1
b.
Channatognapkgli aTecbnigue wscd to sepanst ta
and analyze mitwes csubs}antes bsted bo steinN
a mbilu phase.
hth statimary phaae unden the
Baxd on meLhaisBsed onte Bosed on he shape
hobile Phase Uhomatoarsplie bed
Absorptn Plaart hoat
Dheorsatog eaply ntomategapi Faport Uhaamatbre
Partior
chreornatoq reapy
theomatqraply
Ton- Exchange supert-triical
Cheomatoapy
+Ghel- tratior
Chnoatogap
TLCOne * PExperimet
Tn G
and * * * thin tke mtue'.
-volahls
a On
Compones
sletmoves
Eachcentn moves SuifoaCorponeto Prinúple
Sepatation
= Cormpleion -aluriiun
loyer
easiest Bictane spot slnoly
distane -tha tonoade tondueted
chnomatogapie traveled hae haring deperds
&Lauoencstationaruy absorebant
a Toil,
separatiorn
Reden and hìqhert
offnaty both en
silitaqel plastie on
n
bytieappear
rn asheet
ormpla/dstane Faon afinby phase the ateri ort -(TL)
eauh to. move phaes
dechriqul asshationarg alglas o
componenta
LRa).spoks. to to ike Don-
Statork
rotble
centain a reac?re
traveled mled
phaxe etort. aluniniun
thave) fhese wwiha material
by
Solvent
TLC Chanbe
poting ind
TLC Plate
Thin Layen of Sarople Spot
abgo rbert Mobile Phas
matriat
|Thio Layen Chnomatography
Make a tin mak tovoarede the botom o the
orth tae help a pend.
| Aply sam ple snlution to e manked apot.
* Pow the roobile phase into the TLC Uharmbert.
Placs he TLe plate nside Hhe TL Chaben is suh
a uooy hal the end marked noith penúl to be taed tools
the mobile phase.
* Keep the saple spot wel abore te mobila phase
but the plate shoud be rnm ersed ente roobie phase,
spots
4
ËMLL:
’Thin
druys, explogives , inks and bislogical sanpe bke
seira, urine,blood and others.