EEMB 138
Week 3: Statistics
Name:
TA:
Practice with Statistical Methods Worksheet:
Sex and Death in the Monarch Butterfly
PROTOCOL
You have been provided with an excel spreadsheet of raw Monarch butterfly data. You will use
this sheet as your resource for three statistical tests you will be asked to perform in the Monarch
Stats Worksheet (below). Upon opening, you will notice that the data is not organized. Often in
science, data sheets aren’t perfectly organized and we need to go about doing so. In that vein, if
you are going to make headway on this worksheet you will need to organize your data as needed.
A good tool in excel for this is the SORT function. Also, you will need to find variance a few
times. The correct excel tool to do so is var.s (make sure not to use var.p!).
LAB
Step 1: Fill out the following table using what you learned in the statistics lecture and the
Monarch Butterfly raw dataset. Remember, there is an additional stats manual on Canvas! You
must have this table fully filled out to receive any credit for the following questions.
Female Male
Dead Total Number Measured
Samples Average Forewing
Length
Variance
Live Total Number Measured
Samples Average Forewing
Length
Variance
Mated Total Number Measured
Average Forewing
Length
Variance
Single Total Number Measured
Average Forewing Total (M+F)
Length
Variance
Live #
Dead #
Live + #
Dead
EEMB 138
Week 3: Statistics
Step 2: Use the above data to answer the following questions. Write cleanly and show all work
on the paper (no logical leaps!). For consistency, provide answers with two significant figures
after the decimal point.
Note: you will have to use each of the statistical tests introduced in the lecture once. If you don’t
know which one to use, remember data tests can only be used for specific questions/data. For
example, the binomial & chi-squared tests can only be used with categorical data; the t - test,
with continuous data. If you’re stuck between the binomial & chi-squared tests - think about your
categories.
Question 1 (3pts): Was the live sex ratio significantly different from random?
Statistical Test :
Provide Reasoning:
1 or 2 tail (why?):
H0:
HA:
EEMB 138
Week 3: Statistics
Question 2 (3pts): Is there a significant difference in the mean forewing lengths of mated versus
unmated males?
Statistical Test:
Provide Reasoning:
1 or 2 tail (why?):
H0:
HA:
Question 3 (1pts): The wing lengths of mated male butterflies were significantly smaller (!)
than those from the male population as a whole (“Live Samples”). Given this information,
describe the significance of your result from question 2 - According to these data, how does
mating relate to wing length in male monarch butterflies?
EEMB 138
Week 3: Statistics
Question 4 (3pts): Was mortality status independent of sex? (i.e. Were males and females dying
at the same rate?)
Statistical Test:
Provide Reasoning:
H0:
HA: