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Dallas County Southwestern Institute of Forensic Sciences Forensic Chemistry Section

CONTROLLED SUBSTANCES LABORATORY TRAINING MANUAL Version 2.1

Employee Receiving Training: ______________________ Training Dates: ________________ to _______________ Primary Trainer: _________________________________ Supervisor: _____________________________________ Section Chief: __________________________________ Date Training Completed: _________________________

Manual Authorized by:

Elizabeth Todd, Ph.D., Chief Forensic Chemistry Monica Lopez, Supervisor Toxicology Laboratory Chris Heartsill, Acting Quality Manager February 28, 2008

Effective Date:

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual Summary of Changes from Previous Manual Version Previous Version: Controlled Substances Training Manual, version 1.0 Current Version: Controlled Substances Training Manual, version 2.x 1. Update training form consistent with practice that completion of training is acknowledged by the trainee, the supervisor and the section chief. 2. Correction of various typographical, formatting, and grammatical errors. 3. Various non-substantive changes in wording and format to improve clarity. 4. Updated procedure names to reflect procedures listed in the new procedure manual. 5. Conversion of the hardcopy manual to an electronic form.

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Summary of Changes Version 2.0

Controlled Substances Training Manual Version 2.x Revisions and Corrections Effective Date 2/28/08 Authorized by The summary page from the Drug and Marihuana Training Checklist ELT was replaced with the Training Summary Checklist Description

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Corrections and Revisions Version 2.1

Trainee: ___________________________

Primary Trainer: ____________________

Controlled Substances Training Summary Stage 1: Basic Testing Activity Administrative Checklist EHS Checklist General Case Management Checklist Technical Training Checklist Drug Analysis Technical Training Checklist Marihuana

Completion Date

Method Proficiency: After a period of observation and training, the analyst will demonstrate proficiency in the following procedures by successfully performing a minimum of five competency assays for each of the following methods. Competency assays may be split samples, internally prepared samples of known concentration, quality control samples, or other samples of known concentration. The Controlled Substances Supervisor will review all competency assay work product including instrument output and protocol sheets. A summary of analyst responses versus target values will be retained as part of the training documentation packet. Procedure Alkaline Drug Analysis Cocaine Direct Dilution Direct Dilution IR Marihuana and THC analysis Pharmaceutical Analysis Weak Alkaline Drug Analysis Completion Date

Note: Successful completion of the above Method Proficiency also demonstrates proficiency in the Color Testing and Unknown Sample Screening procedures which are utilized throughout the Method Proficiency process. Directly Supervised Casework: A total of 25 cases representing as many of the Stage 1 procedures as possible will be worked under direct supervision of the trainer. A list of the 25 cases analyzed and the identity of the substance identified will be retained as part of the training documentation packet. Activity Performance of 25 directly supervised cases Written competency test Completion Date

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Training Summary Version 2.1

Supervised Casework: Upon successful completion of Stage 1 training, the analyst will be released to supervised casework using the Stage 1 procedures. The analyst will work in close proximity to a trainer during supervised casework. Analyst casework will be reviewed by both the trainer and a technical reviewer prior to release. Release to Supervised Casework for Stage 1 Assays: Analyst: Trainer: Supervisor: Section Chief: Date: Date: Date: Date:

Stage 2 Training: Forensic Training Activity Forensic Training in Drug Analysis Forensic Training in Marihuana Oral Competency Moot Court Completion Date

Release to Independent Casework: The analyst is released to independent casework for all procedures in which training is complete as noted on this Training Summary. Court testimony will be monitored for a minimum of the first three times and documented through the routine Court Monitoring process. Analyst: Trainer: Supervisor: Section Chief: Date: Date: Date: Date:

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Training Summary Version 2.1

Stage 3 Training: Documentation Additional Proficiency Method Proficiency: After training, the analyst will demonstrate proficiency in the following procedures by successfully performing a minimum of two competency assays for each of the following methods. Competency assays may be split samples, internally prepared samples of known concentration, quality control samples, or other samples of known concentration. The Controlled Substances Supervisor will review all competency assay work product including instrument output and protocol sheets. A summary of analyst responses versus target values will be retained as part of the training documentation packet. Procedure Acetylated Derivatives Acidic Drug Analysis LSD Analysis Miscellaneous: GHB Miscellaneous: Volatile Liquids Miscellaneous: Steroids Miscellaneous: Mushrooms/Cactus Clandestine Labs Date Competency Demonstrated

Proficiency in Off-Site Sampling is demonstrated by completion of the Off-Site Sampling Checklist. Activity Off-Site Sampling Date Competency Demonstrated

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Training Summary Version 2.1

TRAINING CHECKLIST Administrative Procedures

Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___ Attend County Orientation ___Read IFS Quality Manual ___ Read IFS Security Manual ___ Read Section Administrative Manual ___ Read IFS Code of Ethics and Conduct ___ Read Controlled Substances Procedure Manual Sections 1-12 ___ Enter time and attendance for one week ___ Review operation of the drug safe ___ Review peer review and proficiency process _____ _____ _____ _____ _____ _____ Instructor _____ _____ _____ _____ _____ _____ Date ______ ______ ______ ______ ______ ______

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___ Review QC processes: balance, pipettors, etc. _____ ___ Review Evidence Registration process _____

Institute of Forensic Sciences Controlled Substances Training Manual

Administrative Training Checklist Version 2.1

TRAINING CHECKLIST Environmental Health and Safety

Trainee: ___________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Trainee ___ Read IFS Environmental Health and Safety Manual ___ Attend Initial Safety Training ___ Receive Personal Protective Equipment Lab coat Safety Glasses ___ Safety Equipment Locations Gloves Eyewash Stations Emergency Shower First Aid Kits Fire Blankets Emergency Phone Numbers Fire Extinguishers Evacuation Rules and Routes Meet Emergency Wardens _____ Instructor _____ Date ______

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When all the areas have been checked off and initialed, the trainee, Supervisor, and Section Chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: ______________________________________ Supervisor: ___________________________________ Section Chief: _________________________________ Date: ________________ Date: ________________ Date: ________________

Institute of Forensic Sciences Controlled Substances Training Manual

Administrative Training Checklist Version 2.1

TRAINING CHECKLIST Drug Analysis Training

Trainee: ____________________________________________ (Print) Trainee will spend approximately one week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. The areas to be covered are as follows: Trainee 1. General Case Management _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______ Initials Instructor Date

___ Evidence and Laboratory Security ___ Internal Chain of Custody ___ Inspection of Evidence ___ Inventory and Marking of Evidence ___ Use of Drug Lims 2. Drug Analysis Case Management

___ Analytical Protocol forms ___ Weighing and description of evidence ___ Selection of evidence for analysis

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___ Color Testing _____ including reading of Color Testing procedure ___ Preliminary Identification of Marked Pharmaceuticals _____ including reading of Pharmaceutical Analysis ___ General approach to testing _____ including reading Unknown Sample Screening

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Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

Drug Analysis Training Checklist Version 2.1

TRAINING CHECKLIST Drug Analysis Training Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee 3. Technical Training _____ Initials: GC/MS Chemist _____ Date

___Read: GC Theory in Training Manual Chapter I-III, Basic Gas Chromatography McNair and Bonelli, Varian ___View: Capillary Installation, Restek

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___Read: Gas Chromatography/Mass Spectrometry _____ in Training Manual. Selected materials from the Finnigan training manual ___View: Mass Spectrometer Fundamentals, HP _____ ___Read: Chapter 1 pp 1-14, Glossary and Abbreviations in Interpretation of Mass Spectra, McLafferty and Turecek. _____

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___Read: Chapter 2, pp 31-64, HP5973 GC/MS _____ Operation Procedure ___Read: IR Theory in Training Manual. Chapter 1 _____ from An Infrared Spectroscopy Atlas for the Coatings Industry. ___ Become familiar with the following reference _____ materials: Clarkes Isolation and Identification of Drugs, Forensic Toxicology by Lowery and Garriott, The Merck Index, and PDR.

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Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

Drug Analysis Training Checklist Version 2.1

3.

Technical Training (cont) Trainee Initials: GC/MS Chemist Date

___GC/MS Operation (Qualitative Identification): ___ Auto Tune ___ Target Tune ___ Sequence Table ___ Generating a Spectrum ___ Adding Spectra ___ Subtracting Spectra ___ Generating a Library Search ___ Generating an Ion Chromatograph ___ Air and Water Check (leaks?) ___ Criteria for Acceptable Spectra ___ Identification of Unknown Spectra ___ Responsibilities of Drug Chemist II ___ Responsibilities of Drug Chemist III ___ IR Operation ___ Prepare sample ___ Check calibration with polystyrene ___ Run sample and identify ___GC Operation ___ Maintenance (insert, seal, septum) ___ Routine operation including: calibration standard computer operation reprocessing data

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Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

Drug Analysis Training Checklist Version 2.1

TRAINING CHECKLIST Drug Laboratory Procedures

Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. Ideally specific analyses will be performed using samples of known concentration (such as QC or proficiency samples) or specimen splitting; sample selection will be under the guidance of a supervisor. The areas to be covered are as follows: Initials Instructor

Trainee 4. Drug Analysis _____

Date

___Read and perform Alkaline Drug Analysis

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___Read and perform Weak Alkaline Drug Analysis_____ ___Read and perform Acidic Drug Analysis ___Read and perform Direct Dilution Analysis ___ Read and perform LSD Analysis ___ Read and perform Cocaine Direct Dilution ___ Read and perform Miscellaneous Materials ___ Read and perform Morphine Syrup Analysis ___ Read and perform IR Procedure ___ Read and perform Acetylated Derivatives ___ Make and evaluate a working standard _____ _____ _____ _____ _____ _____ _____ _____ _____

Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

Drug Analysis Training Checklist Version 2.1

TRAINING CHECKLIST Drug Analysis Laboratory Procedures Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee 5. Forensic Training _____ _____ _____ _____ ______ ______ Initials Instructor Date

___Read selected depositions ___ Read current Texas Drug Laws (particularly Principal Drug Laws) ___ Observe court testimony on 6 occasions:

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___ Read selected Appeals Court rulings

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Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

Drug Analysis Training Checklist Version 2.1

TRAINING CHECKLIST Drug Laboratory Procedures Marihuana and THC

Trainee: ____________________________________________ (Print) Trainee will spend approximately one week with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. The areas to be covered are as follows: Trainee Initials Instructor Date

1.

General Case Management Refer to Drug Analysis Checklist Marihuana Training _____ _____ _____ _____ ______ ______

2.

___Read Marihuana and THC Analysis ___Review Duquenois Levine color test Macroscopic analysis Microscopic analysis Seed germination GC/MS

___ Analytical Protocol Forms

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___ Weighing of Evidence & Conversion of Units _____ ___ Selection of Evidence for Analysis _____

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Marihuana and THC Analysis Version 2.1

TRAINING CHECKLIST Drug Laboratory Procedures Marihuana and THC Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee 3. Technical Training _____ _____ ______ Initials Instructor Date

___ The Value of the Duquenois Test for Cannabis A Survey. Keith Bailey. J Forensic Sci, 24(4): 817-840, Oct 1979

___ Marihuana Analysis with Recording Botanical_____ Features DJ Hauber. J Forensic Sci 37(6):1656-1661, Nov 1992. ___ A Comparative Analysis of Cannabis Material. RT Coutts. J Forensic Sci 24(2): 291-302, Apr 1979. ___ Sinsemilla. PJ Good, p 4-5. ___ Outlawing Marihuana. Marihuana: The First Twelve Thousand Years, EL Abell, p 237-247. ___ Marihuana. J Lewis. ___ Marihuana. Forensic Science Handbook, Vol 2: 87-92. ___ The Stereoscope. Forensic Science Handbook, Vol 1: 433-434. _____

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Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Marihuana and THC Analysis Version 2.1

TRAINING CHECKLIST Drug Laboratory Procedures Marihuana and THC

Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Initials Instructor

Trainee 4. Marihuana Analysis _____ _____ _____ _____

Date

___ Perform Duquenois-Levine Color Test ___ Perform Macroscopic Analysis ___ Perform Microscopic Analysis ___ Perform Marihuana Analysis (<0.25 ounces) ___ Perform Marihuana Analysis (>0.25 ounces) ___ Perform Seed Germination ___ Perform GC/MS

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Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Marihuana and THC Analysis Version 2.1

TRAINING CHECKLIST Drug Analysis Laboratory Procedures Marihuana and THC Trainee: ____________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. The areas to be covered are as follows: Trainee 5. Forensic Training _____ _____ _____ _____ _____ _____ ______ ______ ______ Initials Instructor Date

___Read selected depositions ___ Read selected Appeals Court rulings ___ Read current Texas Drug Laws

Court testimony monitoring is documented on the Drug Analysis Checklist.

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Marihuana and THC Analysis Version 2.1

TRAINING CHECKLIST Controlled Substances Training Manual Marihuana Core Sampling Trainee: ____________________________________________ (Print) Trainee will work with instructor covering all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. The areas to be covered are as follows: Trainee 1. Case Management _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ ______ ______ ______ ______ ______ ______ ______ ______ ______ Initials Instructor Date

___Read Off Site Sampling ___ Request for Core Sample ___ Chain of Custody ___ Verification of Balances ___ Inspection of Evidence & Packaging ___ Inventory and Marking of Evidence ___ Weighing of Evidence ___ Representative Samples ___ Observe processing of 1 core sample ___ Analysis of at least 1 core sample with 100 % peer review

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When all the areas have been completed and initialed, the trainee, Supervisor, and Section Chief will sign and date this form. This form will then be placed in the analysts training folder that is kept by the Quality Manager. Trainee: ______________________________________ Supervisor: ___________________________________ Section Chief: ________________________________ Date: ________________ Date: ________________ Date: ________________

Dallas County Institute of Forensic Sciences Controlled Substances Training Manual

Off Site Sampling Version 2.1

Dallas County Southwestern Institute of Forensic Sciences

Drug Evidence Registrar Training Checklist

Trainee:

Training Dates:

to

Independent access to drug vault on

by

Training was successfully completed with full release to independent work on: Employee: Primary Trainer: Supervisor: Section Chief: Date: Date: Date: Date:

Forward completed checklist to the Quality Manager.

Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

TRAINING CHECKLIST Evidence Registrar Technical Procedures Trainee:_________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. Training completion goal is three months from hire date:___________ The areas to be covered are as follows: Trainee ____ Read Procedure Manual for Controlled Substances Laboratory: Purpose _____ Evidence Management _____ Drug LIMS _____ OME Drug Evidence Storage and Disposal _____ Analysis and Reporting of Casework _____ ____ Review proper seal; inspect 10 pieces of evidence ____ Review organization of vault; pull 3 pieces of evidence ____ Observe evidence receipt and release ____ Perform evidence receipt 10 times with at least 2 DPD; note agency: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ Initials Trainer

Date

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____ Perform evidence release 10 times with at least 2 DPD; note agency:

Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

Trainee ____ Review evidence management using DrugLIMS _____ ____ Log-in 10 batches of evidence: _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____

Trainer _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____

Date _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____

____ Prepare 10 batches of evidence for release

____ Prepare evidence for release to chemists; release and receive evidence 10 times; note analysts

____ Review phone answering; take 3 phone calls ____ File Drug Laboratory reports ____ Manage OME Drug Evidence,5 times

Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

Trainee ____Search for 5 cases in DrugLims and Forvus ____Generate evidence and file folder labels ____Review process for ordering and returning records located off-site; order or return 2 cases ____Participate in vault inventory ____Review use of various forms: Evidence summary (various agencies) Drug Analysis Case Report Submitting agency log Submitting agency signature log Request of records form (off-site) Record Center request Log Core sample request form Security checklist Fax Coversheet Record of Conversation M.E. drug evidence submission form Medication Drop Log _____ _____ _____

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Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

TRAINING CHECKLIST Evidence Registrar Administrative Procedures Trainee:_________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below. Training completion goal is three months from hire date:___________ The areas to be covered are as follows: Trainee ____Attend County Orientation ____Read IFS Security Manual ____Review use of visitors log and visitor escort ____ Escort visitors on three occasions ____Read IFS Quality Manual, meet Quality Manager ____Read Section Administrative Manual ____Read Institute Code of Ethics and Conduct ____Perform time and attendance for 1 week ____Review request for time off process ____Setup and check voice mail ____ Obtain computer access: LIMS, Novel, ESS, Kronos, Forvus _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ Initials Trainer _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____

Date _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____

Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

TRAINING CHECKLIST Evidence Registrar Safety Trainee:_________________________________________ (Print) As each area of training is covered, that area will be checked off and the trainee and instructor will initial and date below.

The areas to be covered are as follows: Trainee ____Read IFS Environmental Health and Safety Manual ____Attend Safety Training ____Safety Equipment Locations: Gloves Eyewash Stations Emergency Shower First Aid Kits Fire Blankets Emergency Phone Numbers Fire Extinguishers Evacuation Rules and Routes Meet Floor Emergency Warden _____

Initials Trainer _____ Date _____

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Dallas County Institute of Forensic Sciences Controlled Substances Procedure Manual

Drug Evidence Registrar Training Checklist Version 2.1

FTIR TRAINING CHECKLIST Drug Analysis Training

Trainee: ____________________________________________ (Print) Trainee will cover all areas of analysis listed below. Instructor and trainee will initial and date the individual areas when trainee demonstrates proficiency. The areas to be covered are as follows: Trainee Initials Instructor Date

___ Read FTIR Procedure ___ Read FTIR Operation Procedure ___ Take FTIR Tutorial on Spectrum One

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___ Perform Instrument Diagnostic Checks (System suitability, Instrument Validation, Polystyrene) Determine if acceptable, print results _____ ___ Perform a Background Check Determine if acceptable, print results _____ ___ Run a Positive and Negative Control Determine if acceptable, print results _____ ___ Analyze Unknown 1 (Perform Library Search and print results) ___ Analyze Unknown 2 (Perform Library Search and print results) _____ _____ _____ ______ ______ _____ ______ _____ ______

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___ Analyze Unknown 3 (Perform Library Search and print results)

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___ Analyze Unknown 4


Dallas County Institute of Forensic Sciences Controlled Substances Laboratory 1

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FTIR Training Checklist Version 2.0

(Perform Library Search and print results)

___ Analyze Unknown 5 (Perform Library Search and print results)

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Dallas County Institute of Forensic Sciences Controlled Substances Laboratory

FTIR Training Checklist Version 2.0

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