must be entrusted, prior decontamination, to a
recycling and material recovery service.
Product residuals must be disposed of in compliance with local, regional, national and European standards,
taking any threshold limit value into account.
14. Transport information
14.1 special precautions
Do not place heavier packages on top of the ones that contain the Ampli1™ kit.
14.2 Transport classification according to: ADR/RID, IMDG/IMO, ICAO/IATA
The transport of the mixture is not regulated.
15. Regulatory information
National and European provisions governing the use of the product:
- Italian Legislative Decree 81/08 “Implementation of art. 1 of Italian Law No. 123 dated 3 August 2007
concerning health and safety in the workplace (Consolidated act on hygiene and safety in the workplace)”;
- Italian Legislative Decree 152/06 “Environmental standards”;
- Italian Decree of the Ministry of Labour and Social Policy dated 26 February 2004 “Definition of a first list
of indicative limit values for occupational exposure to chemicals”;
- Italian Legislative Decree 65/03 “Implementation of Directives 1999/45/EC and 2001/60/EC concerning
classification, packaging and labelling of hazardous preparations”;
- Standard 2006/1907/EC “concerning the registration, evaluation, authorisation and restriction of
chemicals (REACH), establishing a European Chemicals Agency, which amends Directive 45/1999/EC and
repeals (EEC) Council regulation 793/93 and (EC) Commission Regulation 1488/94, as well as Council
Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC e 2000/21/EC”
- Regulation 2008/1272/EC “concerning classification, labelling and packaging of substances and mixtures
amending and repealing Directives 67/548/EEC and 1999/45/EC and amending EC Regulation 1907/2006”
in its part already in force;
- Directive 1967/548/EEC “concerning the approximation of laws, regulations and administrative provisions
relating to the classification, packaging and labelling of dangerous substances ”;
- Directive 1999/45/EC “Approximation of laws, regulations and administrative provisions relating to the
classification, packaging and labelling of dangerous substances ”;
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Information on the label
Xi – Irritating
Content: nonylphenol ethoxylate, CAS 9016-45-9 / 1 50 µl vial.
R41 – risk of serious damage to eyes.
S26 – in the event of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S39 – Wear eye/face protection.
MSDS available for professional users.
16. Other information
16.1 other relevant information
R22 = Harmful if swallowed.
R37 = Irritating to airways.
R41 = Risk of serious damage to eyes.
R50 = Very toxic to aquatic organisms.
S26 = in the event of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S39 = Wear eye/face protection.
S61 = Avoid release to the environment. Refer to special instructions/safety data sheet.
The personnel using the product and managing abnormal situations and episodes involving the product
must be adequately trained in general methods for using and managing chemical products.
The product must only be used for the purposes described in section 1.2 of this MSDS. Any other use of the
product can cause effects that cannot be qualified in advance and, for this reason, are not contained in this
MSDS.
The information provided by this MSDS has been mainly collected consulting the safety data sheet of the
hazardous mixture and the European Chemical Bureau Database (http://ecb.jrc.it). Moreover, the “Hazard
Assessment Report” was also used prepared by the Chemicals Evaluation and Research Institute, Japan,
May 2007.
16.2 Information added, removed or amended after MSDS revision
This is the first edition of this MSDS.
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The information provided in this Safety Data Sheet is based on the best of our company's knowledge.
References to the risks using this product and its intrinsic characteristics, and to legislative norms and
bibliographic sources, cannot be considered fully exhaustive. The user must evaluate every further risk that
may derive from the manner and the usage conditions of the product.
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